Bacillus phage Leo2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Andromedavirus; unclassified Andromedavirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5QTR5|A0A1S5QTR5_9CAUD Uncharacterized protein OS=Bacillus phage Leo2 OX=1815973 GN=LEO2_61 PE=4 SV=1
MM1 pKa = 7.46AKK3 pKa = 9.62HH4 pKa = 6.1WEE6 pKa = 4.5TVCIFAEE13 pKa = 4.63GNDD16 pKa = 3.28YY17 pKa = 11.28DD18 pKa = 4.05AFEE21 pKa = 4.57VEE23 pKa = 4.37VLEE26 pKa = 5.16GFNFTQAPLIEE37 pKa = 4.74EE38 pKa = 4.96YY39 pKa = 11.03YY40 pKa = 11.07NSLDD44 pKa = 4.97SPDD47 pKa = 4.57DD48 pKa = 3.42QDD50 pKa = 5.78KK51 pKa = 11.25FIMDD55 pKa = 3.78NKK57 pKa = 9.95LAYY60 pKa = 9.37IGSGHH65 pKa = 6.22FGEE68 pKa = 5.87VFGLDD73 pKa = 3.2DD74 pKa = 4.06KK75 pKa = 11.66LAIKK79 pKa = 10.14LLHH82 pKa = 6.27NLGEE86 pKa = 4.35EE87 pKa = 4.34LPDD90 pKa = 4.57GYY92 pKa = 10.54ILSQLNHH99 pKa = 6.66SDD101 pKa = 3.25MTPNVYY107 pKa = 10.13AYY109 pKa = 10.48CDD111 pKa = 3.64EE112 pKa = 5.09PDD114 pKa = 3.49VGFMIVDD121 pKa = 4.54RR122 pKa = 11.84IIGMNVCDD130 pKa = 3.24IHH132 pKa = 8.29VNSLLGFDD140 pKa = 5.31LDD142 pKa = 3.92KK143 pKa = 10.46QLKK146 pKa = 10.1RR147 pKa = 11.84IAQFFEE153 pKa = 3.89DD154 pKa = 3.74CRR156 pKa = 11.84RR157 pKa = 11.84AEE159 pKa = 4.06VLPSDD164 pKa = 3.86LHH166 pKa = 5.69QANVMCTYY174 pKa = 10.19EE175 pKa = 5.3GDD177 pKa = 3.72LMIVDD182 pKa = 4.29VGCFEE187 pKa = 4.73YY188 pKa = 11.14GSFDD192 pKa = 4.16PNSIEE197 pKa = 4.36AKK199 pKa = 9.32WYY201 pKa = 9.43YY202 pKa = 10.92EE203 pKa = 3.83NAKK206 pKa = 10.04WEE208 pKa = 4.29MLYY211 pKa = 9.99QAYY214 pKa = 10.58LIDD217 pKa = 5.04HH218 pKa = 7.04IVNGSPMRR226 pKa = 11.84HH227 pKa = 5.64IPDD230 pKa = 5.06YY231 pKa = 10.16IPQMFRR237 pKa = 11.84WEE239 pKa = 4.42SEE241 pKa = 3.94TSGMFDD247 pKa = 2.91HH248 pKa = 7.23HH249 pKa = 6.49VQSFLKK255 pKa = 10.52DD256 pKa = 3.59FRR258 pKa = 11.84DD259 pKa = 3.42HH260 pKa = 7.43DD261 pKa = 4.38KK262 pKa = 11.38VVLPQCQQ269 pKa = 2.73

Molecular weight: 31.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5QTN0|A0A1S5QTN0_9CAUD Uncharacterized protein OS=Bacillus phage Leo2 OX=1815973 GN=LEO2_21 PE=4 SV=1
MM1 pKa = 7.21AQVTVNFNGLDD12 pKa = 3.22ARR14 pKa = 11.84LRR16 pKa = 11.84QIAKK20 pKa = 8.57EE21 pKa = 4.1CQDD24 pKa = 2.78FRR26 pKa = 11.84LPLRR30 pKa = 11.84QSAVYY35 pKa = 10.54LEE37 pKa = 4.59GSISRR42 pKa = 11.84RR43 pKa = 11.84FSQGGGSKK51 pKa = 10.65GKK53 pKa = 9.22WKK55 pKa = 10.46SLSPATIKK63 pKa = 10.43RR64 pKa = 11.84HH65 pKa = 4.35PHH67 pKa = 5.89RR68 pKa = 11.84SGGTPLSDD76 pKa = 3.13TGRR79 pKa = 11.84LRR81 pKa = 11.84SSVSSGAIKK90 pKa = 10.38QYY92 pKa = 8.82TPKK95 pKa = 10.73KK96 pKa = 7.75LTYY99 pKa = 10.39SIGSNVRR106 pKa = 11.84YY107 pKa = 10.01ASVHH111 pKa = 5.17NFGSGRR117 pKa = 11.84IPRR120 pKa = 11.84RR121 pKa = 11.84EE122 pKa = 3.66FMYY125 pKa = 10.46TDD127 pKa = 3.29SKK129 pKa = 11.43DD130 pKa = 3.39EE131 pKa = 4.28RR132 pKa = 11.84EE133 pKa = 4.24INTIFADD140 pKa = 3.96YY141 pKa = 10.17IRR143 pKa = 11.84RR144 pKa = 11.84IAQQ147 pKa = 3.09

Molecular weight: 16.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

14673

41

1024

198.3

22.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.188 ± 0.408

0.913 ± 0.132

6.215 ± 0.257

7.967 ± 0.445

4.546 ± 0.193

6.822 ± 0.318

2.085 ± 0.179

6.679 ± 0.218

7.987 ± 0.446

7.858 ± 0.426

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.665 ± 0.167

4.818 ± 0.323

3.305 ± 0.167

3.694 ± 0.175

4.566 ± 0.243

6.42 ± 0.385

5.827 ± 0.407

6.386 ± 0.252

1.097 ± 0.136

3.96 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski