Aeromonas phage 14AhydR10PP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1PFN8|A0A2S1PFN8_9CAUD Uncharacterized protein OS=Aeromonas phage 14AhydR10PP OX=2163987 PE=4 SV=1
MM1 pKa = 7.49SKK3 pKa = 10.22QVIAGVTIAQGEE15 pKa = 4.55VSCGVKK21 pKa = 10.39SPAYY25 pKa = 10.05DD26 pKa = 4.39PISPEE31 pKa = 3.7VPTFGEE37 pKa = 4.64YY38 pKa = 9.3WSHH41 pKa = 5.31TAMNGLPEE49 pKa = 4.22MTWDD53 pKa = 3.51AFQARR58 pKa = 11.84MMNQYY63 pKa = 10.91GLTILYY69 pKa = 10.27RR70 pKa = 11.84SMADD74 pKa = 3.4DD75 pKa = 5.28PEE77 pKa = 4.29VDD79 pKa = 3.5WEE81 pKa = 4.57DD82 pKa = 3.67EE83 pKa = 4.21LEE85 pKa = 4.28IEE87 pKa = 4.35SWEE90 pKa = 3.96PQNPFVSDD98 pKa = 3.4RR99 pKa = 11.84AFLLVLGEE107 pKa = 4.84DD108 pKa = 3.53EE109 pKa = 5.73DD110 pKa = 5.54GPFTVWGLPQQ120 pKa = 3.85

Molecular weight: 13.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1PFM8|A0A2S1PFM8_9CAUD Tail fiber protein OS=Aeromonas phage 14AhydR10PP OX=2163987 PE=4 SV=1
MM1 pKa = 6.97TRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84TPVIATSIRR14 pKa = 11.84HH15 pKa = 6.21PFTVHH20 pKa = 6.32HH21 pKa = 6.22FVSLKK26 pKa = 10.71DD27 pKa = 3.41AVEE30 pKa = 4.05RR31 pKa = 11.84GGFNQTSVSRR41 pKa = 11.84CLRR44 pKa = 11.84NPDD47 pKa = 3.39STHH50 pKa = 6.69CGFKK54 pKa = 10.0FTTTVTLEE62 pKa = 3.86PLRR65 pKa = 11.84ATKK68 pKa = 10.49RR69 pKa = 11.84RR70 pKa = 11.84DD71 pKa = 3.43EE72 pKa = 4.17VHH74 pKa = 6.6ALAAKK79 pKa = 10.06GYY81 pKa = 8.45SFSLIALEE89 pKa = 4.4LGIARR94 pKa = 11.84STVCYY99 pKa = 10.36HH100 pKa = 5.89MRR102 pKa = 11.84TPAA105 pKa = 3.4

Molecular weight: 11.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

14378

44

843

175.3

19.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.127 ± 0.413

1.537 ± 0.146

5.439 ± 0.224

6.267 ± 0.337

3.074 ± 0.137

7.769 ± 0.338

2.177 ± 0.185

4.952 ± 0.17

5.898 ± 0.296

8.43 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.234 ± 0.178

3.491 ± 0.302

4.41 ± 0.23

3.749 ± 0.189

6.051 ± 0.269

5.912 ± 0.203

6.287 ± 0.284

7.122 ± 0.311

1.586 ± 0.097

2.49 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski