Vibrio phage VGJ

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Fibrovirus; Vibrio virus VGJ

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q858Q3|Q858Q3_9VIRU Putative regulator protein OS=Vibrio phage VGJ OX=223524 GN=orf154 PE=4 SV=1
MM1 pKa = 6.37VTNGLTDD8 pKa = 3.62CDD10 pKa = 3.7RR11 pKa = 11.84VCFFAEE17 pKa = 4.36FAMITALTGWGDD29 pKa = 3.56HH30 pKa = 5.71QGEE33 pKa = 4.44EE34 pKa = 4.62LRR36 pKa = 11.84SSAQGGQIPIYY47 pKa = 9.26EE48 pKa = 4.54DD49 pKa = 3.2SSPIFGDD56 pKa = 4.04CKK58 pKa = 10.85

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q858Q5|Q858Q5_9VIRU ORF104 OS=Vibrio phage VGJ OX=223524 GN=orf104 PE=4 SV=1
MM1 pKa = 7.78LIKK4 pKa = 10.76LPLLGVLSMNRR15 pKa = 11.84SEE17 pKa = 4.0MTKK20 pKa = 9.96NFVFRR25 pKa = 11.84EE26 pKa = 4.35FEE28 pKa = 4.32CGLSVEE34 pKa = 4.72EE35 pKa = 4.5AAKK38 pKa = 10.78LCFKK42 pKa = 10.01TVSEE46 pKa = 4.48VKK48 pKa = 10.2QWDD51 pKa = 3.77AGEE54 pKa = 4.75KK55 pKa = 9.98IPPICKK61 pKa = 9.68RR62 pKa = 11.84LMRR65 pKa = 11.84WHH67 pKa = 6.19SRR69 pKa = 11.84KK70 pKa = 9.28EE71 pKa = 3.83LYY73 pKa = 10.74YY74 pKa = 10.93GDD76 pKa = 4.06EE77 pKa = 3.55WWGFRR82 pKa = 11.84MEE84 pKa = 4.38GGRR87 pKa = 11.84LIFPTGDD94 pKa = 2.67RR95 pKa = 11.84VAPQQLLAAIAILQIQAPDD114 pKa = 3.52DD115 pKa = 3.8AMTRR119 pKa = 11.84SKK121 pKa = 10.76LLKK124 pKa = 9.49YY125 pKa = 9.56ARR127 pKa = 11.84AMARR131 pKa = 11.84IKK133 pKa = 10.59GIKK136 pKa = 8.89

Molecular weight: 15.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

2101

29

493

161.6

18.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.282 ± 0.824

2.38 ± 0.429

6.14 ± 0.703

4.902 ± 0.435

5.045 ± 0.285

6.235 ± 0.546

1.523 ± 0.415

7.663 ± 0.652

6.045 ± 0.551

8.425 ± 1.125

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.373

4.236 ± 0.662

5.521 ± 0.658

3.57 ± 0.322

4.569 ± 0.825

6.949 ± 0.706

5.616 ± 0.616

6.045 ± 0.508

1.38 ± 0.374

3.855 ± 0.572
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski