Fusarium poae mitovirus 2
Average proteome isoelectric point is 8.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4ZA48|A0A1B4ZA48_9VIRU RNA-dependent RNA polymerase OS=Fusarium poae mitovirus 2 OX=1848151 PE=4 SV=1
MM1 pKa = 7.68 CPLKK5 pKa = 10.95 GLTHH9 pKa = 7.33 RR10 pKa = 11.84 FGGMHH15 pKa = 5.25 ITSRR19 pKa = 11.84 YY20 pKa = 7.9 VFMLITYY27 pKa = 9.0 CGRR30 pKa = 11.84 NKK32 pKa = 10.61 LLIIYY37 pKa = 7.36 MKK39 pKa = 8.36 NTKK42 pKa = 9.99 IIIVLIKK49 pKa = 10.43 RR50 pKa = 11.84 LILGIFGVDD59 pKa = 3.08 HH60 pKa = 6.79 TKK62 pKa = 11.05 LITEE66 pKa = 4.69 FVKK69 pKa = 10.42 EE70 pKa = 4.17 VQKK73 pKa = 11.14 LWINNGFMYY82 pKa = 9.7 MISYY86 pKa = 8.11 MKK88 pKa = 10.17 VVRR91 pKa = 11.84 LHH93 pKa = 4.94 ITRR96 pKa = 11.84 YY97 pKa = 9.17 VCGKK101 pKa = 9.03 PLYY104 pKa = 10.39 SNASNVSLDD113 pKa = 3.28 SSGFPTRR120 pKa = 11.84 FLFLKK125 pKa = 10.46 HH126 pKa = 6.45 LLDD129 pKa = 4.99 EE130 pKa = 5.27 GDD132 pKa = 3.5 TGIKK136 pKa = 9.74 IVFTLLSLTRR146 pKa = 11.84 GLKK149 pKa = 7.51 PTKK152 pKa = 10.79 AEE154 pKa = 4.12 DD155 pKa = 3.33 KK156 pKa = 10.54 KK157 pKa = 10.94 IKK159 pKa = 10.22 YY160 pKa = 9.92 DD161 pKa = 4.07 LGPIIAPHH169 pKa = 6.91 KK170 pKa = 7.73 GTTMGSVPGWFIKK183 pKa = 10.63 EE184 pKa = 3.54 FVRR187 pKa = 11.84 RR188 pKa = 11.84 NNLGRR193 pKa = 11.84 KK194 pKa = 8.68 LPEE197 pKa = 3.97 YY198 pKa = 10.23 SVKK201 pKa = 10.75 DD202 pKa = 3.39 HH203 pKa = 6.28 YY204 pKa = 11.44 LSTKK208 pKa = 9.95 GGPSGKK214 pKa = 8.31 STWASQWSHH223 pKa = 6.52 LFYY226 pKa = 10.94 KK227 pKa = 10.26 QDD229 pKa = 4.6 LILSLAYY236 pKa = 9.65 ILGEE240 pKa = 3.98 GFKK243 pKa = 10.55 EE244 pKa = 3.94 LFYY247 pKa = 11.11 TPFLKK252 pKa = 10.67 NMHH255 pKa = 6.72 LSYY258 pKa = 11.29 GKK260 pKa = 10.08 DD261 pKa = 2.5 RR262 pKa = 11.84 WPNGKK267 pKa = 9.96 LSIVKK272 pKa = 10.22 DD273 pKa = 3.7 PEE275 pKa = 3.83 GKK277 pKa = 9.77 RR278 pKa = 11.84 RR279 pKa = 11.84 VIAMVDD285 pKa = 3.33 YY286 pKa = 10.65 HH287 pKa = 6.73 SQLALRR293 pKa = 11.84 KK294 pKa = 8.81 VHH296 pKa = 6.99 EE297 pKa = 5.51 DD298 pKa = 3.32 LLSMLGKK305 pKa = 10.44 FSTDD309 pKa = 2.5 RR310 pKa = 11.84 TFSQNPRR317 pKa = 11.84 HH318 pKa = 5.41 NWNYY322 pKa = 10.06 NNSEE326 pKa = 4.24 LFYY329 pKa = 11.28 SLDD332 pKa = 3.5 LSSATDD338 pKa = 3.73 RR339 pKa = 11.84 FPVRR343 pKa = 11.84 LQARR347 pKa = 11.84 LVGEE351 pKa = 4.02 IYY353 pKa = 10.47 GNHH356 pKa = 6.54 RR357 pKa = 11.84 FGEE360 pKa = 4.15 QWANLLLNRR369 pKa = 11.84 DD370 pKa = 3.61 YY371 pKa = 10.91 MDD373 pKa = 4.76 PEE375 pKa = 4.77 GNNCRR380 pKa = 11.84 YY381 pKa = 10.08 AVGQPMGAYY390 pKa = 9.89 SSWAAFTLTHH400 pKa = 6.71 HH401 pKa = 6.57 LTVAWSAYY409 pKa = 8.91 KK410 pKa = 10.27 ARR412 pKa = 11.84 KK413 pKa = 7.42 TMGFDD418 pKa = 3.13 QYY420 pKa = 11.18 IILGDD425 pKa = 4.34 DD426 pKa = 3.46 IVIKK430 pKa = 10.18 DD431 pKa = 3.45 NRR433 pKa = 11.84 IAEE436 pKa = 4.23 IYY438 pKa = 8.68 KK439 pKa = 9.16 GQMMRR444 pKa = 11.84 MGVDD448 pKa = 3.13 ISLPKK453 pKa = 9.24 THH455 pKa = 6.39 VSVDD459 pKa = 3.13 TYY461 pKa = 11.34 EE462 pKa = 4.03 FAKK465 pKa = 10.39 RR466 pKa = 11.84 WIKK469 pKa = 10.27 KK470 pKa = 9.61 DD471 pKa = 3.55 RR472 pKa = 11.84 EE473 pKa = 4.03 ITGIPLKK480 pKa = 10.97 GILNNINNLKK490 pKa = 10.13 IVFTILNDD498 pKa = 3.77 YY499 pKa = 10.33 LIKK502 pKa = 10.87 CPTSVPKK509 pKa = 10.77 SSWQIFQSIFLGFQIRR525 pKa = 11.84 GGSKK529 pKa = 9.23 SRR531 pKa = 11.84 KK532 pKa = 6.84 TRR534 pKa = 11.84 TITKK538 pKa = 9.89 KK539 pKa = 10.18 YY540 pKa = 10.55 LEE542 pKa = 4.23 NLRR545 pKa = 11.84 DD546 pKa = 3.44 FAISVRR552 pKa = 11.84 YY553 pKa = 9.78 SMNLISPYY561 pKa = 8.38 EE562 pKa = 3.79 LRR564 pKa = 11.84 AYY566 pKa = 9.27 FASKK570 pKa = 10.61 VKK572 pKa = 10.53 LIKK575 pKa = 9.36 EE576 pKa = 4.18 TSDD579 pKa = 3.46 YY580 pKa = 11.3 QSIPSEE586 pKa = 4.13 KK587 pKa = 10.6 LILQYY592 pKa = 10.18 MEE594 pKa = 6.05 GILTNGLAKK603 pKa = 10.11 ISKK606 pKa = 8.73 DD607 pKa = 3.42 TIIQINKK614 pKa = 9.09 QLDD617 pKa = 3.61 SFDD620 pKa = 4.37 RR621 pKa = 11.84 LAKK624 pKa = 9.41 EE625 pKa = 4.5 DD626 pKa = 3.88 KK627 pKa = 10.5 RR628 pKa = 11.84 SLVYY632 pKa = 10.68 SGVLHH637 pKa = 6.66 GLMNRR642 pKa = 11.84 LEE644 pKa = 4.45 RR645 pKa = 11.84 LQEE648 pKa = 3.65 LCQRR652 pKa = 11.84 MKK654 pKa = 11.25 DD655 pKa = 3.39 EE656 pKa = 4.63 GSTVVEE662 pKa = 5.45 FINHH666 pKa = 4.99 FTAPSVDD673 pKa = 3.28 SLSRR677 pKa = 11.84 KK678 pKa = 9.93 DD679 pKa = 3.25 RR680 pKa = 11.84 DD681 pKa = 3.28 INIRR685 pKa = 11.84 MAFLDD690 pKa = 4.07 SLWKK694 pKa = 10.48 KK695 pKa = 10.37 SLQKK699 pKa = 10.59 HH700 pKa = 5.85 FSDD703 pKa = 4.24 QRR705 pKa = 11.84 FPDD708 pKa = 3.13 SYY710 pKa = 11.23 YY711 pKa = 11.01 RR712 pKa = 11.84 NLEE715 pKa = 3.59 TRR717 pKa = 11.84 TLYY720 pKa = 11.08 GSLDD724 pKa = 3.51 ALDD727 pKa = 4.56 IIDD730 pKa = 4.04 YY731 pKa = 10.13 EE732 pKa = 4.66 GEE734 pKa = 3.99 IEE736 pKa = 5.22 VRR738 pKa = 11.84 PIWSRR743 pKa = 11.84 EE744 pKa = 3.45 LSQFNQRR751 pKa = 11.84 SINSLQMFISEE762 pKa = 4.22 NSS764 pKa = 3.17
Molecular weight: 88.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.878
IPC2_protein 9.107
IPC_protein 9.004
Toseland 9.677
ProMoST 9.443
Dawson 9.94
Bjellqvist 9.633
Wikipedia 10.131
Rodwell 10.277
Grimsley 10.028
Solomon 9.955
Lehninger 9.911
Nozaki 9.706
DTASelect 9.619
Thurlkill 9.78
EMBOSS 10.101
Sillero 9.853
Patrickios 5.181
IPC_peptide 9.955
IPC2_peptide 8.185
IPC2.peptide.svr19 8.114
Protein with the highest isoelectric point:
>tr|A0A1B4ZA48|A0A1B4ZA48_9VIRU RNA-dependent RNA polymerase OS=Fusarium poae mitovirus 2 OX=1848151 PE=4 SV=1
MM1 pKa = 7.68 CPLKK5 pKa = 10.95 GLTHH9 pKa = 7.33 RR10 pKa = 11.84 FGGMHH15 pKa = 5.25 ITSRR19 pKa = 11.84 YY20 pKa = 7.9 VFMLITYY27 pKa = 9.0 CGRR30 pKa = 11.84 NKK32 pKa = 10.61 LLIIYY37 pKa = 7.36 MKK39 pKa = 8.36 NTKK42 pKa = 9.99 IIIVLIKK49 pKa = 10.43 RR50 pKa = 11.84 LILGIFGVDD59 pKa = 3.08 HH60 pKa = 6.79 TKK62 pKa = 11.05 LITEE66 pKa = 4.69 FVKK69 pKa = 10.42 EE70 pKa = 4.17 VQKK73 pKa = 11.14 LWINNGFMYY82 pKa = 9.7 MISYY86 pKa = 8.11 MKK88 pKa = 10.17 VVRR91 pKa = 11.84 LHH93 pKa = 4.94 ITRR96 pKa = 11.84 YY97 pKa = 9.17 VCGKK101 pKa = 9.03 PLYY104 pKa = 10.39 SNASNVSLDD113 pKa = 3.28 SSGFPTRR120 pKa = 11.84 FLFLKK125 pKa = 10.46 HH126 pKa = 6.45 LLDD129 pKa = 4.99 EE130 pKa = 5.27 GDD132 pKa = 3.5 TGIKK136 pKa = 9.74 IVFTLLSLTRR146 pKa = 11.84 GLKK149 pKa = 7.51 PTKK152 pKa = 10.79 AEE154 pKa = 4.12 DD155 pKa = 3.33 KK156 pKa = 10.54 KK157 pKa = 10.94 IKK159 pKa = 10.22 YY160 pKa = 9.92 DD161 pKa = 4.07 LGPIIAPHH169 pKa = 6.91 KK170 pKa = 7.73 GTTMGSVPGWFIKK183 pKa = 10.63 EE184 pKa = 3.54 FVRR187 pKa = 11.84 RR188 pKa = 11.84 NNLGRR193 pKa = 11.84 KK194 pKa = 8.68 LPEE197 pKa = 3.97 YY198 pKa = 10.23 SVKK201 pKa = 10.75 DD202 pKa = 3.39 HH203 pKa = 6.28 YY204 pKa = 11.44 LSTKK208 pKa = 9.95 GGPSGKK214 pKa = 8.31 STWASQWSHH223 pKa = 6.52 LFYY226 pKa = 10.94 KK227 pKa = 10.26 QDD229 pKa = 4.6 LILSLAYY236 pKa = 9.65 ILGEE240 pKa = 3.98 GFKK243 pKa = 10.55 EE244 pKa = 3.94 LFYY247 pKa = 11.11 TPFLKK252 pKa = 10.67 NMHH255 pKa = 6.72 LSYY258 pKa = 11.29 GKK260 pKa = 10.08 DD261 pKa = 2.5 RR262 pKa = 11.84 WPNGKK267 pKa = 9.96 LSIVKK272 pKa = 10.22 DD273 pKa = 3.7 PEE275 pKa = 3.83 GKK277 pKa = 9.77 RR278 pKa = 11.84 RR279 pKa = 11.84 VIAMVDD285 pKa = 3.33 YY286 pKa = 10.65 HH287 pKa = 6.73 SQLALRR293 pKa = 11.84 KK294 pKa = 8.81 VHH296 pKa = 6.99 EE297 pKa = 5.51 DD298 pKa = 3.32 LLSMLGKK305 pKa = 10.44 FSTDD309 pKa = 2.5 RR310 pKa = 11.84 TFSQNPRR317 pKa = 11.84 HH318 pKa = 5.41 NWNYY322 pKa = 10.06 NNSEE326 pKa = 4.24 LFYY329 pKa = 11.28 SLDD332 pKa = 3.5 LSSATDD338 pKa = 3.73 RR339 pKa = 11.84 FPVRR343 pKa = 11.84 LQARR347 pKa = 11.84 LVGEE351 pKa = 4.02 IYY353 pKa = 10.47 GNHH356 pKa = 6.54 RR357 pKa = 11.84 FGEE360 pKa = 4.15 QWANLLLNRR369 pKa = 11.84 DD370 pKa = 3.61 YY371 pKa = 10.91 MDD373 pKa = 4.76 PEE375 pKa = 4.77 GNNCRR380 pKa = 11.84 YY381 pKa = 10.08 AVGQPMGAYY390 pKa = 9.89 SSWAAFTLTHH400 pKa = 6.71 HH401 pKa = 6.57 LTVAWSAYY409 pKa = 8.91 KK410 pKa = 10.27 ARR412 pKa = 11.84 KK413 pKa = 7.42 TMGFDD418 pKa = 3.13 QYY420 pKa = 11.18 IILGDD425 pKa = 4.34 DD426 pKa = 3.46 IVIKK430 pKa = 10.18 DD431 pKa = 3.45 NRR433 pKa = 11.84 IAEE436 pKa = 4.23 IYY438 pKa = 8.68 KK439 pKa = 9.16 GQMMRR444 pKa = 11.84 MGVDD448 pKa = 3.13 ISLPKK453 pKa = 9.24 THH455 pKa = 6.39 VSVDD459 pKa = 3.13 TYY461 pKa = 11.34 EE462 pKa = 4.03 FAKK465 pKa = 10.39 RR466 pKa = 11.84 WIKK469 pKa = 10.27 KK470 pKa = 9.61 DD471 pKa = 3.55 RR472 pKa = 11.84 EE473 pKa = 4.03 ITGIPLKK480 pKa = 10.97 GILNNINNLKK490 pKa = 10.13 IVFTILNDD498 pKa = 3.77 YY499 pKa = 10.33 LIKK502 pKa = 10.87 CPTSVPKK509 pKa = 10.77 SSWQIFQSIFLGFQIRR525 pKa = 11.84 GGSKK529 pKa = 9.23 SRR531 pKa = 11.84 KK532 pKa = 6.84 TRR534 pKa = 11.84 TITKK538 pKa = 9.89 KK539 pKa = 10.18 YY540 pKa = 10.55 LEE542 pKa = 4.23 NLRR545 pKa = 11.84 DD546 pKa = 3.44 FAISVRR552 pKa = 11.84 YY553 pKa = 9.78 SMNLISPYY561 pKa = 8.38 EE562 pKa = 3.79 LRR564 pKa = 11.84 AYY566 pKa = 9.27 FASKK570 pKa = 10.61 VKK572 pKa = 10.53 LIKK575 pKa = 9.36 EE576 pKa = 4.18 TSDD579 pKa = 3.46 YY580 pKa = 11.3 QSIPSEE586 pKa = 4.13 KK587 pKa = 10.6 LILQYY592 pKa = 10.18 MEE594 pKa = 6.05 GILTNGLAKK603 pKa = 10.11 ISKK606 pKa = 8.73 DD607 pKa = 3.42 TIIQINKK614 pKa = 9.09 QLDD617 pKa = 3.61 SFDD620 pKa = 4.37 RR621 pKa = 11.84 LAKK624 pKa = 9.41 EE625 pKa = 4.5 DD626 pKa = 3.88 KK627 pKa = 10.5 RR628 pKa = 11.84 SLVYY632 pKa = 10.68 SGVLHH637 pKa = 6.66 GLMNRR642 pKa = 11.84 LEE644 pKa = 4.45 RR645 pKa = 11.84 LQEE648 pKa = 3.65 LCQRR652 pKa = 11.84 MKK654 pKa = 11.25 DD655 pKa = 3.39 EE656 pKa = 4.63 GSTVVEE662 pKa = 5.45 FINHH666 pKa = 4.99 FTAPSVDD673 pKa = 3.28 SLSRR677 pKa = 11.84 KK678 pKa = 9.93 DD679 pKa = 3.25 RR680 pKa = 11.84 DD681 pKa = 3.28 INIRR685 pKa = 11.84 MAFLDD690 pKa = 4.07 SLWKK694 pKa = 10.48 KK695 pKa = 10.37 SLQKK699 pKa = 10.59 HH700 pKa = 5.85 FSDD703 pKa = 4.24 QRR705 pKa = 11.84 FPDD708 pKa = 3.13 SYY710 pKa = 11.23 YY711 pKa = 11.01 RR712 pKa = 11.84 NLEE715 pKa = 3.59 TRR717 pKa = 11.84 TLYY720 pKa = 11.08 GSLDD724 pKa = 3.51 ALDD727 pKa = 4.56 IIDD730 pKa = 4.04 YY731 pKa = 10.13 EE732 pKa = 4.66 GEE734 pKa = 3.99 IEE736 pKa = 5.22 VRR738 pKa = 11.84 PIWSRR743 pKa = 11.84 EE744 pKa = 3.45 LSQFNQRR751 pKa = 11.84 SINSLQMFISEE762 pKa = 4.22 NSS764 pKa = 3.17
Molecular weight: 88.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.878
IPC2_protein 9.107
IPC_protein 9.004
Toseland 9.677
ProMoST 9.443
Dawson 9.94
Bjellqvist 9.633
Wikipedia 10.131
Rodwell 10.277
Grimsley 10.028
Solomon 9.955
Lehninger 9.911
Nozaki 9.706
DTASelect 9.619
Thurlkill 9.78
EMBOSS 10.101
Sillero 9.853
Patrickios 5.181
IPC_peptide 9.955
IPC2_peptide 8.185
IPC2.peptide.svr19 8.114
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
764
764
764
764.0
88.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.534 ± 0.0
0.785 ± 0.0
5.366 ± 0.0
4.319 ± 0.0
4.843 ± 0.0
6.283 ± 0.0
2.487 ± 0.0
8.246 ± 0.0
8.115 ± 0.0
10.995 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.01 ± 0.0
4.843 ± 0.0
3.272 ± 0.0
3.141 ± 0.0
6.283 ± 0.0
8.246 ± 0.0
5.105 ± 0.0
4.581 ± 0.0
1.702 ± 0.0
4.843 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here