Amino acid dipepetide frequency for Microbacterium phage Fireman

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.256AlaAla: 17.256 ± 1.585
0.688AlaCys: 0.688 ± 0.265
7.854AlaAsp: 7.854 ± 0.904
8.026AlaGlu: 8.026 ± 1.052
3.153AlaPhe: 3.153 ± 0.425
10.09AlaGly: 10.09 ± 0.799
2.465AlaHis: 2.465 ± 0.442
5.504AlaIle: 5.504 ± 0.695
4.07AlaLys: 4.07 ± 0.499
11.466AlaLeu: 11.466 ± 1.163
3.44AlaMet: 3.44 ± 0.419
3.038AlaAsn: 3.038 ± 0.385
6.249AlaPro: 6.249 ± 0.651
3.038AlaGln: 3.038 ± 0.452
8.599AlaArg: 8.599 ± 0.891
6.708AlaSer: 6.708 ± 0.729
7.682AlaThr: 7.682 ± 0.759
7.224AlaVal: 7.224 ± 0.714
2.58AlaTrp: 2.58 ± 0.419
2.121AlaTyr: 2.121 ± 0.319
0.0AlaXaa: 0.0 ± 0.0
Cys
0.688CysAla: 0.688 ± 0.235
0.229CysCys: 0.229 ± 0.142
1.204CysAsp: 1.204 ± 0.33
0.459CysGlu: 0.459 ± 0.185
0.172CysPhe: 0.172 ± 0.094
1.204CysGly: 1.204 ± 0.303
0.459CysHis: 0.459 ± 0.167
0.344CysIle: 0.344 ± 0.14
0.115CysLys: 0.115 ± 0.09
0.401CysLeu: 0.401 ± 0.15
0.115CysMet: 0.115 ± 0.088
0.115CysAsn: 0.115 ± 0.086
0.573CysPro: 0.573 ± 0.196
0.115CysGln: 0.115 ± 0.086
0.516CysArg: 0.516 ± 0.204
0.229CysSer: 0.229 ± 0.101
0.459CysThr: 0.459 ± 0.165
0.631CysVal: 0.631 ± 0.195
0.115CysTrp: 0.115 ± 0.08
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
8.771AspAla: 8.771 ± 0.69
0.516AspCys: 0.516 ± 0.224
4.873AspAsp: 4.873 ± 0.628
4.357AspGlu: 4.357 ± 0.703
1.548AspPhe: 1.548 ± 0.25
6.593AspGly: 6.593 ± 0.671
1.089AspHis: 1.089 ± 0.301
2.637AspIle: 2.637 ± 0.338
1.204AspLys: 1.204 ± 0.249
5.504AspLeu: 5.504 ± 0.665
1.548AspMet: 1.548 ± 0.312
2.121AspAsn: 2.121 ± 0.32
3.497AspPro: 3.497 ± 0.544
2.58AspGln: 2.58 ± 0.309
4.988AspArg: 4.988 ± 0.629
2.981AspSer: 2.981 ± 0.366
3.554AspThr: 3.554 ± 0.482
4.529AspVal: 4.529 ± 0.604
1.204AspTrp: 1.204 ± 0.27
1.605AspTyr: 1.605 ± 0.284
0.0AspXaa: 0.0 ± 0.0
Glu
7.911GluAla: 7.911 ± 0.902
0.459GluCys: 0.459 ± 0.157
3.956GluAsp: 3.956 ± 0.618
4.3GluGlu: 4.3 ± 0.779
1.548GluPhe: 1.548 ± 0.314
4.586GluGly: 4.586 ± 0.774
1.892GluHis: 1.892 ± 0.371
1.319GluIle: 1.319 ± 0.41
1.548GluLys: 1.548 ± 0.336
3.44GluLeu: 3.44 ± 0.454
1.147GluMet: 1.147 ± 0.245
1.548GluAsn: 1.548 ± 0.381
4.529GluPro: 4.529 ± 0.634
2.179GluGln: 2.179 ± 0.379
4.873GluArg: 4.873 ± 0.587
2.924GluSer: 2.924 ± 0.423
3.898GluThr: 3.898 ± 0.589
6.134GluVal: 6.134 ± 0.677
1.663GluTrp: 1.663 ± 0.318
1.548GluTyr: 1.548 ± 0.365
0.0GluXaa: 0.0 ± 0.0
Phe
3.038PheAla: 3.038 ± 0.424
0.287PheCys: 0.287 ± 0.146
2.236PheAsp: 2.236 ± 0.418
1.548PheGlu: 1.548 ± 0.323
0.516PhePhe: 0.516 ± 0.167
2.121PheGly: 2.121 ± 0.335
0.287PheHis: 0.287 ± 0.115
1.261PheIle: 1.261 ± 0.292
0.516PheLys: 0.516 ± 0.19
1.835PheLeu: 1.835 ± 0.315
0.688PheMet: 0.688 ± 0.188
0.516PheAsn: 0.516 ± 0.159
0.803PhePro: 0.803 ± 0.238
0.745PheGln: 0.745 ± 0.253
1.376PheArg: 1.376 ± 0.287
1.204PheSer: 1.204 ± 0.234
2.694PheThr: 2.694 ± 0.427
2.236PheVal: 2.236 ± 0.351
0.401PheTrp: 0.401 ± 0.143
0.688PheTyr: 0.688 ± 0.196
0.0PheXaa: 0.0 ± 0.0
Gly
7.911GlyAla: 7.911 ± 0.813
0.573GlyCys: 0.573 ± 0.257
6.364GlyAsp: 6.364 ± 0.653
5.274GlyGlu: 5.274 ± 0.554
2.637GlyPhe: 2.637 ± 0.388
7.911GlyGly: 7.911 ± 0.591
2.064GlyHis: 2.064 ± 0.381
4.472GlyIle: 4.472 ± 0.643
2.465GlyLys: 2.465 ± 0.408
7.338GlyLeu: 7.338 ± 0.942
2.293GlyMet: 2.293 ± 0.403
1.949GlyAsn: 1.949 ± 0.378
3.44GlyPro: 3.44 ± 0.411
2.694GlyGln: 2.694 ± 0.393
4.988GlyArg: 4.988 ± 0.612
6.134GlySer: 6.134 ± 0.826
6.765GlyThr: 6.765 ± 0.875
6.421GlyVal: 6.421 ± 0.499
2.58GlyTrp: 2.58 ± 0.424
2.981GlyTyr: 2.981 ± 0.482
0.0GlyXaa: 0.0 ± 0.0
His
2.007HisAla: 2.007 ± 0.339
0.115HisCys: 0.115 ± 0.078
1.261HisAsp: 1.261 ± 0.261
1.261HisGlu: 1.261 ± 0.299
0.631HisPhe: 0.631 ± 0.179
1.892HisGly: 1.892 ± 0.37
0.516HisHis: 0.516 ± 0.17
0.459HisIle: 0.459 ± 0.153
0.516HisLys: 0.516 ± 0.173
2.465HisLeu: 2.465 ± 0.44
0.516HisMet: 0.516 ± 0.174
0.287HisAsn: 0.287 ± 0.146
1.376HisPro: 1.376 ± 0.325
0.287HisGln: 0.287 ± 0.127
1.835HisArg: 1.835 ± 0.381
1.089HisSer: 1.089 ± 0.25
1.433HisThr: 1.433 ± 0.281
1.319HisVal: 1.319 ± 0.289
0.229HisTrp: 0.229 ± 0.113
0.631HisTyr: 0.631 ± 0.189
0.0HisXaa: 0.0 ± 0.0
Ile
5.16IleAla: 5.16 ± 0.577
0.229IleCys: 0.229 ± 0.143
2.981IleAsp: 2.981 ± 0.401
3.726IleGlu: 3.726 ± 0.569
0.631IlePhe: 0.631 ± 0.16
4.185IleGly: 4.185 ± 0.501
0.745IleHis: 0.745 ± 0.209
2.523IleIle: 2.523 ± 0.347
1.204IleLys: 1.204 ± 0.225
2.637IleLeu: 2.637 ± 0.362
0.803IleMet: 0.803 ± 0.181
0.975IleAsn: 0.975 ± 0.286
3.325IlePro: 3.325 ± 0.602
1.319IleGln: 1.319 ± 0.301
2.866IleArg: 2.866 ± 0.498
2.351IleSer: 2.351 ± 0.429
4.07IleThr: 4.07 ± 0.555
3.956IleVal: 3.956 ± 0.469
0.917IleTrp: 0.917 ± 0.198
0.86IleTyr: 0.86 ± 0.212
0.0IleXaa: 0.0 ± 0.0
Lys
3.612LysAla: 3.612 ± 0.409
0.287LysCys: 0.287 ± 0.131
1.261LysAsp: 1.261 ± 0.231
0.975LysGlu: 0.975 ± 0.236
0.688LysPhe: 0.688 ± 0.211
2.637LysGly: 2.637 ± 0.371
0.516LysHis: 0.516 ± 0.185
0.917LysIle: 0.917 ± 0.226
0.745LysLys: 0.745 ± 0.274
0.917LysLeu: 0.917 ± 0.272
0.401LysMet: 0.401 ± 0.13
0.631LysAsn: 0.631 ± 0.203
1.663LysPro: 1.663 ± 0.328
1.204LysGln: 1.204 ± 0.209
2.694LysArg: 2.694 ± 0.507
1.892LysSer: 1.892 ± 0.358
0.745LysThr: 0.745 ± 0.204
3.21LysVal: 3.21 ± 0.442
0.401LysTrp: 0.401 ± 0.157
0.745LysTyr: 0.745 ± 0.193
0.0LysXaa: 0.0 ± 0.0
Leu
9.975LeuAla: 9.975 ± 0.907
0.401LeuCys: 0.401 ± 0.163
5.848LeuAsp: 5.848 ± 0.542
4.07LeuGlu: 4.07 ± 0.6
1.605LeuPhe: 1.605 ± 0.318
7.109LeuGly: 7.109 ± 0.948
1.147LeuHis: 1.147 ± 0.287
4.357LeuIle: 4.357 ± 0.593
1.147LeuLys: 1.147 ± 0.292
5.905LeuLeu: 5.905 ± 0.761
1.663LeuMet: 1.663 ± 0.307
2.121LeuAsn: 2.121 ± 0.297
4.529LeuPro: 4.529 ± 0.446
2.179LeuGln: 2.179 ± 0.36
5.217LeuArg: 5.217 ± 0.688
4.414LeuSer: 4.414 ± 0.569
6.994LeuThr: 6.994 ± 0.549
6.306LeuVal: 6.306 ± 0.701
1.491LeuTrp: 1.491 ± 0.299
1.319LeuTyr: 1.319 ± 0.283
0.0LeuXaa: 0.0 ± 0.0
Met
2.752MetAla: 2.752 ± 0.433
0.172MetCys: 0.172 ± 0.112
1.261MetAsp: 1.261 ± 0.278
0.803MetGlu: 0.803 ± 0.19
0.631MetPhe: 0.631 ± 0.216
2.064MetGly: 2.064 ± 0.379
0.631MetHis: 0.631 ± 0.209
1.376MetIle: 1.376 ± 0.264
0.459MetLys: 0.459 ± 0.137
1.777MetLeu: 1.777 ± 0.32
0.287MetMet: 0.287 ± 0.124
0.803MetAsn: 0.803 ± 0.175
1.433MetPro: 1.433 ± 0.314
0.803MetGln: 0.803 ± 0.192
1.319MetArg: 1.319 ± 0.275
2.694MetSer: 2.694 ± 0.364
3.038MetThr: 3.038 ± 0.424
1.089MetVal: 1.089 ± 0.226
0.401MetTrp: 0.401 ± 0.134
0.745MetTyr: 0.745 ± 0.221
0.0MetXaa: 0.0 ± 0.0
Asn
3.268AsnAla: 3.268 ± 0.342
0.401AsnCys: 0.401 ± 0.185
0.975AsnAsp: 0.975 ± 0.179
0.975AsnGlu: 0.975 ± 0.298
0.344AsnPhe: 0.344 ± 0.172
3.21AsnGly: 3.21 ± 0.473
0.516AsnHis: 0.516 ± 0.164
0.86AsnIle: 0.86 ± 0.22
0.573AsnLys: 0.573 ± 0.172
3.153AsnLeu: 3.153 ± 0.383
0.344AsnMet: 0.344 ± 0.153
0.344AsnAsn: 0.344 ± 0.164
2.121AsnPro: 2.121 ± 0.396
0.745AsnGln: 0.745 ± 0.177
1.949AsnArg: 1.949 ± 0.32
1.663AsnSer: 1.663 ± 0.326
1.949AsnThr: 1.949 ± 0.3
1.605AsnVal: 1.605 ± 0.252
0.287AsnTrp: 0.287 ± 0.122
0.975AsnTyr: 0.975 ± 0.299
0.0AsnXaa: 0.0 ± 0.0
Pro
5.676ProAla: 5.676 ± 0.445
0.688ProCys: 0.688 ± 0.232
4.128ProAsp: 4.128 ± 0.642
4.644ProGlu: 4.644 ± 0.605
1.491ProPhe: 1.491 ± 0.282
5.618ProGly: 5.618 ± 0.561
1.491ProHis: 1.491 ± 0.307
1.777ProIle: 1.777 ± 0.317
1.835ProLys: 1.835 ± 0.354
3.325ProLeu: 3.325 ± 0.417
1.491ProMet: 1.491 ± 0.284
1.892ProAsn: 1.892 ± 0.37
4.07ProPro: 4.07 ± 0.512
2.351ProGln: 2.351 ± 0.488
3.038ProArg: 3.038 ± 0.457
3.554ProSer: 3.554 ± 0.469
4.07ProThr: 4.07 ± 0.699
4.93ProVal: 4.93 ± 0.695
0.86ProTrp: 0.86 ± 0.207
1.548ProTyr: 1.548 ± 0.238
0.0ProXaa: 0.0 ± 0.0
Gln
4.758GlnAla: 4.758 ± 0.68
0.172GlnCys: 0.172 ± 0.093
1.548GlnAsp: 1.548 ± 0.204
1.376GlnGlu: 1.376 ± 0.283
1.032GlnPhe: 1.032 ± 0.245
2.179GlnGly: 2.179 ± 0.382
0.917GlnHis: 0.917 ± 0.219
1.663GlnIle: 1.663 ± 0.32
0.459GlnLys: 0.459 ± 0.178
1.261GlnLeu: 1.261 ± 0.434
1.089GlnMet: 1.089 ± 0.241
1.089GlnAsn: 1.089 ± 0.242
1.949GlnPro: 1.949 ± 0.323
1.032GlnGln: 1.032 ± 0.266
2.866GlnArg: 2.866 ± 0.429
2.121GlnSer: 2.121 ± 0.36
1.777GlnThr: 1.777 ± 0.241
2.523GlnVal: 2.523 ± 0.432
0.516GlnTrp: 0.516 ± 0.214
1.032GlnTyr: 1.032 ± 0.198
0.0GlnXaa: 0.0 ± 0.0
Arg
8.485ArgAla: 8.485 ± 0.715
1.032ArgCys: 1.032 ± 0.255
4.529ArgAsp: 4.529 ± 0.594
4.07ArgGlu: 4.07 ± 0.514
1.892ArgPhe: 1.892 ± 0.34
5.389ArgGly: 5.389 ± 0.635
1.376ArgHis: 1.376 ± 0.301
3.497ArgIle: 3.497 ± 0.519
2.007ArgLys: 2.007 ± 0.355
6.077ArgLeu: 6.077 ± 0.561
2.236ArgMet: 2.236 ± 0.367
1.491ArgAsn: 1.491 ± 0.306
3.44ArgPro: 3.44 ± 0.456
3.096ArgGln: 3.096 ± 0.464
6.02ArgArg: 6.02 ± 0.857
3.153ArgSer: 3.153 ± 0.377
4.357ArgThr: 4.357 ± 0.558
4.988ArgVal: 4.988 ± 0.628
1.491ArgTrp: 1.491 ± 0.33
2.236ArgTyr: 2.236 ± 0.451
0.0ArgXaa: 0.0 ± 0.0
Ser
7.281SerAla: 7.281 ± 0.92
0.344SerCys: 0.344 ± 0.155
3.726SerAsp: 3.726 ± 0.438
3.382SerGlu: 3.382 ± 0.501
2.179SerPhe: 2.179 ± 0.437
4.988SerGly: 4.988 ± 0.778
1.147SerHis: 1.147 ± 0.234
3.268SerIle: 3.268 ± 0.465
1.548SerLys: 1.548 ± 0.268
3.898SerLeu: 3.898 ± 0.527
2.007SerMet: 2.007 ± 0.266
2.121SerAsn: 2.121 ± 0.399
3.038SerPro: 3.038 ± 0.452
1.147SerGln: 1.147 ± 0.277
3.554SerArg: 3.554 ± 0.44
2.752SerSer: 2.752 ± 0.678
4.357SerThr: 4.357 ± 0.54
4.242SerVal: 4.242 ± 0.57
0.803SerTrp: 0.803 ± 0.21
1.147SerTyr: 1.147 ± 0.28
0.0SerXaa: 0.0 ± 0.0
Thr
9.058ThrAla: 9.058 ± 0.978
0.287ThrCys: 0.287 ± 0.108
4.3ThrAsp: 4.3 ± 0.587
4.128ThrGlu: 4.128 ± 0.677
1.72ThrPhe: 1.72 ± 0.379
6.822ThrGly: 6.822 ± 0.759
1.089ThrHis: 1.089 ± 0.256
3.612ThrIle: 3.612 ± 0.401
1.949ThrLys: 1.949 ± 0.404
6.708ThrLeu: 6.708 ± 0.612
1.147ThrMet: 1.147 ± 0.241
2.064ThrAsn: 2.064 ± 0.303
5.16ThrPro: 5.16 ± 0.798
1.72ThrGln: 1.72 ± 0.289
4.3ThrArg: 4.3 ± 0.569
4.013ThrSer: 4.013 ± 0.553
4.529ThrThr: 4.529 ± 0.761
6.249ThrVal: 6.249 ± 0.675
1.204ThrTrp: 1.204 ± 0.261
1.548ThrTyr: 1.548 ± 0.332
0.0ThrXaa: 0.0 ± 0.0
Val
8.657ValAla: 8.657 ± 0.858
0.917ValCys: 0.917 ± 0.234
4.128ValAsp: 4.128 ± 0.432
5.102ValGlu: 5.102 ± 0.536
1.491ValPhe: 1.491 ± 0.313
5.217ValGly: 5.217 ± 0.658
1.089ValHis: 1.089 ± 0.24
4.414ValIle: 4.414 ± 0.456
2.408ValLys: 2.408 ± 0.373
6.306ValLeu: 6.306 ± 0.629
2.007ValMet: 2.007 ± 0.33
1.949ValAsn: 1.949 ± 0.282
5.217ValPro: 5.217 ± 0.623
2.58ValGln: 2.58 ± 0.467
5.733ValArg: 5.733 ± 0.667
4.472ValSer: 4.472 ± 0.456
6.708ValThr: 6.708 ± 0.7
6.02ValVal: 6.02 ± 0.649
1.605ValTrp: 1.605 ± 0.329
1.204ValTyr: 1.204 ± 0.322
0.0ValXaa: 0.0 ± 0.0
Trp
2.064TrpAla: 2.064 ± 0.309
0.287TrpCys: 0.287 ± 0.119
0.975TrpAsp: 0.975 ± 0.227
1.261TrpGlu: 1.261 ± 0.32
0.631TrpPhe: 0.631 ± 0.212
1.433TrpGly: 1.433 ± 0.293
0.401TrpHis: 0.401 ± 0.164
0.688TrpIle: 0.688 ± 0.243
0.917TrpLys: 0.917 ± 0.266
1.433TrpLeu: 1.433 ± 0.345
0.401TrpMet: 0.401 ± 0.163
0.745TrpAsn: 0.745 ± 0.175
0.917TrpPro: 0.917 ± 0.251
0.745TrpGln: 0.745 ± 0.163
1.72TrpArg: 1.72 ± 0.311
1.376TrpSer: 1.376 ± 0.31
1.089TrpThr: 1.089 ± 0.279
1.548TrpVal: 1.548 ± 0.298
0.745TrpTrp: 0.745 ± 0.227
0.803TrpTyr: 0.803 ± 0.237
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.924TyrAla: 2.924 ± 0.483
0.057TyrCys: 0.057 ± 0.063
2.293TyrAsp: 2.293 ± 0.371
1.663TyrGlu: 1.663 ± 0.337
0.459TyrPhe: 0.459 ± 0.19
2.007TyrGly: 2.007 ± 0.407
0.287TyrHis: 0.287 ± 0.114
0.516TyrIle: 0.516 ± 0.185
0.459TyrLys: 0.459 ± 0.157
1.949TyrLeu: 1.949 ± 0.373
0.86TyrMet: 0.86 ± 0.232
0.459TyrAsn: 0.459 ± 0.148
1.147TyrPro: 1.147 ± 0.241
0.803TyrGln: 0.803 ± 0.196
2.408TyrArg: 2.408 ± 0.419
1.204TyrSer: 1.204 ± 0.247
1.491TyrThr: 1.491 ± 0.287
2.064TyrVal: 2.064 ± 0.311
0.688TyrTrp: 0.688 ± 0.203
0.573TyrTyr: 0.573 ± 0.18
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (17444 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski