Amino acid dipepetide frequency for Bosea sp. Root483D1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.271AlaAla: 19.271 ± 0.158	1.122AlaCys: 1.122 ± 0.025	6.361AlaAsp: 6.361 ± 0.063	8.171AlaGlu: 8.171 ± 0.079	4.924AlaPhe: 4.924 ± 0.054	12.271AlaGly: 12.271 ± 0.094	2.187AlaHis: 2.187 ± 0.037	7.069AlaIle: 7.069 ± 0.063	4.355AlaLys: 4.355 ± 0.056	15.388AlaLeu: 15.388 ± 0.103	3.679AlaMet: 3.679 ± 0.044	2.915AlaAsn: 2.915 ± 0.042	6.064AlaPro: 6.064 ± 0.056	4.447AlaGln: 4.447 ± 0.051	9.435AlaArg: 9.435 ± 0.079	6.938AlaSer: 6.938 ± 0.078	6.447AlaThr: 6.447 ± 0.072	9.215AlaVal: 9.215 ± 0.075	1.563AlaTrp: 1.563 ± 0.027	2.638AlaTyr: 2.638 ± 0.041	0.002AlaXaa: 0.002 ± 0.001
Cys	0.976CysAla: 0.976 ± 0.025	0.102CysCys: 0.102 ± 0.007	0.483CysAsp: 0.483 ± 0.016	0.443CysGlu: 0.443 ± 0.016	0.32CysPhe: 0.32 ± 0.014	0.911CysGly: 0.911 ± 0.023	0.224CysHis: 0.224 ± 0.01	0.403CysIle: 0.403 ± 0.014	0.178CysLys: 0.178 ± 0.01	0.805CysLeu: 0.805 ± 0.019	0.132CysMet: 0.132 ± 0.008	0.17CysAsn: 0.17 ± 0.01	0.383CysPro: 0.383 ± 0.015	0.203CysGln: 0.203 ± 0.012	0.619CysArg: 0.619 ± 0.017	0.436CysSer: 0.436 ± 0.015	0.359CysThr: 0.359 ± 0.014	0.574CysVal: 0.574 ± 0.021	0.117CysTrp: 0.117 ± 0.009	0.175CysTyr: 0.175 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	6.493AspAla: 6.493 ± 0.06	0.439AspCys: 0.439 ± 0.016	2.7AspAsp: 2.7 ± 0.045	3.369AspGlu: 3.369 ± 0.048	2.055AspPhe: 2.055 ± 0.033	5.212AspGly: 5.212 ± 0.053	1.061AspHis: 1.061 ± 0.024	2.793AspIle: 2.793 ± 0.044	1.647AspLys: 1.647 ± 0.036	5.691AspLeu: 5.691 ± 0.061	1.087AspMet: 1.087 ± 0.021	1.03AspAsn: 1.03 ± 0.023	3.368AspPro: 3.368 ± 0.047	1.49AspGln: 1.49 ± 0.025	3.889AspArg: 3.889 ± 0.043	1.921AspSer: 1.921 ± 0.033	2.13AspThr: 2.13 ± 0.039	3.696AspVal: 3.696 ± 0.047	0.947AspTrp: 0.947 ± 0.025	1.329AspTyr: 1.329 ± 0.03	0.002AspXaa: 0.002 ± 0.001
Glu	8.35GluAla: 8.35 ± 0.086	0.323GluCys: 0.323 ± 0.013	2.324GluAsp: 2.324 ± 0.04	2.857GluGlu: 2.857 ± 0.047	1.705GluPhe: 1.705 ± 0.031	4.152GluGly: 4.152 ± 0.048	1.125GluHis: 1.125 ± 0.027	3.581GluIle: 3.581 ± 0.045	2.004GluLys: 2.004 ± 0.03	5.675GluLeu: 5.675 ± 0.061	1.341GluMet: 1.341 ± 0.027	1.273GluAsn: 1.273 ± 0.025	3.042GluPro: 3.042 ± 0.046	2.036GluGln: 2.036 ± 0.036	5.431GluArg: 5.431 ± 0.066	2.202GluSer: 2.202 ± 0.039	3.294GluThr: 3.294 ± 0.04	3.726GluVal: 3.726 ± 0.051	0.598GluTrp: 0.598 ± 0.017	0.802GluTyr: 0.802 ± 0.02	0.001GluXaa: 0.001 ± 0.001
Phe	5.091PheAla: 5.091 ± 0.054	0.404PheCys: 0.404 ± 0.014	2.5PheAsp: 2.5 ± 0.038	2.053PheGlu: 2.053 ± 0.037	1.37PhePhe: 1.37 ± 0.031	3.899PheGly: 3.899 ± 0.045	0.746PheHis: 0.746 ± 0.022	1.659PheIle: 1.659 ± 0.03	1.045PheLys: 1.045 ± 0.026	3.378PheLeu: 3.378 ± 0.05	0.787PheMet: 0.787 ± 0.02	0.981PheAsn: 0.981 ± 0.025	1.637PhePro: 1.637 ± 0.027	1.024PheGln: 1.024 ± 0.022	2.321PheArg: 2.321 ± 0.036	2.174PheSer: 2.174 ± 0.039	1.849PheThr: 1.849 ± 0.031	2.917PheVal: 2.917 ± 0.051	0.563PheTrp: 0.563 ± 0.018	0.838PheTyr: 0.838 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	10.198GlyAla: 10.198 ± 0.089	0.85GlyCys: 0.85 ± 0.022	4.215GlyAsp: 4.215 ± 0.062	5.202GlyGlu: 5.202 ± 0.054	3.896GlyPhe: 3.896 ± 0.043	7.757GlyGly: 7.757 ± 0.093	1.883GlyHis: 1.883 ± 0.034	4.584GlyIle: 4.584 ± 0.049	3.442GlyLys: 3.442 ± 0.05	10.147GlyLeu: 10.147 ± 0.082	2.213GlyMet: 2.213 ± 0.035	2.076GlyAsn: 2.076 ± 0.044	3.787GlyPro: 3.787 ± 0.046	3.013GlyGln: 3.013 ± 0.045	6.629GlyArg: 6.629 ± 0.068	4.886GlySer: 4.886 ± 0.063	4.524GlyThr: 4.524 ± 0.069	6.127GlyVal: 6.127 ± 0.058	1.416GlyTrp: 1.416 ± 0.028	2.277GlyTyr: 2.277 ± 0.042	0.002GlyXaa: 0.002 ± 0.001
His	2.299HisAla: 2.299 ± 0.034	0.217HisCys: 0.217 ± 0.011	1.176HisAsp: 1.176 ± 0.028	1.022HisGlu: 1.022 ± 0.027	0.757HisPhe: 0.757 ± 0.024	1.928HisGly: 1.928 ± 0.032	0.516HisHis: 0.516 ± 0.016	0.82HisIle: 0.82 ± 0.022	0.448HisLys: 0.448 ± 0.016	1.98HisLeu: 1.98 ± 0.032	0.409HisMet: 0.409 ± 0.015	0.382HisAsn: 0.382 ± 0.015	1.238HisPro: 1.238 ± 0.029	0.521HisGln: 0.521 ± 0.018	1.352HisArg: 1.352 ± 0.026	0.802HisSer: 0.802 ± 0.025	0.687HisThr: 0.687 ± 0.019	1.465HisVal: 1.465 ± 0.026	0.327HisTrp: 0.327 ± 0.013	0.493HisTyr: 0.493 ± 0.014	0.001HisXaa: 0.001 ± 0.001
Ile	8.145IleAla: 8.145 ± 0.072	0.498IleCys: 0.498 ± 0.019	3.269IleAsp: 3.269 ± 0.039	3.519IleGlu: 3.519 ± 0.042	1.583IlePhe: 1.583 ± 0.028	5.341IleGly: 5.341 ± 0.061	0.823IleHis: 0.823 ± 0.021	2.088IleIle: 2.088 ± 0.036	1.429IleLys: 1.429 ± 0.03	4.383IleLeu: 4.383 ± 0.056	0.938IleMet: 0.938 ± 0.021	1.2IleAsn: 1.2 ± 0.023	2.252IlePro: 2.252 ± 0.039	1.136IleGln: 1.136 ± 0.023	3.121IleArg: 3.121 ± 0.038	2.621IleSer: 2.621 ± 0.038	2.512IleThr: 2.512 ± 0.038	4.609IleVal: 4.609 ± 0.046	0.629IleTrp: 0.629 ± 0.017	1.011IleTyr: 1.011 ± 0.023	0.001IleXaa: 0.001 ± 0.001
Lys	4.741LysAla: 4.741 ± 0.056	0.124LysCys: 0.124 ± 0.008	1.554LysAsp: 1.554 ± 0.037	1.456LysGlu: 1.456 ± 0.032	0.806LysPhe: 0.806 ± 0.023	2.706LysGly: 2.706 ± 0.041	0.545LysHis: 0.545 ± 0.018	1.522LysIle: 1.522 ± 0.033	1.056LysLys: 1.056 ± 0.03	3.656LysLeu: 3.656 ± 0.05	0.614LysMet: 0.614 ± 0.018	0.696LysAsn: 0.696 ± 0.021	2.283LysPro: 2.283 ± 0.042	1.061LysGln: 1.061 ± 0.024	2.4LysArg: 2.4 ± 0.042	1.611LysSer: 1.611 ± 0.034	1.813LysThr: 1.813 ± 0.033	2.432LysVal: 2.432 ± 0.042	0.296LysTrp: 0.296 ± 0.013	0.502LysTyr: 0.502 ± 0.017	0.001LysXaa: 0.001 ± 0.0
Leu	16.095LeuAla: 16.095 ± 0.11	0.901LeuCys: 0.901 ± 0.025	6.206LeuAsp: 6.206 ± 0.065	4.829LeuGlu: 4.829 ± 0.053	3.72LeuPhe: 3.72 ± 0.053	9.309LeuGly: 9.309 ± 0.084	1.747LeuHis: 1.747 ± 0.033	5.393LeuIle: 5.393 ± 0.059	3.758LeuLys: 3.758 ± 0.05	10.16LeuLeu: 10.16 ± 0.096	2.348LeuMet: 2.348 ± 0.035	2.403LeuAsn: 2.403 ± 0.032	6.053LeuPro: 6.053 ± 0.062	2.733LeuGln: 2.733 ± 0.04	7.19LeuArg: 7.19 ± 0.07	6.624LeuSer: 6.624 ± 0.065	5.738LeuThr: 5.738 ± 0.063	8.08LeuVal: 8.08 ± 0.064	1.224LeuTrp: 1.224 ± 0.024	1.944LeuTyr: 1.944 ± 0.036	0.002LeuXaa: 0.002 ± 0.001
Met	3.073MetAla: 3.073 ± 0.041	0.126MetCys: 0.126 ± 0.008	0.933MetAsp: 0.933 ± 0.024	0.89MetGlu: 0.89 ± 0.021	0.615MetPhe: 0.615 ± 0.019	1.593MetGly: 1.593 ± 0.03	0.375MetHis: 0.375 ± 0.013	1.256MetIle: 1.256 ± 0.025	0.857MetLys: 0.857 ± 0.021	2.622MetLeu: 2.622 ± 0.036	0.565MetMet: 0.565 ± 0.017	0.671MetAsn: 0.671 ± 0.019	1.492MetPro: 1.492 ± 0.029	0.793MetGln: 0.793 ± 0.023	1.793MetArg: 1.793 ± 0.034	1.564MetSer: 1.564 ± 0.028	1.768MetThr: 1.768 ± 0.031	1.659MetVal: 1.659 ± 0.026	0.182MetTrp: 0.182 ± 0.01	0.268MetTyr: 0.268 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.985AsnAla: 2.985 ± 0.044	0.208AsnCys: 0.208 ± 0.011	1.213AsnAsp: 1.213 ± 0.033	1.098AsnGlu: 1.098 ± 0.027	0.857AsnPhe: 0.857 ± 0.02	2.213AsnGly: 2.213 ± 0.043	0.438AsnHis: 0.438 ± 0.014	1.116AsnIle: 1.116 ± 0.024	0.614AsnLys: 0.614 ± 0.016	2.536AsnLeu: 2.536 ± 0.036	0.478AsnMet: 0.478 ± 0.015	0.63AsnAsn: 0.63 ± 0.02	1.73AsnPro: 1.73 ± 0.031	0.73AsnGln: 0.73 ± 0.022	1.68AsnArg: 1.68 ± 0.029	1.084AsnSer: 1.084 ± 0.027	1.098AsnThr: 1.098 ± 0.026	1.717AsnVal: 1.717 ± 0.038	0.42AsnTrp: 0.42 ± 0.014	0.588AsnTyr: 0.588 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	7.326ProAla: 7.326 ± 0.075	0.306ProCys: 0.306 ± 0.015	3.348ProAsp: 3.348 ± 0.044	3.688ProGlu: 3.688 ± 0.05	2.077ProPhe: 2.077 ± 0.038	4.835ProGly: 4.835 ± 0.051	1.0ProHis: 1.0 ± 0.022	2.372ProIle: 2.372 ± 0.036	1.683ProLys: 1.683 ± 0.035	5.128ProLeu: 5.128 ± 0.055	1.182ProMet: 1.182 ± 0.026	1.348ProAsn: 1.348 ± 0.028	2.667ProPro: 2.667 ± 0.055	1.785ProGln: 1.785 ± 0.029	3.206ProArg: 3.206 ± 0.044	2.821ProSer: 2.821 ± 0.043	2.484ProThr: 2.484 ± 0.034	4.313ProVal: 4.313 ± 0.047	0.694ProTrp: 0.694 ± 0.019	1.147ProTyr: 1.147 ± 0.024	0.002ProXaa: 0.002 ± 0.001
Gln	4.608GlnAla: 4.608 ± 0.056	0.189GlnCys: 0.189 ± 0.01	1.401GlnAsp: 1.401 ± 0.028	1.417GlnGlu: 1.417 ± 0.029	0.969GlnPhe: 0.969 ± 0.025	2.522GlnGly: 2.522 ± 0.036	0.611GlnHis: 0.611 ± 0.019	1.682GlnIle: 1.682 ± 0.032	0.989GlnLys: 0.989 ± 0.023	2.975GlnLeu: 2.975 ± 0.038	0.761GlnMet: 0.761 ± 0.019	0.812GlnAsn: 0.812 ± 0.022	1.885GlnPro: 1.885 ± 0.037	1.24GlnGln: 1.24 ± 0.033	2.634GlnArg: 2.634 ± 0.046	1.619GlnSer: 1.619 ± 0.026	1.606GlnThr: 1.606 ± 0.029	2.251GlnVal: 2.251 ± 0.036	0.365GlnTrp: 0.365 ± 0.014	0.513GlnTyr: 0.513 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	8.22ArgAla: 8.22 ± 0.069	0.522ArgCys: 0.522 ± 0.018	3.995ArgAsp: 3.995 ± 0.045	4.394ArgGlu: 4.394 ± 0.055	3.193ArgPhe: 3.193 ± 0.042	5.05ArgGly: 5.05 ± 0.05	1.676ArgHis: 1.676 ± 0.034	4.032ArgIle: 4.032 ± 0.044	2.231ArgLys: 2.231 ± 0.037	8.477ArgLeu: 8.477 ± 0.075	1.749ArgMet: 1.749 ± 0.029	1.781ArgAsn: 1.781 ± 0.03	3.763ArgPro: 3.763 ± 0.055	2.651ArgGln: 2.651 ± 0.039	5.971ArgArg: 5.971 ± 0.07	3.925ArgSer: 3.925 ± 0.043	3.272ArgThr: 3.272 ± 0.044	4.61ArgVal: 4.61 ± 0.05	1.04ArgTrp: 1.04 ± 0.024	1.697ArgTyr: 1.697 ± 0.03	0.001ArgXaa: 0.001 ± 0.001
Ser	6.488SerAla: 6.488 ± 0.062	0.411SerCys: 0.411 ± 0.014	2.659SerAsp: 2.659 ± 0.036	2.774SerGlu: 2.774 ± 0.033	2.466SerPhe: 2.466 ± 0.036	5.575SerGly: 5.575 ± 0.07	1.034SerHis: 1.034 ± 0.025	2.521SerIle: 2.521 ± 0.043	1.473SerLys: 1.473 ± 0.028	5.727SerLeu: 5.727 ± 0.059	1.138SerMet: 1.138 ± 0.028	1.207SerAsn: 1.207 ± 0.028	2.892SerPro: 2.892 ± 0.041	1.583SerGln: 1.583 ± 0.027	3.554SerArg: 3.554 ± 0.046	2.796SerSer: 2.796 ± 0.05	2.581SerThr: 2.581 ± 0.038	3.805SerVal: 3.805 ± 0.051	0.808SerTrp: 0.808 ± 0.022	1.267SerTyr: 1.267 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	6.31ThrAla: 6.31 ± 0.064	0.375ThrCys: 0.375 ± 0.015	2.29ThrAsp: 2.29 ± 0.038	2.426ThrGlu: 2.426 ± 0.04	1.796ThrPhe: 1.796 ± 0.032	5.003ThrGly: 5.003 ± 0.062	0.913ThrHis: 0.913 ± 0.024	3.0ThrIle: 3.0 ± 0.045	1.371ThrLys: 1.371 ± 0.03	6.027ThrLeu: 6.027 ± 0.07	1.159ThrMet: 1.159 ± 0.024	1.158ThrAsn: 1.158 ± 0.03	3.222ThrPro: 3.222 ± 0.045	1.498ThrGln: 1.498 ± 0.03	3.316ThrArg: 3.316 ± 0.039	2.706ThrSer: 2.706 ± 0.044	2.824ThrThr: 2.824 ± 0.041	4.109ThrVal: 4.109 ± 0.05	0.591ThrTrp: 0.591 ± 0.019	1.093ThrTyr: 1.093 ± 0.023	0.002ThrXaa: 0.002 ± 0.001
Val	10.002ValAla: 10.002 ± 0.073	0.569ValCys: 0.569 ± 0.021	3.842ValAsp: 3.842 ± 0.048	4.524ValGlu: 4.524 ± 0.05	2.794ValPhe: 2.794 ± 0.042	5.789ValGly: 5.789 ± 0.066	1.234ValHis: 1.234 ± 0.027	3.894ValIle: 3.894 ± 0.049	2.317ValLys: 2.317 ± 0.039	7.674ValLeu: 7.674 ± 0.078	1.838ValMet: 1.838 ± 0.034	1.788ValAsn: 1.788 ± 0.038	3.832ValPro: 3.832 ± 0.046	1.913ValGln: 1.913 ± 0.033	4.729ValArg: 4.729 ± 0.051	4.194ValSer: 4.194 ± 0.053	4.485ValThr: 4.485 ± 0.051	6.062ValVal: 6.062 ± 0.061	0.781ValTrp: 0.781 ± 0.019	1.346ValTyr: 1.346 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.238TrpAla: 1.238 ± 0.027	0.133TrpCys: 0.133 ± 0.009	0.56TrpAsp: 0.56 ± 0.018	0.529TrpGlu: 0.529 ± 0.017	0.536TrpPhe: 0.536 ± 0.017	0.905TrpGly: 0.905 ± 0.021	0.305TrpHis: 0.305 ± 0.012	0.596TrpIle: 0.596 ± 0.016	0.412TrpLys: 0.412 ± 0.015	1.816TrpLeu: 1.816 ± 0.034	0.304TrpMet: 0.304 ± 0.012	0.424TrpAsn: 0.424 ± 0.016	0.752TrpPro: 0.752 ± 0.021	0.559TrpGln: 0.559 ± 0.019	1.245TrpArg: 1.245 ± 0.024	0.825TrpSer: 0.825 ± 0.021	0.786TrpThr: 0.786 ± 0.019	0.705TrpVal: 0.705 ± 0.02	0.239TrpTrp: 0.239 ± 0.011	0.284TrpTyr: 0.284 ± 0.01	0.001TrpXaa: 0.001 ± 0.0
Tyr	2.551TyrAla: 2.551 ± 0.037	0.195TyrCys: 0.195 ± 0.01	1.321TyrAsp: 1.321 ± 0.026	1.18TyrGlu: 1.18 ± 0.027	0.794TyrPhe: 0.794 ± 0.021	2.15TyrGly: 2.15 ± 0.041	0.397TyrHis: 0.397 ± 0.016	0.725TyrIle: 0.725 ± 0.019	0.566TyrLys: 0.566 ± 0.021	2.212TyrLeu: 2.212 ± 0.035	0.372TyrMet: 0.372 ± 0.013	0.487TyrAsn: 0.487 ± 0.016	1.069TyrPro: 1.069 ± 0.021	0.632TyrGln: 0.632 ± 0.02	1.732TyrArg: 1.732 ± 0.032	1.012TyrSer: 1.012 ± 0.026	0.931TyrThr: 0.931 ± 0.023	1.537TyrVal: 1.537 ± 0.03	0.35TyrTrp: 0.35 ± 0.014	0.521TyrTyr: 0.521 ± 0.021	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.002XaaAla: 0.002 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.003XaaGly: 0.003 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.002XaaPro: 0.002 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.001XaaTyr: 0.001 ± 0.001	0.003XaaXaa: 0.003 ± 0.002

Statistics based on 6046 proteins (1907448 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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