LeviOr01 phage
Average proteome isoelectric point is 8.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U6U245|A0A1U6U245_9VIRU Putative maturation protein OS=LeviOr01 phage OX=1927956 GN=mat PE=4 SV=1
MM1 pKa = 7.13 HH2 pKa = 7.56 TLTAQVTSALLQDD15 pKa = 4.94 LGLEE19 pKa = 4.19 SLPKK23 pKa = 10.17 EE24 pKa = 4.29 FPDD27 pKa = 4.37 RR28 pKa = 11.84 DD29 pKa = 3.77 SEE31 pKa = 4.61 FPARR35 pKa = 11.84 YY36 pKa = 8.49 LAYY39 pKa = 10.34 NFVRR43 pKa = 11.84 KK44 pKa = 10.24 LEE46 pKa = 4.24 PFSRR50 pKa = 11.84 KK51 pKa = 7.42 TDD53 pKa = 3.26 VPVAVLRR60 pKa = 11.84 NSLTTFMEE68 pKa = 3.94 AEE70 pKa = 4.05 YY71 pKa = 10.21 RR72 pKa = 11.84 CRR74 pKa = 11.84 VVNHH78 pKa = 6.67 HH79 pKa = 5.58 GRR81 pKa = 11.84 MYY83 pKa = 10.45 SALRR87 pKa = 11.84 EE88 pKa = 3.85 EE89 pKa = 4.33 DD90 pKa = 2.81 RR91 pKa = 11.84 SYY93 pKa = 10.13 FTEE96 pKa = 4.13 AFRR99 pKa = 11.84 LAKK102 pKa = 9.92 IWINQTMSGYY112 pKa = 8.71 WPRR115 pKa = 11.84 WEE117 pKa = 3.92 NARR120 pKa = 11.84 YY121 pKa = 7.96 TGGASRR127 pKa = 11.84 NCSRR131 pKa = 11.84 DD132 pKa = 3.01 RR133 pKa = 11.84 SLPALKK139 pKa = 9.86 WSGYY143 pKa = 8.56 AEE145 pKa = 4.08 RR146 pKa = 11.84 SNLSTTRR153 pKa = 11.84 PALAVVNDD161 pKa = 3.89 YY162 pKa = 10.78 IKK164 pKa = 10.93 PEE166 pKa = 4.17 FSPSDD171 pKa = 3.08 WRR173 pKa = 11.84 EE174 pKa = 3.4 RR175 pKa = 11.84 DD176 pKa = 3.02 VDD178 pKa = 3.62 IVDD181 pKa = 3.94 DD182 pKa = 3.97 SRR184 pKa = 11.84 FDD186 pKa = 3.98 FVAKK190 pKa = 8.53 TAKK193 pKa = 10.02 AVRR196 pKa = 11.84 FMAMEE201 pKa = 4.38 PEE203 pKa = 4.23 YY204 pKa = 11.8 NMLAQKK210 pKa = 10.65 CVGDD214 pKa = 4.75 CIRR217 pKa = 11.84 AALNAQGINLDD228 pKa = 3.73 DD229 pKa = 3.56 QRR231 pKa = 11.84 PNQEE235 pKa = 3.63 LAYY238 pKa = 9.89 LGSIFRR244 pKa = 11.84 TRR246 pKa = 11.84 ATLDD250 pKa = 3.03 QSSASDD256 pKa = 4.38 CIALFLLRR264 pKa = 11.84 LLPEE268 pKa = 4.37 RR269 pKa = 11.84 VRR271 pKa = 11.84 DD272 pKa = 3.48 WVLACRR278 pKa = 11.84 TPATSVAGSRR288 pKa = 11.84 LVLEE292 pKa = 5.25 KK293 pKa = 10.72 VATMGNGFIFEE304 pKa = 4.28 LQSLIFAAFAHH315 pKa = 6.8 ACTQLSGGRR324 pKa = 11.84 EE325 pKa = 4.0 CDD327 pKa = 2.97 IAVYY331 pKa = 10.55 GDD333 pKa = 4.73 DD334 pKa = 4.84 IIVSTPVARR343 pKa = 11.84 PLMDD347 pKa = 3.35 TLEE350 pKa = 4.32 YY351 pKa = 10.85 YY352 pKa = 11.01 GLIPNMEE359 pKa = 4.02 KK360 pKa = 10.51 SYY362 pKa = 10.46 WDD364 pKa = 3.13 EE365 pKa = 4.4 DD366 pKa = 3.83 EE367 pKa = 5.05 PFRR370 pKa = 11.84 EE371 pKa = 4.44 SCGKK375 pKa = 9.49 HH376 pKa = 3.95 WFAGRR381 pKa = 11.84 DD382 pKa = 3.35 VTPFYY387 pKa = 11.15 VKK389 pKa = 10.55 EE390 pKa = 3.87 PLGPLRR396 pKa = 11.84 TLFRR400 pKa = 11.84 AYY402 pKa = 10.68 NGLKK406 pKa = 8.99 EE407 pKa = 3.85 WTMRR411 pKa = 11.84 TGIPLNRR418 pKa = 11.84 TLATILAAIPKK429 pKa = 8.38 KK430 pKa = 10.58 DD431 pKa = 3.42 RR432 pKa = 11.84 VIVPPSFSIDD442 pKa = 3.74 CGLHH446 pKa = 5.41 TPVSGCTFPKK456 pKa = 10.0 RR457 pKa = 11.84 VIRR460 pKa = 11.84 HH461 pKa = 4.55 GDD463 pKa = 2.53 IRR465 pKa = 11.84 YY466 pKa = 9.76 SFKK469 pKa = 11.08 CLVDD473 pKa = 3.31 TSEE476 pKa = 5.04 DD477 pKa = 3.27 VTARR481 pKa = 11.84 LDD483 pKa = 3.83 DD484 pKa = 4.2 EE485 pKa = 4.89 VKK487 pKa = 10.28 LRR489 pKa = 11.84 YY490 pKa = 8.71 WLFEE494 pKa = 4.24 PPAEE498 pKa = 3.95 LLPRR502 pKa = 11.84 HH503 pKa = 6.71 LYY505 pKa = 8.88 PASPLAGYY513 pKa = 8.97 PRR515 pKa = 11.84 EE516 pKa = 3.95 EE517 pKa = 3.75 KK518 pKa = 10.38 RR519 pKa = 11.84 YY520 pKa = 9.29 VRR522 pKa = 11.84 GLSGEE527 pKa = 4.11 SRR529 pKa = 11.84 PEE531 pKa = 3.23 VWARR535 pKa = 11.84 RR536 pKa = 11.84 EE537 pKa = 4.2 AGPGDD542 pKa = 3.74 APP544 pKa = 4.18
Molecular weight: 61.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.755
IPC2_protein 7.0
IPC_protein 6.942
Toseland 6.664
ProMoST 7.556
Dawson 7.585
Bjellqvist 7.907
Wikipedia 7.497
Rodwell 7.6
Grimsley 6.722
Solomon 7.629
Lehninger 7.644
Nozaki 8.126
DTASelect 7.761
Thurlkill 7.79
EMBOSS 7.819
Sillero 8.097
Patrickios 4.291
IPC_peptide 7.629
IPC2_peptide 7.307
IPC2.peptide.svr19 7.176
Protein with the highest isoelectric point:
>tr|A0A1U6U260|A0A1U6U260_9VIRU Putative coat protein OS=LeviOr01 phage OX=1927956 GN=cp PE=4 SV=1
MM1 pKa = 7.95 RR2 pKa = 11.84 SEE4 pKa = 3.96 TQEE7 pKa = 3.77 WEE9 pKa = 4.88 IYY11 pKa = 8.98 PCWHH15 pKa = 6.74 GKK17 pKa = 8.24 PPKK20 pKa = 9.41 TSGPTVVDD28 pKa = 3.35 SKK30 pKa = 11.98 GNIDD34 pKa = 4.04 VSAIPCEE41 pKa = 4.33 TEE43 pKa = 3.88 SNAGVTKK50 pKa = 10.45 GNHH53 pKa = 5.18 VDD55 pKa = 3.94 ALPYY59 pKa = 9.77 WSDD62 pKa = 3.21 RR63 pKa = 11.84 LLVTVEE69 pKa = 3.58 PRR71 pKa = 11.84 VRR73 pKa = 11.84 NISYY77 pKa = 10.01 GVAVTRR83 pKa = 11.84 MPVPPPEE90 pKa = 4.16 NTCIPDD96 pKa = 3.79 GDD98 pKa = 4.14 PGGSKK103 pKa = 9.61 TIVRR107 pKa = 11.84 NKK109 pKa = 10.27 YY110 pKa = 10.7 SIDD113 pKa = 3.53 PFEE116 pKa = 4.18 WAAAPTFDD124 pKa = 5.01 LPEE127 pKa = 3.94 VSPAEE132 pKa = 4.06 LEE134 pKa = 4.36 FLRR137 pKa = 11.84 GMADD141 pKa = 3.14 TRR143 pKa = 11.84 ALASLNKK150 pKa = 10.49 AMVNLPMLFKK160 pKa = 10.55 EE161 pKa = 4.09 RR162 pKa = 11.84 RR163 pKa = 11.84 EE164 pKa = 4.14 TLKK167 pKa = 10.29 MAAGKK172 pKa = 10.66 VGVLADD178 pKa = 3.87 AARR181 pKa = 11.84 SIQQRR186 pKa = 11.84 DD187 pKa = 3.63 FSRR190 pKa = 11.84 YY191 pKa = 8.34 RR192 pKa = 11.84 KK193 pKa = 8.54 VAKK196 pKa = 9.6 RR197 pKa = 11.84 DD198 pKa = 3.29 RR199 pKa = 11.84 RR200 pKa = 11.84 RR201 pKa = 11.84 VARR204 pKa = 11.84 DD205 pKa = 2.92 IANEE209 pKa = 3.68 HH210 pKa = 6.78 LEE212 pKa = 5.36 FIFGWLPLIAEE223 pKa = 4.58 VEE225 pKa = 4.18 GAMDD229 pKa = 4.05 YY230 pKa = 10.52 IAQEE234 pKa = 3.72 RR235 pKa = 11.84 FEE237 pKa = 4.43 FIKK240 pKa = 10.96 GRR242 pKa = 11.84 GSHH245 pKa = 6.95 VITRR249 pKa = 11.84 DD250 pKa = 3.38 QKK252 pKa = 8.8 VHH254 pKa = 5.41 VSAPVLGLDD263 pKa = 3.13 GWYY266 pKa = 10.56 GYY268 pKa = 9.95 NRR270 pKa = 11.84 EE271 pKa = 3.97 VARR274 pKa = 11.84 AEE276 pKa = 4.04 LNGIRR281 pKa = 11.84 HH282 pKa = 5.84 EE283 pKa = 4.66 LIGMRR288 pKa = 11.84 TNLRR292 pKa = 11.84 MDD294 pKa = 3.38 ITSAIAGDD302 pKa = 3.58 ARR304 pKa = 11.84 QLGFEE309 pKa = 5.15 PISTLYY315 pKa = 11.42 DD316 pKa = 3.44 MVPLSFVSGWVTNFDD331 pKa = 3.47 AYY333 pKa = 11.24 VRR335 pKa = 11.84 TIAPLVGLSFRR346 pKa = 11.84 TGSRR350 pKa = 11.84 NLRR353 pKa = 11.84 KK354 pKa = 9.48 QVVLDD359 pKa = 3.28 VAGRR363 pKa = 11.84 VLEE366 pKa = 4.34 QNPPTYY372 pKa = 9.54 PAPWKK377 pKa = 10.3 AWVEE381 pKa = 3.85 EE382 pKa = 4.05 RR383 pKa = 11.84 DD384 pKa = 3.55 RR385 pKa = 11.84 SVSLLTGNRR394 pKa = 11.84 TRR396 pKa = 11.84 DD397 pKa = 3.39 EE398 pKa = 4.24 RR399 pKa = 11.84 SLVLEE404 pKa = 4.29 LPKK407 pKa = 10.8 ASLHH411 pKa = 5.95 WDD413 pKa = 2.94 VDD415 pKa = 3.73 VGFNEE420 pKa = 3.83 VTAAISLLIQRR431 pKa = 11.84 KK432 pKa = 8.8 LKK434 pKa = 9.89 PLQRR438 pKa = 11.84 AIGIKK443 pKa = 7.13 QFRR446 pKa = 11.84 YY447 pKa = 9.98 RR448 pKa = 11.84 GPKK451 pKa = 9.2 PKK453 pKa = 9.19 WLQEE457 pKa = 3.26 IRR459 pKa = 11.84 YY460 pKa = 8.32 RR461 pKa = 11.84 KK462 pKa = 8.87 PP463 pKa = 2.82
Molecular weight: 52.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.889
IPC2_protein 8.843
IPC_protein 8.829
Toseland 9.502
ProMoST 9.297
Dawson 9.78
Bjellqvist 9.502
Wikipedia 9.955
Rodwell 9.999
Grimsley 9.853
Solomon 9.809
Lehninger 9.765
Nozaki 9.575
DTASelect 9.472
Thurlkill 9.619
EMBOSS 9.94
Sillero 9.706
Patrickios 5.308
IPC_peptide 9.809
IPC2_peptide 8.083
IPC2.peptide.svr19 7.866
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1136
129
544
378.7
42.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.891 ± 0.534
1.32 ± 0.611
5.722 ± 0.388
6.162 ± 0.747
3.609 ± 0.833
6.074 ± 0.504
1.408 ± 0.154
4.665 ± 0.542
4.137 ± 0.482
9.243 ± 0.468
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.025 ± 0.122
3.257 ± 0.24
6.338 ± 0.789
2.377 ± 0.448
9.155 ± 0.719
6.954 ± 1.535
5.81 ± 1.045
7.658 ± 1.257
2.025 ± 0.32
3.169 ± 0.721
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here