Amino acid dipepetide frequency for Pseudomonas phage PspYZU05

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.802AlaAla: 3.802 ± 0.361
0.506AlaCys: 0.506 ± 0.092
3.222AlaAsp: 3.222 ± 0.22
4.308AlaGlu: 4.308 ± 0.357
2.042AlaPhe: 2.042 ± 0.196
3.409AlaGly: 3.409 ± 0.329
0.862AlaHis: 0.862 ± 0.177
4.889AlaIle: 4.889 ± 0.357
4.795AlaLys: 4.795 ± 0.343
5.451AlaLeu: 5.451 ± 0.345
1.461AlaMet: 1.461 ± 0.199
3.447AlaAsn: 3.447 ± 0.316
2.192AlaPro: 2.192 ± 0.199
1.761AlaGln: 1.761 ± 0.207
2.547AlaArg: 2.547 ± 0.203
4.402AlaSer: 4.402 ± 0.376
3.484AlaThr: 3.484 ± 0.316
3.559AlaVal: 3.559 ± 0.284
0.693AlaTrp: 0.693 ± 0.113
2.922AlaTyr: 2.922 ± 0.238
0.0AlaXaa: 0.0 ± 0.0
Cys
0.637CysAla: 0.637 ± 0.112
0.169CysCys: 0.169 ± 0.056
0.599CysAsp: 0.599 ± 0.106
0.749CysGlu: 0.749 ± 0.117
0.468CysPhe: 0.468 ± 0.11
0.543CysGly: 0.543 ± 0.112
0.206CysHis: 0.206 ± 0.057
0.955CysIle: 0.955 ± 0.144
0.88CysLys: 0.88 ± 0.132
0.712CysLeu: 0.712 ± 0.139
0.3CysMet: 0.3 ± 0.075
0.581CysAsn: 0.581 ± 0.117
0.356CysPro: 0.356 ± 0.077
0.45CysGln: 0.45 ± 0.102
0.412CysArg: 0.412 ± 0.1
0.993CysSer: 0.993 ± 0.15
0.693CysThr: 0.693 ± 0.114
0.88CysVal: 0.88 ± 0.145
0.187CysTrp: 0.187 ± 0.051
0.581CysTyr: 0.581 ± 0.106
0.0CysXaa: 0.0 ± 0.0
Asp
3.315AspAla: 3.315 ± 0.265
0.487AspCys: 0.487 ± 0.1
2.828AspAsp: 2.828 ± 0.254
3.99AspGlu: 3.99 ± 0.326
3.465AspPhe: 3.465 ± 0.261
4.177AspGly: 4.177 ± 0.261
0.899AspHis: 0.899 ± 0.127
5.057AspIle: 5.057 ± 0.317
4.833AspLys: 4.833 ± 0.365
5.582AspLeu: 5.582 ± 0.342
1.461AspMet: 1.461 ± 0.187
3.465AspAsn: 3.465 ± 0.267
2.697AspPro: 2.697 ± 0.216
1.667AspGln: 1.667 ± 0.149
2.117AspArg: 2.117 ± 0.198
4.439AspSer: 4.439 ± 0.277
3.634AspThr: 3.634 ± 0.224
3.709AspVal: 3.709 ± 0.254
1.068AspTrp: 1.068 ± 0.154
2.753AspTyr: 2.753 ± 0.232
0.0AspXaa: 0.0 ± 0.0
Glu
3.971GluAla: 3.971 ± 0.242
0.993GluCys: 0.993 ± 0.136
3.203GluAsp: 3.203 ± 0.261
4.814GluGlu: 4.814 ± 0.354
3.465GluPhe: 3.465 ± 0.268
3.222GluGly: 3.222 ± 0.263
1.648GluHis: 1.648 ± 0.188
5.413GluIle: 5.413 ± 0.354
4.664GluLys: 4.664 ± 0.382
7.886GluLeu: 7.886 ± 0.433
1.967GluMet: 1.967 ± 0.185
3.39GluAsn: 3.39 ± 0.268
1.573GluPro: 1.573 ± 0.166
2.679GluGln: 2.679 ± 0.216
3.072GluArg: 3.072 ± 0.258
4.945GluSer: 4.945 ± 0.325
3.765GluThr: 3.765 ± 0.281
4.57GluVal: 4.57 ± 0.309
0.693GluTrp: 0.693 ± 0.104
3.615GluTyr: 3.615 ± 0.273
0.0GluXaa: 0.0 ± 0.0
Phe
2.135PheAla: 2.135 ± 0.202
0.468PheCys: 0.468 ± 0.111
3.353PheAsp: 3.353 ± 0.269
2.922PheGlu: 2.922 ± 0.236
1.367PhePhe: 1.367 ± 0.165
2.491PheGly: 2.491 ± 0.23
0.656PheHis: 0.656 ± 0.101
3.109PheIle: 3.109 ± 0.253
3.934PheLys: 3.934 ± 0.283
2.547PheLeu: 2.547 ± 0.231
1.292PheMet: 1.292 ± 0.158
2.978PheAsn: 2.978 ± 0.244
1.292PhePro: 1.292 ± 0.166
1.086PheGln: 1.086 ± 0.163
1.798PheArg: 1.798 ± 0.208
3.447PheSer: 3.447 ± 0.236
2.547PheThr: 2.547 ± 0.2
2.641PheVal: 2.641 ± 0.231
0.506PheTrp: 0.506 ± 0.092
1.854PheTyr: 1.854 ± 0.192
0.0PheXaa: 0.0 ± 0.0
Gly
3.222GlyAla: 3.222 ± 0.272
0.375GlyCys: 0.375 ± 0.08
3.653GlyAsp: 3.653 ± 0.288
3.596GlyGlu: 3.596 ± 0.274
2.66GlyPhe: 2.66 ± 0.243
2.735GlyGly: 2.735 ± 0.382
1.124GlyHis: 1.124 ± 0.152
4.327GlyIle: 4.327 ± 0.276
4.271GlyLys: 4.271 ± 0.305
4.421GlyLeu: 4.421 ± 0.303
1.555GlyMet: 1.555 ± 0.173
3.128GlyAsn: 3.128 ± 0.375
1.255GlyPro: 1.255 ± 0.17
2.192GlyGln: 2.192 ± 0.286
2.341GlyArg: 2.341 ± 0.182
4.533GlySer: 4.533 ± 0.402
3.896GlyThr: 3.896 ± 0.378
3.971GlyVal: 3.971 ± 0.272
0.581GlyTrp: 0.581 ± 0.173
2.491GlyTyr: 2.491 ± 0.197
0.0GlyXaa: 0.0 ± 0.0
His
1.086HisAla: 1.086 ± 0.157
0.225HisCys: 0.225 ± 0.068
1.011HisAsp: 1.011 ± 0.143
1.255HisGlu: 1.255 ± 0.144
0.955HisPhe: 0.955 ± 0.129
1.049HisGly: 1.049 ± 0.15
0.281HisHis: 0.281 ± 0.07
1.386HisIle: 1.386 ± 0.171
1.386HisLys: 1.386 ± 0.161
1.911HisLeu: 1.911 ± 0.185
0.506HisMet: 0.506 ± 0.09
0.955HisAsn: 0.955 ± 0.108
0.712HisPro: 0.712 ± 0.127
0.412HisGln: 0.412 ± 0.089
0.918HisArg: 0.918 ± 0.125
1.48HisSer: 1.48 ± 0.156
0.993HisThr: 0.993 ± 0.141
1.161HisVal: 1.161 ± 0.151
0.244HisTrp: 0.244 ± 0.08
1.105HisTyr: 1.105 ± 0.171
0.0HisXaa: 0.0 ± 0.0
Ile
4.57IleAla: 4.57 ± 0.344
1.236IleCys: 1.236 ± 0.138
5.544IleAsp: 5.544 ± 0.368
5.451IleGlu: 5.451 ± 0.349
2.735IlePhe: 2.735 ± 0.23
3.971IleGly: 3.971 ± 0.34
1.461IleHis: 1.461 ± 0.186
5.095IleIle: 5.095 ± 0.337
6.799IleLys: 6.799 ± 0.401
5.114IleLeu: 5.114 ± 0.362
1.836IleMet: 1.836 ± 0.202
4.739IleAsn: 4.739 ± 0.341
2.66IlePro: 2.66 ± 0.241
2.697IleGln: 2.697 ± 0.211
3.521IleArg: 3.521 ± 0.283
5.301IleSer: 5.301 ± 0.325
4.008IleThr: 4.008 ± 0.259
4.664IleVal: 4.664 ± 0.28
0.562IleTrp: 0.562 ± 0.102
2.547IleTyr: 2.547 ± 0.21
0.0IleXaa: 0.0 ± 0.0
Lys
4.758LysAla: 4.758 ± 0.32
0.899LysCys: 0.899 ± 0.143
5.17LysAsp: 5.17 ± 0.391
5.807LysGlu: 5.807 ± 0.426
3.634LysPhe: 3.634 ± 0.251
3.765LysGly: 3.765 ± 0.28
1.836LysHis: 1.836 ± 0.161
4.87LysIle: 4.87 ± 0.361
4.215LysLys: 4.215 ± 0.339
6.931LysLeu: 6.931 ± 0.363
2.323LysMet: 2.323 ± 0.232
3.596LysAsn: 3.596 ± 0.264
2.772LysPro: 2.772 ± 0.218
2.491LysGln: 2.491 ± 0.224
3.353LysArg: 3.353 ± 0.283
4.87LysSer: 4.87 ± 0.3
4.702LysThr: 4.702 ± 0.284
5.132LysVal: 5.132 ± 0.324
1.086LysTrp: 1.086 ± 0.153
3.465LysTyr: 3.465 ± 0.286
0.0LysXaa: 0.0 ± 0.0
Leu
5.132LeuAla: 5.132 ± 0.375
0.993LeuCys: 0.993 ± 0.153
5.825LeuAsp: 5.825 ± 0.348
6.369LeuGlu: 6.369 ± 0.374
3.353LeuPhe: 3.353 ± 0.24
4.57LeuGly: 4.57 ± 0.281
1.705LeuHis: 1.705 ± 0.154
5.713LeuIle: 5.713 ± 0.333
6.593LeuLys: 6.593 ± 0.365
7.043LeuLeu: 7.043 ± 0.417
1.948LeuMet: 1.948 ± 0.195
5.544LeuAsn: 5.544 ± 0.297
3.166LeuPro: 3.166 ± 0.304
2.547LeuGln: 2.547 ± 0.216
4.252LeuArg: 4.252 ± 0.24
6.387LeuSer: 6.387 ± 0.329
4.57LeuThr: 4.57 ± 0.317
5.114LeuVal: 5.114 ± 0.361
0.693LeuTrp: 0.693 ± 0.108
3.091LeuTyr: 3.091 ± 0.243
0.0LeuXaa: 0.0 ± 0.0
Met
1.798MetAla: 1.798 ± 0.22
0.206MetCys: 0.206 ± 0.062
1.592MetAsp: 1.592 ± 0.169
1.442MetGlu: 1.442 ± 0.156
1.255MetPhe: 1.255 ± 0.163
0.824MetGly: 0.824 ± 0.125
0.468MetHis: 0.468 ± 0.093
1.648MetIle: 1.648 ± 0.148
2.06MetLys: 2.06 ± 0.224
2.21MetLeu: 2.21 ± 0.215
0.599MetMet: 0.599 ± 0.118
1.442MetAsn: 1.442 ± 0.183
0.918MetPro: 0.918 ± 0.112
0.993MetGln: 0.993 ± 0.136
1.049MetArg: 1.049 ± 0.14
2.491MetSer: 2.491 ± 0.239
1.648MetThr: 1.648 ± 0.193
1.592MetVal: 1.592 ± 0.17
0.3MetTrp: 0.3 ± 0.093
1.199MetTyr: 1.199 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
3.615AsnAla: 3.615 ± 0.296
0.562AsnCys: 0.562 ± 0.109
3.166AsnAsp: 3.166 ± 0.265
3.578AsnGlu: 3.578 ± 0.255
2.51AsnPhe: 2.51 ± 0.202
4.383AsnGly: 4.383 ± 0.367
1.236AsnHis: 1.236 ± 0.155
4.87AsnIle: 4.87 ± 0.261
4.421AsnLys: 4.421 ± 0.302
4.702AsnLeu: 4.702 ± 0.313
1.517AsnMet: 1.517 ± 0.191
3.203AsnAsn: 3.203 ± 0.271
2.192AsnPro: 2.192 ± 0.206
1.63AsnGln: 1.63 ± 0.176
2.566AsnArg: 2.566 ± 0.23
3.821AsnSer: 3.821 ± 0.305
3.39AsnThr: 3.39 ± 0.223
3.503AsnVal: 3.503 ± 0.295
0.581AsnTrp: 0.581 ± 0.098
2.06AsnTyr: 2.06 ± 0.217
0.0AsnXaa: 0.0 ± 0.0
Pro
2.154ProAla: 2.154 ± 0.223
0.3ProCys: 0.3 ± 0.084
2.154ProAsp: 2.154 ± 0.194
2.922ProGlu: 2.922 ± 0.274
1.311ProPhe: 1.311 ± 0.171
2.266ProGly: 2.266 ± 0.235
0.674ProHis: 0.674 ± 0.115
2.266ProIle: 2.266 ± 0.211
2.547ProLys: 2.547 ± 0.206
2.585ProLeu: 2.585 ± 0.227
0.656ProMet: 0.656 ± 0.119
2.004ProAsn: 2.004 ± 0.174
0.787ProPro: 0.787 ± 0.137
0.862ProGln: 0.862 ± 0.147
1.255ProArg: 1.255 ± 0.167
2.21ProSer: 2.21 ± 0.234
2.248ProThr: 2.248 ± 0.222
2.772ProVal: 2.772 ± 0.21
0.375ProTrp: 0.375 ± 0.087
1.536ProTyr: 1.536 ± 0.163
0.0ProXaa: 0.0 ± 0.0
Gln
1.967GlnAla: 1.967 ± 0.223
0.375GlnCys: 0.375 ± 0.087
1.817GlnAsp: 1.817 ± 0.221
2.416GlnGlu: 2.416 ± 0.199
1.498GlnPhe: 1.498 ± 0.157
1.667GlnGly: 1.667 ± 0.192
0.412GlnHis: 0.412 ± 0.087
2.304GlnIle: 2.304 ± 0.22
2.023GlnLys: 2.023 ± 0.172
2.753GlnLeu: 2.753 ± 0.223
1.124GlnMet: 1.124 ± 0.137
1.555GlnAsn: 1.555 ± 0.157
0.88GlnPro: 0.88 ± 0.124
1.105GlnGln: 1.105 ± 0.189
1.461GlnArg: 1.461 ± 0.188
1.929GlnSer: 1.929 ± 0.195
1.948GlnThr: 1.948 ± 0.197
2.604GlnVal: 2.604 ± 0.226
0.45GlnTrp: 0.45 ± 0.089
1.892GlnTyr: 1.892 ± 0.191
0.0GlnXaa: 0.0 ± 0.0
Arg
2.735ArgAla: 2.735 ± 0.273
0.543ArgCys: 0.543 ± 0.097
2.66ArgAsp: 2.66 ± 0.226
3.034ArgGlu: 3.034 ± 0.248
1.686ArgPhe: 1.686 ± 0.205
2.304ArgGly: 2.304 ± 0.196
0.88ArgHis: 0.88 ± 0.138
4.158ArgIle: 4.158 ± 0.275
3.278ArgLys: 3.278 ± 0.215
3.653ArgLeu: 3.653 ± 0.269
0.918ArgMet: 0.918 ± 0.151
2.622ArgAsn: 2.622 ± 0.188
1.218ArgPro: 1.218 ± 0.152
1.274ArgGln: 1.274 ± 0.147
1.892ArgArg: 1.892 ± 0.19
3.203ArgSer: 3.203 ± 0.256
2.416ArgThr: 2.416 ± 0.232
2.828ArgVal: 2.828 ± 0.239
0.431ArgTrp: 0.431 ± 0.086
2.135ArgTyr: 2.135 ± 0.232
0.0ArgXaa: 0.0 ± 0.0
Ser
3.934SerAla: 3.934 ± 0.323
0.843SerCys: 0.843 ± 0.161
4.421SerAsp: 4.421 ± 0.255
4.795SerGlu: 4.795 ± 0.308
2.753SerPhe: 2.753 ± 0.232
5.469SerGly: 5.469 ± 0.492
1.161SerHis: 1.161 ± 0.148
5.488SerIle: 5.488 ± 0.384
5.395SerLys: 5.395 ± 0.34
6.144SerLeu: 6.144 ± 0.319
1.761SerMet: 1.761 ± 0.188
4.14SerAsn: 4.14 ± 0.362
2.323SerPro: 2.323 ± 0.217
1.967SerGln: 1.967 ± 0.192
3.934SerArg: 3.934 ± 0.313
4.926SerSer: 4.926 ± 0.365
4.271SerThr: 4.271 ± 0.355
5.263SerVal: 5.263 ± 0.342
0.637SerTrp: 0.637 ± 0.126
2.828SerTyr: 2.828 ± 0.255
0.0SerXaa: 0.0 ± 0.0
Thr
3.596ThrAla: 3.596 ± 0.382
0.674ThrCys: 0.674 ± 0.133
3.353ThrAsp: 3.353 ± 0.232
4.233ThrGlu: 4.233 ± 0.238
2.079ThrPhe: 2.079 ± 0.194
3.353ThrGly: 3.353 ± 0.347
1.236ThrHis: 1.236 ± 0.155
4.327ThrIle: 4.327 ± 0.29
4.477ThrLys: 4.477 ± 0.285
5.114ThrLeu: 5.114 ± 0.39
1.311ThrMet: 1.311 ± 0.161
3.278ThrAsn: 3.278 ± 0.266
2.248ThrPro: 2.248 ± 0.225
2.21ThrGln: 2.21 ± 0.254
2.753ThrArg: 2.753 ± 0.226
3.859ThrSer: 3.859 ± 0.3
3.503ThrThr: 3.503 ± 0.296
4.158ThrVal: 4.158 ± 0.248
0.805ThrTrp: 0.805 ± 0.142
2.023ThrTyr: 2.023 ± 0.177
0.0ThrXaa: 0.0 ± 0.0
Val
3.821ValAla: 3.821 ± 0.305
0.637ValCys: 0.637 ± 0.117
4.346ValAsp: 4.346 ± 0.294
4.57ValGlu: 4.57 ± 0.247
2.735ValPhe: 2.735 ± 0.222
2.96ValGly: 2.96 ± 0.263
1.143ValHis: 1.143 ± 0.14
4.889ValIle: 4.889 ± 0.384
5.432ValLys: 5.432 ± 0.328
5.488ValLeu: 5.488 ± 0.365
1.873ValMet: 1.873 ± 0.16
3.559ValAsn: 3.559 ± 0.262
2.416ValPro: 2.416 ± 0.229
2.454ValGln: 2.454 ± 0.243
2.491ValArg: 2.491 ± 0.243
4.795ValSer: 4.795 ± 0.322
4.008ValThr: 4.008 ± 0.279
4.102ValVal: 4.102 ± 0.298
0.862ValTrp: 0.862 ± 0.158
3.128ValTyr: 3.128 ± 0.237
0.0ValXaa: 0.0 ± 0.0
Trp
0.581TrpAla: 0.581 ± 0.102
0.206TrpCys: 0.206 ± 0.083
0.712TrpAsp: 0.712 ± 0.12
1.03TrpGlu: 1.03 ± 0.178
0.524TrpPhe: 0.524 ± 0.078
0.487TrpGly: 0.487 ± 0.095
0.225TrpHis: 0.225 ± 0.064
0.674TrpIle: 0.674 ± 0.142
0.862TrpLys: 0.862 ± 0.128
0.937TrpLeu: 0.937 ± 0.157
0.318TrpMet: 0.318 ± 0.075
0.618TrpAsn: 0.618 ± 0.097
0.618TrpPro: 0.618 ± 0.11
0.412TrpGln: 0.412 ± 0.077
0.393TrpArg: 0.393 ± 0.08
0.824TrpSer: 0.824 ± 0.138
0.674TrpThr: 0.674 ± 0.1
0.824TrpVal: 0.824 ± 0.155
0.094TrpTrp: 0.094 ± 0.04
0.431TrpTyr: 0.431 ± 0.091
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.922TyrAla: 2.922 ± 0.237
0.656TyrCys: 0.656 ± 0.125
3.016TyrAsp: 3.016 ± 0.247
2.266TyrGlu: 2.266 ± 0.236
1.929TyrPhe: 1.929 ± 0.169
2.679TyrGly: 2.679 ± 0.256
0.918TyrHis: 0.918 ± 0.133
3.072TyrIle: 3.072 ± 0.242
2.81TyrLys: 2.81 ± 0.271
3.428TyrLeu: 3.428 ± 0.242
0.993TyrMet: 0.993 ± 0.127
3.278TyrAsn: 3.278 ± 0.26
1.611TyrPro: 1.611 ± 0.189
1.292TyrGln: 1.292 ± 0.184
1.742TyrArg: 1.742 ± 0.178
3.596TyrSer: 3.596 ± 0.315
2.192TyrThr: 2.192 ± 0.186
2.66TyrVal: 2.66 ± 0.253
0.599TyrTrp: 0.599 ± 0.107
2.098TyrTyr: 2.098 ± 0.244
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 224 proteins (53388 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski