Neisseria weaveri
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2011 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A448VPT1|A0A448VPT1_9NEIS 50S ribosomal protein L19 OS=Neisseria weaveri OX=28091 GN=rplS PE=3 SV=1
MM1 pKa = 7.63 SDD3 pKa = 3.37 EE4 pKa = 4.26 SPIIFTEE11 pKa = 4.37 SCCAKK16 pKa = 10.45 VADD19 pKa = 6.17 LIAEE23 pKa = 4.23 EE24 pKa = 4.56 NNPDD28 pKa = 3.05 LKK30 pKa = 11.19 LRR32 pKa = 11.84 VFVNGGGCSGFQYY45 pKa = 10.94 GFTFDD50 pKa = 5.34 EE51 pKa = 4.5 ITNDD55 pKa = 3.97 DD56 pKa = 3.87 DD57 pKa = 6.11 FEE59 pKa = 4.65 IKK61 pKa = 10.2 KK62 pKa = 10.71 DD63 pKa = 3.46 GLTFLVDD70 pKa = 3.61 PMSYY74 pKa = 10.26 QYY76 pKa = 11.24 LIGAEE81 pKa = 3.7 IDD83 pKa = 3.78 YY84 pKa = 10.37 TEE86 pKa = 4.57 SLQGSQFVIRR96 pKa = 11.84 NPNATTTCGCGSSFSVV112 pKa = 3.54
Molecular weight: 12.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.859
IPC_protein 3.795
Toseland 3.592
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.719
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.656
EMBOSS 3.732
Sillero 3.91
Patrickios 0.54
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A3S4Z9W3|A0A3S4Z9W3_9NEIS Histidinol-phosphate aminotransferase OS=Neisseria weaveri OX=28091 GN=hisC PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.43 RR14 pKa = 11.84 THH16 pKa = 5.89 GFLVRR21 pKa = 11.84 SKK23 pKa = 9.38 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2011
0
2011
633418
30
3743
315.0
34.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.0 ± 0.071
0.957 ± 0.02
5.247 ± 0.062
6.189 ± 0.055
3.913 ± 0.049
7.51 ± 0.076
2.26 ± 0.029
5.768 ± 0.042
5.482 ± 0.051
10.129 ± 0.1
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.505 ± 0.034
4.261 ± 0.063
4.195 ± 0.044
4.354 ± 0.044
5.106 ± 0.052
5.66 ± 0.047
5.206 ± 0.074
7.066 ± 0.06
1.265 ± 0.026
2.927 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here