Rosistilla oblonga
Average proteome isoelectric point is 6.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5436 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A518IN94|A0A518IN94_9BACT HTH-type transcriptional repressor NsrR OS=Rosistilla oblonga OX=2527990 GN=nsrR PE=4 SV=1
MM1 pKa = 7.43 YY2 pKa = 9.27 PDD4 pKa = 3.26 NWEE7 pKa = 3.82 LTGPVDD13 pKa = 4.92 DD14 pKa = 5.64 GDD16 pKa = 4.07 SEE18 pKa = 5.56 GYY20 pKa = 10.03 VVEE23 pKa = 4.93 SPTGMFFSLNRR34 pKa = 11.84 YY35 pKa = 8.82 AGRR38 pKa = 11.84 SDD40 pKa = 3.78 HH41 pKa = 7.16 EE42 pKa = 4.96 NILKK46 pKa = 9.31 QACQAMDD53 pKa = 3.39 AEE55 pKa = 4.35 YY56 pKa = 11.03 DD57 pKa = 3.73 EE58 pKa = 5.38 VEE60 pKa = 4.31 SEE62 pKa = 4.6 VYY64 pKa = 10.19 KK65 pKa = 11.03 SEE67 pKa = 3.92 GAFPEE72 pKa = 4.3 EE73 pKa = 4.41 VGVEE77 pKa = 3.84 LQFYY81 pKa = 10.02 CNSLVITSLMLAMQYY96 pKa = 10.66 QDD98 pKa = 5.4 DD99 pKa = 4.18 VLLVQMQAEE108 pKa = 4.16 NRR110 pKa = 11.84 DD111 pKa = 3.22 FDD113 pKa = 4.06 QNEE116 pKa = 4.09 LVFAAMLKK124 pKa = 8.2 TVRR127 pKa = 11.84 DD128 pKa = 3.91 GLAA131 pKa = 3.16
Molecular weight: 14.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.834
IPC_protein 3.783
Toseland 3.592
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.617
Grimsley 3.503
Solomon 3.745
Lehninger 3.706
Nozaki 3.884
DTASelect 4.05
Thurlkill 3.643
EMBOSS 3.681
Sillero 3.897
Patrickios 0.947
IPC_peptide 3.745
IPC2_peptide 3.884
IPC2.peptide.svr19 3.812
Protein with the highest isoelectric point:
>tr|A0A518IUP9|A0A518IUP9_9BACT Uncharacterized protein OS=Rosistilla oblonga OX=2527990 GN=Mal33_28160 PE=4 SV=1
MM1 pKa = 7.56 CGVDD5 pKa = 2.67 RR6 pKa = 11.84 WIRR9 pKa = 11.84 DD10 pKa = 3.11 RR11 pKa = 11.84 CQASVHH17 pKa = 5.67 PLRR20 pKa = 11.84 GKK22 pKa = 10.1 QRR24 pKa = 11.84 GGPNKK29 pKa = 10.01 CLTYY33 pKa = 10.33 PEE35 pKa = 4.46 YY36 pKa = 10.53 LYY38 pKa = 11.08 KK39 pKa = 10.78 LSPIGHH45 pKa = 6.77 RR46 pKa = 11.84 GDD48 pKa = 3.15 MRR50 pKa = 11.84 KK51 pKa = 9.22 FRR53 pKa = 11.84 IRR55 pKa = 11.84 LPPLAGGAIWHH66 pKa = 6.68 CWRR69 pKa = 4.63
Molecular weight: 8.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.663
IPC_protein 10.438
Toseland 10.482
ProMoST 10.511
Dawson 10.628
Bjellqvist 10.379
Wikipedia 10.847
Rodwell 10.833
Grimsley 10.687
Solomon 10.716
Lehninger 10.687
Nozaki 10.54
DTASelect 10.35
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.57
Patrickios 10.599
IPC_peptide 10.716
IPC2_peptide 9.721
IPC2.peptide.svr19 8.555
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5436
0
5436
2136412
29
8496
393.0
43.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.321 ± 0.039
1.125 ± 0.016
6.454 ± 0.044
5.888 ± 0.032
3.591 ± 0.018
7.676 ± 0.04
2.116 ± 0.017
5.198 ± 0.023
3.43 ± 0.031
9.66 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.268 ± 0.021
3.137 ± 0.028
5.275 ± 0.028
4.442 ± 0.025
6.543 ± 0.035
6.462 ± 0.026
5.578 ± 0.041
7.046 ± 0.03
1.446 ± 0.014
2.343 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here