Pediococcus pentosaceus (strain ATCC 25745 / CCUG 21536 / LMG 10740 / 183-1w)
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1755 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q03FI2|Q03FI2_PEDPA Uncharacterized protein OS=Pediococcus pentosaceus (strain ATCC 25745 / CCUG 21536 / LMG 10740 / 183-1w) OX=278197 GN=PEPE_0984 PE=4 SV=1
MM1 pKa = 7.53 AANLEE6 pKa = 4.35 TLKK9 pKa = 11.03 SSLRR13 pKa = 11.84 IDD15 pKa = 4.51 GDD17 pKa = 4.46 DD18 pKa = 4.8 DD19 pKa = 5.61 DD20 pKa = 5.66 EE21 pKa = 4.48 LLKK24 pKa = 11.0 GYY26 pKa = 10.65 LSAATSYY33 pKa = 10.39 IKK35 pKa = 10.34 QAIGDD40 pKa = 4.1 EE41 pKa = 4.18 NSVPGFYY48 pKa = 9.36 EE49 pKa = 4.03 TEE51 pKa = 4.08 GVSNLFEE58 pKa = 4.06 TAVYY62 pKa = 10.69 ALAGSYY68 pKa = 8.51 WYY70 pKa = 10.35 YY71 pKa = 9.23 RR72 pKa = 11.84 TSITSNTVNPVDD84 pKa = 4.5 LVVDD88 pKa = 4.49 SIIGQLRR95 pKa = 11.84 GLYY98 pKa = 8.62 NQKK101 pKa = 9.91 QDD103 pKa = 3.44 EE104 pKa = 4.57 VNDD107 pKa = 3.71 NGNN110 pKa = 3.11
Molecular weight: 12.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.785
IPC2_protein 3.999
IPC_protein 3.935
Toseland 3.732
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.757
Grimsley 3.643
Solomon 3.91
Lehninger 3.859
Nozaki 4.037
DTASelect 4.253
Thurlkill 3.783
EMBOSS 3.859
Sillero 4.05
Patrickios 1.926
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.948
Protein with the highest isoelectric point:
>tr|Q03D65|Q03D65_PEDPA Putative tyrosine permease NhaC family OS=Pediococcus pentosaceus (strain ATCC 25745 / CCUG 21536 / LMG 10740 / 183-1w) OX=278197 GN=PEPE_1838 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.69 KK9 pKa = 7.77 RR10 pKa = 11.84 HH11 pKa = 4.99 RR12 pKa = 11.84 QRR14 pKa = 11.84 VHH16 pKa = 6.07 GFRR19 pKa = 11.84 KK20 pKa = 10.03 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.39 GRR39 pKa = 11.84 KK40 pKa = 8.52 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1755
0
1755
535200
15
3017
305.0
34.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.39 ± 0.061
0.409 ± 0.012
5.888 ± 0.074
6.136 ± 0.069
4.32 ± 0.057
6.679 ± 0.07
1.973 ± 0.025
7.738 ± 0.054
6.901 ± 0.057
9.327 ± 0.082
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.754 ± 0.033
5.24 ± 0.04
3.409 ± 0.039
4.275 ± 0.054
3.928 ± 0.048
6.175 ± 0.169
5.89 ± 0.079
7.129 ± 0.051
0.974 ± 0.022
3.465 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here