Falsibacillus albus
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4350 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3L7JQ20|A0A3L7JQ20_9BACI NUDIX hydrolase OS=Falsibacillus albus OX=2478915 GN=D9X91_19465 PE=3 SV=1
MM1 pKa = 7.62 EE2 pKa = 4.6 KK3 pKa = 9.53 TVEE6 pKa = 4.3 VQLSPPWVTYY16 pKa = 10.68 YY17 pKa = 11.19 NEE19 pKa = 4.23 LLNTIGKK26 pKa = 9.94 DD27 pKa = 3.06 PDD29 pKa = 3.64 ATVGPLIPSDD39 pKa = 3.28 GNYY42 pKa = 9.34 IVPVMVMGDD51 pKa = 3.58 GKK53 pKa = 10.99 AEE55 pKa = 4.03 ALATLLKK62 pKa = 9.31 PTVEE66 pKa = 4.4 FGGVTLTVVVINEE79 pKa = 4.01 EE80 pKa = 4.01 QQIVSPLPCPLGVFAVADD98 pKa = 4.05 LADD101 pKa = 3.93 TALSGNPYY109 pKa = 10.18 YY110 pKa = 10.82 DD111 pKa = 3.12 QTVVKK116 pKa = 9.38 RR117 pKa = 11.84 LSPGGPNAVYY127 pKa = 9.57 PVFAPEE133 pKa = 4.43 VIQFFNDD140 pKa = 4.73 DD141 pKa = 4.25 ISNLCNNFTGVASKK155 pKa = 10.77 VFAEE159 pKa = 4.16 VMIEE163 pKa = 3.89 EE164 pKa = 4.75 LCRR167 pKa = 11.84 VSILFSTSCEE177 pKa = 4.0 EE178 pKa = 3.92
Molecular weight: 19.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.792
IPC2_protein 4.012
IPC_protein 3.935
Toseland 3.757
ProMoST 4.024
Dawson 3.897
Bjellqvist 4.101
Wikipedia 3.795
Rodwell 3.77
Grimsley 3.668
Solomon 3.884
Lehninger 3.834
Nozaki 4.012
DTASelect 4.164
Thurlkill 3.783
EMBOSS 3.808
Sillero 4.05
Patrickios 0.947
IPC_peptide 3.884
IPC2_peptide 4.024
IPC2.peptide.svr19 3.962
Protein with the highest isoelectric point:
>tr|A0A3L7K048|A0A3L7K048_9BACI RsfA family transcriptional regulator OS=Falsibacillus albus OX=2478915 GN=D9X91_09510 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.21 RR10 pKa = 11.84 KK11 pKa = 9.62 RR12 pKa = 11.84 SKK14 pKa = 9.83 VHH16 pKa = 5.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSSKK25 pKa = 9.47 TGRR28 pKa = 11.84 KK29 pKa = 8.57 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.99 VLSAA44 pKa = 4.11
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4350
0
4350
1257474
23
2797
289.1
32.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.849 ± 0.036
0.744 ± 0.012
5.277 ± 0.033
7.228 ± 0.049
4.703 ± 0.031
7.016 ± 0.038
2.245 ± 0.019
7.839 ± 0.042
7.219 ± 0.041
9.659 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.956 ± 0.017
4.377 ± 0.03
3.682 ± 0.024
3.711 ± 0.026
3.954 ± 0.027
6.311 ± 0.027
5.012 ± 0.027
6.689 ± 0.031
1.085 ± 0.016
3.445 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here