Capybara microvirus Cap1_SP_59
Average proteome isoelectric point is 5.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W3W8|A0A4P8W3W8_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_59 OX=2584784 PE=4 SV=1
MM1 pKa = 6.83 NTNLRR6 pKa = 11.84 KK7 pKa = 9.36 IRR9 pKa = 11.84 CNFNPIVEE17 pKa = 4.11 VRR19 pKa = 11.84 PVKK22 pKa = 10.32 IGEE25 pKa = 4.81 DD26 pKa = 2.88 IDD28 pKa = 3.6 IRR30 pKa = 11.84 FVDD33 pKa = 5.34 PSASDD38 pKa = 3.85 LPSPDD43 pKa = 2.94 SMRR46 pKa = 11.84 LEE48 pKa = 4.31 RR49 pKa = 11.84 LLSAGYY55 pKa = 10.03 RR56 pKa = 11.84 PEE58 pKa = 4.09 QVSPAFMTPTAAHH71 pKa = 6.04 TLEE74 pKa = 5.17 DD75 pKa = 3.77 MAEE78 pKa = 4.03 SAINSIVEE86 pKa = 3.87 TSNNQEE92 pKa = 3.91 SS93 pKa = 3.55
Molecular weight: 10.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.81
IPC2_protein 4.774
IPC_protein 4.609
Toseland 4.457
ProMoST 4.711
Dawson 4.546
Bjellqvist 4.698
Wikipedia 4.418
Rodwell 4.444
Grimsley 4.368
Solomon 4.546
Lehninger 4.495
Nozaki 4.66
DTASelect 4.8
Thurlkill 4.469
EMBOSS 4.431
Sillero 4.711
Patrickios 4.253
IPC_peptide 4.546
IPC2_peptide 4.711
IPC2.peptide.svr19 4.677
Protein with the highest isoelectric point:
>tr|A0A4P8W3S1|A0A4P8W3S1_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_59 OX=2584784 PE=4 SV=1
MM1 pKa = 7.85 CISKK5 pKa = 10.28 IGIRR9 pKa = 11.84 SKK11 pKa = 11.19 DD12 pKa = 3.46 LVYY15 pKa = 11.03 NQYY18 pKa = 8.95 TPAFRR23 pKa = 11.84 SVPCGICPQCLNTKK37 pKa = 9.54 RR38 pKa = 11.84 NNMYY42 pKa = 9.92 IRR44 pKa = 11.84 SFFEE48 pKa = 3.74 WQHH51 pKa = 4.42 TLKK54 pKa = 10.92 EE55 pKa = 4.28 GGFVLFPTYY64 pKa = 10.08 TFQDD68 pKa = 2.76 SDD70 pKa = 3.87 LPYY73 pKa = 10.43 FDD75 pKa = 4.77 MRR77 pKa = 11.84 DD78 pKa = 3.49 GSLHH82 pKa = 6.88 RR83 pKa = 11.84 GVQDD87 pKa = 3.46 SYY89 pKa = 11.94 HH90 pKa = 7.36 LDD92 pKa = 4.38 DD93 pKa = 5.34 YY94 pKa = 11.4 ICPCFDD100 pKa = 3.54 FSDD103 pKa = 3.29 IRR105 pKa = 11.84 GTKK108 pKa = 10.26 GIVKK112 pKa = 10.25 AIRR115 pKa = 11.84 SHH117 pKa = 7.14 ARR119 pKa = 11.84 DD120 pKa = 3.1 VWNVVGVKK128 pKa = 9.88 YY129 pKa = 8.74 VVCPEE134 pKa = 4.03 YY135 pKa = 11.12 GPTPNKK141 pKa = 6.35 THH143 pKa = 7.06 RR144 pKa = 11.84 PHH146 pKa = 6.15 FHH148 pKa = 7.15 CLFFCPPSPLGSLDD162 pKa = 3.68 GDD164 pKa = 3.53 LQRR167 pKa = 11.84 RR168 pKa = 11.84 YY169 pKa = 10.0 KK170 pKa = 11.14 DD171 pKa = 4.06 DD172 pKa = 3.41 MAKK175 pKa = 10.38 FCLKK179 pKa = 9.95 VWSEE183 pKa = 4.24 RR184 pKa = 11.84 KK185 pKa = 9.18 GHH187 pKa = 5.44 GTASYY192 pKa = 9.77 SKK194 pKa = 10.33 KK195 pKa = 9.58 YY196 pKa = 10.87 GPFIEE201 pKa = 4.33 SFKK204 pKa = 9.83 GIKK207 pKa = 9.31 YY208 pKa = 9.41 ASKK211 pKa = 11.05 YY212 pKa = 4.71 MTKK215 pKa = 10.57 DD216 pKa = 3.42 VVDD219 pKa = 3.96 LCEE222 pKa = 3.95 NCPRR226 pKa = 11.84 LKK228 pKa = 10.63 KK229 pKa = 10.12 FLHH232 pKa = 6.52 HH233 pKa = 5.91 NTYY236 pKa = 10.72 DD237 pKa = 3.56 NKK239 pKa = 10.28 VFLPDD244 pKa = 2.96 NWQEE248 pKa = 3.7 IPLYY252 pKa = 9.18 EE253 pKa = 4.62 LPYY256 pKa = 9.21 QYY258 pKa = 10.24 RR259 pKa = 11.84 YY260 pKa = 10.24 KK261 pKa = 10.55 AIKK264 pKa = 9.74 PFLPRR269 pKa = 11.84 PLWCKK274 pKa = 10.55 GFGEE278 pKa = 5.46 SILQLLTDD286 pKa = 3.88 STDD289 pKa = 3.41 EE290 pKa = 3.96 QLTDD294 pKa = 4.13 LLVNGIKK301 pKa = 9.47 IDD303 pKa = 3.84 GDD305 pKa = 3.19 ISLDD309 pKa = 3.54 GKK311 pKa = 10.75 QNTYY315 pKa = 10.49 QIPRR319 pKa = 11.84 YY320 pKa = 8.8 IQQKK324 pKa = 6.86 FTWHH328 pKa = 6.23 TFDD331 pKa = 5.61 GYY333 pKa = 11.06 DD334 pKa = 3.1 IYY336 pKa = 11.52 NSRR339 pKa = 11.84 LSRR342 pKa = 11.84 ALRR345 pKa = 11.84 NVYY348 pKa = 7.16 EE349 pKa = 3.78 QRR351 pKa = 11.84 LNQKK355 pKa = 10.07 SLLLRR360 pKa = 11.84 NFFYY364 pKa = 10.96 NSHH367 pKa = 6.9 KK368 pKa = 10.8 YY369 pKa = 7.1 MHH371 pKa = 6.99 IWNDD375 pKa = 3.07 LHH377 pKa = 9.12 SPFDD381 pKa = 4.19 LKK383 pKa = 11.3 DD384 pKa = 3.47 LLSRR388 pKa = 11.84 ISPTQLACYY397 pKa = 9.87 SEE399 pKa = 4.26 IMCTNFAKK407 pKa = 10.55 SDD409 pKa = 3.78 FEE411 pKa = 5.88 DD412 pKa = 3.83 INSLPFPDD420 pKa = 4.01 VEE422 pKa = 4.36 YY423 pKa = 11.37 KK424 pKa = 10.91 EE425 pKa = 4.19 MTLKK429 pKa = 11.02 YY430 pKa = 8.58 MLDD433 pKa = 3.53 RR434 pKa = 11.84 IIYY437 pKa = 7.25 HH438 pKa = 6.4 KK439 pKa = 10.64 PNIKK443 pKa = 10.07 CLPHH447 pKa = 6.62 LKK449 pKa = 10.7 GLFKK453 pKa = 10.44 PLEE456 pKa = 3.78 AHH458 pKa = 6.63 KK459 pKa = 10.29 PYY461 pKa = 10.73 INDD464 pKa = 3.36 EE465 pKa = 4.2 FSLLLHH471 pKa = 6.82 FYY473 pKa = 9.39 SKK475 pKa = 11.02 CMQEE479 pKa = 4.67 DD480 pKa = 4.02 VNKK483 pKa = 10.13 QLYY486 pKa = 9.37 LRR488 pKa = 11.84 RR489 pKa = 11.84 KK490 pKa = 8.74 KK491 pKa = 9.95 RR492 pKa = 11.84 HH493 pKa = 6.07 HH494 pKa = 6.68 DD495 pKa = 4.16 AIVQAEE501 pKa = 4.46 LLPTLIKK508 pKa = 10.76 YY509 pKa = 9.28 EE510 pKa = 4.18 YY511 pKa = 10.82
Molecular weight: 60.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.841
IPC2_protein 8.009
IPC_protein 7.79
Toseland 7.512
ProMoST 8.434
Dawson 8.551
Bjellqvist 8.843
Wikipedia 8.507
Rodwell 8.595
Grimsley 7.556
Solomon 8.609
Lehninger 8.609
Nozaki 8.975
DTASelect 8.595
Thurlkill 8.653
EMBOSS 8.726
Sillero 8.946
Patrickios 3.884
IPC_peptide 8.595
IPC2_peptide 7.746
IPC2.peptide.svr19 7.976
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1687
93
695
421.8
48.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.343 ± 2.583
1.6 ± 0.624
6.461 ± 0.836
5.216 ± 1.096
5.039 ± 1.04
4.861 ± 0.233
2.134 ± 0.576
5.098 ± 0.369
6.52 ± 0.671
9.01 ± 0.634
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.253 ± 0.369
6.165 ± 0.873
4.149 ± 0.866
5.157 ± 1.406
4.505 ± 0.413
8.536 ± 1.107
5.75 ± 0.983
4.683 ± 0.88
0.948 ± 0.147
5.572 ± 0.818
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here