Streptococcus phage Javan443

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ACN7|A0A4D6ACN7_9CAUD Capsid protein OS=Streptococcus phage Javan443 OX=2548169 GN=Javan443_0050 PE=4 SV=1
MM1 pKa = 7.35SKK3 pKa = 10.32GYY5 pKa = 10.32KK6 pKa = 10.24VIDD9 pKa = 3.32IGSNPEE15 pKa = 3.87DD16 pKa = 3.69DD17 pKa = 4.01VTFGTCEE24 pKa = 3.65LCMSYY29 pKa = 11.11GNEE32 pKa = 3.57VDD34 pKa = 4.11NPYY37 pKa = 11.02LVIEE41 pKa = 4.81KK42 pKa = 10.11PDD44 pKa = 3.53GTTEE48 pKa = 4.07EE49 pKa = 3.62VDD51 pKa = 2.77IYY53 pKa = 9.32YY54 pKa = 9.88WSWGDD59 pKa = 3.39YY60 pKa = 9.99FEE62 pKa = 5.57YY63 pKa = 11.09YY64 pKa = 9.51IDD66 pKa = 3.72NVVEE70 pKa = 5.43FSAFLSEE77 pKa = 4.88QDD79 pKa = 3.19IDD81 pKa = 4.0DD82 pKa = 4.8KK83 pKa = 11.49EE84 pKa = 4.33FEE86 pKa = 4.43EE87 pKa = 4.99NSTSVIINLINEE99 pKa = 4.05YY100 pKa = 10.84DD101 pKa = 3.83CLKK104 pKa = 10.92LEE106 pKa = 4.72EE107 pKa = 4.45EE108 pKa = 4.69DD109 pKa = 3.65

Molecular weight: 12.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ACB6|A0A4D6ACB6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan443 OX=2548169 GN=Javan443_0016 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.15NGPKK7 pKa = 10.23GGGRR11 pKa = 11.84IGAVKK16 pKa = 10.31GRR18 pKa = 11.84SQSHH22 pKa = 5.0NPKK25 pKa = 9.14TGLYY29 pKa = 7.7TKK31 pKa = 10.29RR32 pKa = 11.84DD33 pKa = 3.38TSTGKK38 pKa = 10.62FMDD41 pKa = 3.68TKK43 pKa = 8.85TTGGKK48 pKa = 9.61FKK50 pKa = 10.73GVRR53 pKa = 11.84TEE55 pKa = 3.81KK56 pKa = 10.97

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

11795

42

1380

178.7

20.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.766 ± 0.805

0.534 ± 0.11

6.189 ± 0.347

7.266 ± 0.415

4.256 ± 0.254

6.316 ± 0.376

1.204 ± 0.143

7.011 ± 0.307

8.792 ± 0.287

8.529 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.526 ± 0.215

6.198 ± 0.258

2.408 ± 0.157

3.798 ± 0.223

3.595 ± 0.227

6.783 ± 0.433

6.961 ± 0.423

6.189 ± 0.223

1.043 ± 0.114

3.637 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski