Changjiang sobemo-like virus 2
Average proteome isoelectric point is 7.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KEG8|A0A1L3KEG8_9VIRU Capsid protein OS=Changjiang sobemo-like virus 2 OX=1922801 PE=3 SV=1
MM1 pKa = 7.6 IVPKK5 pKa = 10.85 NKK7 pKa = 9.32 FCQSDD12 pKa = 3.71 DD13 pKa = 3.41 YY14 pKa = 11.57 DD15 pKa = 3.58 YY16 pKa = 11.52 CSRR19 pKa = 11.84 DD20 pKa = 3.46 KK21 pKa = 11.17 CWVCLIPLRR30 pKa = 11.84 CSDD33 pKa = 5.26 LIEE36 pKa = 5.01 KK37 pKa = 9.66 YY38 pKa = 10.14 ISYY41 pKa = 10.61 DD42 pKa = 3.08 PYY44 pKa = 10.38 EE45 pKa = 4.56 AFGSEE50 pKa = 4.47 YY51 pKa = 10.62 EE52 pKa = 4.63 SIVLPCEE59 pKa = 3.58 RR60 pKa = 11.84 LAALCYY66 pKa = 10.45 YY67 pKa = 9.99 EE68 pKa = 5.11 SLWTEE73 pKa = 3.9 VRR75 pKa = 11.84 RR76 pKa = 11.84 SSTEE80 pKa = 3.24 WSIARR85 pKa = 11.84 WYY87 pKa = 8.62 STAVDD92 pKa = 3.21 HH93 pKa = 7.26 RR94 pKa = 11.84 GRR96 pKa = 11.84 PRR98 pKa = 11.84 AFRR101 pKa = 11.84 IVGTNLHH108 pKa = 6.77 DD109 pKa = 4.27 LAGVAAMPYY118 pKa = 8.65 PPTMSEE124 pKa = 3.58 MEE126 pKa = 4.62 YY127 pKa = 10.2 ILKK130 pKa = 9.81 EE131 pKa = 4.21 VPHH134 pKa = 7.29 DD135 pKa = 4.98 DD136 pKa = 3.7 IPGEE140 pKa = 4.08 VFKK143 pKa = 11.39 YY144 pKa = 10.49 LGGRR148 pKa = 11.84 TKK150 pKa = 11.02 LSFTLKK156 pKa = 10.05 LFLMFGVLSGTIFSVEE172 pKa = 4.05 ASDD175 pKa = 5.17 QYY177 pKa = 11.61 TSDD180 pKa = 3.43 LVNTGEE186 pKa = 4.23 LARR189 pKa = 11.84 EE190 pKa = 3.78 ITLWPVNEE198 pKa = 4.15 SFRR201 pKa = 11.84 MLEE204 pKa = 3.87 HH205 pKa = 5.76 TTISTTQCHH214 pKa = 5.8 YY215 pKa = 11.48 EE216 pKa = 3.85 NGTCVEE222 pKa = 4.23 YY223 pKa = 10.41 PNLVNQVFDD232 pKa = 3.62 WLRR235 pKa = 11.84 VRR237 pKa = 11.84 VRR239 pKa = 11.84 NLCYY243 pKa = 10.63 LLDD246 pKa = 4.23 EE247 pKa = 4.54 STLLARR253 pKa = 11.84 HH254 pKa = 5.95 SSSVAKK260 pKa = 10.26 EE261 pKa = 3.37 RR262 pKa = 11.84 TRR264 pKa = 11.84 FYY266 pKa = 11.47 KK267 pKa = 10.71 NIIVKK272 pKa = 10.22 DD273 pKa = 3.79 DD274 pKa = 3.66 YY275 pKa = 11.47 FRR277 pKa = 11.84 AEE279 pKa = 4.09 YY280 pKa = 10.89 DD281 pKa = 4.21 DD282 pKa = 5.07 ISTAEE287 pKa = 3.89 NEE289 pKa = 4.75 LYY291 pKa = 11.03 NFMMLFLSFLYY302 pKa = 10.42 GINSGFWSLCILFINFLYY320 pKa = 10.41 FMRR323 pKa = 11.84 FAHH326 pKa = 6.02 NFVVSTPLGFWIYY339 pKa = 10.54 TKK341 pKa = 10.19 VVWVLGIWLGYY352 pKa = 8.78 YY353 pKa = 8.12 VTYY356 pKa = 10.15 HH357 pKa = 6.59 ALVVLLAASISYY369 pKa = 9.23 LHH371 pKa = 6.72 SKK373 pKa = 9.63 MIEE376 pKa = 3.94 LAVVVVMAPYY386 pKa = 9.94 HH387 pKa = 4.78 IWSRR391 pKa = 11.84 FRR393 pKa = 11.84 RR394 pKa = 11.84 TGVKK398 pKa = 10.04 GFVRR402 pKa = 11.84 EE403 pKa = 4.13 MILPGSKK410 pKa = 8.86 IEE412 pKa = 4.16 KK413 pKa = 10.2 FIGAKK418 pKa = 9.31 QPAVAALVVEE428 pKa = 4.94 VDD430 pKa = 4.22 GKK432 pKa = 7.88 WHH434 pKa = 5.8 YY435 pKa = 11.43 SAMCFRR441 pKa = 11.84 YY442 pKa = 9.86 EE443 pKa = 3.85 NHH445 pKa = 6.48 LVSAFHH451 pKa = 7.01 PFAKK455 pKa = 10.12 HH456 pKa = 4.43 MSVGRR461 pKa = 11.84 RR462 pKa = 11.84 MGVAPNLLCKK472 pKa = 10.33 KK473 pKa = 9.83 RR474 pKa = 11.84 WTHH477 pKa = 6.81 DD478 pKa = 3.32 FGAIQVIDD486 pKa = 3.97 PSNYY490 pKa = 7.98 IVDD493 pKa = 4.53 FEE495 pKa = 4.34 RR496 pKa = 11.84 HH497 pKa = 5.58 DD498 pKa = 3.7 VFMLHH503 pKa = 6.27 MSGEE507 pKa = 3.78 FWDD510 pKa = 4.01 RR511 pKa = 11.84 VRR513 pKa = 11.84 LPEE516 pKa = 4.18 PKK518 pKa = 10.07 KK519 pKa = 10.74 SVSNSFNDD527 pKa = 3.32 SATVFSYY534 pKa = 10.17 MAGTLHH540 pKa = 6.6 TSNGVLTSHH549 pKa = 6.78 TNVDD553 pKa = 3.66 YY554 pKa = 10.68 PPNNFMAHH562 pKa = 5.43 TCSTLPGFSGTPVFAANGAVRR583 pKa = 11.84 GVHH586 pKa = 6.11 IGSDD590 pKa = 3.33 NYY592 pKa = 10.31 EE593 pKa = 3.83 EE594 pKa = 4.36 RR595 pKa = 11.84 NIMFRR600 pKa = 11.84 IDD602 pKa = 3.45 HH603 pKa = 6.32 IKK605 pKa = 10.93 YY606 pKa = 9.55 YY607 pKa = 10.76 LNKK610 pKa = 10.43 NKK612 pKa = 8.07 VTRR615 pKa = 11.84 EE616 pKa = 3.98 DD617 pKa = 3.35 SSDD620 pKa = 3.56 FQKK623 pKa = 11.08 KK624 pKa = 7.11 VLRR627 pKa = 11.84 SMKK630 pKa = 9.64 RR631 pKa = 11.84 RR632 pKa = 11.84 KK633 pKa = 9.86 GKK635 pKa = 9.17 EE636 pKa = 3.24 AVYY639 pKa = 10.9 YY640 pKa = 10.73 FDD642 pKa = 6.44 DD643 pKa = 4.25 FDD645 pKa = 4.56 EE646 pKa = 5.43 DD647 pKa = 3.56 NSVAYY652 pKa = 9.86 DD653 pKa = 3.22 IRR655 pKa = 11.84 TGQIEE660 pKa = 4.27 RR661 pKa = 11.84 VPRR664 pKa = 11.84 TEE666 pKa = 4.36 EE667 pKa = 3.54 EE668 pKa = 4.67 DD669 pKa = 3.6 EE670 pKa = 4.32 EE671 pKa = 4.37 FWRR674 pKa = 11.84 VEE676 pKa = 3.71 MQKK679 pKa = 10.5 PVGLWGDD686 pKa = 3.84 YY687 pKa = 10.65 EE688 pKa = 4.26 DD689 pKa = 5.47 APISKK694 pKa = 10.09 EE695 pKa = 3.94 EE696 pKa = 4.51 EE697 pKa = 3.73 IDD699 pKa = 3.65 EE700 pKa = 4.39 VEE702 pKa = 3.9 PSQFFEE708 pKa = 4.17 VLDD711 pKa = 3.73 MRR713 pKa = 11.84 KK714 pKa = 9.37 KK715 pKa = 10.31 LHH717 pKa = 6.71 SPTPDD722 pKa = 2.93 TKK724 pKa = 10.65 HH725 pKa = 6.98 AEE727 pKa = 4.08 TTEE730 pKa = 3.76 ILEE733 pKa = 4.17 PFMDD737 pKa = 3.63 KK738 pKa = 10.56 FKK740 pKa = 10.99 EE741 pKa = 3.92 LGYY744 pKa = 10.13 VEE746 pKa = 6.25 GMFEE750 pKa = 4.06 YY751 pKa = 9.84 PAAGHH756 pKa = 6.93 KK757 pKa = 9.42 MDD759 pKa = 4.61 TKK761 pKa = 10.85 SCVNAIIQHH770 pKa = 4.94 GTNVSEE776 pKa = 4.85 CPVPITPDD784 pKa = 3.24 EE785 pKa = 4.14 EE786 pKa = 4.54 DD787 pKa = 3.41 RR788 pKa = 11.84 VVDD791 pKa = 3.81 AVSAMVQEE799 pKa = 4.87 FSFVVDD805 pKa = 3.87 PNYY808 pKa = 10.83 KK809 pKa = 9.1 SLEE812 pKa = 4.02 YY813 pKa = 10.55 FEE815 pKa = 6.44 SIINTNQIEE824 pKa = 4.27 DD825 pKa = 3.72 RR826 pKa = 11.84 KK827 pKa = 10.24 SPGMPYY833 pKa = 10.21 LEE835 pKa = 4.51 EE836 pKa = 5.27 GMTTNAQVLDD846 pKa = 3.9 KK847 pKa = 11.51 LGVTWLYY854 pKa = 11.74 NKK856 pKa = 9.83 MIEE859 pKa = 4.29 CWNMSILYY867 pKa = 10.42 RR868 pKa = 11.84 MFLKK872 pKa = 10.98 YY873 pKa = 10.63 EE874 pKa = 3.8 PTKK877 pKa = 10.55 KK878 pKa = 10.43 KK879 pKa = 10.83 KK880 pKa = 9.31 IDD882 pKa = 2.84 NWMARR887 pKa = 11.84 VILSFPLHH895 pKa = 6.0 KK896 pKa = 9.46 TLQNIVLFKK905 pKa = 11.17 NLLSTDD911 pKa = 3.69 AVHH914 pKa = 6.96 KK915 pKa = 10.21 GSKK918 pKa = 9.22 FVWYY922 pKa = 8.18 TFNPKK927 pKa = 9.74 IPGHH931 pKa = 5.78 IASMARR937 pKa = 11.84 RR938 pKa = 11.84 FLNLVTFSSDD948 pKa = 2.86 KK949 pKa = 9.69 EE950 pKa = 4.4 TWDD953 pKa = 3.22 LSVRR957 pKa = 11.84 IEE959 pKa = 4.49 FYY961 pKa = 11.18 RR962 pKa = 11.84 MLARR966 pKa = 11.84 IVQNLCKK973 pKa = 9.8 KK974 pKa = 9.51 HH975 pKa = 6.6 PDD977 pKa = 3.47 MTVEE981 pKa = 4.33 AYY983 pKa = 9.68 KK984 pKa = 10.5 QVLLDD989 pKa = 3.49 IYY991 pKa = 9.79 DD992 pKa = 4.29 TIMQVGAEE1000 pKa = 4.08 GEE1002 pKa = 4.12 YY1003 pKa = 10.62 RR1004 pKa = 11.84 LNNGYY1009 pKa = 7.85 VLKK1012 pKa = 10.6 AVYY1015 pKa = 9.95 AGYY1018 pKa = 10.39 LKK1020 pKa = 10.74 SGWYY1024 pKa = 8.07 LTIIANSIMQVVVHH1038 pKa = 5.91 VLACFRR1044 pKa = 11.84 CGMSFEE1050 pKa = 4.96 EE1051 pKa = 5.5 IIDD1054 pKa = 3.87 LPFVAGGDD1062 pKa = 3.81 DD1063 pKa = 4.49 VIQHH1067 pKa = 6.49 LPDD1070 pKa = 5.19 HH1071 pKa = 6.43 IVDD1074 pKa = 4.53 RR1075 pKa = 11.84 YY1076 pKa = 10.28 IEE1078 pKa = 3.91 AHH1080 pKa = 6.0 ASFGIKK1086 pKa = 10.06 LKK1088 pKa = 10.81 LEE1090 pKa = 3.83 RR1091 pKa = 11.84 HH1092 pKa = 5.46 EE1093 pKa = 4.35 SFEE1096 pKa = 3.93 GSEE1099 pKa = 4.19 FFSNRR1104 pKa = 11.84 FFLDD1108 pKa = 4.08 SKK1110 pKa = 9.87 GKK1112 pKa = 9.32 WYY1114 pKa = 9.85 FVPQRR1119 pKa = 11.84 FCKK1122 pKa = 10.33 HH1123 pKa = 5.78 IEE1125 pKa = 4.22 SLLHH1129 pKa = 5.96 MKK1131 pKa = 10.36 PKK1133 pKa = 10.74 DD1134 pKa = 3.56 LFQGLSSKK1142 pKa = 11.02 LDD1144 pKa = 3.25 DD1145 pKa = 3.43 WCFKK1149 pKa = 11.34 DD1150 pKa = 4.14 EE1151 pKa = 4.09 VSQVFEE1157 pKa = 5.83 DD1158 pKa = 3.26 IMSVIRR1164 pKa = 11.84 TKK1166 pKa = 10.82 YY1167 pKa = 8.0 KK1168 pKa = 9.71 HH1169 pKa = 6.55 LYY1171 pKa = 8.77 VPSVDD1176 pKa = 3.0 KK1177 pKa = 10.68 TRR1179 pKa = 11.84 RR1180 pKa = 11.84 EE1181 pKa = 4.56 AINQARR1187 pKa = 11.84 GYY1189 pKa = 10.56 EE1190 pKa = 3.99 DD1191 pKa = 4.89 LPINAEE1197 pKa = 3.88 TAIACDD1203 pKa = 3.54 SQTQTDD1209 pKa = 3.99 EE1210 pKa = 4.2 KK1211 pKa = 11.31 VEE1213 pKa = 4.46 DD1214 pKa = 4.18 GGDD1217 pKa = 3.52 FRR1219 pKa = 11.84 AEE1221 pKa = 3.56 FEE1223 pKa = 4.52 AIKK1226 pKa = 10.84 KK1227 pKa = 8.69 EE1228 pKa = 4.06 MRR1230 pKa = 11.84 QMMHH1234 pKa = 6.47 TYY1236 pKa = 10.62 RR1237 pKa = 11.84 MQIDD1241 pKa = 3.93 ALTSTKK1247 pKa = 10.85 NKK1249 pKa = 9.14 LQKK1252 pKa = 10.28 KK1253 pKa = 8.38 VLHH1256 pKa = 6.77 LEE1258 pKa = 4.37 TQLVTAKK1265 pKa = 10.29 LKK1267 pKa = 11.19 NKK1269 pKa = 9.63 GVSNNIGKK1277 pKa = 9.9 RR1278 pKa = 11.84 KK1279 pKa = 9.77 KK1280 pKa = 10.18 DD1281 pKa = 3.64 VNSQSSDD1288 pKa = 3.22 PVLRR1292 pKa = 11.84 SPKK1295 pKa = 9.86 SPVGATVPTVDD1306 pKa = 4.98 QVVQPQSGGEE1316 pKa = 4.07 VFPPLPRR1323 pKa = 11.84 DD1324 pKa = 3.56 CC1325 pKa = 5.86
Molecular weight: 153.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.873
IPC2_protein 5.868
IPC_protein 5.944
Toseland 6.313
ProMoST 6.313
Dawson 6.237
Bjellqvist 6.237
Wikipedia 6.249
Rodwell 6.224
Grimsley 6.465
Solomon 6.237
Lehninger 6.224
Nozaki 6.517
DTASelect 6.693
Thurlkill 6.707
EMBOSS 6.678
Sillero 6.605
Patrickios 4.024
IPC_peptide 6.249
IPC2_peptide 6.561
IPC2.peptide.svr19 6.536
Protein with the highest isoelectric point:
>tr|A0A1L3KED1|A0A1L3KED1_9VIRU RNA-directed RNA polymerase OS=Changjiang sobemo-like virus 2 OX=1922801 PE=4 SV=1
MM1 pKa = 7.22 YY2 pKa = 10.38 KK3 pKa = 10.45 LHH5 pKa = 6.35 VKK7 pKa = 10.56 NNGLDD12 pKa = 3.49 TTIFNPNCSSEE23 pKa = 4.09 NLMSSARR30 pKa = 11.84 EE31 pKa = 3.62 QKK33 pKa = 10.09 QNKK36 pKa = 8.32 KK37 pKa = 9.65 NAYY40 pKa = 8.14 PDD42 pKa = 3.97 EE43 pKa = 4.53 YY44 pKa = 11.38 DD45 pKa = 3.82 NLPDD49 pKa = 3.79 SSFPYY54 pKa = 9.66 FDD56 pKa = 5.16 NYY58 pKa = 9.58 TGPYY62 pKa = 8.18 WSDD65 pKa = 2.84 GKK67 pKa = 10.79 FQSSVNNGQSKK78 pKa = 9.74 PLSKK82 pKa = 10.65 LDD84 pKa = 3.19 WHH86 pKa = 6.85 SRR88 pKa = 11.84 DD89 pKa = 3.65 HH90 pKa = 6.93 DD91 pKa = 3.7 ACFADD96 pKa = 4.49 GKK98 pKa = 10.69 DD99 pKa = 2.89 IAYY102 pKa = 10.52 CDD104 pKa = 3.36 DD105 pKa = 3.4 VYY107 pKa = 11.87 YY108 pKa = 11.43 NNTRR112 pKa = 11.84 DD113 pKa = 3.26 LDD115 pKa = 3.74 LRR117 pKa = 11.84 GRR119 pKa = 11.84 LIGIIPKK126 pKa = 9.47 LAHH129 pKa = 6.34 GGFSIFGQSGRR140 pKa = 11.84 VGGVADD146 pKa = 3.85 KK147 pKa = 7.77 MTRR150 pKa = 11.84 PTPAQSAGGHH160 pKa = 5.87 PSGPTSYY167 pKa = 10.82 SGVPTDD173 pKa = 3.33 SLKK176 pKa = 10.95 PIGGVYY182 pKa = 10.49 DD183 pKa = 4.5 GIGLVAKK190 pKa = 10.08 PSMPNSFPKK199 pKa = 10.64 HH200 pKa = 5.86 PDD202 pKa = 3.09 FGEE205 pKa = 4.2 STLLQTTYY213 pKa = 11.1 APEE216 pKa = 4.03 TQEE219 pKa = 3.84 PKK221 pKa = 10.85 VIDD224 pKa = 3.67 RR225 pKa = 11.84 QEE227 pKa = 3.97 LSEE230 pKa = 4.94 NPLNLNPTRR239 pKa = 11.84 DD240 pKa = 3.76 DD241 pKa = 3.62 QVGYY245 pKa = 8.65 TYY247 pKa = 10.65 EE248 pKa = 3.96 SKK250 pKa = 10.88 GRR252 pKa = 11.84 YY253 pKa = 8.65 NSQKK257 pKa = 8.27 TWQKK261 pKa = 9.7 PRR263 pKa = 11.84 KK264 pKa = 9.2 HH265 pKa = 5.95 KK266 pKa = 9.83 NQRR269 pKa = 11.84 KK270 pKa = 8.87 RR271 pKa = 11.84 RR272 pKa = 11.84 NKK274 pKa = 10.16 RR275 pKa = 11.84 KK276 pKa = 9.68 NKK278 pKa = 9.61 KK279 pKa = 9.49
Molecular weight: 31.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.685
IPC2_protein 8.653
IPC_protein 8.551
Toseland 9.165
ProMoST 9.092
Dawson 9.487
Bjellqvist 9.253
Wikipedia 9.677
Rodwell 9.706
Grimsley 9.575
Solomon 9.531
Lehninger 9.487
Nozaki 9.297
DTASelect 9.209
Thurlkill 9.326
EMBOSS 9.619
Sillero 9.443
Patrickios 4.609
IPC_peptide 9.531
IPC2_peptide 7.849
IPC2.peptide.svr19 7.724
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2001
279
1325
667.0
75.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.947 ± 1.177
1.549 ± 0.265
5.897 ± 0.646
5.297 ± 1.732
4.948 ± 0.75
5.847 ± 1.049
2.549 ± 0.516
5.097 ± 0.588
6.547 ± 0.766
7.296 ± 0.57
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.849 ± 0.586
4.798 ± 0.911
5.247 ± 0.983
3.198 ± 0.659
4.748 ± 0.626
7.996 ± 0.991
5.797 ± 0.889
7.796 ± 1.248
1.849 ± 0.274
4.748 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here