Nitrosospira sp. Nsp14

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosospira; unclassified Nitrosospira

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3081 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3AYG6|A0A1I3AYG6_9PROT N-acyl homoserine lactone hydrolase OS=Nitrosospira sp. Nsp14 OX=1855333 GN=SAMN05216299_12130 PE=4 SV=1
MM1 pKa = 7.33SSVYY5 pKa = 10.78SSGTYY10 pKa = 6.59TTVGPAPTGNSTINALIVGTKK31 pKa = 9.0WGASGLGGSANVTFSFPVSFTVGSSGAHH59 pKa = 4.87SSGAFGYY66 pKa = 8.9TGEE69 pKa = 4.64PQDD72 pKa = 4.11SGQALSAMQMAAARR86 pKa = 11.84EE87 pKa = 4.17TLHH90 pKa = 5.73KK91 pKa = 8.85WSRR94 pKa = 11.84VATLNCNEE102 pKa = 4.12VADD105 pKa = 4.76TIDD108 pKa = 3.86TKK110 pKa = 10.82WEE112 pKa = 4.05SGDD115 pKa = 3.52NSSVGDD121 pKa = 3.54IRR123 pKa = 11.84LAMSNLPSAAWAYY136 pKa = 11.15YY137 pKa = 9.15PGQDD141 pKa = 3.26NAPYY145 pKa = 10.74GGDD148 pKa = 2.89VWLNKK153 pKa = 9.75SDD155 pKa = 3.56YY156 pKa = 9.35HH157 pKa = 7.86DD158 pKa = 4.17PVVGYY163 pKa = 8.49YY164 pKa = 10.36AYY166 pKa = 9.65YY167 pKa = 10.25TFLHH171 pKa = 6.78EE172 pKa = 4.76IGHH175 pKa = 5.3TLGLEE180 pKa = 4.38HH181 pKa = 6.79PHH183 pKa = 6.68EE184 pKa = 5.22SNDD187 pKa = 3.41NDD189 pKa = 3.9VMSLSVDD196 pKa = 3.17ALKK199 pKa = 11.01YY200 pKa = 10.78SIMSYY205 pKa = 10.33RR206 pKa = 11.84DD207 pKa = 3.39YY208 pKa = 11.57VGDD211 pKa = 5.14DD212 pKa = 2.96IDD214 pKa = 4.63GVGEE218 pKa = 4.21NFFPTTPMLYY228 pKa = 10.3DD229 pKa = 3.56VLALQALYY237 pKa = 10.1GANWNYY243 pKa = 10.71RR244 pKa = 11.84SGNDD248 pKa = 3.37TYY250 pKa = 11.74SWAPGAKK257 pKa = 9.39VYY259 pKa = 8.0EE260 pKa = 4.59TIWDD264 pKa = 3.63GGGIDD269 pKa = 4.0TLSAANQTRR278 pKa = 11.84DD279 pKa = 3.43VEE281 pKa = 4.28LHH283 pKa = 5.34LTSGVFSKK291 pKa = 10.42IGSPVWDD298 pKa = 3.38EE299 pKa = 4.26HH300 pKa = 7.34AWVRR304 pKa = 11.84DD305 pKa = 3.47NLVIAFNATIEE316 pKa = 4.16NATGSSYY323 pKa = 11.54DD324 pKa = 4.01DD325 pKa = 3.96YY326 pKa = 11.67IFGNTAGNVLVGGPGNDD343 pKa = 3.17TMRR346 pKa = 11.84GSEE349 pKa = 3.79WADD352 pKa = 2.8RR353 pKa = 11.84DD354 pKa = 3.98VFDD357 pKa = 5.14NDD359 pKa = 3.19TMYY362 pKa = 11.05GGSGGDD368 pKa = 3.97LMAGHH373 pKa = 6.9NGNDD377 pKa = 3.06VMYY380 pKa = 10.12GGEE383 pKa = 4.21GNDD386 pKa = 3.71FVTGDD391 pKa = 3.82SGNDD395 pKa = 3.02ILSGGNGNDD404 pKa = 3.51ILNGGPGDD412 pKa = 4.1DD413 pKa = 4.64LLIGGLGNDD422 pKa = 3.96TLNGGGGFDD431 pKa = 3.54EE432 pKa = 4.51ATYY435 pKa = 10.8AAATAPVNVDD445 pKa = 3.72LSLSGGRR452 pKa = 11.84ATGAQGIDD460 pKa = 3.2KK461 pKa = 10.61LRR463 pKa = 11.84GIEE466 pKa = 4.22KK467 pKa = 8.7VTGSAFADD475 pKa = 3.96TLTGDD480 pKa = 3.36ANANVLSGRR489 pKa = 11.84LGNDD493 pKa = 3.29TLQGGAGNDD502 pKa = 3.55TLAGGGGDD510 pKa = 5.48DD511 pKa = 4.31LFRR514 pKa = 11.84WTATAFNSGDD524 pKa = 3.16IRR526 pKa = 11.84FGGVDD531 pKa = 3.12RR532 pKa = 11.84VSDD535 pKa = 4.33FSRR538 pKa = 11.84GDD540 pKa = 3.47ALDD543 pKa = 4.48FSAALEE549 pKa = 4.07SLLMVNGTRR558 pKa = 11.84LGAATSDD565 pKa = 3.22IALGNALSISTNVCLQNGRR584 pKa = 11.84DD585 pKa = 3.87LYY587 pKa = 10.84IDD589 pKa = 4.6LDD591 pKa = 3.97SSHH594 pKa = 7.21SITANDD600 pKa = 3.44YY601 pKa = 9.95HH602 pKa = 8.82VEE604 pKa = 4.01LTGLGGVLKK613 pKa = 10.58YY614 pKa = 10.59DD615 pKa = 3.52AAADD619 pKa = 3.94LFHH622 pKa = 6.76VV623 pKa = 4.49

Molecular weight: 65.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2ZTJ4|A0A1I2ZTJ4_9PROT ATP-dependent Clp protease ATP-binding subunit ClpA OS=Nitrosospira sp. Nsp14 OX=1855333 GN=SAMN05216299_11138 PE=3 SV=1
MM1 pKa = 7.9SPRR4 pKa = 11.84LTNNYY9 pKa = 7.63TLHH12 pKa = 6.21KK13 pKa = 7.15TTHH16 pKa = 4.32VWKK19 pKa = 10.53KK20 pKa = 9.05LQCQYY25 pKa = 10.91GCRR28 pKa = 11.84DD29 pKa = 3.52LRR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 9.78INARR37 pKa = 11.84GAIGVMAMVRR47 pKa = 11.84VEE49 pKa = 3.78RR50 pKa = 11.84STRR53 pKa = 11.84RR54 pKa = 11.84QEE56 pKa = 3.93ILDD59 pKa = 3.37YY60 pKa = 10.0WVRR63 pKa = 11.84ADD65 pKa = 3.5SVIIEE70 pKa = 3.96FRR72 pKa = 11.84PPGRR76 pKa = 11.84YY77 pKa = 9.2CSVFAAILILAA88 pKa = 4.82

Molecular weight: 10.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3081

0

3081

940644

25

4044

305.3

33.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.239 ± 0.054

0.967 ± 0.015

5.297 ± 0.036

5.977 ± 0.045

3.835 ± 0.035

8.007 ± 0.054

2.365 ± 0.021

5.583 ± 0.035

4.051 ± 0.04

10.42 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.468 ± 0.025

3.45 ± 0.034

4.881 ± 0.035

3.683 ± 0.027

6.518 ± 0.043

5.997 ± 0.033

5.149 ± 0.035

7.07 ± 0.038

1.323 ± 0.021

2.721 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski