Hendra virus (isolate Horse/Autralia/Hendra/1994)
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|O55779|C_HENDH Protein C OS=Hendra virus (isolate Horse/Autralia/Hendra/1994) OX=928303 GN=P/V/C PE=2 SV=1
MM1 pKa = 8.2 DD2 pKa = 5.84 KK3 pKa = 11.15 LDD5 pKa = 4.35 LVNDD9 pKa = 4.02 GLDD12 pKa = 3.67 IIDD15 pKa = 6.0 FIQKK19 pKa = 7.96 NQKK22 pKa = 9.6 EE23 pKa = 4.2 IQKK26 pKa = 9.07 TYY28 pKa = 10.35 GRR30 pKa = 11.84 SSIQQPSTKK39 pKa = 10.23 DD40 pKa = 2.92 RR41 pKa = 11.84 TRR43 pKa = 11.84 AWEE46 pKa = 4.59 DD47 pKa = 3.33 FLQSTSGEE55 pKa = 4.16 HH56 pKa = 5.34 EE57 pKa = 4.04 QAEE60 pKa = 4.25 GGMPKK65 pKa = 10.18 NDD67 pKa = 3.35 GGTEE71 pKa = 3.91 GRR73 pKa = 11.84 NVEE76 pKa = 4.49 DD77 pKa = 4.08 LSSVTSSDD85 pKa = 2.88 GTIGQRR91 pKa = 11.84 VSNTRR96 pKa = 11.84 AWAEE100 pKa = 4.15 DD101 pKa = 3.71 PDD103 pKa = 5.85 DD104 pKa = 3.99 IQLDD108 pKa = 3.79 PMVTDD113 pKa = 3.77 VVYY116 pKa = 10.38 HH117 pKa = 5.5 DD118 pKa = 5.08 HH119 pKa = 7.03 GGEE122 pKa = 4.34 CTGHH126 pKa = 6.32 GPSSSPEE133 pKa = 4.11 RR134 pKa = 11.84 GWSYY138 pKa = 11.35 HH139 pKa = 4.99 MSGTHH144 pKa = 6.83 DD145 pKa = 3.6 GNVRR149 pKa = 11.84 AVPDD153 pKa = 3.84 TKK155 pKa = 10.96 VLPNAPKK162 pKa = 7.97 TTVPEE167 pKa = 4.04 EE168 pKa = 3.8 VRR170 pKa = 11.84 EE171 pKa = 3.74 IDD173 pKa = 5.55 LIGLEE178 pKa = 4.8 DD179 pKa = 3.68 KK180 pKa = 10.69 FASAGLNPAAVPFVPKK196 pKa = 10.41 NQSTPTEE203 pKa = 4.22 EE204 pKa = 4.17 PPVIPEE210 pKa = 3.8 YY211 pKa = 10.91 YY212 pKa = 9.21 YY213 pKa = 10.92 GSGRR217 pKa = 11.84 RR218 pKa = 11.84 GDD220 pKa = 3.94 LSKK223 pKa = 11.06 SPPRR227 pKa = 11.84 GNVNLDD233 pKa = 3.39 SIKK236 pKa = 10.28 IYY238 pKa = 10.63 TSDD241 pKa = 5.66 DD242 pKa = 3.27 EE243 pKa = 6.1 DD244 pKa = 4.12 EE245 pKa = 4.57 NQLEE249 pKa = 4.37 YY250 pKa = 10.84 EE251 pKa = 4.37 DD252 pKa = 5.24 EE253 pKa = 4.3 FAKK256 pKa = 10.76 SSSEE260 pKa = 4.06 VVIDD264 pKa = 3.71 TTPEE268 pKa = 4.12 DD269 pKa = 3.52 NDD271 pKa = 4.54 SINQEE276 pKa = 4.17 EE277 pKa = 4.98 VVGDD281 pKa = 3.99 PSDD284 pKa = 3.27 QGLEE288 pKa = 4.08 HH289 pKa = 7.35 PFPLGKK295 pKa = 10.19 FPEE298 pKa = 4.49 KK299 pKa = 10.9 EE300 pKa = 3.77 EE301 pKa = 4.29 TPDD304 pKa = 3.46 VRR306 pKa = 11.84 RR307 pKa = 11.84 KK308 pKa = 10.48 DD309 pKa = 3.98 SLMQDD314 pKa = 2.74 SCKK317 pKa = 10.41 RR318 pKa = 11.84 GGVPKK323 pKa = 10.14 RR324 pKa = 11.84 LPMLSEE330 pKa = 4.08 EE331 pKa = 4.65 FEE333 pKa = 4.69 CSGSDD338 pKa = 3.89 DD339 pKa = 4.82 PIIQEE344 pKa = 4.03 LEE346 pKa = 4.14 RR347 pKa = 11.84 EE348 pKa = 4.45 GSHH351 pKa = 6.9 PGGSLRR357 pKa = 11.84 LRR359 pKa = 11.84 EE360 pKa = 4.37 PPQSSGNSRR369 pKa = 11.84 NQPDD373 pKa = 3.87 RR374 pKa = 11.84 QLKK377 pKa = 8.89 TGDD380 pKa = 3.34 AASPGGVQRR389 pKa = 11.84 PGTPMPKK396 pKa = 9.92 SRR398 pKa = 11.84 IMPIKK403 pKa = 10.11 KK404 pKa = 8.71 GTDD407 pKa = 2.84 AKK409 pKa = 10.02 SQYY412 pKa = 10.86 VGTEE416 pKa = 3.95 DD417 pKa = 3.92 VPGSKK422 pKa = 9.95 SGATRR427 pKa = 11.84 YY428 pKa = 9.64 VRR430 pKa = 11.84 GLPPNQEE437 pKa = 4.37 SKK439 pKa = 11.0 SVTAEE444 pKa = 3.86 NVQLSAPSAVTRR456 pKa = 11.84 NEE458 pKa = 3.7 GHH460 pKa = 6.13 DD461 pKa = 3.58 QEE463 pKa = 4.66 VTSNEE468 pKa = 3.82 DD469 pKa = 3.47 SLDD472 pKa = 3.55 DD473 pKa = 4.32 KK474 pKa = 11.7 YY475 pKa = 11.71 IMPSDD480 pKa = 4.12 DD481 pKa = 3.54 FANTFLPHH489 pKa = 6.27 DD490 pKa = 3.98 TDD492 pKa = 3.45 RR493 pKa = 11.84 LNYY496 pKa = 9.78 HH497 pKa = 7.13 ADD499 pKa = 3.27 HH500 pKa = 6.8 LNDD503 pKa = 3.59 YY504 pKa = 10.8 DD505 pKa = 6.15 LEE507 pKa = 4.53 TLCEE511 pKa = 4.02 EE512 pKa = 4.32 SVLMGIVNAIKK523 pKa = 10.6 LINIDD528 pKa = 2.99 MRR530 pKa = 11.84 LNHH533 pKa = 6.89 IEE535 pKa = 4.08 EE536 pKa = 4.13 QMKK539 pKa = 9.97 EE540 pKa = 3.54 IPKK543 pKa = 9.95 IINKK547 pKa = 9.12 IDD549 pKa = 3.73 SIDD552 pKa = 3.62 RR553 pKa = 11.84 VLAKK557 pKa = 10.14 TNTALSTIEE566 pKa = 3.82 GHH568 pKa = 6.27 LVSMMIMIPGKK579 pKa = 10.71 GKK581 pKa = 10.41 GEE583 pKa = 4.01 RR584 pKa = 11.84 KK585 pKa = 10.06 GKK587 pKa = 8.56 TNPEE591 pKa = 4.17 LKK593 pKa = 10.27 PVIGRR598 pKa = 11.84 NILEE602 pKa = 4.11 QQEE605 pKa = 4.59 LFSFDD610 pKa = 3.12 NLKK613 pKa = 10.5 NFRR616 pKa = 11.84 DD617 pKa = 4.0 GSLTDD622 pKa = 3.4 EE623 pKa = 5.05 PYY625 pKa = 11.12 GGVARR630 pKa = 11.84 IRR632 pKa = 11.84 DD633 pKa = 3.99 DD634 pKa = 5.43 LILPEE639 pKa = 4.98 LNFSEE644 pKa = 4.7 TNASQFVPLADD655 pKa = 4.39 DD656 pKa = 3.73 ASKK659 pKa = 11.32 DD660 pKa = 3.65 VVRR663 pKa = 11.84 TMIRR667 pKa = 11.84 THH669 pKa = 5.8 IKK671 pKa = 10.32 DD672 pKa = 3.27 RR673 pKa = 11.84 EE674 pKa = 4.18 LRR676 pKa = 11.84 SEE678 pKa = 4.36 LMDD681 pKa = 3.62 YY682 pKa = 11.1 LNRR685 pKa = 11.84 AEE687 pKa = 4.16 TDD689 pKa = 3.46 EE690 pKa = 4.25 EE691 pKa = 4.33 VQEE694 pKa = 4.36 VANTVNDD701 pKa = 4.37 IIDD704 pKa = 3.68 GNII707 pKa = 2.98
Molecular weight: 78.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.681
IPC2_protein 4.571
IPC_protein 4.546
Toseland 4.393
ProMoST 4.66
Dawson 4.507
Bjellqvist 4.66
Wikipedia 4.38
Rodwell 4.393
Grimsley 4.304
Solomon 4.507
Lehninger 4.457
Nozaki 4.609
DTASelect 4.8
Thurlkill 4.393
EMBOSS 4.406
Sillero 4.673
Patrickios 4.126
IPC_peptide 4.507
IPC2_peptide 4.66
IPC2.peptide.svr19 4.609
Protein with the highest isoelectric point:
>sp|O89342|FUS_HENDH Fusion glycoprotein F0 OS=Hendra virus (isolate Horse/Autralia/Hendra/1994) OX=928303 GN=F PE=1 SV=2
MM1 pKa = 7.83 DD2 pKa = 5.04 FSVSDD7 pKa = 3.91 NLDD10 pKa = 3.55 DD11 pKa = 5.46 PIEE14 pKa = 4.41 GVSDD18 pKa = 4.4 FSPTSWEE25 pKa = 3.72 NGGYY29 pKa = 9.56 LDD31 pKa = 4.74 KK32 pKa = 11.47 VEE34 pKa = 4.56 PEE36 pKa = 3.8 IDD38 pKa = 3.15 KK39 pKa = 10.67 HH40 pKa = 8.13 GSMIPKK46 pKa = 9.78 YY47 pKa = 10.43 KK48 pKa = 10.14 IYY50 pKa = 10.47 TPGANEE56 pKa = 3.92 RR57 pKa = 11.84 KK58 pKa = 9.29 FNNYY62 pKa = 8.05 MYY64 pKa = 8.14 MICYY68 pKa = 9.91 GFVEE72 pKa = 4.43 DD73 pKa = 4.87 VEE75 pKa = 4.66 RR76 pKa = 11.84 SPEE79 pKa = 3.72 SGKK82 pKa = 10.18 RR83 pKa = 11.84 KK84 pKa = 9.73 KK85 pKa = 10.13 IRR87 pKa = 11.84 TIAAYY92 pKa = 8.88 PLGVGKK98 pKa = 8.55 STSHH102 pKa = 6.1 PQDD105 pKa = 3.82 LLEE108 pKa = 4.51 EE109 pKa = 4.4 LCSLKK114 pKa = 9.7 VTVRR118 pKa = 11.84 RR119 pKa = 11.84 TAGATEE125 pKa = 4.39 KK126 pKa = 10.48 IVFGSSGPLHH136 pKa = 6.95 HH137 pKa = 7.19 LLPWKK142 pKa = 10.39 KK143 pKa = 9.78 ILTGGSIFNAVKK155 pKa = 9.82 VCRR158 pKa = 11.84 NVDD161 pKa = 3.61 QIQLEE166 pKa = 4.4 NQQSLRR172 pKa = 11.84 IFFLSITKK180 pKa = 10.61 LNDD183 pKa = 2.92 SGIYY187 pKa = 8.39 MIPRR191 pKa = 11.84 TMLEE195 pKa = 3.67 FRR197 pKa = 11.84 RR198 pKa = 11.84 NNAIAFNLLVYY209 pKa = 10.11 LKK211 pKa = 10.15 IDD213 pKa = 3.72 ADD215 pKa = 3.97 LAKK218 pKa = 10.71 AGIQGSFDD226 pKa = 3.58 KK227 pKa = 11.15 DD228 pKa = 3.39 GTKK231 pKa = 10.06 VASFMLHH238 pKa = 6.3 LGNFVRR244 pKa = 11.84 RR245 pKa = 11.84 AGKK248 pKa = 9.46 YY249 pKa = 9.93 YY250 pKa = 10.36 SVEE253 pKa = 3.81 YY254 pKa = 10.06 CKK256 pKa = 10.67 RR257 pKa = 11.84 KK258 pKa = 8.62 IDD260 pKa = 3.75 RR261 pKa = 11.84 MKK263 pKa = 10.86 LQFSLGSIGGLSLHH277 pKa = 6.47 IKK279 pKa = 9.92 INGVISKK286 pKa = 10.48 RR287 pKa = 11.84 LFAQMGFQKK296 pKa = 10.44 NLCFSLMDD304 pKa = 3.9 INPWLNRR311 pKa = 11.84 LTWNNSCEE319 pKa = 4.12 ISRR322 pKa = 11.84 VAAVLQPSVPRR333 pKa = 11.84 EE334 pKa = 3.42 FMIYY338 pKa = 10.68 DD339 pKa = 3.94 DD340 pKa = 4.73 VFIDD344 pKa = 3.65 NTGKK348 pKa = 10.1 ILKK351 pKa = 9.78 GG352 pKa = 3.34
Molecular weight: 39.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.668
IPC2_protein 8.551
IPC_protein 8.434
Toseland 9.107
ProMoST 8.96
Dawson 9.428
Bjellqvist 9.253
Wikipedia 9.575
Rodwell 9.663
Grimsley 9.458
Solomon 9.472
Lehninger 9.443
Nozaki 9.385
DTASelect 9.151
Thurlkill 9.297
EMBOSS 9.575
Sillero 9.443
Patrickios 4.762
IPC_peptide 9.472
IPC2_peptide 7.907
IPC2.peptide.svr19 7.728
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
6056
166
2244
672.9
75.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.64 ± 0.583
1.717 ± 0.299
6.555 ± 0.603
6.489 ± 0.642
3.104 ± 0.444
6.258 ± 0.764
1.882 ± 0.307
7.48 ± 0.71
6.093 ± 0.337
9.098 ± 0.852
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.725 ± 0.268
5.234 ± 0.267
5.168 ± 0.774
3.913 ± 0.323
5.433 ± 0.406
8.438 ± 0.266
5.664 ± 0.269
5.862 ± 0.353
0.958 ± 0.146
3.286 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here