Mucilaginibacter paludis DSM 18603

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Mucilaginibacter; Mucilaginibacter paludis

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6864 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H1YIE7|H1YIE7_9SPHI Lipid A biosynthesis acyltransferase OS=Mucilaginibacter paludis DSM 18603 OX=714943 GN=Mucpa_3461 PE=4 SV=1
MM1 pKa = 7.1KK2 pKa = 10.64QNILEE7 pKa = 4.22LAGDD11 pKa = 4.39FKK13 pKa = 11.38PIGTDD18 pKa = 2.63ILRR21 pKa = 11.84GLKK24 pKa = 10.07GGNIPALTQAQLEE37 pKa = 4.36KK38 pKa = 10.93LFDD41 pKa = 3.78YY42 pKa = 11.1SGLGGFNSDD51 pKa = 2.78QSKK54 pKa = 10.58RR55 pKa = 11.84FTDD58 pKa = 3.94QIQKK62 pKa = 9.04MEE64 pKa = 4.06QTDD67 pKa = 4.29FGAGMLGAMLTTGGAPIKK85 pKa = 9.77LTEE88 pKa = 4.48QIPTQANPNLGNEE101 pKa = 4.22AAGSYY106 pKa = 10.4FPTINTLWLNPGTIDD121 pKa = 3.38VTVNSSLQSAALLDD135 pKa = 4.54TIAHH139 pKa = 6.2EE140 pKa = 5.27LYY142 pKa = 10.52HH143 pKa = 6.97SYY145 pKa = 8.37QTNILGKK152 pKa = 10.1LLGVFDD158 pKa = 4.83PNSVKK163 pKa = 10.59RR164 pKa = 11.84EE165 pKa = 3.87LDD167 pKa = 3.24ADD169 pKa = 3.99LAGAKK174 pKa = 9.3ILYY177 pKa = 10.04EE178 pKa = 3.98YY179 pKa = 10.44DD180 pKa = 3.44VKK182 pKa = 11.31FNTSYY187 pKa = 10.39YY188 pKa = 7.78NQYY191 pKa = 10.06YY192 pKa = 8.53VQEE195 pKa = 4.88HH196 pKa = 5.64YY197 pKa = 11.12SGTYY201 pKa = 7.77WQLGDD206 pKa = 4.22NNGGLYY212 pKa = 10.55GLSNITSPGVTNDD225 pKa = 3.29FGAIFDD231 pKa = 3.63QVVRR235 pKa = 11.84YY236 pKa = 9.94DD237 pKa = 4.5HH238 pKa = 7.21FNLDD242 pKa = 3.33NYY244 pKa = 11.2NYY246 pKa = 10.39LISNFLHH253 pKa = 7.06SFEE256 pKa = 4.92TPNNSYY262 pKa = 11.26NSLSAAGISDD272 pKa = 3.68TDD274 pKa = 5.36DD275 pKa = 3.41INQSAINRR283 pKa = 11.84LLFYY287 pKa = 10.64RR288 pKa = 11.84PSLLPNAPTSYY299 pKa = 9.53GTIHH303 pKa = 7.15SYY305 pKa = 11.77NDD307 pKa = 2.96VTNYY311 pKa = 8.96IQSVLNYY318 pKa = 9.93YY319 pKa = 9.04YY320 pKa = 10.86KK321 pKa = 10.75KK322 pKa = 10.6DD323 pKa = 3.68VNNGNGFGNNATITTSWHH341 pKa = 5.22YY342 pKa = 10.94SQLLGVFQNASGDD355 pKa = 3.71TMGALQIPPQVMQILVDD372 pKa = 3.76ISIEE376 pKa = 4.11SPMEE380 pKa = 3.42ITQQQFLALGAAMQNGVNLPEE401 pKa = 4.17STVGILSPPTLPPWVDD417 pKa = 3.51PSSSSANQQGLNGWYY432 pKa = 9.85YY433 pKa = 11.15DD434 pKa = 3.72SQSNSWISTIGSYY447 pKa = 10.55PITVGGGSGYY457 pKa = 10.69NFGSQDD463 pKa = 3.21PSIIGGGGVSIWYY476 pKa = 9.73NPTTDD481 pKa = 3.1HH482 pKa = 5.84TQAIASGQQPSAGYY496 pKa = 5.66TTLVKK501 pKa = 10.48SDD503 pKa = 3.68DD504 pKa = 3.73AGITKK509 pKa = 10.06AYY511 pKa = 10.17NNAYY515 pKa = 10.12DD516 pKa = 3.98YY517 pKa = 11.74NNNLPLVARR526 pKa = 11.84EE527 pKa = 3.98AAANAAALAYY537 pKa = 10.43QNAEE541 pKa = 4.08HH542 pKa = 6.74NGLSVAQANANASIAAQQAALNTLLYY568 pKa = 10.25YY569 pKa = 10.75GYY571 pKa = 10.79DD572 pKa = 3.57VDD574 pKa = 6.4DD575 pKa = 5.9LGDD578 pKa = 5.6DD579 pKa = 5.3DD580 pKa = 6.76DD581 pKa = 7.45LDD583 pKa = 6.34DD584 pKa = 6.04DD585 pKa = 5.66LDD587 pKa = 5.84DD588 pKa = 5.8IFDD591 pKa = 4.54IDD593 pKa = 6.0DD594 pKa = 5.28DD595 pKa = 6.2DD596 pKa = 5.8ILDD599 pKa = 4.18DD600 pKa = 3.81TTGTVAVSGSGNSGGGTGGSGGGSTGGGGSSGGGSSSGDD639 pKa = 3.29DD640 pKa = 3.31GSYY643 pKa = 11.26DD644 pKa = 3.84RR645 pKa = 11.84DD646 pKa = 3.37EE647 pKa = 5.66DD648 pKa = 4.41DD649 pKa = 5.64PVDD652 pKa = 3.58TSYY655 pKa = 10.18DD656 pKa = 3.24TSYY659 pKa = 11.56DD660 pKa = 3.74DD661 pKa = 5.19PGDD664 pKa = 3.95GGDD667 pKa = 4.42PSGGDD672 pKa = 3.52YY673 pKa = 11.29NGGGDD678 pKa = 3.96SVGTIYY684 pKa = 10.34IDD686 pKa = 3.45FNSYY690 pKa = 11.31DD691 pKa = 3.57NEE693 pKa = 4.21LEE695 pKa = 4.1QGITVANWWW704 pKa = 3.45

Molecular weight: 75.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H1XZP1|H1XZP1_9SPHI NAD-dependent epimerase/dehydratase OS=Mucilaginibacter paludis DSM 18603 OX=714943 GN=Mucpa_3635 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.39KK6 pKa = 9.62RR7 pKa = 11.84KK8 pKa = 6.72RR9 pKa = 11.84HH10 pKa = 5.17KK11 pKa = 10.05MATHH15 pKa = 6.02KK16 pKa = 10.34RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.26NRR25 pKa = 11.84HH26 pKa = 4.71KK27 pKa = 10.87KK28 pKa = 9.36KK29 pKa = 10.77

Molecular weight: 3.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6864

0

6864

2350594

29

3731

342.5

38.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.605 ± 0.028

0.807 ± 0.009

5.383 ± 0.018

5.107 ± 0.031

4.773 ± 0.02

6.709 ± 0.03

1.903 ± 0.014

7.21 ± 0.023

6.849 ± 0.028

9.584 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.224 ± 0.014

5.817 ± 0.028

3.903 ± 0.015

4.033 ± 0.02

3.902 ± 0.016

6.446 ± 0.025

6.006 ± 0.033

6.266 ± 0.02

1.166 ± 0.011

4.307 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski