Changjiang sobemo-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KEG8|A0A1L3KEG8_9VIRU Capsid protein OS=Changjiang sobemo-like virus 2 OX=1922801 PE=3 SV=1
MM1 pKa = 7.6IVPKK5 pKa = 10.85NKK7 pKa = 9.32FCQSDD12 pKa = 3.71DD13 pKa = 3.41YY14 pKa = 11.57DD15 pKa = 3.58YY16 pKa = 11.52CSRR19 pKa = 11.84DD20 pKa = 3.46KK21 pKa = 11.17CWVCLIPLRR30 pKa = 11.84CSDD33 pKa = 5.26LIEE36 pKa = 5.01KK37 pKa = 9.66YY38 pKa = 10.14ISYY41 pKa = 10.61DD42 pKa = 3.08PYY44 pKa = 10.38EE45 pKa = 4.56AFGSEE50 pKa = 4.47YY51 pKa = 10.62EE52 pKa = 4.63SIVLPCEE59 pKa = 3.58RR60 pKa = 11.84LAALCYY66 pKa = 10.45YY67 pKa = 9.99EE68 pKa = 5.11SLWTEE73 pKa = 3.9VRR75 pKa = 11.84RR76 pKa = 11.84SSTEE80 pKa = 3.24WSIARR85 pKa = 11.84WYY87 pKa = 8.62STAVDD92 pKa = 3.21HH93 pKa = 7.26RR94 pKa = 11.84GRR96 pKa = 11.84PRR98 pKa = 11.84AFRR101 pKa = 11.84IVGTNLHH108 pKa = 6.77DD109 pKa = 4.27LAGVAAMPYY118 pKa = 8.65PPTMSEE124 pKa = 3.58MEE126 pKa = 4.62YY127 pKa = 10.2ILKK130 pKa = 9.81EE131 pKa = 4.21VPHH134 pKa = 7.29DD135 pKa = 4.98DD136 pKa = 3.7IPGEE140 pKa = 4.08VFKK143 pKa = 11.39YY144 pKa = 10.49LGGRR148 pKa = 11.84TKK150 pKa = 11.02LSFTLKK156 pKa = 10.05LFLMFGVLSGTIFSVEE172 pKa = 4.05ASDD175 pKa = 5.17QYY177 pKa = 11.61TSDD180 pKa = 3.43LVNTGEE186 pKa = 4.23LARR189 pKa = 11.84EE190 pKa = 3.78ITLWPVNEE198 pKa = 4.15SFRR201 pKa = 11.84MLEE204 pKa = 3.87HH205 pKa = 5.76TTISTTQCHH214 pKa = 5.8YY215 pKa = 11.48EE216 pKa = 3.85NGTCVEE222 pKa = 4.23YY223 pKa = 10.41PNLVNQVFDD232 pKa = 3.62WLRR235 pKa = 11.84VRR237 pKa = 11.84VRR239 pKa = 11.84NLCYY243 pKa = 10.63LLDD246 pKa = 4.23EE247 pKa = 4.54STLLARR253 pKa = 11.84HH254 pKa = 5.95SSSVAKK260 pKa = 10.26EE261 pKa = 3.37RR262 pKa = 11.84TRR264 pKa = 11.84FYY266 pKa = 11.47KK267 pKa = 10.71NIIVKK272 pKa = 10.22DD273 pKa = 3.79DD274 pKa = 3.66YY275 pKa = 11.47FRR277 pKa = 11.84AEE279 pKa = 4.09YY280 pKa = 10.89DD281 pKa = 4.21DD282 pKa = 5.07ISTAEE287 pKa = 3.89NEE289 pKa = 4.75LYY291 pKa = 11.03NFMMLFLSFLYY302 pKa = 10.42GINSGFWSLCILFINFLYY320 pKa = 10.41FMRR323 pKa = 11.84FAHH326 pKa = 6.02NFVVSTPLGFWIYY339 pKa = 10.54TKK341 pKa = 10.19VVWVLGIWLGYY352 pKa = 8.78YY353 pKa = 8.12VTYY356 pKa = 10.15HH357 pKa = 6.59ALVVLLAASISYY369 pKa = 9.23LHH371 pKa = 6.72SKK373 pKa = 9.63MIEE376 pKa = 3.94LAVVVVMAPYY386 pKa = 9.94HH387 pKa = 4.78IWSRR391 pKa = 11.84FRR393 pKa = 11.84RR394 pKa = 11.84TGVKK398 pKa = 10.04GFVRR402 pKa = 11.84EE403 pKa = 4.13MILPGSKK410 pKa = 8.86IEE412 pKa = 4.16KK413 pKa = 10.2FIGAKK418 pKa = 9.31QPAVAALVVEE428 pKa = 4.94VDD430 pKa = 4.22GKK432 pKa = 7.88WHH434 pKa = 5.8YY435 pKa = 11.43SAMCFRR441 pKa = 11.84YY442 pKa = 9.86EE443 pKa = 3.85NHH445 pKa = 6.48LVSAFHH451 pKa = 7.01PFAKK455 pKa = 10.12HH456 pKa = 4.43MSVGRR461 pKa = 11.84RR462 pKa = 11.84MGVAPNLLCKK472 pKa = 10.33KK473 pKa = 9.83RR474 pKa = 11.84WTHH477 pKa = 6.81DD478 pKa = 3.32FGAIQVIDD486 pKa = 3.97PSNYY490 pKa = 7.98IVDD493 pKa = 4.53FEE495 pKa = 4.34RR496 pKa = 11.84HH497 pKa = 5.58DD498 pKa = 3.7VFMLHH503 pKa = 6.27MSGEE507 pKa = 3.78FWDD510 pKa = 4.01RR511 pKa = 11.84VRR513 pKa = 11.84LPEE516 pKa = 4.18PKK518 pKa = 10.07KK519 pKa = 10.74SVSNSFNDD527 pKa = 3.32SATVFSYY534 pKa = 10.17MAGTLHH540 pKa = 6.6TSNGVLTSHH549 pKa = 6.78TNVDD553 pKa = 3.66YY554 pKa = 10.68PPNNFMAHH562 pKa = 5.43TCSTLPGFSGTPVFAANGAVRR583 pKa = 11.84GVHH586 pKa = 6.11IGSDD590 pKa = 3.33NYY592 pKa = 10.31EE593 pKa = 3.83EE594 pKa = 4.36RR595 pKa = 11.84NIMFRR600 pKa = 11.84IDD602 pKa = 3.45HH603 pKa = 6.32IKK605 pKa = 10.93YY606 pKa = 9.55YY607 pKa = 10.76LNKK610 pKa = 10.43NKK612 pKa = 8.07VTRR615 pKa = 11.84EE616 pKa = 3.98DD617 pKa = 3.35SSDD620 pKa = 3.56FQKK623 pKa = 11.08KK624 pKa = 7.11VLRR627 pKa = 11.84SMKK630 pKa = 9.64RR631 pKa = 11.84RR632 pKa = 11.84KK633 pKa = 9.86GKK635 pKa = 9.17EE636 pKa = 3.24AVYY639 pKa = 10.9YY640 pKa = 10.73FDD642 pKa = 6.44DD643 pKa = 4.25FDD645 pKa = 4.56EE646 pKa = 5.43DD647 pKa = 3.56NSVAYY652 pKa = 9.86DD653 pKa = 3.22IRR655 pKa = 11.84TGQIEE660 pKa = 4.27RR661 pKa = 11.84VPRR664 pKa = 11.84TEE666 pKa = 4.36EE667 pKa = 3.54EE668 pKa = 4.67DD669 pKa = 3.6EE670 pKa = 4.32EE671 pKa = 4.37FWRR674 pKa = 11.84VEE676 pKa = 3.71MQKK679 pKa = 10.5PVGLWGDD686 pKa = 3.84YY687 pKa = 10.65EE688 pKa = 4.26DD689 pKa = 5.47APISKK694 pKa = 10.09EE695 pKa = 3.94EE696 pKa = 4.51EE697 pKa = 3.73IDD699 pKa = 3.65EE700 pKa = 4.39VEE702 pKa = 3.9PSQFFEE708 pKa = 4.17VLDD711 pKa = 3.73MRR713 pKa = 11.84KK714 pKa = 9.37KK715 pKa = 10.31LHH717 pKa = 6.71SPTPDD722 pKa = 2.93TKK724 pKa = 10.65HH725 pKa = 6.98AEE727 pKa = 4.08TTEE730 pKa = 3.76ILEE733 pKa = 4.17PFMDD737 pKa = 3.63KK738 pKa = 10.56FKK740 pKa = 10.99EE741 pKa = 3.92LGYY744 pKa = 10.13VEE746 pKa = 6.25GMFEE750 pKa = 4.06YY751 pKa = 9.84PAAGHH756 pKa = 6.93KK757 pKa = 9.42MDD759 pKa = 4.61TKK761 pKa = 10.85SCVNAIIQHH770 pKa = 4.94GTNVSEE776 pKa = 4.85CPVPITPDD784 pKa = 3.24EE785 pKa = 4.14EE786 pKa = 4.54DD787 pKa = 3.41RR788 pKa = 11.84VVDD791 pKa = 3.81AVSAMVQEE799 pKa = 4.87FSFVVDD805 pKa = 3.87PNYY808 pKa = 10.83KK809 pKa = 9.1SLEE812 pKa = 4.02YY813 pKa = 10.55FEE815 pKa = 6.44SIINTNQIEE824 pKa = 4.27DD825 pKa = 3.72RR826 pKa = 11.84KK827 pKa = 10.24SPGMPYY833 pKa = 10.21LEE835 pKa = 4.51EE836 pKa = 5.27GMTTNAQVLDD846 pKa = 3.9KK847 pKa = 11.51LGVTWLYY854 pKa = 11.74NKK856 pKa = 9.83MIEE859 pKa = 4.29CWNMSILYY867 pKa = 10.42RR868 pKa = 11.84MFLKK872 pKa = 10.98YY873 pKa = 10.63EE874 pKa = 3.8PTKK877 pKa = 10.55KK878 pKa = 10.43KK879 pKa = 10.83KK880 pKa = 9.31IDD882 pKa = 2.84NWMARR887 pKa = 11.84VILSFPLHH895 pKa = 6.0KK896 pKa = 9.46TLQNIVLFKK905 pKa = 11.17NLLSTDD911 pKa = 3.69AVHH914 pKa = 6.96KK915 pKa = 10.21GSKK918 pKa = 9.22FVWYY922 pKa = 8.18TFNPKK927 pKa = 9.74IPGHH931 pKa = 5.78IASMARR937 pKa = 11.84RR938 pKa = 11.84FLNLVTFSSDD948 pKa = 2.86KK949 pKa = 9.69EE950 pKa = 4.4TWDD953 pKa = 3.22LSVRR957 pKa = 11.84IEE959 pKa = 4.49FYY961 pKa = 11.18RR962 pKa = 11.84MLARR966 pKa = 11.84IVQNLCKK973 pKa = 9.8KK974 pKa = 9.51HH975 pKa = 6.6PDD977 pKa = 3.47MTVEE981 pKa = 4.33AYY983 pKa = 9.68KK984 pKa = 10.5QVLLDD989 pKa = 3.49IYY991 pKa = 9.79DD992 pKa = 4.29TIMQVGAEE1000 pKa = 4.08GEE1002 pKa = 4.12YY1003 pKa = 10.62RR1004 pKa = 11.84LNNGYY1009 pKa = 7.85VLKK1012 pKa = 10.6AVYY1015 pKa = 9.95AGYY1018 pKa = 10.39LKK1020 pKa = 10.74SGWYY1024 pKa = 8.07LTIIANSIMQVVVHH1038 pKa = 5.91VLACFRR1044 pKa = 11.84CGMSFEE1050 pKa = 4.96EE1051 pKa = 5.5IIDD1054 pKa = 3.87LPFVAGGDD1062 pKa = 3.81DD1063 pKa = 4.49VIQHH1067 pKa = 6.49LPDD1070 pKa = 5.19HH1071 pKa = 6.43IVDD1074 pKa = 4.53RR1075 pKa = 11.84YY1076 pKa = 10.28IEE1078 pKa = 3.91AHH1080 pKa = 6.0ASFGIKK1086 pKa = 10.06LKK1088 pKa = 10.81LEE1090 pKa = 3.83RR1091 pKa = 11.84HH1092 pKa = 5.46EE1093 pKa = 4.35SFEE1096 pKa = 3.93GSEE1099 pKa = 4.19FFSNRR1104 pKa = 11.84FFLDD1108 pKa = 4.08SKK1110 pKa = 9.87GKK1112 pKa = 9.32WYY1114 pKa = 9.85FVPQRR1119 pKa = 11.84FCKK1122 pKa = 10.33HH1123 pKa = 5.78IEE1125 pKa = 4.22SLLHH1129 pKa = 5.96MKK1131 pKa = 10.36PKK1133 pKa = 10.74DD1134 pKa = 3.56LFQGLSSKK1142 pKa = 11.02LDD1144 pKa = 3.25DD1145 pKa = 3.43WCFKK1149 pKa = 11.34DD1150 pKa = 4.14EE1151 pKa = 4.09VSQVFEE1157 pKa = 5.83DD1158 pKa = 3.26IMSVIRR1164 pKa = 11.84TKK1166 pKa = 10.82YY1167 pKa = 8.0KK1168 pKa = 9.71HH1169 pKa = 6.55LYY1171 pKa = 8.77VPSVDD1176 pKa = 3.0KK1177 pKa = 10.68TRR1179 pKa = 11.84RR1180 pKa = 11.84EE1181 pKa = 4.56AINQARR1187 pKa = 11.84GYY1189 pKa = 10.56EE1190 pKa = 3.99DD1191 pKa = 4.89LPINAEE1197 pKa = 3.88TAIACDD1203 pKa = 3.54SQTQTDD1209 pKa = 3.99EE1210 pKa = 4.2KK1211 pKa = 11.31VEE1213 pKa = 4.46DD1214 pKa = 4.18GGDD1217 pKa = 3.52FRR1219 pKa = 11.84AEE1221 pKa = 3.56FEE1223 pKa = 4.52AIKK1226 pKa = 10.84KK1227 pKa = 8.69EE1228 pKa = 4.06MRR1230 pKa = 11.84QMMHH1234 pKa = 6.47TYY1236 pKa = 10.62RR1237 pKa = 11.84MQIDD1241 pKa = 3.93ALTSTKK1247 pKa = 10.85NKK1249 pKa = 9.14LQKK1252 pKa = 10.28KK1253 pKa = 8.38VLHH1256 pKa = 6.77LEE1258 pKa = 4.37TQLVTAKK1265 pKa = 10.29LKK1267 pKa = 11.19NKK1269 pKa = 9.63GVSNNIGKK1277 pKa = 9.9RR1278 pKa = 11.84KK1279 pKa = 9.77KK1280 pKa = 10.18DD1281 pKa = 3.64VNSQSSDD1288 pKa = 3.22PVLRR1292 pKa = 11.84SPKK1295 pKa = 9.86SPVGATVPTVDD1306 pKa = 4.98QVVQPQSGGEE1316 pKa = 4.07VFPPLPRR1323 pKa = 11.84DD1324 pKa = 3.56CC1325 pKa = 5.86

Molecular weight:
153.1 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KED1|A0A1L3KED1_9VIRU RNA-directed RNA polymerase OS=Changjiang sobemo-like virus 2 OX=1922801 PE=4 SV=1
MM1 pKa = 7.22YY2 pKa = 10.38KK3 pKa = 10.45LHH5 pKa = 6.35VKK7 pKa = 10.56NNGLDD12 pKa = 3.49TTIFNPNCSSEE23 pKa = 4.09NLMSSARR30 pKa = 11.84EE31 pKa = 3.62QKK33 pKa = 10.09QNKK36 pKa = 8.32KK37 pKa = 9.65NAYY40 pKa = 8.14PDD42 pKa = 3.97EE43 pKa = 4.53YY44 pKa = 11.38DD45 pKa = 3.82NLPDD49 pKa = 3.79SSFPYY54 pKa = 9.66FDD56 pKa = 5.16NYY58 pKa = 9.58TGPYY62 pKa = 8.18WSDD65 pKa = 2.84GKK67 pKa = 10.79FQSSVNNGQSKK78 pKa = 9.74PLSKK82 pKa = 10.65LDD84 pKa = 3.19WHH86 pKa = 6.85SRR88 pKa = 11.84DD89 pKa = 3.65HH90 pKa = 6.93DD91 pKa = 3.7ACFADD96 pKa = 4.49GKK98 pKa = 10.69DD99 pKa = 2.89IAYY102 pKa = 10.52CDD104 pKa = 3.36DD105 pKa = 3.4VYY107 pKa = 11.87YY108 pKa = 11.43NNTRR112 pKa = 11.84DD113 pKa = 3.26LDD115 pKa = 3.74LRR117 pKa = 11.84GRR119 pKa = 11.84LIGIIPKK126 pKa = 9.47LAHH129 pKa = 6.34GGFSIFGQSGRR140 pKa = 11.84VGGVADD146 pKa = 3.85KK147 pKa = 7.77MTRR150 pKa = 11.84PTPAQSAGGHH160 pKa = 5.87PSGPTSYY167 pKa = 10.82SGVPTDD173 pKa = 3.33SLKK176 pKa = 10.95PIGGVYY182 pKa = 10.49DD183 pKa = 4.5GIGLVAKK190 pKa = 10.08PSMPNSFPKK199 pKa = 10.64HH200 pKa = 5.86PDD202 pKa = 3.09FGEE205 pKa = 4.2STLLQTTYY213 pKa = 11.1APEE216 pKa = 4.03TQEE219 pKa = 3.84PKK221 pKa = 10.85VIDD224 pKa = 3.67RR225 pKa = 11.84QEE227 pKa = 3.97LSEE230 pKa = 4.94NPLNLNPTRR239 pKa = 11.84DD240 pKa = 3.76DD241 pKa = 3.62QVGYY245 pKa = 8.65TYY247 pKa = 10.65EE248 pKa = 3.96SKK250 pKa = 10.88GRR252 pKa = 11.84YY253 pKa = 8.65NSQKK257 pKa = 8.27TWQKK261 pKa = 9.7PRR263 pKa = 11.84KK264 pKa = 9.2HH265 pKa = 5.95KK266 pKa = 9.83NQRR269 pKa = 11.84KK270 pKa = 8.87RR271 pKa = 11.84RR272 pKa = 11.84NKK274 pKa = 10.16RR275 pKa = 11.84KK276 pKa = 9.68NKK278 pKa = 9.61KK279 pKa = 9.49

Molecular weight:
31.36 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2001

279

1325

667.0

75.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.947 ± 1.177

1.549 ± 0.265

5.897 ± 0.646

5.297 ± 1.732

4.948 ± 0.75

5.847 ± 1.049

2.549 ± 0.516

5.097 ± 0.588

6.547 ± 0.766

7.296 ± 0.57

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.849 ± 0.586

4.798 ± 0.911

5.247 ± 0.983

3.198 ± 0.659

4.748 ± 0.626

7.996 ± 0.991

5.797 ± 0.889

7.796 ± 1.248

1.849 ± 0.274

4.748 ± 0.209

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski