Rosistilla oblonga

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Rosistilla

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5436 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A518IN94|A0A518IN94_9BACT HTH-type transcriptional repressor NsrR OS=Rosistilla oblonga OX=2527990 GN=nsrR PE=4 SV=1
MM1 pKa = 7.43YY2 pKa = 9.27PDD4 pKa = 3.26NWEE7 pKa = 3.82LTGPVDD13 pKa = 4.92DD14 pKa = 5.64GDD16 pKa = 4.07SEE18 pKa = 5.56GYY20 pKa = 10.03VVEE23 pKa = 4.93SPTGMFFSLNRR34 pKa = 11.84YY35 pKa = 8.82AGRR38 pKa = 11.84SDD40 pKa = 3.78HH41 pKa = 7.16EE42 pKa = 4.96NILKK46 pKa = 9.31QACQAMDD53 pKa = 3.39AEE55 pKa = 4.35YY56 pKa = 11.03DD57 pKa = 3.73EE58 pKa = 5.38VEE60 pKa = 4.31SEE62 pKa = 4.6VYY64 pKa = 10.19KK65 pKa = 11.03SEE67 pKa = 3.92GAFPEE72 pKa = 4.3EE73 pKa = 4.41VGVEE77 pKa = 3.84LQFYY81 pKa = 10.02CNSLVITSLMLAMQYY96 pKa = 10.66QDD98 pKa = 5.4DD99 pKa = 4.18VLLVQMQAEE108 pKa = 4.16NRR110 pKa = 11.84DD111 pKa = 3.22FDD113 pKa = 4.06QNEE116 pKa = 4.09LVFAAMLKK124 pKa = 8.2TVRR127 pKa = 11.84DD128 pKa = 3.91GLAA131 pKa = 3.16

Molecular weight:
14.83 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A518IUP9|A0A518IUP9_9BACT Uncharacterized protein OS=Rosistilla oblonga OX=2527990 GN=Mal33_28160 PE=4 SV=1
MM1 pKa = 7.56CGVDD5 pKa = 2.67RR6 pKa = 11.84WIRR9 pKa = 11.84DD10 pKa = 3.11RR11 pKa = 11.84CQASVHH17 pKa = 5.67PLRR20 pKa = 11.84GKK22 pKa = 10.1QRR24 pKa = 11.84GGPNKK29 pKa = 10.01CLTYY33 pKa = 10.33PEE35 pKa = 4.46YY36 pKa = 10.53LYY38 pKa = 11.08KK39 pKa = 10.78LSPIGHH45 pKa = 6.77RR46 pKa = 11.84GDD48 pKa = 3.15MRR50 pKa = 11.84KK51 pKa = 9.22FRR53 pKa = 11.84IRR55 pKa = 11.84LPPLAGGAIWHH66 pKa = 6.68CWRR69 pKa = 4.63

Molecular weight:
8.08 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5436

0

5436

2136412

29

8496

393.0

43.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.321 ± 0.039

1.125 ± 0.016

6.454 ± 0.044

5.888 ± 0.032

3.591 ± 0.018

7.676 ± 0.04

2.116 ± 0.017

5.198 ± 0.023

3.43 ± 0.031

9.66 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.021

3.137 ± 0.028

5.275 ± 0.028

4.442 ± 0.025

6.543 ± 0.035

6.462 ± 0.026

5.578 ± 0.041

7.046 ± 0.03

1.446 ± 0.014

2.343 ± 0.016

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski