Streptomyces sp. WM6378

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7484 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M8SSC0|A0A0M8SSC0_9ACTN Acetolactate synthase 1 catalytic subunit (Fragment) OS=Streptomyces sp. WM6378 OX=1415557 GN=ADK54_16225 PE=4 SV=1
MM1 pKa = 7.09LTEE4 pKa = 3.79TTYY7 pKa = 11.5RR8 pKa = 11.84LLGTDD13 pKa = 3.39ALEE16 pKa = 4.17VTDD19 pKa = 5.33FDD21 pKa = 6.02RR22 pKa = 11.84IEE24 pKa = 4.64CGQCGKK30 pKa = 10.75LSAFLDD36 pKa = 4.32DD37 pKa = 5.04ADD39 pKa = 4.39EE40 pKa = 4.71GTDD43 pKa = 3.45AAHH46 pKa = 7.14DD47 pKa = 4.63AGWHH51 pKa = 5.82VGDD54 pKa = 4.86EE55 pKa = 4.75LDD57 pKa = 5.37DD58 pKa = 4.59CGCPHH63 pKa = 6.74QMCPDD68 pKa = 4.56CADD71 pKa = 3.49DD72 pKa = 3.43WCGVRR77 pKa = 11.84DD78 pKa = 3.7TTPRR82 pKa = 11.84LFGMRR87 pKa = 11.84VVV89 pKa = 3.63

Molecular weight: 9.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M8TCV0|A0A0M8TCV0_9ACTN Immediate-early protein 2 OS=Streptomyces sp. WM6378 OX=1415557 GN=ADK54_04825 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7484

0

7484

2339543

21

9501

312.6

33.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.67 ± 0.044

0.822 ± 0.009

5.807 ± 0.023

5.532 ± 0.032

2.817 ± 0.017

9.358 ± 0.028

2.33 ± 0.015

3.337 ± 0.022

2.342 ± 0.024

10.442 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.8 ± 0.014

1.873 ± 0.017

6.013 ± 0.028

2.932 ± 0.017

7.702 ± 0.032

5.199 ± 0.019

6.02 ± 0.027

8.326 ± 0.026

1.544 ± 0.013

2.134 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski