Xanthomonas virus Xp10
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7Y5G6|Q7Y5G6_9CAUD 50L OS=Xanthomonas virus Xp10 OX=232237 PE=4 SV=1
MM1 pKa = 7.93 IFIIKK6 pKa = 9.98 QYY8 pKa = 9.78 LTAQDD13 pKa = 2.91 QGTIVGYY20 pKa = 11.12 ADD22 pKa = 4.11 TEE24 pKa = 4.35 DD25 pKa = 3.47 EE26 pKa = 4.42 AYY28 pKa = 10.63 SYY30 pKa = 10.95 IEE32 pKa = 4.44 SLPEE36 pKa = 4.02 DD37 pKa = 3.55 EE38 pKa = 4.23 QFEE41 pKa = 4.67 YY42 pKa = 10.8 EE43 pKa = 4.37 VLGIAQVV50 pKa = 3.12
Molecular weight: 5.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.151
IPC2_protein 3.49
IPC_protein 3.274
Toseland 3.147
ProMoST 3.516
Dawson 3.3
Bjellqvist 3.49
Wikipedia 3.261
Rodwell 3.16
Grimsley 3.083
Solomon 3.185
Lehninger 3.134
Nozaki 3.478
DTASelect 3.516
Thurlkill 3.249
EMBOSS 3.274
Sillero 3.427
Patrickios 0.006
IPC_peptide 3.185
IPC2_peptide 3.376
IPC2.peptide.svr19 3.629
Protein with the highest isoelectric point:
>tr|Q7Y5I4|Q7Y5I4_9CAUD 33L OS=Xanthomonas virus Xp10 OX=232237 PE=4 SV=1
MM1 pKa = 6.74 YY2 pKa = 9.4 WPWRR6 pKa = 11.84 ACTLSCGVGSRR17 pKa = 11.84 AHH19 pKa = 7.02 RR20 pKa = 11.84 ILVSSMIVMMATAAPTAVQTTFSRR44 pKa = 11.84 GACATPRR51 pKa = 11.84 SVCTIGTSAMSVVRR65 pKa = 11.84 GLVAAPRR72 pKa = 11.84 CTTPNMSWSTEE83 pKa = 3.74 DD84 pKa = 4.16 LLCKK88 pKa = 9.63 RR89 pKa = 11.84 TNTAPVVVVSQTKK102 pKa = 9.96 KK103 pKa = 9.39 GSAFEE108 pKa = 4.45 TVTNTLVGFSINWCANMIILPMFGMPISGTTAFHH142 pKa = 6.15 MGIIFTAISIARR154 pKa = 11.84 GYY156 pKa = 8.56 VLRR159 pKa = 11.84 RR160 pKa = 11.84 VFNKK164 pKa = 8.22 ITEE167 pKa = 4.24 RR168 pKa = 11.84 SLSKK172 pKa = 10.98
Molecular weight: 18.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.502
IPC_protein 10.101
Toseland 10.555
ProMoST 10.306
Dawson 10.657
Bjellqvist 10.394
Wikipedia 10.847
Rodwell 10.877
Grimsley 10.701
Solomon 10.76
Lehninger 10.73
Nozaki 10.628
DTASelect 10.35
Thurlkill 10.57
EMBOSS 10.95
Sillero 10.613
Patrickios 10.657
IPC_peptide 10.774
IPC2_peptide 9.897
IPC2.peptide.svr19 8.497
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
60
0
60
13372
50
1574
222.9
24.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.045 ± 0.567
0.987 ± 0.17
5.751 ± 0.193
5.34 ± 0.313
2.864 ± 0.164
7.972 ± 0.197
1.855 ± 0.231
4.816 ± 0.181
5.168 ± 0.369
8.578 ± 0.293
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.445 ± 0.228
3.844 ± 0.204
3.911 ± 0.254
4.868 ± 0.273
5.646 ± 0.23
6.88 ± 0.301
6.222 ± 0.406
6.977 ± 0.291
1.6 ± 0.156
3.231 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here