Paraperlucidibaca baekdonensis
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2146 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3E0H8H3|A0A3E0H8H3_9GAMM LytTR family two component transcriptional regulator OS=Paraperlucidibaca baekdonensis OX=748120 GN=DFR26_0237 PE=4 SV=1
MM1 pKa = 7.1 SQLPAEE7 pKa = 4.61 LKK9 pKa = 10.34 YY10 pKa = 10.61 AASHH14 pKa = 5.17 EE15 pKa = 4.21 WARR18 pKa = 11.84 LDD20 pKa = 3.64 GDD22 pKa = 4.09 VVTVGITDD30 pKa = 4.04 HH31 pKa = 6.8 AQDD34 pKa = 3.61 ALGDD38 pKa = 3.85 LVYY41 pKa = 11.09 VEE43 pKa = 5.09 LPNVGDD49 pKa = 3.85 AVSAGQEE56 pKa = 3.86 AGVVEE61 pKa = 4.56 SVKK64 pKa = 10.59 AASDD68 pKa = 3.41 IYY70 pKa = 11.21 APVSGEE76 pKa = 3.63 IIAINDD82 pKa = 3.93 ALVDD86 pKa = 3.53 APEE89 pKa = 4.18 IVNTEE94 pKa = 4.35 PYY96 pKa = 10.5 AGGWLYY102 pKa = 10.44 QIKK105 pKa = 10.37 ISDD108 pKa = 3.67 AAEE111 pKa = 4.17 LGEE114 pKa = 4.19 LLSAEE119 pKa = 4.32 EE120 pKa = 3.95 YY121 pKa = 10.38 AAKK124 pKa = 9.78 QDD126 pKa = 3.69 HH127 pKa = 6.32
Molecular weight: 13.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.91
IPC_protein 3.859
Toseland 3.668
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.694
Grimsley 3.579
Solomon 3.821
Lehninger 3.783
Nozaki 3.961
DTASelect 4.139
Thurlkill 3.719
EMBOSS 3.757
Sillero 3.973
Patrickios 1.888
IPC_peptide 3.821
IPC2_peptide 3.961
IPC2.peptide.svr19 3.875
Protein with the highest isoelectric point:
>tr|A0A3E0HA92|A0A3E0HA92_9GAMM Outer membrane protein assembly factor BamD OS=Paraperlucidibaca baekdonensis OX=748120 GN=bamD PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSTIKK11 pKa = 10.33 QKK13 pKa = 9.06 RR14 pKa = 11.84 THH16 pKa = 6.13 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.33 NGRR28 pKa = 11.84 AVIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.69 GRR39 pKa = 11.84 KK40 pKa = 8.88 RR41 pKa = 11.84 LTVV44 pKa = 3.11
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2146
0
2146
709905
30
2355
330.8
36.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.891 ± 0.075
0.977 ± 0.016
5.499 ± 0.036
5.301 ± 0.05
3.548 ± 0.034
7.502 ± 0.049
2.411 ± 0.029
5.147 ± 0.04
3.422 ± 0.042
11.043 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.624 ± 0.029
3.043 ± 0.036
4.71 ± 0.036
4.265 ± 0.036
6.371 ± 0.048
6.039 ± 0.042
5.111 ± 0.038
7.227 ± 0.043
1.436 ± 0.022
2.432 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here