Amino acid dipepetide frequency for Bacillus sp. B1-b2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.363AlaAla: 4.363 ± 0.07	0.536AlaCys: 0.536 ± 0.02	2.881AlaAsp: 2.881 ± 0.054	3.925AlaGlu: 3.925 ± 0.054	2.922AlaPhe: 2.922 ± 0.049	4.277AlaGly: 4.277 ± 0.075	1.056AlaHis: 1.056 ± 0.032	5.905AlaIle: 5.905 ± 0.072	4.383AlaLys: 4.383 ± 0.058	6.294AlaLeu: 6.294 ± 0.087	1.715AlaMet: 1.715 ± 0.037	2.843AlaAsn: 2.843 ± 0.042	1.671AlaPro: 1.671 ± 0.039	1.807AlaGln: 1.807 ± 0.035	2.193AlaArg: 2.193 ± 0.044	3.879AlaSer: 3.879 ± 0.062	3.408AlaThr: 3.408 ± 0.067	4.436AlaVal: 4.436 ± 0.061	0.552AlaTrp: 0.552 ± 0.022	2.152AlaTyr: 2.152 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.406CysAla: 0.406 ± 0.021	0.107CysCys: 0.107 ± 0.009	0.343CysAsp: 0.343 ± 0.018	0.455CysGlu: 0.455 ± 0.018	0.366CysPhe: 0.366 ± 0.017	0.592CysGly: 0.592 ± 0.021	0.179CysHis: 0.179 ± 0.011	0.605CysIle: 0.605 ± 0.02	0.392CysLys: 0.392 ± 0.017	0.713CysLeu: 0.713 ± 0.027	0.22CysMet: 0.22 ± 0.013	0.302CysAsn: 0.302 ± 0.015	0.305CysPro: 0.305 ± 0.016	0.239CysGln: 0.239 ± 0.012	0.259CysArg: 0.259 ± 0.015	0.494CysSer: 0.494 ± 0.018	0.369CysThr: 0.369 ± 0.02	0.395CysVal: 0.395 ± 0.016	0.059CysTrp: 0.059 ± 0.006	0.277CysTyr: 0.277 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	2.818AspAla: 2.818 ± 0.049	0.389AspCys: 0.389 ± 0.018	2.182AspAsp: 2.182 ± 0.045	3.798AspGlu: 3.798 ± 0.055	2.502AspPhe: 2.502 ± 0.047	3.176AspGly: 3.176 ± 0.07	1.0AspHis: 1.0 ± 0.027	4.488AspIle: 4.488 ± 0.059	3.412AspLys: 3.412 ± 0.065	4.907AspLeu: 4.907 ± 0.067	1.234AspMet: 1.234 ± 0.033	2.201AspAsn: 2.201 ± 0.048	1.677AspPro: 1.677 ± 0.039	1.797AspGln: 1.797 ± 0.036	1.935AspArg: 1.935 ± 0.038	3.053AspSer: 3.053 ± 0.057	2.619AspThr: 2.619 ± 0.048	3.328AspVal: 3.328 ± 0.043	0.619AspTrp: 0.619 ± 0.024	2.414AspTyr: 2.414 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	4.742GluAla: 4.742 ± 0.074	0.372GluCys: 0.372 ± 0.019	3.95GluAsp: 3.95 ± 0.052	7.867GluGlu: 7.867 ± 0.098	2.604GluPhe: 2.604 ± 0.043	4.179GluGly: 4.179 ± 0.068	1.451GluHis: 1.451 ± 0.034	6.072GluIle: 6.072 ± 0.067	6.93GluLys: 6.93 ± 0.076	7.553GluLeu: 7.553 ± 0.079	2.319GluMet: 2.319 ± 0.04	4.258GluAsn: 4.258 ± 0.068	1.733GluPro: 1.733 ± 0.036	3.239GluGln: 3.239 ± 0.051	3.029GluArg: 3.029 ± 0.054	3.891GluSer: 3.891 ± 0.048	3.708GluThr: 3.708 ± 0.056	5.307GluVal: 5.307 ± 0.064	0.849GluTrp: 0.849 ± 0.026	2.63GluTyr: 2.63 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	2.748PheAla: 2.748 ± 0.048	0.343PheCys: 0.343 ± 0.014	2.193PheAsp: 2.193 ± 0.042	2.709PheGlu: 2.709 ± 0.045	2.437PhePhe: 2.437 ± 0.056	3.047PheGly: 3.047 ± 0.052	1.109PheHis: 1.109 ± 0.031	4.308PheIle: 4.308 ± 0.065	2.551PheLys: 2.551 ± 0.044	4.843PheLeu: 4.843 ± 0.083	1.188PheMet: 1.188 ± 0.03	1.995PheAsn: 1.995 ± 0.039	1.64PhePro: 1.64 ± 0.034	1.764PheGln: 1.764 ± 0.035	1.463PheArg: 1.463 ± 0.036	3.541PheSer: 3.541 ± 0.05	2.69PheThr: 2.69 ± 0.042	2.903PheVal: 2.903 ± 0.05	0.473PheTrp: 0.473 ± 0.019	1.913PheTyr: 1.913 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	4.089GlyAla: 4.089 ± 0.079	0.544GlyCys: 0.544 ± 0.021	2.963GlyAsp: 2.963 ± 0.051	4.385GlyGlu: 4.385 ± 0.059	3.307GlyPhe: 3.307 ± 0.058	4.329GlyGly: 4.329 ± 0.067	1.184GlyHis: 1.184 ± 0.031	6.083GlyIle: 6.083 ± 0.079	4.899GlyLys: 4.899 ± 0.065	6.122GlyLeu: 6.122 ± 0.08	1.997GlyMet: 1.997 ± 0.041	2.881GlyAsn: 2.881 ± 0.057	1.503GlyPro: 1.503 ± 0.051	1.891GlyGln: 1.891 ± 0.033	2.198GlyArg: 2.198 ± 0.042	3.871GlySer: 3.871 ± 0.054	3.731GlyThr: 3.731 ± 0.072	4.591GlyVal: 4.591 ± 0.072	0.725GlyTrp: 0.725 ± 0.027	2.892GlyTyr: 2.892 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.161HisAla: 1.161 ± 0.03	0.176HisCys: 0.176 ± 0.01	0.867HisAsp: 0.867 ± 0.025	1.249HisGlu: 1.249 ± 0.033	1.072HisPhe: 1.072 ± 0.027	1.201HisGly: 1.201 ± 0.032	0.643HisHis: 0.643 ± 0.021	1.606HisIle: 1.606 ± 0.037	1.101HisLys: 1.101 ± 0.036	2.204HisLeu: 2.204 ± 0.041	0.487HisMet: 0.487 ± 0.021	0.826HisAsn: 0.826 ± 0.028	0.932HisPro: 0.932 ± 0.031	0.805HisGln: 0.805 ± 0.024	0.763HisArg: 0.763 ± 0.028	1.326HisSer: 1.326 ± 0.031	1.124HisThr: 1.124 ± 0.027	1.275HisVal: 1.275 ± 0.033	0.223HisTrp: 0.223 ± 0.012	0.919HisTyr: 0.919 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.816IleAla: 5.816 ± 0.075	0.709IleCys: 0.709 ± 0.021	4.449IleAsp: 4.449 ± 0.063	5.965IleGlu: 5.965 ± 0.081	3.591IlePhe: 3.591 ± 0.061	6.183IleGly: 6.183 ± 0.092	1.854IleHis: 1.854 ± 0.035	7.315IleIle: 7.315 ± 0.107	5.506IleLys: 5.506 ± 0.072	8.223IleLeu: 8.223 ± 0.093	2.034IleMet: 2.034 ± 0.042	4.051IleAsn: 4.051 ± 0.054	3.601IlePro: 3.601 ± 0.054	3.195IleGln: 3.195 ± 0.048	2.969IleArg: 2.969 ± 0.05	6.004IleSer: 6.004 ± 0.079	4.949IleThr: 4.949 ± 0.063	5.672IleVal: 5.672 ± 0.063	0.706IleTrp: 0.706 ± 0.023	2.947IleTyr: 2.947 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	4.217LysAla: 4.217 ± 0.057	0.337LysCys: 0.337 ± 0.015	4.149LysAsp: 4.149 ± 0.077	8.132LysGlu: 8.132 ± 0.086	2.018LysPhe: 2.018 ± 0.041	4.528LysGly: 4.528 ± 0.055	1.323LysHis: 1.323 ± 0.033	5.163LysIle: 5.163 ± 0.069	6.575LysLys: 6.575 ± 0.073	6.168LysLeu: 6.168 ± 0.082	2.301LysMet: 2.301 ± 0.043	4.05LysAsn: 4.05 ± 0.063	2.015LysPro: 2.015 ± 0.037	3.173LysGln: 3.173 ± 0.048	3.095LysArg: 3.095 ± 0.057	3.893LysSer: 3.893 ± 0.055	3.608LysThr: 3.608 ± 0.053	5.075LysVal: 5.075 ± 0.08	0.903LysTrp: 0.903 ± 0.027	2.537LysTyr: 2.537 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	6.616LeuAla: 6.616 ± 0.075	0.663LeuCys: 0.663 ± 0.022	4.963LeuAsp: 4.963 ± 0.059	7.39LeuGlu: 7.39 ± 0.087	5.007LeuPhe: 5.007 ± 0.069	6.081LeuGly: 6.081 ± 0.08	1.947LeuHis: 1.947 ± 0.042	7.82LeuIle: 7.82 ± 0.084	6.802LeuLys: 6.802 ± 0.078	10.818LeuLeu: 10.818 ± 0.126	2.471LeuMet: 2.471 ± 0.047	4.745LeuAsn: 4.745 ± 0.066	3.633LeuPro: 3.633 ± 0.049	3.742LeuGln: 3.742 ± 0.058	3.322LeuArg: 3.322 ± 0.055	7.215LeuSer: 7.215 ± 0.072	5.623LeuThr: 5.623 ± 0.074	6.381LeuVal: 6.381 ± 0.066	0.813LeuTrp: 0.813 ± 0.025	3.355LeuTyr: 3.355 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	1.72MetAla: 1.72 ± 0.038	0.132MetCys: 0.132 ± 0.009	1.587MetAsp: 1.587 ± 0.032	2.253MetGlu: 2.253 ± 0.039	1.002MetPhe: 1.002 ± 0.03	1.642MetGly: 1.642 ± 0.039	0.409MetHis: 0.409 ± 0.016	2.243MetIle: 2.243 ± 0.037	2.57MetLys: 2.57 ± 0.05	2.441MetLeu: 2.441 ± 0.042	0.796MetMet: 0.796 ± 0.028	1.665MetAsn: 1.665 ± 0.031	0.903MetPro: 0.903 ± 0.032	0.91MetGln: 0.91 ± 0.028	0.947MetArg: 0.947 ± 0.027	1.599MetSer: 1.599 ± 0.035	1.441MetThr: 1.441 ± 0.031	1.786MetVal: 1.786 ± 0.04	0.182MetTrp: 0.182 ± 0.011	0.785MetTyr: 0.785 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	2.659AsnAla: 2.659 ± 0.054	0.316AsnCys: 0.316 ± 0.017	2.327AsnAsp: 2.327 ± 0.047	3.806AsnGlu: 3.806 ± 0.058	1.807AsnPhe: 1.807 ± 0.038	3.582AsnGly: 3.582 ± 0.065	1.249AsnHis: 1.249 ± 0.03	4.214AsnIle: 4.214 ± 0.071	3.773AsnLys: 3.773 ± 0.053	4.273AsnLeu: 4.273 ± 0.062	1.32AsnMet: 1.32 ± 0.03	2.688AsnAsn: 2.688 ± 0.052	2.126AsnPro: 2.126 ± 0.045	2.264AsnGln: 2.264 ± 0.043	2.183AsnArg: 2.183 ± 0.046	3.01AsnSer: 3.01 ± 0.051	2.574AsnThr: 2.574 ± 0.052	3.127AsnVal: 3.127 ± 0.052	0.632AsnTrp: 0.632 ± 0.024	1.922AsnTyr: 1.922 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	1.742ProAla: 1.742 ± 0.042	0.223ProCys: 0.223 ± 0.014	1.685ProAsp: 1.685 ± 0.037	2.582ProGlu: 2.582 ± 0.047	1.993ProPhe: 1.993 ± 0.045	1.851ProGly: 1.851 ± 0.051	0.665ProHis: 0.665 ± 0.022	3.037ProIle: 3.037 ± 0.048	2.072ProLys: 2.072 ± 0.037	3.298ProLeu: 3.298 ± 0.05	0.762ProMet: 0.762 ± 0.023	1.77ProAsn: 1.77 ± 0.039	0.846ProPro: 0.846 ± 0.028	0.919ProGln: 0.919 ± 0.025	0.916ProArg: 0.916 ± 0.026	2.301ProSer: 2.301 ± 0.048	2.042ProThr: 2.042 ± 0.056	2.374ProVal: 2.374 ± 0.041	0.319ProTrp: 0.319 ± 0.016	1.344ProTyr: 1.344 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	2.371GlnAla: 2.371 ± 0.038	0.19GlnCys: 0.19 ± 0.012	1.679GlnAsp: 1.679 ± 0.036	3.004GlnGlu: 3.004 ± 0.052	1.561GlnPhe: 1.561 ± 0.039	1.944GlnGly: 1.944 ± 0.041	0.717GlnHis: 0.717 ± 0.023	2.734GlnIle: 2.734 ± 0.042	2.827GlnLys: 2.827 ± 0.041	4.02GlnLeu: 4.02 ± 0.051	1.059GlnMet: 1.059 ± 0.03	1.77GlnAsn: 1.77 ± 0.035	1.055GlnPro: 1.055 ± 0.038	1.709GlnGln: 1.709 ± 0.045	1.34GlnArg: 1.34 ± 0.034	2.242GlnSer: 2.242 ± 0.042	1.993GlnThr: 1.993 ± 0.038	2.365GlnVal: 2.365 ± 0.044	0.38GlnTrp: 0.38 ± 0.017	1.512GlnTyr: 1.512 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	2.01ArgAla: 2.01 ± 0.036	0.215ArgCys: 0.215 ± 0.014	1.804ArgAsp: 1.804 ± 0.036	2.988ArgGlu: 2.988 ± 0.047	1.748ArgPhe: 1.748 ± 0.036	1.996ArgGly: 1.996 ± 0.042	0.654ArgHis: 0.654 ± 0.022	3.094ArgIle: 3.094 ± 0.052	3.284ArgLys: 3.284 ± 0.055	3.569ArgLeu: 3.569 ± 0.061	1.181ArgMet: 1.181 ± 0.03	2.054ArgAsn: 2.054 ± 0.038	0.999ArgPro: 0.999 ± 0.027	1.175ArgGln: 1.175 ± 0.032	1.581ArgArg: 1.581 ± 0.039	2.061ArgSer: 2.061 ± 0.041	1.84ArgThr: 1.84 ± 0.034	2.214ArgVal: 2.214 ± 0.046	0.354ArgTrp: 0.354 ± 0.016	1.468ArgTyr: 1.468 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	3.359SerAla: 3.359 ± 0.052	0.425SerCys: 0.425 ± 0.017	2.966SerAsp: 2.966 ± 0.051	4.14SerGlu: 4.14 ± 0.062	3.632SerPhe: 3.632 ± 0.051	4.121SerGly: 4.121 ± 0.061	1.25SerHis: 1.25 ± 0.036	6.336SerIle: 6.336 ± 0.074	4.502SerLys: 4.502 ± 0.056	6.886SerLeu: 6.886 ± 0.073	1.75SerMet: 1.75 ± 0.035	3.291SerAsn: 3.291 ± 0.056	2.058SerPro: 2.058 ± 0.041	2.007SerGln: 2.007 ± 0.038	2.083SerArg: 2.083 ± 0.041	4.611SerSer: 4.611 ± 0.073	3.676SerThr: 3.676 ± 0.055	4.195SerVal: 4.195 ± 0.055	0.618SerTrp: 0.618 ± 0.023	2.626SerTyr: 2.626 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	3.425ThrAla: 3.425 ± 0.054	0.384ThrCys: 0.384 ± 0.018	2.692ThrAsp: 2.692 ± 0.06	3.707ThrGlu: 3.707 ± 0.057	2.749ThrPhe: 2.749 ± 0.044	3.922ThrGly: 3.922 ± 0.085	0.973ThrHis: 0.973 ± 0.028	5.198ThrIle: 5.198 ± 0.064	3.686ThrLys: 3.686 ± 0.051	5.424ThrLeu: 5.424 ± 0.063	1.272ThrMet: 1.272 ± 0.031	2.965ThrAsn: 2.965 ± 0.048	2.104ThrPro: 2.104 ± 0.043	1.509ThrGln: 1.509 ± 0.032	1.731ThrArg: 1.731 ± 0.033	3.577ThrSer: 3.577 ± 0.06	3.151ThrThr: 3.151 ± 0.06	3.941ThrVal: 3.941 ± 0.08	0.529ThrTrp: 0.529 ± 0.023	2.119ThrTyr: 2.119 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	4.385ValAla: 4.385 ± 0.068	0.575ValCys: 0.575 ± 0.023	3.398ValAsp: 3.398 ± 0.049	5.015ValGlu: 5.015 ± 0.059	2.997ValPhe: 2.997 ± 0.048	4.393ValGly: 4.393 ± 0.065	1.233ValHis: 1.233 ± 0.031	5.695ValIle: 5.695 ± 0.062	4.659ValLys: 4.659 ± 0.066	6.532ValLeu: 6.532 ± 0.077	1.649ValMet: 1.649 ± 0.038	3.211ValAsn: 3.211 ± 0.052	2.357ValPro: 2.357 ± 0.039	2.242ValGln: 2.242 ± 0.042	2.285ValArg: 2.285 ± 0.044	4.738ValSer: 4.738 ± 0.057	4.023ValThr: 4.023 ± 0.072	4.581ValVal: 4.581 ± 0.073	0.633ValTrp: 0.633 ± 0.021	2.25ValTyr: 2.25 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.537TrpAla: 0.537 ± 0.02	0.083TrpCys: 0.083 ± 0.008	0.539TrpAsp: 0.539 ± 0.019	0.706TrpGlu: 0.706 ± 0.027	0.503TrpPhe: 0.503 ± 0.021	0.657TrpGly: 0.657 ± 0.025	0.167TrpHis: 0.167 ± 0.012	0.903TrpIle: 0.903 ± 0.029	0.835TrpLys: 0.835 ± 0.029	1.096TrpLeu: 1.096 ± 0.033	0.296TrpMet: 0.296 ± 0.015	0.612TrpAsn: 0.612 ± 0.02	0.205TrpPro: 0.205 ± 0.011	0.351TrpGln: 0.351 ± 0.014	0.395TrpArg: 0.395 ± 0.016	0.654TrpSer: 0.654 ± 0.021	0.501TrpThr: 0.501 ± 0.02	0.599TrpVal: 0.599 ± 0.019	0.133TrpTrp: 0.133 ± 0.012	0.37TrpTyr: 0.37 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.018TyrAla: 2.018 ± 0.035	0.359TyrCys: 0.359 ± 0.016	1.933TyrAsp: 1.933 ± 0.039	2.611TyrGlu: 2.611 ± 0.048	2.039TyrPhe: 2.039 ± 0.039	2.486TyrGly: 2.486 ± 0.046	0.895TyrHis: 0.895 ± 0.027	2.917TyrIle: 2.917 ± 0.05	2.501TyrLys: 2.501 ± 0.049	4.021TyrLeu: 4.021 ± 0.06	0.96TyrMet: 0.96 ± 0.027	1.674TyrAsn: 1.674 ± 0.04	1.379TyrPro: 1.379 ± 0.031	1.733TyrGln: 1.733 ± 0.039	1.602TyrArg: 1.602 ± 0.029	2.572TyrSer: 2.572 ± 0.042	1.987TyrThr: 1.987 ± 0.043	2.306TyrVal: 2.306 ± 0.04	0.44TyrTrp: 0.44 ± 0.019	1.709TyrTyr: 1.709 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4701 proteins (1366001 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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