Acidimicrobium ferrooxidans (strain DSM 10331 / JCM 15462 / NBRC 103882 / ICP)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Acidimicrobiia; Acidimicrobiales; Acidimicrobiaceae; Acidimicrobium; Acidimicrobium ferrooxidans

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1935 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7LZI9|C7LZI9_ACIFD Holliday junction ATP-dependent DNA helicase RuvA OS=Acidimicrobium ferrooxidans (strain DSM 10331 / JCM 15462 / NBRC 103882 / ICP) OX=525909 GN=ruvA PE=3 SV=1
MM1 pKa = 7.31TVTEE5 pKa = 4.66PGRR8 pKa = 11.84VTCVPTTHH16 pKa = 6.77ACSEE20 pKa = 3.98RR21 pKa = 11.84SYY23 pKa = 11.24LLDD26 pKa = 2.94VFYY29 pKa = 10.32PSRR32 pKa = 11.84TASSTIVADD41 pKa = 3.81APVASVGGPYY51 pKa = 10.19PLIVFAAGFDD61 pKa = 4.15EE62 pKa = 5.79DD63 pKa = 3.81PSAYY67 pKa = 10.31VPLIEE72 pKa = 3.97AWVRR76 pKa = 11.84AGYY79 pKa = 9.81VVAAPRR85 pKa = 11.84FPLSSAWALATYY97 pKa = 10.2GVDD100 pKa = 3.29LHH102 pKa = 7.05DD103 pKa = 5.0AAIADD108 pKa = 3.86AFEE111 pKa = 6.29SDD113 pKa = 3.93MLNEE117 pKa = 4.83PGDD120 pKa = 3.59LSAAIGEE127 pKa = 4.19MDD129 pKa = 3.47VLARR133 pKa = 11.84SGVLAGRR140 pKa = 11.84VATDD144 pKa = 3.57DD145 pKa = 3.54VALAGQSDD153 pKa = 3.92GGDD156 pKa = 3.37VVLASADD163 pKa = 3.77NTCCAIPGVRR173 pKa = 11.84AVAVLSGAVFSPFGGSFFGSSVPMLVVQGSADD205 pKa = 3.66TVNPPSASQAIYY217 pKa = 11.15ADD219 pKa = 3.54AQAPKK224 pKa = 10.16YY225 pKa = 10.72LEE227 pKa = 3.95WLLGADD233 pKa = 3.41HH234 pKa = 7.38LGPYY238 pKa = 6.57TTEE241 pKa = 4.41GPAEE245 pKa = 3.93QAVAAVSIAFFDD257 pKa = 3.66TYY259 pKa = 10.49LRR261 pKa = 11.84QMAPSVTQVATIGQVSGVATEE282 pKa = 4.13EE283 pKa = 4.17ADD285 pKa = 3.49VDD287 pKa = 4.27GSS289 pKa = 3.63

Molecular weight: 29.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7M2F8|C7M2F8_ACIFD Peptidase M20 OS=Acidimicrobium ferrooxidans (strain DSM 10331 / JCM 15462 / NBRC 103882 / ICP) OX=525909 GN=Afer_0233 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.8GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTRR25 pKa = 11.84AGRR28 pKa = 11.84AVLKK32 pKa = 10.56ARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.54GRR39 pKa = 11.84HH40 pKa = 5.49RR41 pKa = 11.84LSAA44 pKa = 3.8

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1935

0

1935

632458

35

1518

326.9

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.805 ± 0.073

0.61 ± 0.013

5.449 ± 0.052

6.11 ± 0.058

2.716 ± 0.036

8.924 ± 0.059

2.197 ± 0.024

4.103 ± 0.042

1.272 ± 0.031

10.886 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.567 ± 0.019

1.581 ± 0.032

5.491 ± 0.038

2.554 ± 0.035

8.674 ± 0.07

5.503 ± 0.047

5.395 ± 0.045

9.936 ± 0.065

1.429 ± 0.026

1.798 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski