Amino acid dipepetide frequency for Bifidobacterium sp. LMG 31471

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.13AlaAla: 18.13 ± 0.271	1.078AlaCys: 1.078 ± 0.037	8.943AlaAsp: 8.943 ± 0.139	5.968AlaGlu: 5.968 ± 0.1	3.435AlaPhe: 3.435 ± 0.082	10.532AlaGly: 10.532 ± 0.153	2.141AlaHis: 2.141 ± 0.057	5.1AlaIle: 5.1 ± 0.082	5.286AlaLys: 5.286 ± 0.119	9.945AlaLeu: 9.945 ± 0.136	3.2AlaMet: 3.2 ± 0.067	3.559AlaAsn: 3.559 ± 0.073	4.483AlaPro: 4.483 ± 0.103	4.547AlaGln: 4.547 ± 0.103	6.184AlaArg: 6.184 ± 0.116	6.975AlaSer: 6.975 ± 0.101	7.24AlaThr: 7.24 ± 0.133	9.345AlaVal: 9.345 ± 0.13	1.517AlaTrp: 1.517 ± 0.047	2.891AlaTyr: 2.891 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	1.058CysAla: 1.058 ± 0.037	0.116CysCys: 0.116 ± 0.013	0.565CysAsp: 0.565 ± 0.029	0.389CysGlu: 0.389 ± 0.019	0.253CysPhe: 0.253 ± 0.017	0.989CysGly: 0.989 ± 0.045	0.185CysHis: 0.185 ± 0.016	0.358CysIle: 0.358 ± 0.025	0.194CysLys: 0.194 ± 0.015	0.767CysLeu: 0.767 ± 0.033	0.248CysMet: 0.248 ± 0.018	0.213CysAsn: 0.213 ± 0.018	0.417CysPro: 0.417 ± 0.024	0.2CysGln: 0.2 ± 0.018	0.605CysArg: 0.605 ± 0.031	0.503CysSer: 0.503 ± 0.027	0.535CysThr: 0.535 ± 0.025	0.74CysVal: 0.74 ± 0.033	0.127CysTrp: 0.127 ± 0.013	0.208CysTyr: 0.208 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	8.625AspAla: 8.625 ± 0.132	0.525AspCys: 0.525 ± 0.027	5.573AspAsp: 5.573 ± 0.117	4.52AspGlu: 4.52 ± 0.078	2.111AspPhe: 2.111 ± 0.055	7.477AspGly: 7.477 ± 0.128	1.36AspHis: 1.36 ± 0.041	3.107AspIle: 3.107 ± 0.064	2.55AspLys: 2.55 ± 0.076	5.082AspLeu: 5.082 ± 0.088	1.641AspMet: 1.641 ± 0.052	1.887AspAsn: 1.887 ± 0.053	3.446AspPro: 3.446 ± 0.07	1.775AspGln: 1.775 ± 0.045	3.633AspArg: 3.633 ± 0.089	3.791AspSer: 3.791 ± 0.08	3.837AspThr: 3.837 ± 0.08	5.54AspVal: 5.54 ± 0.089	0.983AspTrp: 0.983 ± 0.042	2.069AspTyr: 2.069 ± 0.058	0.0AspXaa: 0.0 ± 0.0
Glu	5.523GluAla: 5.523 ± 0.099	0.414GluCys: 0.414 ± 0.026	2.874GluAsp: 2.874 ± 0.057	2.713GluGlu: 2.713 ± 0.076	1.577GluPhe: 1.577 ± 0.055	3.412GluGly: 3.412 ± 0.076	1.519GluHis: 1.519 ± 0.044	2.338GluIle: 2.338 ± 0.061	1.851GluLys: 1.851 ± 0.051	4.624GluLeu: 4.624 ± 0.092	1.161GluMet: 1.161 ± 0.042	1.637GluAsn: 1.637 ± 0.049	2.661GluPro: 2.661 ± 0.057	2.324GluGln: 2.324 ± 0.061	4.179GluArg: 4.179 ± 0.102	2.981GluSer: 2.981 ± 0.065	3.41GluThr: 3.41 ± 0.072	3.161GluVal: 3.161 ± 0.071	0.576GluTrp: 0.576 ± 0.027	1.665GluTyr: 1.665 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	3.936PheAla: 3.936 ± 0.069	0.302PheCys: 0.302 ± 0.019	2.68PheAsp: 2.68 ± 0.055	1.517PheGlu: 1.517 ± 0.049	1.007PhePhe: 1.007 ± 0.046	3.114PheGly: 3.114 ± 0.08	0.69PheHis: 0.69 ± 0.033	1.52PheIle: 1.52 ± 0.049	0.978PheLys: 0.978 ± 0.041	2.39PheLeu: 2.39 ± 0.058	0.739PheMet: 0.739 ± 0.036	1.044PheAsn: 1.044 ± 0.035	1.303PhePro: 1.303 ± 0.046	0.81PheGln: 0.81 ± 0.032	1.631PheArg: 1.631 ± 0.048	2.061PheSer: 2.061 ± 0.054	2.26PheThr: 2.26 ± 0.052	2.495PheVal: 2.495 ± 0.071	0.388PheTrp: 0.388 ± 0.024	0.853PheTyr: 0.853 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	8.291GlyAla: 8.291 ± 0.136	0.765GlyCys: 0.765 ± 0.032	5.678GlyAsp: 5.678 ± 0.093	4.651GlyGlu: 4.651 ± 0.079	3.027GlyPhe: 3.027 ± 0.069	6.928GlyGly: 6.928 ± 0.126	1.64GlyHis: 1.64 ± 0.043	4.267GlyIle: 4.267 ± 0.073	4.227GlyLys: 4.227 ± 0.095	6.911GlyLeu: 6.911 ± 0.099	2.16GlyMet: 2.16 ± 0.055	2.983GlyAsn: 2.983 ± 0.079	2.493GlyPro: 2.493 ± 0.067	2.555GlyGln: 2.555 ± 0.067	5.237GlyArg: 5.237 ± 0.085	5.99GlySer: 5.99 ± 0.131	5.853GlyThr: 5.853 ± 0.113	6.715GlyVal: 6.715 ± 0.098	1.254GlyTrp: 1.254 ± 0.042	2.718GlyTyr: 2.718 ± 0.07	0.0GlyXaa: 0.0 ± 0.0
His	2.296HisAla: 2.296 ± 0.06	0.211HisCys: 0.211 ± 0.016	1.731HisAsp: 1.731 ± 0.047	1.06HisGlu: 1.06 ± 0.041	0.529HisPhe: 0.529 ± 0.027	2.092HisGly: 2.092 ± 0.057	0.521HisHis: 0.521 ± 0.028	0.988HisIle: 0.988 ± 0.036	0.546HisLys: 0.546 ± 0.026	1.42HisLeu: 1.42 ± 0.044	0.55HisMet: 0.55 ± 0.026	0.521HisAsn: 0.521 ± 0.024	1.276HisPro: 1.276 ± 0.044	0.518HisGln: 0.518 ± 0.024	1.344HisArg: 1.344 ± 0.046	0.931HisSer: 0.931 ± 0.034	1.142HisThr: 1.142 ± 0.034	1.787HisVal: 1.787 ± 0.051	0.312HisTrp: 0.312 ± 0.023	0.543HisTyr: 0.543 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.294IleAla: 6.294 ± 0.087	0.47IleCys: 0.47 ± 0.023	3.924IleAsp: 3.924 ± 0.071	2.629IleGlu: 2.629 ± 0.062	1.135IlePhe: 1.135 ± 0.044	4.16IleGly: 4.16 ± 0.089	0.844IleHis: 0.844 ± 0.035	2.538IleIle: 2.538 ± 0.062	1.524IleLys: 1.524 ± 0.053	3.231IleLeu: 3.231 ± 0.078	1.091IleMet: 1.091 ± 0.045	1.536IleAsn: 1.536 ± 0.044	2.467IlePro: 2.467 ± 0.056	1.15IleGln: 1.15 ± 0.038	2.844IleArg: 2.844 ± 0.066	2.625IleSer: 2.625 ± 0.067	3.198IleThr: 3.198 ± 0.067	4.258IleVal: 4.258 ± 0.088	0.497IleTrp: 0.497 ± 0.024	1.026IleTyr: 1.026 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	5.154LysAla: 5.154 ± 0.124	0.159LysCys: 0.159 ± 0.014	2.597LysAsp: 2.597 ± 0.074	1.856LysGlu: 1.856 ± 0.05	0.945LysPhe: 0.945 ± 0.033	2.725LysGly: 2.725 ± 0.067	0.694LysHis: 0.694 ± 0.027	1.552LysIle: 1.552 ± 0.055	1.705LysLys: 1.705 ± 0.058	3.217LysLeu: 3.217 ± 0.097	0.723LysMet: 0.723 ± 0.036	1.359LysAsn: 1.359 ± 0.047	2.506LysPro: 2.506 ± 0.059	1.407LysGln: 1.407 ± 0.046	2.311LysArg: 2.311 ± 0.058	2.332LysSer: 2.332 ± 0.064	3.062LysThr: 3.062 ± 0.09	2.979LysVal: 2.979 ± 0.087	0.381LysTrp: 0.381 ± 0.02	1.046LysTyr: 1.046 ± 0.04	0.0LysXaa: 0.0 ± 0.0
Leu	10.366LeuAla: 10.366 ± 0.138	0.885LeuCys: 0.885 ± 0.033	5.98LeuAsp: 5.98 ± 0.097	3.863LeuGlu: 3.863 ± 0.091	2.826LeuPhe: 2.826 ± 0.076	6.57LeuGly: 6.57 ± 0.102	1.765LeuHis: 1.765 ± 0.052	4.11LeuIle: 4.11 ± 0.086	3.389LeuLys: 3.389 ± 0.075	7.591LeuLeu: 7.591 ± 0.139	2.113LeuMet: 2.113 ± 0.068	2.647LeuAsn: 2.647 ± 0.055	4.384LeuPro: 4.384 ± 0.069	2.333LeuGln: 2.333 ± 0.057	5.174LeuArg: 5.174 ± 0.105	5.013LeuSer: 5.013 ± 0.077	5.747LeuThr: 5.747 ± 0.102	5.944LeuVal: 5.944 ± 0.105	0.99LeuTrp: 0.99 ± 0.037	2.065LeuTyr: 2.065 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	2.699MetAla: 2.699 ± 0.06	0.253MetCys: 0.253 ± 0.021	1.295MetAsp: 1.295 ± 0.043	0.984MetGlu: 0.984 ± 0.036	0.824MetPhe: 0.824 ± 0.032	1.782MetGly: 1.782 ± 0.051	0.49MetHis: 0.49 ± 0.021	1.238MetIle: 1.238 ± 0.047	0.885MetLys: 0.885 ± 0.032	2.444MetLeu: 2.444 ± 0.058	0.759MetMet: 0.759 ± 0.033	0.857MetAsn: 0.857 ± 0.034	1.469MetPro: 1.469 ± 0.049	0.834MetGln: 0.834 ± 0.031	1.724MetArg: 1.724 ± 0.055	1.747MetSer: 1.747 ± 0.042	2.067MetThr: 2.067 ± 0.051	1.731MetVal: 1.731 ± 0.047	0.27MetTrp: 0.27 ± 0.019	0.541MetTyr: 0.541 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.79AsnAla: 3.79 ± 0.075	0.183AsnCys: 0.183 ± 0.016	2.201AsnAsp: 2.201 ± 0.059	1.558AsnGlu: 1.558 ± 0.047	0.811AsnPhe: 0.811 ± 0.033	3.586AsnGly: 3.586 ± 0.086	0.593AsnHis: 0.593 ± 0.027	1.391AsnIle: 1.391 ± 0.048	1.176AsnLys: 1.176 ± 0.045	2.392AsnLeu: 2.392 ± 0.053	0.709AsnMet: 0.709 ± 0.031	1.065AsnAsn: 1.065 ± 0.044	2.045AsnPro: 2.045 ± 0.053	0.923AsnGln: 0.923 ± 0.033	1.804AsnArg: 1.804 ± 0.055	1.555AsnSer: 1.555 ± 0.05	2.091AsnThr: 2.091 ± 0.06	2.457AsnVal: 2.457 ± 0.054	0.44AsnTrp: 0.44 ± 0.025	0.831AsnTyr: 0.831 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	6.064ProAla: 6.064 ± 0.129	0.317ProCys: 0.317 ± 0.021	3.697ProAsp: 3.697 ± 0.082	2.927ProGlu: 2.927 ± 0.074	1.596ProPhe: 1.596 ± 0.045	3.816ProGly: 3.816 ± 0.073	0.98ProHis: 0.98 ± 0.038	2.008ProIle: 2.008 ± 0.055	1.799ProLys: 1.799 ± 0.048	3.673ProLeu: 3.673 ± 0.081	1.091ProMet: 1.091 ± 0.042	1.45ProAsn: 1.45 ± 0.045	1.367ProPro: 1.367 ± 0.048	1.947ProGln: 1.947 ± 0.065	2.411ProArg: 2.411 ± 0.071	3.004ProSer: 3.004 ± 0.078	3.018ProThr: 3.018 ± 0.065	3.95ProVal: 3.95 ± 0.07	0.701ProTrp: 0.701 ± 0.027	1.405ProTyr: 1.405 ± 0.05	0.0ProXaa: 0.0 ± 0.0
Gln	3.976GlnAla: 3.976 ± 0.093	0.28GlnCys: 0.28 ± 0.021	1.584GlnAsp: 1.584 ± 0.049	1.348GlnGlu: 1.348 ± 0.042	0.969GlnPhe: 0.969 ± 0.034	2.196GlnGly: 2.196 ± 0.052	0.672GlnHis: 0.672 ± 0.028	1.624GlnIle: 1.624 ± 0.047	1.025GlnLys: 1.025 ± 0.039	3.119GlnLeu: 3.119 ± 0.063	0.844GlnMet: 0.844 ± 0.029	0.955GlnAsn: 0.955 ± 0.031	2.053GlnPro: 2.053 ± 0.076	1.773GlnGln: 1.773 ± 0.062	2.247GlnArg: 2.247 ± 0.058	2.263GlnSer: 2.263 ± 0.053	2.105GlnThr: 2.105 ± 0.051	2.392GlnVal: 2.392 ± 0.062	0.604GlnTrp: 0.604 ± 0.026	1.139GlnTyr: 1.139 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	5.535ArgAla: 5.535 ± 0.103	0.509ArgCys: 0.509 ± 0.027	3.758ArgAsp: 3.758 ± 0.076	3.437ArgGlu: 3.437 ± 0.074	2.354ArgPhe: 2.354 ± 0.06	3.976ArgGly: 3.976 ± 0.088	1.625ArgHis: 1.625 ± 0.052	3.38ArgIle: 3.38 ± 0.058	2.467ArgLys: 2.467 ± 0.07	5.721ArgLeu: 5.721 ± 0.114	1.944ArgMet: 1.944 ± 0.051	2.029ArgAsn: 2.029 ± 0.053	2.748ArgPro: 2.748 ± 0.069	2.34ArgGln: 2.34 ± 0.066	5.425ArgArg: 5.425 ± 0.137	3.299ArgSer: 3.299 ± 0.079	3.576ArgThr: 3.576 ± 0.071	3.988ArgVal: 3.988 ± 0.088	0.878ArgTrp: 0.878 ± 0.037	2.045ArgTyr: 2.045 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	7.176SerAla: 7.176 ± 0.102	0.458SerCys: 0.458 ± 0.03	4.067SerAsp: 4.067 ± 0.088	2.735SerGlu: 2.735 ± 0.063	1.958SerPhe: 1.958 ± 0.057	6.371SerGly: 6.371 ± 0.145	1.198SerHis: 1.198 ± 0.038	2.789SerIle: 2.789 ± 0.058	2.202SerLys: 2.202 ± 0.067	5.084SerLeu: 5.084 ± 0.081	1.518SerMet: 1.518 ± 0.045	1.848SerAsn: 1.848 ± 0.056	2.629SerPro: 2.629 ± 0.064	2.117SerGln: 2.117 ± 0.051	3.668SerArg: 3.668 ± 0.078	4.26SerSer: 4.26 ± 0.114	3.712SerThr: 3.712 ± 0.067	4.607SerVal: 4.607 ± 0.086	0.867SerTrp: 0.867 ± 0.03	1.557SerTyr: 1.557 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	8.452ThrAla: 8.452 ± 0.14	0.495ThrCys: 0.495 ± 0.026	4.199ThrAsp: 4.199 ± 0.086	2.564ThrGlu: 2.564 ± 0.06	2.258ThrPhe: 2.258 ± 0.063	5.734ThrGly: 5.734 ± 0.1	1.156ThrHis: 1.156 ± 0.04	3.433ThrIle: 3.433 ± 0.074	2.33ThrLys: 2.33 ± 0.077	6.096ThrLeu: 6.096 ± 0.096	1.461ThrMet: 1.461 ± 0.043	1.896ThrAsn: 1.896 ± 0.054	3.675ThrPro: 3.675 ± 0.077	2.085ThrGln: 2.085 ± 0.056	3.066ThrArg: 3.066 ± 0.077	3.782ThrSer: 3.782 ± 0.08	4.489ThrThr: 4.489 ± 0.089	6.741ThrVal: 6.741 ± 0.163	0.873ThrTrp: 0.873 ± 0.038	1.812ThrTyr: 1.812 ± 0.062	0.0ThrXaa: 0.0 ± 0.0
Val	8.896ValAla: 8.896 ± 0.132	0.808ValCys: 0.808 ± 0.037	5.325ValAsp: 5.325 ± 0.082	3.879ValGlu: 3.879 ± 0.076	2.661ValPhe: 2.661 ± 0.068	5.327ValGly: 5.327 ± 0.091	1.424ValHis: 1.424 ± 0.041	3.99ValIle: 3.99 ± 0.084	3.083ValLys: 3.083 ± 0.085	6.48ValLeu: 6.48 ± 0.117	1.969ValMet: 1.969 ± 0.056	2.673ValAsn: 2.673 ± 0.07	3.986ValPro: 3.986 ± 0.077	2.008ValGln: 2.008 ± 0.051	4.687ValArg: 4.687 ± 0.092	5.285ValSer: 5.285 ± 0.09	6.45ValThr: 6.45 ± 0.148	6.346ValVal: 6.346 ± 0.124	0.969ValTrp: 0.969 ± 0.038	1.899ValTyr: 1.899 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	1.131TrpAla: 1.131 ± 0.046	0.157TrpCys: 0.157 ± 0.013	0.782TrpAsp: 0.782 ± 0.032	0.48TrpGlu: 0.48 ± 0.028	0.53TrpPhe: 0.53 ± 0.023	0.879TrpGly: 0.879 ± 0.037	0.318TrpHis: 0.318 ± 0.021	0.63TrpIle: 0.63 ± 0.028	0.591TrpLys: 0.591 ± 0.026	1.366TrpLeu: 1.366 ± 0.047	0.413TrpMet: 0.413 ± 0.023	0.547TrpAsn: 0.547 ± 0.028	0.531TrpPro: 0.531 ± 0.025	0.593TrpGln: 0.593 ± 0.024	0.972TrpArg: 0.972 ± 0.035	0.885TrpSer: 0.885 ± 0.038	0.917TrpThr: 0.917 ± 0.042	0.865TrpVal: 0.865 ± 0.033	0.245TrpTrp: 0.245 ± 0.018	0.458TrpTyr: 0.458 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.105TyrAla: 3.105 ± 0.062	0.288TyrCys: 0.288 ± 0.021	2.08TyrAsp: 2.08 ± 0.058	1.522TyrGlu: 1.522 ± 0.043	0.913TyrPhe: 0.913 ± 0.035	2.671TyrGly: 2.671 ± 0.057	0.503TyrHis: 0.503 ± 0.023	1.079TyrIle: 1.079 ± 0.042	0.903TyrLys: 0.903 ± 0.036	2.203TyrLeu: 2.203 ± 0.057	0.56TyrMet: 0.56 ± 0.027	0.897TyrAsn: 0.897 ± 0.04	1.281TyrPro: 1.281 ± 0.044	0.93TyrGln: 0.93 ± 0.034	1.887TyrArg: 1.887 ± 0.047	1.541TyrSer: 1.541 ± 0.047	1.889TyrThr: 1.889 ± 0.073	2.103TyrVal: 2.103 ± 0.057	0.417TyrTrp: 0.417 ± 0.022	0.869TyrTyr: 0.869 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2129 proteins (843209 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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