Tunis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Nairoviridae; Orthonairovirus; Tunis orthonairovirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A191KWE5|A0A191KWE5_9VIRU Nucleoprotein OS=Tunis virus OX=1810944 PE=3 SV=1
MM1 pKa = 7.93ADD3 pKa = 3.36YY4 pKa = 9.25TYY6 pKa = 11.59KK7 pKa = 10.79LDD9 pKa = 4.11FRR11 pKa = 11.84SEE13 pKa = 4.4LEE15 pKa = 3.61WNAWYY20 pKa = 10.99NEE22 pKa = 3.89FKK24 pKa = 10.81GIHH27 pKa = 5.84PTLNSNLTNFDD38 pKa = 3.6SFMMEE43 pKa = 4.35LPNTRR48 pKa = 11.84EE49 pKa = 4.48FIDD52 pKa = 3.76DD53 pKa = 3.7MARR56 pKa = 11.84HH57 pKa = 5.99RR58 pKa = 11.84EE59 pKa = 3.85DD60 pKa = 3.82ARR62 pKa = 11.84ALKK65 pKa = 10.6DD66 pKa = 3.78SIFGKK71 pKa = 10.61AVIGLTAHH79 pKa = 6.59SAPVKK84 pKa = 9.43EE85 pKa = 4.71CAWLSNRR92 pKa = 11.84TFIKK96 pKa = 10.48HH97 pKa = 4.95ALEE100 pKa = 3.8WFEE103 pKa = 5.19DD104 pKa = 3.75NKK106 pKa = 10.47NKK108 pKa = 10.15QMVKK112 pKa = 10.26LWDD115 pKa = 3.39AEE117 pKa = 4.37YY118 pKa = 10.72NKK120 pKa = 10.73LMTTLPTLKK129 pKa = 9.96QVQSYY134 pKa = 10.23QDD136 pKa = 3.11AARR139 pKa = 11.84QFRR142 pKa = 11.84IDD144 pKa = 3.4VPLPKK149 pKa = 10.38DD150 pKa = 3.12QAFAVLEE157 pKa = 4.2GEE159 pKa = 4.36VLVEE163 pKa = 3.92YY164 pKa = 10.66KK165 pKa = 10.45VGRR168 pKa = 11.84DD169 pKa = 3.1ISKK172 pKa = 9.48TIMDD176 pKa = 4.54MLLDD180 pKa = 3.53MEE182 pKa = 4.6RR183 pKa = 11.84RR184 pKa = 11.84RR185 pKa = 11.84SEE187 pKa = 3.76TRR189 pKa = 11.84NPGAGGAEE197 pKa = 4.05NRR199 pKa = 11.84RR200 pKa = 11.84EE201 pKa = 4.08TLDD204 pKa = 4.82NNAAWMNSWLRR215 pKa = 11.84GRR217 pKa = 11.84HH218 pKa = 6.16GILDD222 pKa = 3.97MPPWGPWDD230 pKa = 3.53KK231 pKa = 10.78TSSNGYY237 pKa = 9.8LIAHH241 pKa = 6.14TSILKK246 pKa = 10.28AEE248 pKa = 4.11QNIEE252 pKa = 4.05EE253 pKa = 4.8DD254 pKa = 3.58LDD256 pKa = 4.15DD257 pKa = 4.53LVEE260 pKa = 4.02EE261 pKa = 4.47RR262 pKa = 11.84RR263 pKa = 11.84NDD265 pKa = 3.35VALLYY270 pKa = 10.29ARR272 pKa = 11.84PAAEE276 pKa = 4.88LADD279 pKa = 3.92FDD281 pKa = 3.81ITVIEE286 pKa = 4.82LIIKK290 pKa = 8.18TLEE293 pKa = 3.78TFQDD297 pKa = 3.91FKK299 pKa = 11.32RR300 pKa = 11.84RR301 pKa = 11.84IRR303 pKa = 11.84ARR305 pKa = 11.84TGQNVGGFGQQAAALDD321 pKa = 4.4TVFSSCYY328 pKa = 8.67WMWKK332 pKa = 10.04AGVTEE337 pKa = 4.26TSFPALSKK345 pKa = 10.35FLHH348 pKa = 5.98EE349 pKa = 5.37LGSKK353 pKa = 10.66AIGKK357 pKa = 7.26TKK359 pKa = 10.38LLSVLKK365 pKa = 10.38SCGWKK370 pKa = 8.61WGKK373 pKa = 10.31GLASIISAGDD383 pKa = 3.43FQGDD387 pKa = 4.14RR388 pKa = 11.84IHH390 pKa = 6.1MHH392 pKa = 6.44PAVLTSGRR400 pKa = 11.84LSMDD404 pKa = 3.64LVLSFGAVPTFNPDD418 pKa = 2.93LAMEE422 pKa = 4.42PVGSLRR428 pKa = 11.84SLLNMEE434 pKa = 4.4TNRR437 pKa = 11.84GNTCAKK443 pKa = 10.41AIVQLWDD450 pKa = 3.34VFNAGYY456 pKa = 10.1RR457 pKa = 11.84YY458 pKa = 9.73EE459 pKa = 4.59EE460 pKa = 4.35EE461 pKa = 5.86DD462 pKa = 3.47IVPPEE467 pKa = 4.13HH468 pKa = 6.67MLHH471 pKa = 6.22QSFLGKK477 pKa = 10.09ISPFQNVSLRR487 pKa = 11.84RR488 pKa = 11.84GDD490 pKa = 3.57ALKK493 pKa = 11.1VKK495 pKa = 10.4IITT498 pKa = 3.81

Molecular weight:
56.61 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A191KWE5|A0A191KWE5_9VIRU Nucleoprotein OS=Tunis virus OX=1810944 PE=3 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84FILLYY7 pKa = 10.02YY8 pKa = 10.53SLGILTAGYY17 pKa = 9.25LVCCTNNTTTTNNATSVTPQIGLNSTVPTNSSTTGNLTSRR57 pKa = 11.84HH58 pKa = 5.26PVIDD62 pKa = 4.74GIVKK66 pKa = 10.12AFSFFSSGIDD76 pKa = 3.35RR77 pKa = 11.84TDD79 pKa = 3.04LAANLLSSYY88 pKa = 11.14YY89 pKa = 7.71YY90 pKa = 9.37TQYY93 pKa = 10.62FRR95 pKa = 11.84NKK97 pKa = 9.34LKK99 pKa = 10.59QSYY102 pKa = 8.52TSLSSAVLDD111 pKa = 3.68AFKK114 pKa = 10.97VIVVGPYY121 pKa = 9.41RR122 pKa = 11.84GEE124 pKa = 3.85NRR126 pKa = 11.84TAALQPTPPSSLEE139 pKa = 3.91KK140 pKa = 10.32LVSMVNDD147 pKa = 5.04RR148 pKa = 11.84ILTGYY153 pKa = 10.59EE154 pKa = 3.44EE155 pKa = 4.28VEE157 pKa = 4.03YY158 pKa = 11.11KK159 pKa = 10.48FFKK162 pKa = 10.19KK163 pKa = 10.54DD164 pKa = 3.16SVADD168 pKa = 3.62RR169 pKa = 11.84GYY171 pKa = 9.3SVRR174 pKa = 11.84VRR176 pKa = 11.84FEE178 pKa = 4.24LNQTGTKK185 pKa = 9.98SYY187 pKa = 9.63TRR189 pKa = 11.84QPVDD193 pKa = 3.06EE194 pKa = 5.76LGYY197 pKa = 10.64LWSEE201 pKa = 4.36DD202 pKa = 3.25GGVSMVCLEE211 pKa = 3.83GRR213 pKa = 11.84WYY215 pKa = 10.17DD216 pKa = 3.74SKK218 pKa = 11.64ADD220 pKa = 3.75SILRR224 pKa = 11.84TKK226 pKa = 9.89ATGSSIPGDD235 pKa = 3.47CSTFQPTPLKK245 pKa = 10.58SLSYY249 pKa = 11.07QNLFLSKK256 pKa = 10.62DD257 pKa = 3.09AGHH260 pKa = 6.73NLRR263 pKa = 11.84VVNYY267 pKa = 7.29HH268 pKa = 4.64TTQFGFSFLNCEE280 pKa = 4.29VVLEE284 pKa = 4.29SAGCVMRR291 pKa = 11.84TVKK294 pKa = 10.59LPEE297 pKa = 4.34WINLNFHH304 pKa = 7.01NDD306 pKa = 2.77THH308 pKa = 6.75QYY310 pKa = 9.14PLLHH314 pKa = 7.06ILINEE319 pKa = 4.03NLDD322 pKa = 3.38NTGCKK327 pKa = 10.17LRR329 pKa = 11.84MCAVSNRR336 pKa = 11.84AGKK339 pKa = 10.25SLGEE343 pKa = 3.86MRR345 pKa = 11.84FKK347 pKa = 10.86GVEE350 pKa = 4.04VLVDD354 pKa = 3.75RR355 pKa = 11.84PISGTGRR362 pKa = 11.84KK363 pKa = 9.22LLDD366 pKa = 3.04VRR368 pKa = 11.84EE369 pKa = 4.19PVTRR373 pKa = 11.84GYY375 pKa = 11.12KK376 pKa = 9.53NRR378 pKa = 11.84NICNTKK384 pKa = 10.06NQLLPYY390 pKa = 9.84QKK392 pKa = 10.99SLMHH396 pKa = 6.33NLHH399 pKa = 6.61RR400 pKa = 11.84SVPGTKK406 pKa = 9.62VSYY409 pKa = 10.94CNGSVHH415 pKa = 5.37TTLPLGSLHH424 pKa = 6.38GCYY427 pKa = 10.01SVGTVTTHH435 pKa = 5.8HH436 pKa = 6.05QCPGLKK442 pKa = 9.73RR443 pKa = 11.84GLSGKK448 pKa = 9.35MSTIEE453 pKa = 4.2NVNCSIDD460 pKa = 3.65YY461 pKa = 10.23HH462 pKa = 8.33IKK464 pKa = 9.2EE465 pKa = 4.84CSNGHH470 pKa = 5.48YY471 pKa = 10.05CFQVTMLGSGAVTVKK486 pKa = 10.51SNHH489 pKa = 4.7HH490 pKa = 6.41HH491 pKa = 5.6EE492 pKa = 4.52VVNCKK497 pKa = 10.13HH498 pKa = 6.08EE499 pKa = 4.67CLLSLPDD506 pKa = 3.79TQEE509 pKa = 4.02DD510 pKa = 4.8TIVTCPDD517 pKa = 3.41GKK519 pKa = 9.11SHH521 pKa = 7.39RR522 pKa = 11.84LLYY525 pKa = 10.67NLLIHH530 pKa = 7.14DD531 pKa = 4.83CPFEE535 pKa = 5.55RR536 pKa = 11.84YY537 pKa = 9.08LGHH540 pKa = 6.71NAFFICRR547 pKa = 11.84MSHH550 pKa = 6.11HH551 pKa = 6.9PRR553 pKa = 11.84LVYY556 pKa = 10.68CLFFWIFGGCPLLYY570 pKa = 10.04VTFTLIKK577 pKa = 10.34FSILALAWTVIKK589 pKa = 10.57VRR591 pKa = 11.84RR592 pKa = 11.84TLTRR596 pKa = 11.84EE597 pKa = 3.61KK598 pKa = 10.01GTCDD602 pKa = 2.92QCGDD606 pKa = 4.15FVPTGPEE613 pKa = 3.74WQRR616 pKa = 11.84HH617 pKa = 4.52DD618 pKa = 3.32QCKK621 pKa = 10.05QGSCPYY627 pKa = 9.39CRR629 pKa = 11.84SRR631 pKa = 11.84FSISGLKK638 pKa = 8.47QHH640 pKa = 6.54IQVCLEE646 pKa = 3.7RR647 pKa = 11.84DD648 pKa = 3.23RR649 pKa = 11.84VRR651 pKa = 11.84NSDD654 pKa = 3.83EE655 pKa = 3.7EE656 pKa = 4.49VMNRR660 pKa = 11.84KK661 pKa = 8.64LVCYY665 pKa = 8.91PISIAGRR672 pKa = 11.84LASKK676 pKa = 9.78AQKK679 pKa = 8.78HH680 pKa = 4.26TVKK683 pKa = 10.74VGWFIILLILLLLSTRR699 pKa = 11.84PANGLQNVEE708 pKa = 4.06LQKK711 pKa = 11.05GIWEE715 pKa = 4.2QEE717 pKa = 3.97VEE719 pKa = 4.23EE720 pKa = 4.64VEE722 pKa = 4.48VCKK725 pKa = 10.66VDD727 pKa = 3.7CTVEE731 pKa = 4.6SSLCYY736 pKa = 10.37CEE738 pKa = 5.28MEE740 pKa = 4.2QLQDD744 pKa = 3.2FHH746 pKa = 8.27SGRR749 pKa = 11.84RR750 pKa = 11.84LLFNHH755 pKa = 7.1PAMSGIYY762 pKa = 9.8SKK764 pKa = 11.18NPNVTRR770 pKa = 11.84RR771 pKa = 11.84TVEE774 pKa = 3.79VINNVKK780 pKa = 10.5APWGIVNVKK789 pKa = 8.64STHH792 pKa = 5.02QPTYY796 pKa = 10.65SSSALQMSWSSSDD809 pKa = 3.4EE810 pKa = 4.32LEE812 pKa = 4.91DD813 pKa = 4.25GSIVLSGRR821 pKa = 11.84SSSILKK827 pKa = 10.22LEE829 pKa = 4.68PNTGVTWNIKK839 pKa = 9.52AKK841 pKa = 9.44QSKK844 pKa = 7.9EE845 pKa = 3.72EE846 pKa = 4.09RR847 pKa = 11.84KK848 pKa = 10.38VSISVIDD855 pKa = 3.58HH856 pKa = 4.92SQIYY860 pKa = 8.79NARR863 pKa = 11.84FQYY866 pKa = 9.6LTGDD870 pKa = 3.68RR871 pKa = 11.84TVGSWMHH878 pKa = 5.47GTCTEE883 pKa = 4.05VCPEE887 pKa = 3.93KK888 pKa = 10.71CGCTMSSCHH897 pKa = 5.95HH898 pKa = 6.32LEE900 pKa = 3.83WLKK903 pKa = 11.07SRR905 pKa = 11.84NWHH908 pKa = 6.85CNPLWCWAMDD918 pKa = 4.06QGCTCCAIDD927 pKa = 4.32IQNVYY932 pKa = 10.13NNWVVSKK939 pKa = 8.36WTLEE943 pKa = 4.23YY944 pKa = 10.37IGTEE948 pKa = 3.72ALVCVDD954 pKa = 3.44YY955 pKa = 11.5DD956 pKa = 3.57NSEE959 pKa = 4.21RR960 pKa = 11.84EE961 pKa = 3.89CDD963 pKa = 3.35IVNTATQFEE972 pKa = 4.58SGPYY976 pKa = 8.62QFQVSDD982 pKa = 3.56VSNVQHH988 pKa = 6.98RR989 pKa = 11.84LPSEE993 pKa = 3.36ILIFHH998 pKa = 7.6KK999 pKa = 10.37IPKK1002 pKa = 10.03DD1003 pKa = 3.44ADD1005 pKa = 3.55TLDD1008 pKa = 3.67LMKK1011 pKa = 10.29HH1012 pKa = 4.22YY1013 pKa = 10.41HH1014 pKa = 5.66ITSAEE1019 pKa = 4.12NACKK1023 pKa = 10.21LQSCTHH1029 pKa = 6.25GSVGDD1034 pKa = 3.87SQIYY1038 pKa = 10.66DD1039 pKa = 3.93LDD1041 pKa = 4.01RR1042 pKa = 11.84LIGNDD1047 pKa = 2.6IDD1049 pKa = 4.63AEE1051 pKa = 4.38HH1052 pKa = 6.59FFKK1055 pKa = 11.03NVSGKK1060 pKa = 9.61EE1061 pKa = 3.76RR1062 pKa = 11.84GHH1064 pKa = 4.89WMSWEE1069 pKa = 4.09GVTMDD1074 pKa = 3.27YY1075 pKa = 10.92HH1076 pKa = 7.24CNQGHH1081 pKa = 6.36WPDD1084 pKa = 3.54CVYY1087 pKa = 10.83TGVVEE1092 pKa = 4.32QNSEE1096 pKa = 3.81AFANLLSVEE1105 pKa = 3.83QDD1107 pKa = 3.23YY1108 pKa = 9.36TKK1110 pKa = 10.71TFFFHH1115 pKa = 7.03NVNCRR1120 pKa = 11.84INGSVPALDD1129 pKa = 3.39LQARR1133 pKa = 11.84SKK1135 pKa = 10.9VSPGSLSVFLEE1146 pKa = 4.44VKK1148 pKa = 10.4GLTLMSDD1155 pKa = 3.53KK1156 pKa = 11.41AHH1158 pKa = 6.03LTSLSFRR1165 pKa = 11.84TLSCTGCYY1173 pKa = 10.37GCVEE1177 pKa = 4.59GGTCSVIVAMSGVPKK1192 pKa = 10.38IGLHH1196 pKa = 4.66FVSMTEE1202 pKa = 3.93HH1203 pKa = 5.16VTVEE1207 pKa = 3.86QSTEE1211 pKa = 4.1LIEE1214 pKa = 4.61EE1215 pKa = 4.33NEE1217 pKa = 4.03PTTTNIRR1224 pKa = 11.84FFSAAPVKK1232 pKa = 9.7EE1233 pKa = 3.92LCIKK1237 pKa = 10.64LKK1239 pKa = 10.78EE1240 pKa = 3.97FTLCKK1245 pKa = 9.99TCDD1248 pKa = 3.15KK1249 pKa = 10.45AKK1251 pKa = 10.28QVSCVKK1257 pKa = 10.51VSLKK1261 pKa = 10.85DD1262 pKa = 3.42PEE1264 pKa = 4.45RR1265 pKa = 11.84VLLEE1269 pKa = 3.76HH1270 pKa = 7.14RR1271 pKa = 11.84GTLVSTTTDD1280 pKa = 2.71NCTSIVSCWAQSFTGFGLGIGNLLSYY1306 pKa = 10.92LGGSLLKK1313 pKa = 10.92GIILFVLPLATVVLLILYY1331 pKa = 7.99GPSVVRR1337 pKa = 11.84LLVKK1341 pKa = 10.04FRR1343 pKa = 11.84RR1344 pKa = 11.84LRR1346 pKa = 11.84GLARR1350 pKa = 11.84KK1351 pKa = 9.26ARR1353 pKa = 11.84ARR1355 pKa = 11.84RR1356 pKa = 11.84FDD1358 pKa = 3.24NDD1360 pKa = 3.69GIMEE1364 pKa = 4.35IRR1366 pKa = 11.84QALLKK1371 pKa = 10.83KK1372 pKa = 9.9EE1373 pKa = 4.93DD1374 pKa = 3.99KK1375 pKa = 10.88DD1376 pKa = 3.6EE1377 pKa = 4.91HH1378 pKa = 8.34DD1379 pKa = 3.58LMNYY1383 pKa = 8.53FVKK1386 pKa = 10.94NKK1388 pKa = 10.18

Molecular weight:
155.97 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

5841

498

3955

1947.0

220.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.691 ± 0.711

3.065 ± 0.564

5.324 ± 0.415

6.403 ± 0.483

4.16 ± 0.168

4.674 ± 1.015

2.431 ± 0.464

5.855 ± 0.542

6.917 ± 0.687

10.871 ± 0.439

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.209 ± 0.241

5.068 ± 0.08

3.27 ± 0.161

3.647 ± 0.177

4.794 ± 0.328

8.851 ± 0.571

6.489 ± 0.281

7.105 ± 0.486

1.318 ± 0.364

2.859 ± 0.243

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski