Murid herpesvirus 1 (strain Smith) (MuHV-1) (Mouse cytomegalovirus)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Betaherpesvirinae; Muromegalovirus; Murid betaherpesvirus 1

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P24909|VIE2_MUHVS Immediate-early protein 2 OS=Murid herpesvirus 1 (strain Smith) OX=10367 GN=IE2 PE=3 SV=1
MM1 pKa = 7.37EE2 pKa = 5.37PAAPSCNMIMIADD15 pKa = 3.82QASVNAHH22 pKa = 5.32GRR24 pKa = 11.84HH25 pKa = 5.59LDD27 pKa = 3.48EE28 pKa = 4.46NRR30 pKa = 11.84VYY32 pKa = 10.74PSDD35 pKa = 3.65KK36 pKa = 10.84VPAHH40 pKa = 5.79VANKK44 pKa = 9.38ILEE47 pKa = 4.64SGTEE51 pKa = 4.03TVRR54 pKa = 11.84CDD56 pKa = 3.37LTLEE60 pKa = 4.51DD61 pKa = 3.93MLGDD65 pKa = 3.92YY66 pKa = 10.52EE67 pKa = 4.94YY68 pKa = 11.12DD69 pKa = 3.87DD70 pKa = 3.83PTEE73 pKa = 4.03EE74 pKa = 4.43EE75 pKa = 4.78KK76 pKa = 10.87ILMDD80 pKa = 5.63RR81 pKa = 11.84IADD84 pKa = 3.53HH85 pKa = 6.57VGNDD89 pKa = 3.48NSDD92 pKa = 3.36MAIKK96 pKa = 9.79HH97 pKa = 5.19AAVRR101 pKa = 11.84SVLLSCKK108 pKa = 9.47IAHH111 pKa = 7.08LMIKK115 pKa = 10.05QNYY118 pKa = 7.79QSAINSATNILCQLANDD135 pKa = 3.06IFEE138 pKa = 4.79RR139 pKa = 11.84IEE141 pKa = 3.89RR142 pKa = 11.84QRR144 pKa = 11.84KK145 pKa = 7.81MIYY148 pKa = 9.69GCFRR152 pKa = 11.84SEE154 pKa = 4.0FDD156 pKa = 3.25NVQLGRR162 pKa = 11.84LMYY165 pKa = 10.99DD166 pKa = 3.69MYY168 pKa = 11.16PHH170 pKa = 7.14FMPTNLGPSEE180 pKa = 3.96KK181 pKa = 9.44RR182 pKa = 11.84VWMSYY187 pKa = 9.45VGEE190 pKa = 4.71AIVAATNIDD199 pKa = 3.43HH200 pKa = 7.3ALDD203 pKa = 4.09EE204 pKa = 4.33RR205 pKa = 11.84AAWAKK210 pKa = 9.43TDD212 pKa = 3.51CSLPGEE218 pKa = 4.97FKK220 pKa = 10.89PEE222 pKa = 3.85LCVLVGAIRR231 pKa = 11.84RR232 pKa = 11.84LHH234 pKa = 7.2DD235 pKa = 4.02PPCYY239 pKa = 9.78TKK241 pKa = 10.67PFLDD245 pKa = 4.35AKK247 pKa = 10.41SQLAVWQQMKK257 pKa = 10.45AIEE260 pKa = 4.33SEE262 pKa = 4.57SVSTHH267 pKa = 3.93VVVVEE272 pKa = 3.74ALKK275 pKa = 10.88LRR277 pKa = 11.84EE278 pKa = 4.06NLAKK282 pKa = 10.34AVQEE286 pKa = 4.18TIAYY290 pKa = 7.65EE291 pKa = 3.52RR292 pKa = 11.84HH293 pKa = 5.14QYY295 pKa = 10.43HH296 pKa = 6.4RR297 pKa = 11.84VCQMMCNNMKK307 pKa = 10.62DD308 pKa = 3.75HH309 pKa = 7.58LEE311 pKa = 4.33TTCMLARR318 pKa = 11.84GRR320 pKa = 11.84TLATLADD327 pKa = 4.06LRR329 pKa = 11.84STRR332 pKa = 11.84YY333 pKa = 9.95NLALFLLSEE342 pKa = 3.89MHH344 pKa = 6.5IFDD347 pKa = 4.66SFTMPRR353 pKa = 11.84IRR355 pKa = 11.84GAMKK359 pKa = 9.74QARR362 pKa = 11.84CMSYY366 pKa = 9.98VEE368 pKa = 4.22RR369 pKa = 11.84TISLAKK375 pKa = 10.1FRR377 pKa = 11.84EE378 pKa = 4.2LADD381 pKa = 3.61RR382 pKa = 11.84VHH384 pKa = 6.21NRR386 pKa = 11.84SAPSPQGVIEE396 pKa = 4.2EE397 pKa = 4.03QQQAGEE403 pKa = 4.48EE404 pKa = 4.2EE405 pKa = 4.16QQQQQEE411 pKa = 4.09IEE413 pKa = 4.51YY414 pKa = 9.66DD415 pKa = 3.49PEE417 pKa = 4.08MPPLEE422 pKa = 4.27RR423 pKa = 11.84EE424 pKa = 4.14EE425 pKa = 4.13EE426 pKa = 4.21QEE428 pKa = 3.95DD429 pKa = 4.0EE430 pKa = 4.42QVEE433 pKa = 4.38EE434 pKa = 4.54EE435 pKa = 4.37PPADD439 pKa = 3.91EE440 pKa = 4.69EE441 pKa = 4.19EE442 pKa = 4.64GGAVGGVTQEE452 pKa = 4.18EE453 pKa = 4.64PAGEE457 pKa = 4.01ATEE460 pKa = 4.04EE461 pKa = 4.19AEE463 pKa = 4.45EE464 pKa = 5.27DD465 pKa = 3.65EE466 pKa = 4.69SQPGPSDD473 pKa = 3.4NQVVPEE479 pKa = 4.13SSEE482 pKa = 4.27TPTPAEE488 pKa = 4.23DD489 pKa = 4.9EE490 pKa = 4.43EE491 pKa = 4.63TQSADD496 pKa = 3.16EE497 pKa = 4.92GEE499 pKa = 4.37SQEE502 pKa = 5.53LEE504 pKa = 4.19GSQQLILSRR513 pKa = 11.84PAAPLTDD520 pKa = 4.0SEE522 pKa = 4.56TDD524 pKa = 3.27SDD526 pKa = 5.25SEE528 pKa = 5.18DD529 pKa = 3.8DD530 pKa = 5.11DD531 pKa = 4.04EE532 pKa = 4.61VTRR535 pKa = 11.84IPVGFSLMTSPVLQPTTRR553 pKa = 11.84SATAAASSGTAPRR566 pKa = 11.84PALKK570 pKa = 9.96RR571 pKa = 11.84QYY573 pKa = 11.74AMVHH577 pKa = 5.0TRR579 pKa = 11.84SKK581 pKa = 10.79SSEE584 pKa = 3.77NQQQPKK590 pKa = 10.07KK591 pKa = 9.94KK592 pKa = 9.98SKK594 pKa = 10.33KK595 pKa = 9.54

Molecular weight: 66.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q83207|UL33_MUHVS G-protein coupled receptor homolog M33 OS=Murid herpesvirus 1 (strain Smith) OX=10367 PE=3 SV=1
MM1 pKa = 7.91DD2 pKa = 3.75VLLGRR7 pKa = 11.84DD8 pKa = 3.39EE9 pKa = 4.59TMDD12 pKa = 3.42EE13 pKa = 3.98SDD15 pKa = 3.79YY16 pKa = 11.53LHH18 pKa = 6.87VNNTCAPSLGLSIARR33 pKa = 11.84DD34 pKa = 3.42AEE36 pKa = 4.35TAINTVIILIGGPMNFVVLTTQLLSNRR63 pKa = 11.84IYY65 pKa = 10.6RR66 pKa = 11.84SSAPTLYY73 pKa = 7.84MTNLYY78 pKa = 9.43FANLLTVTMLPFLILSNRR96 pKa = 11.84GQISSSPEE104 pKa = 3.56GCKK107 pKa = 10.08LVAVTYY113 pKa = 9.57YY114 pKa = 10.86ASCTAGFSTLALISVNRR131 pKa = 11.84YY132 pKa = 8.7RR133 pKa = 11.84VIHH136 pKa = 6.05QSTNKK141 pKa = 9.66NAAGSKK147 pKa = 9.33KK148 pKa = 8.96KK149 pKa = 9.31TYY151 pKa = 10.44GVLALVWLTSLMCASPAPTYY171 pKa = 9.89VTVLAHH177 pKa = 7.55DD178 pKa = 4.55GDD180 pKa = 4.55TPDD183 pKa = 4.62SVHH186 pKa = 5.51EE187 pKa = 4.01TCIIFFNYY195 pKa = 10.2DD196 pKa = 3.04QVKK199 pKa = 8.12TVLATFKK206 pKa = 10.77ILICIVWGVMPVVMMTWFYY225 pKa = 11.29LFFYY229 pKa = 10.42KK230 pKa = 10.33RR231 pKa = 11.84LKK233 pKa = 8.24LTSYY237 pKa = 10.31RR238 pKa = 11.84RR239 pKa = 11.84RR240 pKa = 11.84SHH242 pKa = 5.87TLAFVSTLILSFLVLQTPFVGIMIFDD268 pKa = 4.24SYY270 pKa = 11.94AVIEE274 pKa = 4.1WDD276 pKa = 3.93VTCEE280 pKa = 4.3SINSRR285 pKa = 11.84DD286 pKa = 3.63AVAMLARR293 pKa = 11.84VVPNFHH299 pKa = 7.08CMLNPVLYY307 pKa = 10.44AFLGRR312 pKa = 11.84DD313 pKa = 3.42FNKK316 pKa = 10.33RR317 pKa = 11.84FMQCITGKK325 pKa = 9.9LFSRR329 pKa = 11.84RR330 pKa = 11.84RR331 pKa = 11.84MLQEE335 pKa = 3.52RR336 pKa = 11.84AGVRR340 pKa = 11.84SPSTPHH346 pKa = 6.15RR347 pKa = 11.84AARR350 pKa = 11.84QLAKK354 pKa = 10.26IGTLTRR360 pKa = 11.84SCSRR364 pKa = 11.84SEE366 pKa = 4.07LQRR369 pKa = 11.84SASAPPPQQ377 pKa = 4.54

Molecular weight: 42.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

10487

139

1191

699.1

77.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.153 ± 0.67

2.393 ± 0.135

6.255 ± 0.263

6.007 ± 0.473

3.986 ± 0.355

6.17 ± 0.411

2.327 ± 0.249

4.262 ± 0.216

4.1 ± 0.337

8.592 ± 0.493

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.784 ± 0.198

3.805 ± 0.188

5.493 ± 0.428

3.042 ± 0.237

7.257 ± 0.417

8.191 ± 0.517

5.874 ± 0.465

7.123 ± 0.497

0.687 ± 0.079

3.5 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski