Tunis virus
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191KWE5|A0A191KWE5_9VIRU Nucleoprotein OS=Tunis virus OX=1810944 PE=3 SV=1
MM1 pKa = 7.93 ADD3 pKa = 3.36 YY4 pKa = 9.25 TYY6 pKa = 11.59 KK7 pKa = 10.79 LDD9 pKa = 4.11 FRR11 pKa = 11.84 SEE13 pKa = 4.4 LEE15 pKa = 3.61 WNAWYY20 pKa = 10.99 NEE22 pKa = 3.89 FKK24 pKa = 10.81 GIHH27 pKa = 5.84 PTLNSNLTNFDD38 pKa = 3.6 SFMMEE43 pKa = 4.35 LPNTRR48 pKa = 11.84 EE49 pKa = 4.48 FIDD52 pKa = 3.76 DD53 pKa = 3.7 MARR56 pKa = 11.84 HH57 pKa = 5.99 RR58 pKa = 11.84 EE59 pKa = 3.85 DD60 pKa = 3.82 ARR62 pKa = 11.84 ALKK65 pKa = 10.6 DD66 pKa = 3.78 SIFGKK71 pKa = 10.61 AVIGLTAHH79 pKa = 6.59 SAPVKK84 pKa = 9.43 EE85 pKa = 4.71 CAWLSNRR92 pKa = 11.84 TFIKK96 pKa = 10.48 HH97 pKa = 4.95 ALEE100 pKa = 3.8 WFEE103 pKa = 5.19 DD104 pKa = 3.75 NKK106 pKa = 10.47 NKK108 pKa = 10.15 QMVKK112 pKa = 10.26 LWDD115 pKa = 3.39 AEE117 pKa = 4.37 YY118 pKa = 10.72 NKK120 pKa = 10.73 LMTTLPTLKK129 pKa = 9.96 QVQSYY134 pKa = 10.23 QDD136 pKa = 3.11 AARR139 pKa = 11.84 QFRR142 pKa = 11.84 IDD144 pKa = 3.4 VPLPKK149 pKa = 10.38 DD150 pKa = 3.12 QAFAVLEE157 pKa = 4.2 GEE159 pKa = 4.36 VLVEE163 pKa = 3.92 YY164 pKa = 10.66 KK165 pKa = 10.45 VGRR168 pKa = 11.84 DD169 pKa = 3.1 ISKK172 pKa = 9.48 TIMDD176 pKa = 4.54 MLLDD180 pKa = 3.53 MEE182 pKa = 4.6 RR183 pKa = 11.84 RR184 pKa = 11.84 RR185 pKa = 11.84 SEE187 pKa = 3.76 TRR189 pKa = 11.84 NPGAGGAEE197 pKa = 4.05 NRR199 pKa = 11.84 RR200 pKa = 11.84 EE201 pKa = 4.08 TLDD204 pKa = 4.82 NNAAWMNSWLRR215 pKa = 11.84 GRR217 pKa = 11.84 HH218 pKa = 6.16 GILDD222 pKa = 3.97 MPPWGPWDD230 pKa = 3.53 KK231 pKa = 10.78 TSSNGYY237 pKa = 9.8 LIAHH241 pKa = 6.14 TSILKK246 pKa = 10.28 AEE248 pKa = 4.11 QNIEE252 pKa = 4.05 EE253 pKa = 4.8 DD254 pKa = 3.58 LDD256 pKa = 4.15 DD257 pKa = 4.53 LVEE260 pKa = 4.02 EE261 pKa = 4.47 RR262 pKa = 11.84 RR263 pKa = 11.84 NDD265 pKa = 3.35 VALLYY270 pKa = 10.29 ARR272 pKa = 11.84 PAAEE276 pKa = 4.88 LADD279 pKa = 3.92 FDD281 pKa = 3.81 ITVIEE286 pKa = 4.82 LIIKK290 pKa = 8.18 TLEE293 pKa = 3.78 TFQDD297 pKa = 3.91 FKK299 pKa = 11.32 RR300 pKa = 11.84 RR301 pKa = 11.84 IRR303 pKa = 11.84 ARR305 pKa = 11.84 TGQNVGGFGQQAAALDD321 pKa = 4.4 TVFSSCYY328 pKa = 8.67 WMWKK332 pKa = 10.04 AGVTEE337 pKa = 4.26 TSFPALSKK345 pKa = 10.35 FLHH348 pKa = 5.98 EE349 pKa = 5.37 LGSKK353 pKa = 10.66 AIGKK357 pKa = 7.26 TKK359 pKa = 10.38 LLSVLKK365 pKa = 10.38 SCGWKK370 pKa = 8.61 WGKK373 pKa = 10.31 GLASIISAGDD383 pKa = 3.43 FQGDD387 pKa = 4.14 RR388 pKa = 11.84 IHH390 pKa = 6.1 MHH392 pKa = 6.44 PAVLTSGRR400 pKa = 11.84 LSMDD404 pKa = 3.64 LVLSFGAVPTFNPDD418 pKa = 2.93 LAMEE422 pKa = 4.42 PVGSLRR428 pKa = 11.84 SLLNMEE434 pKa = 4.4 TNRR437 pKa = 11.84 GNTCAKK443 pKa = 10.41 AIVQLWDD450 pKa = 3.34 VFNAGYY456 pKa = 10.1 RR457 pKa = 11.84 YY458 pKa = 9.73 EE459 pKa = 4.59 EE460 pKa = 4.35 EE461 pKa = 5.86 DD462 pKa = 3.47 IVPPEE467 pKa = 4.13 HH468 pKa = 6.67 MLHH471 pKa = 6.22 QSFLGKK477 pKa = 10.09 ISPFQNVSLRR487 pKa = 11.84 RR488 pKa = 11.84 GDD490 pKa = 3.57 ALKK493 pKa = 11.1 VKK495 pKa = 10.4 IITT498 pKa = 3.81
Molecular weight: 56.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.96
IPC2_protein 5.855
IPC_protein 5.919
Toseland 6.326
ProMoST 6.249
Dawson 6.173
Bjellqvist 6.173
Wikipedia 6.186
Rodwell 6.16
Grimsley 6.491
Solomon 6.173
Lehninger 6.16
Nozaki 6.427
DTASelect 6.62
Thurlkill 6.634
EMBOSS 6.59
Sillero 6.532
Patrickios 4.571
IPC_peptide 6.186
IPC2_peptide 6.478
IPC2.peptide.svr19 6.527
Protein with the highest isoelectric point:
>tr|A0A191KWE5|A0A191KWE5_9VIRU Nucleoprotein OS=Tunis virus OX=1810944 PE=3 SV=1
MM1 pKa = 7.82 RR2 pKa = 11.84 FILLYY7 pKa = 10.02 YY8 pKa = 10.53 SLGILTAGYY17 pKa = 9.25 LVCCTNNTTTTNNATSVTPQIGLNSTVPTNSSTTGNLTSRR57 pKa = 11.84 HH58 pKa = 5.26 PVIDD62 pKa = 4.74 GIVKK66 pKa = 10.12 AFSFFSSGIDD76 pKa = 3.35 RR77 pKa = 11.84 TDD79 pKa = 3.04 LAANLLSSYY88 pKa = 11.14 YY89 pKa = 7.71 YY90 pKa = 9.37 TQYY93 pKa = 10.62 FRR95 pKa = 11.84 NKK97 pKa = 9.34 LKK99 pKa = 10.59 QSYY102 pKa = 8.52 TSLSSAVLDD111 pKa = 3.68 AFKK114 pKa = 10.97 VIVVGPYY121 pKa = 9.41 RR122 pKa = 11.84 GEE124 pKa = 3.85 NRR126 pKa = 11.84 TAALQPTPPSSLEE139 pKa = 3.91 KK140 pKa = 10.32 LVSMVNDD147 pKa = 5.04 RR148 pKa = 11.84 ILTGYY153 pKa = 10.59 EE154 pKa = 3.44 EE155 pKa = 4.28 VEE157 pKa = 4.03 YY158 pKa = 11.11 KK159 pKa = 10.48 FFKK162 pKa = 10.19 KK163 pKa = 10.54 DD164 pKa = 3.16 SVADD168 pKa = 3.62 RR169 pKa = 11.84 GYY171 pKa = 9.3 SVRR174 pKa = 11.84 VRR176 pKa = 11.84 FEE178 pKa = 4.24 LNQTGTKK185 pKa = 9.98 SYY187 pKa = 9.63 TRR189 pKa = 11.84 QPVDD193 pKa = 3.06 EE194 pKa = 5.76 LGYY197 pKa = 10.64 LWSEE201 pKa = 4.36 DD202 pKa = 3.25 GGVSMVCLEE211 pKa = 3.83 GRR213 pKa = 11.84 WYY215 pKa = 10.17 DD216 pKa = 3.74 SKK218 pKa = 11.64 ADD220 pKa = 3.75 SILRR224 pKa = 11.84 TKK226 pKa = 9.89 ATGSSIPGDD235 pKa = 3.47 CSTFQPTPLKK245 pKa = 10.58 SLSYY249 pKa = 11.07 QNLFLSKK256 pKa = 10.62 DD257 pKa = 3.09 AGHH260 pKa = 6.73 NLRR263 pKa = 11.84 VVNYY267 pKa = 7.29 HH268 pKa = 4.64 TTQFGFSFLNCEE280 pKa = 4.29 VVLEE284 pKa = 4.29 SAGCVMRR291 pKa = 11.84 TVKK294 pKa = 10.59 LPEE297 pKa = 4.34 WINLNFHH304 pKa = 7.01 NDD306 pKa = 2.77 THH308 pKa = 6.75 QYY310 pKa = 9.14 PLLHH314 pKa = 7.06 ILINEE319 pKa = 4.03 NLDD322 pKa = 3.38 NTGCKK327 pKa = 10.17 LRR329 pKa = 11.84 MCAVSNRR336 pKa = 11.84 AGKK339 pKa = 10.25 SLGEE343 pKa = 3.86 MRR345 pKa = 11.84 FKK347 pKa = 10.86 GVEE350 pKa = 4.04 VLVDD354 pKa = 3.75 RR355 pKa = 11.84 PISGTGRR362 pKa = 11.84 KK363 pKa = 9.22 LLDD366 pKa = 3.04 VRR368 pKa = 11.84 EE369 pKa = 4.19 PVTRR373 pKa = 11.84 GYY375 pKa = 11.12 KK376 pKa = 9.53 NRR378 pKa = 11.84 NICNTKK384 pKa = 10.06 NQLLPYY390 pKa = 9.84 QKK392 pKa = 10.99 SLMHH396 pKa = 6.33 NLHH399 pKa = 6.61 RR400 pKa = 11.84 SVPGTKK406 pKa = 9.62 VSYY409 pKa = 10.94 CNGSVHH415 pKa = 5.37 TTLPLGSLHH424 pKa = 6.38 GCYY427 pKa = 10.01 SVGTVTTHH435 pKa = 5.8 HH436 pKa = 6.05 QCPGLKK442 pKa = 9.73 RR443 pKa = 11.84 GLSGKK448 pKa = 9.35 MSTIEE453 pKa = 4.2 NVNCSIDD460 pKa = 3.65 YY461 pKa = 10.23 HH462 pKa = 8.33 IKK464 pKa = 9.2 EE465 pKa = 4.84 CSNGHH470 pKa = 5.48 YY471 pKa = 10.05 CFQVTMLGSGAVTVKK486 pKa = 10.51 SNHH489 pKa = 4.7 HH490 pKa = 6.41 HH491 pKa = 5.6 EE492 pKa = 4.52 VVNCKK497 pKa = 10.13 HH498 pKa = 6.08 EE499 pKa = 4.67 CLLSLPDD506 pKa = 3.79 TQEE509 pKa = 4.02 DD510 pKa = 4.8 TIVTCPDD517 pKa = 3.41 GKK519 pKa = 9.11 SHH521 pKa = 7.39 RR522 pKa = 11.84 LLYY525 pKa = 10.67 NLLIHH530 pKa = 7.14 DD531 pKa = 4.83 CPFEE535 pKa = 5.55 RR536 pKa = 11.84 YY537 pKa = 9.08 LGHH540 pKa = 6.71 NAFFICRR547 pKa = 11.84 MSHH550 pKa = 6.11 HH551 pKa = 6.9 PRR553 pKa = 11.84 LVYY556 pKa = 10.68 CLFFWIFGGCPLLYY570 pKa = 10.04 VTFTLIKK577 pKa = 10.34 FSILALAWTVIKK589 pKa = 10.57 VRR591 pKa = 11.84 RR592 pKa = 11.84 TLTRR596 pKa = 11.84 EE597 pKa = 3.61 KK598 pKa = 10.01 GTCDD602 pKa = 2.92 QCGDD606 pKa = 4.15 FVPTGPEE613 pKa = 3.74 WQRR616 pKa = 11.84 HH617 pKa = 4.52 DD618 pKa = 3.32 QCKK621 pKa = 10.05 QGSCPYY627 pKa = 9.39 CRR629 pKa = 11.84 SRR631 pKa = 11.84 FSISGLKK638 pKa = 8.47 QHH640 pKa = 6.54 IQVCLEE646 pKa = 3.7 RR647 pKa = 11.84 DD648 pKa = 3.23 RR649 pKa = 11.84 VRR651 pKa = 11.84 NSDD654 pKa = 3.83 EE655 pKa = 3.7 EE656 pKa = 4.49 VMNRR660 pKa = 11.84 KK661 pKa = 8.64 LVCYY665 pKa = 8.91 PISIAGRR672 pKa = 11.84 LASKK676 pKa = 9.78 AQKK679 pKa = 8.78 HH680 pKa = 4.26 TVKK683 pKa = 10.74 VGWFIILLILLLLSTRR699 pKa = 11.84 PANGLQNVEE708 pKa = 4.06 LQKK711 pKa = 11.05 GIWEE715 pKa = 4.2 QEE717 pKa = 3.97 VEE719 pKa = 4.23 EE720 pKa = 4.64 VEE722 pKa = 4.48 VCKK725 pKa = 10.66 VDD727 pKa = 3.7 CTVEE731 pKa = 4.6 SSLCYY736 pKa = 10.37 CEE738 pKa = 5.28 MEE740 pKa = 4.2 QLQDD744 pKa = 3.2 FHH746 pKa = 8.27 SGRR749 pKa = 11.84 RR750 pKa = 11.84 LLFNHH755 pKa = 7.1 PAMSGIYY762 pKa = 9.8 SKK764 pKa = 11.18 NPNVTRR770 pKa = 11.84 RR771 pKa = 11.84 TVEE774 pKa = 3.79 VINNVKK780 pKa = 10.5 APWGIVNVKK789 pKa = 8.64 STHH792 pKa = 5.02 QPTYY796 pKa = 10.65 SSSALQMSWSSSDD809 pKa = 3.4 EE810 pKa = 4.32 LEE812 pKa = 4.91 DD813 pKa = 4.25 GSIVLSGRR821 pKa = 11.84 SSSILKK827 pKa = 10.22 LEE829 pKa = 4.68 PNTGVTWNIKK839 pKa = 9.52 AKK841 pKa = 9.44 QSKK844 pKa = 7.9 EE845 pKa = 3.72 EE846 pKa = 4.09 RR847 pKa = 11.84 KK848 pKa = 10.38 VSISVIDD855 pKa = 3.58 HH856 pKa = 4.92 SQIYY860 pKa = 8.79 NARR863 pKa = 11.84 FQYY866 pKa = 9.6 LTGDD870 pKa = 3.68 RR871 pKa = 11.84 TVGSWMHH878 pKa = 5.47 GTCTEE883 pKa = 4.05 VCPEE887 pKa = 3.93 KK888 pKa = 10.71 CGCTMSSCHH897 pKa = 5.95 HH898 pKa = 6.32 LEE900 pKa = 3.83 WLKK903 pKa = 11.07 SRR905 pKa = 11.84 NWHH908 pKa = 6.85 CNPLWCWAMDD918 pKa = 4.06 QGCTCCAIDD927 pKa = 4.32 IQNVYY932 pKa = 10.13 NNWVVSKK939 pKa = 8.36 WTLEE943 pKa = 4.23 YY944 pKa = 10.37 IGTEE948 pKa = 3.72 ALVCVDD954 pKa = 3.44 YY955 pKa = 11.5 DD956 pKa = 3.57 NSEE959 pKa = 4.21 RR960 pKa = 11.84 EE961 pKa = 3.89 CDD963 pKa = 3.35 IVNTATQFEE972 pKa = 4.58 SGPYY976 pKa = 8.62 QFQVSDD982 pKa = 3.56 VSNVQHH988 pKa = 6.98 RR989 pKa = 11.84 LPSEE993 pKa = 3.36 ILIFHH998 pKa = 7.6 KK999 pKa = 10.37 IPKK1002 pKa = 10.03 DD1003 pKa = 3.44 ADD1005 pKa = 3.55 TLDD1008 pKa = 3.67 LMKK1011 pKa = 10.29 HH1012 pKa = 4.22 YY1013 pKa = 10.41 HH1014 pKa = 5.66 ITSAEE1019 pKa = 4.12 NACKK1023 pKa = 10.21 LQSCTHH1029 pKa = 6.25 GSVGDD1034 pKa = 3.87 SQIYY1038 pKa = 10.66 DD1039 pKa = 3.93 LDD1041 pKa = 4.01 RR1042 pKa = 11.84 LIGNDD1047 pKa = 2.6 IDD1049 pKa = 4.63 AEE1051 pKa = 4.38 HH1052 pKa = 6.59 FFKK1055 pKa = 11.03 NVSGKK1060 pKa = 9.61 EE1061 pKa = 3.76 RR1062 pKa = 11.84 GHH1064 pKa = 4.89 WMSWEE1069 pKa = 4.09 GVTMDD1074 pKa = 3.27 YY1075 pKa = 10.92 HH1076 pKa = 7.24 CNQGHH1081 pKa = 6.36 WPDD1084 pKa = 3.54 CVYY1087 pKa = 10.83 TGVVEE1092 pKa = 4.32 QNSEE1096 pKa = 3.81 AFANLLSVEE1105 pKa = 3.83 QDD1107 pKa = 3.23 YY1108 pKa = 9.36 TKK1110 pKa = 10.71 TFFFHH1115 pKa = 7.03 NVNCRR1120 pKa = 11.84 INGSVPALDD1129 pKa = 3.39 LQARR1133 pKa = 11.84 SKK1135 pKa = 10.9 VSPGSLSVFLEE1146 pKa = 4.44 VKK1148 pKa = 10.4 GLTLMSDD1155 pKa = 3.53 KK1156 pKa = 11.41 AHH1158 pKa = 6.03 LTSLSFRR1165 pKa = 11.84 TLSCTGCYY1173 pKa = 10.37 GCVEE1177 pKa = 4.59 GGTCSVIVAMSGVPKK1192 pKa = 10.38 IGLHH1196 pKa = 4.66 FVSMTEE1202 pKa = 3.93 HH1203 pKa = 5.16 VTVEE1207 pKa = 3.86 QSTEE1211 pKa = 4.1 LIEE1214 pKa = 4.61 EE1215 pKa = 4.33 NEE1217 pKa = 4.03 PTTTNIRR1224 pKa = 11.84 FFSAAPVKK1232 pKa = 9.7 EE1233 pKa = 3.92 LCIKK1237 pKa = 10.64 LKK1239 pKa = 10.78 EE1240 pKa = 3.97 FTLCKK1245 pKa = 9.99 TCDD1248 pKa = 3.15 KK1249 pKa = 10.45 AKK1251 pKa = 10.28 QVSCVKK1257 pKa = 10.51 VSLKK1261 pKa = 10.85 DD1262 pKa = 3.42 PEE1264 pKa = 4.45 RR1265 pKa = 11.84 VLLEE1269 pKa = 3.76 HH1270 pKa = 7.14 RR1271 pKa = 11.84 GTLVSTTTDD1280 pKa = 2.71 NCTSIVSCWAQSFTGFGLGIGNLLSYY1306 pKa = 10.92 LGGSLLKK1313 pKa = 10.92 GIILFVLPLATVVLLILYY1331 pKa = 7.99 GPSVVRR1337 pKa = 11.84 LLVKK1341 pKa = 10.04 FRR1343 pKa = 11.84 RR1344 pKa = 11.84 LRR1346 pKa = 11.84 GLARR1350 pKa = 11.84 KK1351 pKa = 9.26 ARR1353 pKa = 11.84 ARR1355 pKa = 11.84 RR1356 pKa = 11.84 FDD1358 pKa = 3.24 NDD1360 pKa = 3.69 GIMEE1364 pKa = 4.35 IRR1366 pKa = 11.84 QALLKK1371 pKa = 10.83 KK1372 pKa = 9.9 EE1373 pKa = 4.93 DD1374 pKa = 3.99 KK1375 pKa = 10.88 DD1376 pKa = 3.6 EE1377 pKa = 4.91 HH1378 pKa = 8.34 DD1379 pKa = 3.58 LMNYY1383 pKa = 8.53 FVKK1386 pKa = 10.94 NKK1388 pKa = 10.18
Molecular weight: 155.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.087
IPC2_protein 7.293
IPC_protein 7.044
Toseland 6.766
ProMoST 7.732
Dawson 7.746
Bjellqvist 8.287
Wikipedia 7.658
Rodwell 7.775
Grimsley 6.839
Solomon 7.761
Lehninger 7.79
Nozaki 8.595
DTASelect 7.951
Thurlkill 7.995
EMBOSS 7.98
Sillero 8.361
Patrickios 3.808
IPC_peptide 7.761
IPC2_peptide 7.541
IPC2.peptide.svr19 7.278
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
5841
498
3955
1947.0
220.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.691 ± 0.711
3.065 ± 0.564
5.324 ± 0.415
6.403 ± 0.483
4.16 ± 0.168
4.674 ± 1.015
2.431 ± 0.464
5.855 ± 0.542
6.917 ± 0.687
10.871 ± 0.439
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.209 ± 0.241
5.068 ± 0.08
3.27 ± 0.161
3.647 ± 0.177
4.794 ± 0.328
8.851 ± 0.571
6.489 ± 0.281
7.105 ± 0.486
1.318 ± 0.364
2.859 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here