Streptococcus minor
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1898 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3P1VBG5|A0A3P1VBG5_9STRE Uncharacterized protein OS=Streptococcus minor OX=229549 GN=EII38_04900 PE=4 SV=1
MM1 pKa = 7.27 SVTVKK6 pKa = 10.22 VDD8 pKa = 3.63 YY9 pKa = 11.02 YY10 pKa = 11.78 SSLCDD15 pKa = 2.93 SFMYY19 pKa = 10.2 GKK21 pKa = 10.56 KK22 pKa = 10.4 FLDD25 pKa = 4.0 LPEE28 pKa = 6.2 KK29 pKa = 10.45 IVDD32 pKa = 4.77 AIDD35 pKa = 4.77 DD36 pKa = 4.05 YY37 pKa = 11.94 FDD39 pKa = 3.67 GQEE42 pKa = 3.8 IDD44 pKa = 3.6 FSGWANPDD52 pKa = 2.83 NVYY55 pKa = 10.82 INLYY59 pKa = 9.33 YY60 pKa = 10.42 VEE62 pKa = 4.34 SPKK65 pKa = 10.43 TVLVDD70 pKa = 3.9 IIGFMTDD77 pKa = 2.41 IEE79 pKa = 4.5 YY80 pKa = 10.86 EE81 pKa = 4.15 EE82 pKa = 5.39 HH83 pKa = 6.93 FLGNDD88 pKa = 3.31 GLEE91 pKa = 4.13 EE92 pKa = 4.78 FIEE95 pKa = 4.14 LWMEE99 pKa = 4.56 KK100 pKa = 9.71 ISEE103 pKa = 4.17 RR104 pKa = 11.84 LNEE107 pKa = 3.98 EE108 pKa = 4.07 VTFLGYY114 pKa = 10.87 ADD116 pKa = 4.32 GEE118 pKa = 4.4 FHH120 pKa = 7.72 FFQQ123 pKa = 5.73
Molecular weight: 14.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.872
IPC_protein 3.834
Toseland 3.63
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.101
Thurlkill 3.681
EMBOSS 3.732
Sillero 3.948
Patrickios 0.693
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.836
Protein with the highest isoelectric point:
>tr|A0A3P1VGS9|A0A3P1VGS9_9STRE Class A sortase OS=Streptococcus minor OX=229549 GN=EII38_01470 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.26 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 KK15 pKa = 8.35 HH16 pKa = 4.78 GFRR19 pKa = 11.84 NRR21 pKa = 11.84 MSTKK25 pKa = 9.22 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.78 GRR39 pKa = 11.84 KK40 pKa = 8.38 VLAVAA45 pKa = 4.94
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1898
0
1898
573303
26
1671
302.1
33.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.644 ± 0.062
0.507 ± 0.016
5.619 ± 0.047
6.936 ± 0.061
4.561 ± 0.05
6.65 ± 0.049
2.02 ± 0.028
7.361 ± 0.048
6.399 ± 0.048
10.188 ± 0.079
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.51 ± 0.028
4.245 ± 0.04
3.395 ± 0.029
4.282 ± 0.044
4.076 ± 0.04
6.114 ± 0.046
5.783 ± 0.063
6.995 ± 0.045
0.903 ± 0.02
3.813 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here