Wuhan insect virus 17

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEV4|A0A1L3KEV4_9VIRU RNA-directed RNA polymerase OS=Wuhan insect virus 17 OX=1923721 PE=4 SV=1
MM1 pKa = 8.09DD2 pKa = 6.11PLDD5 pKa = 4.34CGIMAGVAEE14 pKa = 4.83APVDD18 pKa = 3.86VQRR21 pKa = 11.84AVADD25 pKa = 3.98ALAQAIAAHH34 pKa = 5.21QLRR37 pKa = 11.84YY38 pKa = 9.56PGVLAAVAEE47 pKa = 4.34IGGCVGGVVKK57 pKa = 10.42AAAKK61 pKa = 10.05AVGGAVLSMRR71 pKa = 11.84SPTLVEE77 pKa = 4.31AGLAVALVGVGAKK90 pKa = 10.19AYY92 pKa = 10.63VPALKK97 pKa = 10.32KK98 pKa = 10.18RR99 pKa = 11.84VRR101 pKa = 11.84GWRR104 pKa = 11.84KK105 pKa = 8.08PAVKK109 pKa = 9.9EE110 pKa = 4.19PEE112 pKa = 4.29PEE114 pKa = 3.88QPPAIVPPPEE124 pKa = 3.68IAEE127 pKa = 4.3LLRR130 pKa = 11.84EE131 pKa = 4.14EE132 pKa = 4.59YY133 pKa = 10.94VPEE136 pKa = 4.05STVAGSEE143 pKa = 4.47FIPMEE148 pKa = 4.47RR149 pKa = 11.84PTHH152 pKa = 4.58QVCFGIHH159 pKa = 5.22EE160 pKa = 4.27WDD162 pKa = 3.4AKK164 pKa = 10.45LGRR167 pKa = 11.84LGFKK171 pKa = 10.84VMGAGCLVKK180 pKa = 8.88WTLGANGSAHH190 pKa = 6.44YY191 pKa = 9.88YY192 pKa = 10.12VIVHH196 pKa = 6.29EE197 pKa = 4.33HH198 pKa = 5.6TLVHH202 pKa = 6.18SSHH205 pKa = 5.98MVGHH209 pKa = 6.01NRR211 pKa = 11.84YY212 pKa = 8.19PLDD215 pKa = 3.57YY216 pKa = 10.34KK217 pKa = 11.13VEE219 pKa = 4.0LMRR222 pKa = 11.84NSLQHH227 pKa = 7.1LDD229 pKa = 4.28LKK231 pKa = 10.74CLPATVFHH239 pKa = 6.64QDD241 pKa = 2.59HH242 pKa = 6.04VAIEE246 pKa = 4.23LTQSQVSQLGLAPVRR261 pKa = 11.84RR262 pKa = 11.84IGVLDD267 pKa = 3.34KK268 pKa = 9.56RR269 pKa = 11.84TSVAIVGPNGLGSSGDD285 pKa = 3.48AEE287 pKa = 4.49MISDD291 pKa = 4.01GSIGQMSYY299 pKa = 9.85TGSTIGGFSGCAYY312 pKa = 10.18VSGSAVFGIHH322 pKa = 5.33QAGGARR328 pKa = 11.84NTGYY332 pKa = 8.69TLSYY336 pKa = 10.49LLKK339 pKa = 10.81KK340 pKa = 10.79LMLEE344 pKa = 4.15LGFCKK349 pKa = 9.96RR350 pKa = 11.84ARR352 pKa = 11.84VEE354 pKa = 3.75RR355 pKa = 11.84VAIYY359 pKa = 10.32VCGHH363 pKa = 6.64PVYY366 pKa = 10.24TPPCGDD372 pKa = 3.3LSVPEE377 pKa = 5.26SSDD380 pKa = 3.42QYY382 pKa = 11.58FKK384 pKa = 11.21DD385 pKa = 3.4LYY387 pKa = 9.48MEE389 pKa = 4.5KK390 pKa = 10.54VPIKK394 pKa = 9.71WEE396 pKa = 3.76TWGVDD401 pKa = 3.16EE402 pKa = 5.02VLIEE406 pKa = 3.78IRR408 pKa = 11.84GRR410 pKa = 11.84TSVHH414 pKa = 6.56DD415 pKa = 3.71VGTLRR420 pKa = 11.84RR421 pKa = 11.84KK422 pKa = 10.08FGRR425 pKa = 11.84AWLEE429 pKa = 3.89NLSKK433 pKa = 10.72PVGQRR438 pKa = 11.84HH439 pKa = 5.81ARR441 pKa = 11.84DD442 pKa = 3.59FDD444 pKa = 4.37DD445 pKa = 6.38SIPEE449 pKa = 4.01CAAEE453 pKa = 3.98PLEE456 pKa = 4.64IPSQSGEE463 pKa = 4.12SRR465 pKa = 11.84SPGALSEE472 pKa = 4.46SASSQGVQACQLAVLTATFATLSPEE497 pKa = 3.94LKK499 pKa = 10.54AAFLTQCAQSMTSCSSPVRR518 pKa = 11.84QEE520 pKa = 4.01TGSTQAA526 pKa = 4.29

Molecular weight: 56.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEV4|A0A1L3KEV4_9VIRU RNA-directed RNA polymerase OS=Wuhan insect virus 17 OX=1923721 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84SVDD5 pKa = 4.46DD6 pKa = 4.11EE7 pKa = 4.78LLFPSEE13 pKa = 4.36AGDD16 pKa = 3.69RR17 pKa = 11.84IYY19 pKa = 10.72PGLDD23 pKa = 2.63ARR25 pKa = 11.84QRR27 pKa = 11.84AARR30 pKa = 11.84MEE32 pKa = 4.04RR33 pKa = 11.84EE34 pKa = 4.09SFGVHH39 pKa = 4.26TRR41 pKa = 11.84EE42 pKa = 3.87FAQPASAHH50 pKa = 6.48PEE52 pKa = 3.83PSSEE56 pKa = 3.72ARR58 pKa = 11.84EE59 pKa = 4.1VVLAMLEE66 pKa = 4.21QEE68 pKa = 4.74FDD70 pKa = 3.4PSQWQLPDD78 pKa = 3.45DD79 pKa = 3.85WDD81 pKa = 3.92SFDD84 pKa = 3.33SYY86 pKa = 11.63EE87 pKa = 4.44RR88 pKa = 11.84VLSRR92 pKa = 11.84LNMQSSPGYY101 pKa = 8.77PYY103 pKa = 10.93SSQAPTNGEE112 pKa = 3.77FLGWDD117 pKa = 3.26GVEE120 pKa = 4.06CQNRR124 pKa = 11.84QRR126 pKa = 11.84VMILWRR132 pKa = 11.84DD133 pKa = 3.48VKK135 pKa = 10.82RR136 pKa = 11.84LFEE139 pKa = 4.21EE140 pKa = 4.25EE141 pKa = 4.01QVEE144 pKa = 4.74VILTTFVKK152 pKa = 10.58EE153 pKa = 4.23EE154 pKa = 3.73PHH156 pKa = 5.3TLKK159 pKa = 10.86KK160 pKa = 10.4VGARR164 pKa = 11.84RR165 pKa = 11.84WRR167 pKa = 11.84LIQAAPLHH175 pKa = 5.37VQVWWQLLFCYY186 pKa = 10.55GNDD189 pKa = 2.96AMVRR193 pKa = 11.84EE194 pKa = 4.99SINIPAQQGFTIVGGAWKK212 pKa = 10.2SYY214 pKa = 10.15LDD216 pKa = 3.31SWRR219 pKa = 11.84NKK221 pKa = 9.04GLRR224 pKa = 11.84CGLDD228 pKa = 3.75FSAFDD233 pKa = 3.23WTVRR237 pKa = 11.84KK238 pKa = 9.2WMLDD242 pKa = 3.08DD243 pKa = 4.4ALRR246 pKa = 11.84LRR248 pKa = 11.84LALGRR253 pKa = 11.84GRR255 pKa = 11.84RR256 pKa = 11.84LGDD259 pKa = 2.82WHH261 pKa = 6.0RR262 pKa = 11.84QARR265 pKa = 11.84KK266 pKa = 9.31AYY268 pKa = 8.4HH269 pKa = 6.24AMYY272 pKa = 10.53VNPKK276 pKa = 9.84VLFSDD281 pKa = 4.46GSLWEE286 pKa = 3.88QTVPGIQKK294 pKa = 10.0SGVVNTIADD303 pKa = 3.49NSVIGRR309 pKa = 11.84MNYY312 pKa = 8.38LHH314 pKa = 7.11AWLVYY319 pKa = 9.88LAEE322 pKa = 4.82AEE324 pKa = 4.27QVDD327 pKa = 3.88PEE329 pKa = 4.72LYY331 pKa = 9.54QRR333 pKa = 11.84PASVHH338 pKa = 6.53CGDD341 pKa = 5.02DD342 pKa = 3.24MLVASKK348 pKa = 11.12AKK350 pKa = 10.1LLKK353 pKa = 10.17PYY355 pKa = 10.41LSRR358 pKa = 11.84FGLVVKK364 pKa = 10.51YY365 pKa = 8.01LTQGLEE371 pKa = 4.09FCGHH375 pKa = 6.82KK376 pKa = 10.2FRR378 pKa = 11.84SAGMIPMYY386 pKa = 7.51WTKK389 pKa = 9.99HH390 pKa = 4.32VKK392 pKa = 9.67MMMHH396 pKa = 6.47ATDD399 pKa = 3.53VTSSLDD405 pKa = 3.15AMLRR409 pKa = 11.84MYY411 pKa = 10.08AHH413 pKa = 6.73CTKK416 pKa = 10.36KK417 pKa = 9.35WKK419 pKa = 9.68EE420 pKa = 3.55WAALADD426 pKa = 3.73EE427 pKa = 5.17LGVKK431 pKa = 10.2RR432 pKa = 11.84FSVLLYY438 pKa = 9.68RR439 pKa = 11.84HH440 pKa = 5.98WKK442 pKa = 9.75DD443 pKa = 5.4RR444 pKa = 11.84PADD447 pKa = 3.63WSPAAQTLWW456 pKa = 3.28

Molecular weight: 52.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

982

456

526

491.0

54.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.776 ± 0.816

1.935 ± 0.413

4.786 ± 0.903

6.11 ± 0.126

2.953 ± 0.517

7.943 ± 1.348

2.953 ± 0.068

3.259 ± 0.564

4.481 ± 0.229

9.369 ± 0.48

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.953 ± 0.517

1.629 ± 0.376

5.397 ± 0.528

4.481 ± 0.229

6.415 ± 0.987

7.23 ± 0.434

3.87 ± 0.387

8.656 ± 0.946

2.648 ± 1.159

3.157 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski