Amino acid dipepetide frequency for Vagococcus acidifermentans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.398AlaAla: 7.398 ± 0.128
0.656AlaCys: 0.656 ± 0.029
5.244AlaAsp: 5.244 ± 0.254
5.438AlaGlu: 5.438 ± 0.101
3.397AlaPhe: 3.397 ± 0.078
5.841AlaGly: 5.841 ± 0.088
1.368AlaHis: 1.368 ± 0.043
5.519AlaIle: 5.519 ± 0.083
4.605AlaLys: 4.605 ± 0.073
7.459AlaLeu: 7.459 ± 0.11
2.068AlaMet: 2.068 ± 0.06
2.981AlaAsn: 2.981 ± 0.069
2.126AlaPro: 2.126 ± 0.05
2.5AlaGln: 2.5 ± 0.054
2.837AlaArg: 2.837 ± 0.058
4.1AlaSer: 4.1 ± 0.07
3.802AlaThr: 3.802 ± 0.071
6.417AlaVal: 6.417 ± 0.108
0.558AlaTrp: 0.558 ± 0.03
2.709AlaTyr: 2.709 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
0.484CysAla: 0.484 ± 0.026
0.1CysCys: 0.1 ± 0.013
0.42CysAsp: 0.42 ± 0.021
0.429CysGlu: 0.429 ± 0.025
0.385CysPhe: 0.385 ± 0.021
0.743CysGly: 0.743 ± 0.035
0.243CysHis: 0.243 ± 0.019
0.447CysIle: 0.447 ± 0.023
0.292CysLys: 0.292 ± 0.021
0.857CysLeu: 0.857 ± 0.036
0.146CysMet: 0.146 ± 0.014
0.231CysAsn: 0.231 ± 0.017
0.325CysPro: 0.325 ± 0.02
0.339CysGln: 0.339 ± 0.021
0.432CysArg: 0.432 ± 0.028
0.486CysSer: 0.486 ± 0.029
0.342CysThr: 0.342 ± 0.024
0.535CysVal: 0.535 ± 0.025
0.089CysTrp: 0.089 ± 0.01
0.324CysTyr: 0.324 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
4.222AspAla: 4.222 ± 0.242
0.432AspCys: 0.432 ± 0.02
3.272AspAsp: 3.272 ± 0.075
4.32AspGlu: 4.32 ± 0.074
2.828AspPhe: 2.828 ± 0.056
3.953AspGly: 3.953 ± 0.081
0.884AspHis: 0.884 ± 0.033
4.738AspIle: 4.738 ± 0.072
3.732AspLys: 3.732 ± 0.069
4.94AspLeu: 4.94 ± 0.078
1.781AspMet: 1.781 ± 0.043
2.33AspAsn: 2.33 ± 0.069
1.664AspPro: 1.664 ± 0.061
1.611AspGln: 1.611 ± 0.046
2.147AspArg: 2.147 ± 0.059
3.123AspSer: 3.123 ± 0.062
3.22AspThr: 3.22 ± 0.075
4.481AspVal: 4.481 ± 0.079
0.691AspTrp: 0.691 ± 0.034
2.627AspTyr: 2.627 ± 0.057
0.0AspXaa: 0.0 ± 0.0
Glu
5.38GluAla: 5.38 ± 0.094
0.357GluCys: 0.357 ± 0.021
3.504GluAsp: 3.504 ± 0.061
5.634GluGlu: 5.634 ± 0.103
2.426GluPhe: 2.426 ± 0.054
3.717GluGly: 3.717 ± 0.078
1.287GluHis: 1.287 ± 0.044
4.744GluIle: 4.744 ± 0.079
5.827GluLys: 5.827 ± 0.104
7.084GluLeu: 7.084 ± 0.101
2.028GluMet: 2.028 ± 0.049
3.266GluAsn: 3.266 ± 0.07
1.901GluPro: 1.901 ± 0.051
3.373GluGln: 3.373 ± 0.076
3.26GluArg: 3.26 ± 0.064
3.268GluSer: 3.268 ± 0.073
4.199GluThr: 4.199 ± 0.084
4.402GluVal: 4.402 ± 0.081
0.6GluTrp: 0.6 ± 0.028
2.065GluTyr: 2.065 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
3.104PheAla: 3.104 ± 0.068
0.397PheCys: 0.397 ± 0.022
2.865PheAsp: 2.865 ± 0.06
2.621PheGlu: 2.621 ± 0.064
2.428PhePhe: 2.428 ± 0.072
3.246PheGly: 3.246 ± 0.075
0.862PheHis: 0.862 ± 0.039
3.255PheIle: 3.255 ± 0.084
2.36PheLys: 2.36 ± 0.055
4.513PheLeu: 4.513 ± 0.09
1.159PheMet: 1.159 ± 0.043
1.987PheAsn: 1.987 ± 0.051
1.671PhePro: 1.671 ± 0.046
1.61PheGln: 1.61 ± 0.048
1.57PheArg: 1.57 ± 0.044
3.523PheSer: 3.523 ± 0.07
2.327PheThr: 2.327 ± 0.058
3.092PheVal: 3.092 ± 0.067
0.418PheTrp: 0.418 ± 0.021
1.791PheTyr: 1.791 ± 0.049
0.0PheXaa: 0.0 ± 0.0
Gly
4.859GlyAla: 4.859 ± 0.097
0.634GlyCys: 0.634 ± 0.031
3.408GlyAsp: 3.408 ± 0.071
4.092GlyGlu: 4.092 ± 0.086
3.133GlyPhe: 3.133 ± 0.067
4.746GlyGly: 4.746 ± 0.099
1.229GlyHis: 1.229 ± 0.042
5.773GlyIle: 5.773 ± 0.111
4.845GlyLys: 4.845 ± 0.092
6.583GlyLeu: 6.583 ± 0.091
2.129GlyMet: 2.129 ± 0.052
2.694GlyAsn: 2.694 ± 0.057
1.517GlyPro: 1.517 ± 0.046
2.5GlyGln: 2.5 ± 0.064
2.807GlyArg: 2.807 ± 0.054
3.822GlySer: 3.822 ± 0.066
4.227GlyThr: 4.227 ± 0.077
4.969GlyVal: 4.969 ± 0.078
0.63GlyTrp: 0.63 ± 0.025
2.862GlyTyr: 2.862 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
1.322HisAla: 1.322 ± 0.041
0.2HisCys: 0.2 ± 0.016
1.138HisAsp: 1.138 ± 0.036
1.179HisGlu: 1.179 ± 0.038
0.998HisPhe: 0.998 ± 0.034
1.367HisGly: 1.367 ± 0.047
0.581HisHis: 0.581 ± 0.031
1.408HisIle: 1.408 ± 0.039
0.857HisLys: 0.857 ± 0.033
2.089HisLeu: 2.089 ± 0.048
0.482HisMet: 0.482 ± 0.026
0.639HisAsn: 0.639 ± 0.03
0.996HisPro: 0.996 ± 0.035
0.96HisGln: 0.96 ± 0.033
0.885HisArg: 0.885 ± 0.033
1.086HisSer: 1.086 ± 0.036
1.081HisThr: 1.081 ± 0.039
1.403HisVal: 1.403 ± 0.041
0.181HisTrp: 0.181 ± 0.016
0.853HisTyr: 0.853 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
6.071IleAla: 6.071 ± 0.096
0.651IleCys: 0.651 ± 0.032
4.529IleAsp: 4.529 ± 0.067
4.687IleGlu: 4.687 ± 0.084
3.092IlePhe: 3.092 ± 0.073
5.436IleGly: 5.436 ± 0.098
1.414IleHis: 1.414 ± 0.043
5.349IleIle: 5.349 ± 0.099
4.052IleLys: 4.052 ± 0.074
6.558IleLeu: 6.558 ± 0.092
1.801IleMet: 1.801 ± 0.046
3.074IleAsn: 3.074 ± 0.071
3.025IlePro: 3.025 ± 0.066
2.703IleGln: 2.703 ± 0.059
3.191IleArg: 3.191 ± 0.06
4.791IleSer: 4.791 ± 0.083
4.048IleThr: 4.048 ± 0.077
5.646IleVal: 5.646 ± 0.092
0.525IleTrp: 0.525 ± 0.027
2.349IleTyr: 2.349 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
4.149LysAla: 4.149 ± 0.081
0.264LysCys: 0.264 ± 0.021
3.467LysAsp: 3.467 ± 0.061
5.954LysGlu: 5.954 ± 0.104
1.764LysPhe: 1.764 ± 0.044
3.7LysGly: 3.7 ± 0.066
1.275LysHis: 1.275 ± 0.04
4.466LysIle: 4.466 ± 0.087
5.71LysLys: 5.71 ± 0.099
5.456LysLeu: 5.456 ± 0.08
1.943LysMet: 1.943 ± 0.044
3.302LysAsn: 3.302 ± 0.067
1.911LysPro: 1.911 ± 0.052
3.321LysGln: 3.321 ± 0.079
3.176LysArg: 3.176 ± 0.066
3.215LysSer: 3.215 ± 0.062
3.635LysThr: 3.635 ± 0.065
3.934LysVal: 3.934 ± 0.079
0.575LysTrp: 0.575 ± 0.028
2.007LysTyr: 2.007 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
8.807LeuAla: 8.807 ± 0.116
0.781LeuCys: 0.781 ± 0.031
5.318LeuAsp: 5.318 ± 0.09
5.802LeuGlu: 5.802 ± 0.102
4.754LeuPhe: 4.754 ± 0.1
6.274LeuGly: 6.274 ± 0.111
1.662LeuHis: 1.662 ± 0.044
7.005LeuIle: 7.005 ± 0.107
6.101LeuLys: 6.101 ± 0.089
10.696LeuLeu: 10.696 ± 0.151
2.698LeuMet: 2.698 ± 0.068
4.217LeuAsn: 4.217 ± 0.08
4.444LeuPro: 4.444 ± 0.085
3.27LeuGln: 3.27 ± 0.071
3.546LeuArg: 3.546 ± 0.078
7.411LeuSer: 7.411 ± 0.092
6.694LeuThr: 6.694 ± 0.08
6.769LeuVal: 6.769 ± 0.098
0.71LeuTrp: 0.71 ± 0.032
3.035LeuTyr: 3.035 ± 0.063
0.0LeuXaa: 0.0 ± 0.0
Met
2.292MetAla: 2.292 ± 0.058
0.171MetCys: 0.171 ± 0.016
1.39MetAsp: 1.39 ± 0.039
1.505MetGlu: 1.505 ± 0.039
0.984MetPhe: 0.984 ± 0.04
1.744MetGly: 1.744 ± 0.046
0.407MetHis: 0.407 ± 0.024
2.186MetIle: 2.186 ± 0.058
2.123MetLys: 2.123 ± 0.05
2.691MetLeu: 2.691 ± 0.065
0.874MetMet: 0.874 ± 0.036
1.333MetAsn: 1.333 ± 0.039
1.005MetPro: 1.005 ± 0.037
0.903MetGln: 0.903 ± 0.035
1.053MetArg: 1.053 ± 0.036
1.824MetSer: 1.824 ± 0.054
2.254MetThr: 2.254 ± 0.058
1.693MetVal: 1.693 ± 0.046
0.165MetTrp: 0.165 ± 0.016
0.75MetTyr: 0.75 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.688AsnAla: 2.688 ± 0.058
0.303AsnCys: 0.303 ± 0.022
2.318AsnAsp: 2.318 ± 0.059
2.76AsnGlu: 2.76 ± 0.065
1.621AsnPhe: 1.621 ± 0.045
3.051AsnGly: 3.051 ± 0.056
0.97AsnHis: 0.97 ± 0.034
3.173AsnIle: 3.173 ± 0.059
2.46AsnLys: 2.46 ± 0.06
3.77AsnLeu: 3.77 ± 0.066
1.228AsnMet: 1.228 ± 0.041
1.931AsnAsn: 1.931 ± 0.054
1.953AsnPro: 1.953 ± 0.045
2.244AsnGln: 2.244 ± 0.058
1.956AsnArg: 1.956 ± 0.05
2.199AsnSer: 2.199 ± 0.067
2.085AsnThr: 2.085 ± 0.054
2.906AsnVal: 2.906 ± 0.063
0.458AsnTrp: 0.458 ± 0.025
1.665AsnTyr: 1.665 ± 0.049
0.0AsnXaa: 0.0 ± 0.0
Pro
2.826ProAla: 2.826 ± 0.066
0.215ProCys: 0.215 ± 0.016
2.318ProAsp: 2.318 ± 0.055
3.24ProGlu: 3.24 ± 0.067
1.79ProPhe: 1.79 ± 0.048
2.065ProGly: 2.065 ± 0.057
0.715ProHis: 0.715 ± 0.031
2.303ProIle: 2.303 ± 0.06
1.965ProLys: 1.965 ± 0.048
3.522ProLeu: 3.522 ± 0.07
0.758ProMet: 0.758 ± 0.034
1.466ProAsn: 1.466 ± 0.047
0.893ProPro: 0.893 ± 0.036
1.304ProGln: 1.304 ± 0.045
1.078ProArg: 1.078 ± 0.037
2.006ProSer: 2.006 ± 0.048
1.953ProThr: 1.953 ± 0.057
3.15ProVal: 3.15 ± 0.071
0.3ProTrp: 0.3 ± 0.021
1.4ProTyr: 1.4 ± 0.045
0.0ProXaa: 0.0 ± 0.0
Gln
3.285GlnAla: 3.285 ± 0.067
0.237GlnCys: 0.237 ± 0.018
1.828GlnAsp: 1.828 ± 0.049
2.943GlnGlu: 2.943 ± 0.071
1.621GlnPhe: 1.621 ± 0.043
2.168GlnGly: 2.168 ± 0.051
0.897GlnHis: 0.897 ± 0.037
2.627GlnIle: 2.627 ± 0.058
2.749GlnLys: 2.749 ± 0.071
4.882GlnLeu: 4.882 ± 0.099
1.118GlnMet: 1.118 ± 0.039
1.449GlnAsn: 1.449 ± 0.044
1.446GlnPro: 1.446 ± 0.044
2.304GlnGln: 2.304 ± 0.071
1.766GlnArg: 1.766 ± 0.057
2.128GlnSer: 2.128 ± 0.049
2.61GlnThr: 2.61 ± 0.059
2.634GlnVal: 2.634 ± 0.06
0.372GlnTrp: 0.372 ± 0.023
1.158GlnTyr: 1.158 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
2.476ArgAla: 2.476 ± 0.055
0.314ArgCys: 0.314 ± 0.02
2.132ArgAsp: 2.132 ± 0.053
3.069ArgGlu: 3.069 ± 0.075
2.0ArgPhe: 2.0 ± 0.053
2.251ArgGly: 2.251 ± 0.061
0.908ArgHis: 0.908 ± 0.031
3.093ArgIle: 3.093 ± 0.068
2.979ArgLys: 2.979 ± 0.065
4.494ArgLeu: 4.494 ± 0.078
1.279ArgMet: 1.279 ± 0.036
1.684ArgAsn: 1.684 ± 0.048
1.415ArgPro: 1.415 ± 0.046
2.147ArgGln: 2.147 ± 0.056
2.185ArgArg: 2.185 ± 0.064
2.146ArgSer: 2.146 ± 0.055
2.262ArgThr: 2.262 ± 0.055
2.742ArgVal: 2.742 ± 0.059
0.356ArgTrp: 0.356 ± 0.025
1.714ArgTyr: 1.714 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
4.234SerAla: 4.234 ± 0.076
0.444SerCys: 0.444 ± 0.025
3.498SerAsp: 3.498 ± 0.072
4.086SerGlu: 4.086 ± 0.079
3.047SerPhe: 3.047 ± 0.065
4.989SerGly: 4.989 ± 0.092
1.279SerHis: 1.279 ± 0.037
4.064SerIle: 4.064 ± 0.074
3.322SerLys: 3.322 ± 0.066
6.167SerLeu: 6.167 ± 0.099
1.426SerMet: 1.426 ± 0.041
2.161SerAsn: 2.161 ± 0.056
2.056SerPro: 2.056 ± 0.052
2.413SerGln: 2.413 ± 0.055
2.691SerArg: 2.691 ± 0.059
3.796SerSer: 3.796 ± 0.093
2.96SerThr: 2.96 ± 0.067
4.379SerVal: 4.379 ± 0.071
0.618SerTrp: 0.618 ± 0.027
2.357SerTyr: 2.357 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
4.786ThrAla: 4.786 ± 0.086
0.426ThrCys: 0.426 ± 0.021
3.576ThrAsp: 3.576 ± 0.069
3.669ThrGlu: 3.669 ± 0.082
2.691ThrPhe: 2.691 ± 0.063
4.571ThrGly: 4.571 ± 0.078
1.232ThrHis: 1.232 ± 0.037
4.551ThrIle: 4.551 ± 0.077
3.035ThrLys: 3.035 ± 0.064
5.83ThrLeu: 5.83 ± 0.082
1.337ThrMet: 1.337 ± 0.039
2.328ThrAsn: 2.328 ± 0.05
2.421ThrPro: 2.421 ± 0.064
1.818ThrGln: 1.818 ± 0.046
2.082ThrArg: 2.082 ± 0.051
3.337ThrSer: 3.337 ± 0.08
3.331ThrThr: 3.331 ± 0.082
4.768ThrVal: 4.768 ± 0.093
0.486ThrTrp: 0.486 ± 0.028
2.159ThrTyr: 2.159 ± 0.054
0.0ThrXaa: 0.0 ± 0.0
Val
5.764ValAla: 5.764 ± 0.097
0.658ValCys: 0.658 ± 0.03
4.014ValAsp: 4.014 ± 0.078
4.072ValGlu: 4.072 ± 0.066
3.438ValPhe: 3.438 ± 0.066
4.478ValGly: 4.478 ± 0.08
1.302ValHis: 1.302 ± 0.042
5.613ValIle: 5.613 ± 0.082
4.121ValLys: 4.121 ± 0.069
7.284ValLeu: 7.284 ± 0.112
2.083ValMet: 2.083 ± 0.049
2.944ValAsn: 2.944 ± 0.067
2.882ValPro: 2.882 ± 0.058
2.279ValGln: 2.279 ± 0.053
2.694ValArg: 2.694 ± 0.068
5.173ValSer: 5.173 ± 0.079
4.89ValThr: 4.89 ± 0.091
5.377ValVal: 5.377 ± 0.103
0.594ValTrp: 0.594 ± 0.022
2.513ValTyr: 2.513 ± 0.061
0.0ValXaa: 0.0 ± 0.0
Trp
0.47TrpAla: 0.47 ± 0.026
0.072TrpCys: 0.072 ± 0.009
0.46TrpAsp: 0.46 ± 0.022
0.583TrpGlu: 0.583 ± 0.029
0.465TrpPhe: 0.465 ± 0.026
0.589TrpGly: 0.589 ± 0.035
0.243TrpHis: 0.243 ± 0.02
0.51TrpIle: 0.51 ± 0.026
0.43TrpLys: 0.43 ± 0.025
1.175TrpLeu: 1.175 ± 0.038
0.221TrpMet: 0.221 ± 0.016
0.347TrpAsn: 0.347 ± 0.023
0.244TrpPro: 0.244 ± 0.018
0.665TrpGln: 0.665 ± 0.033
0.42TrpArg: 0.42 ± 0.024
0.471TrpSer: 0.471 ± 0.027
0.453TrpThr: 0.453 ± 0.025
0.527TrpVal: 0.527 ± 0.025
0.097TrpTrp: 0.097 ± 0.009
0.343TrpTyr: 0.343 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.403TyrAla: 2.403 ± 0.057
0.333TyrCys: 0.333 ± 0.02
2.298TyrAsp: 2.298 ± 0.054
2.242TyrGlu: 2.242 ± 0.055
1.937TyrPhe: 1.937 ± 0.05
2.53TyrGly: 2.53 ± 0.066
0.947TyrHis: 0.947 ± 0.037
2.169TyrIle: 2.169 ± 0.056
1.659TyrLys: 1.659 ± 0.05
3.944TyrLeu: 3.944 ± 0.074
0.787TyrMet: 0.787 ± 0.034
1.376TyrAsn: 1.376 ± 0.039
1.372TyrPro: 1.372 ± 0.041
1.955TyrGln: 1.955 ± 0.051
1.868TyrArg: 1.868 ± 0.052
2.083TyrSer: 2.083 ± 0.054
2.062TyrThr: 2.062 ± 0.062
2.354TyrVal: 2.354 ± 0.054
0.361TyrTrp: 0.361 ± 0.021
1.546TyrTyr: 1.546 ± 0.044
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2675 proteins (828011 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski