Capybara microvirus Cap3_SP_645
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVV3|A0A4V1FVV3_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_645 OX=2585486 PE=4 SV=1
MM1 pKa = 7.3 KK2 pKa = 9.93 MKK4 pKa = 10.53 VQVPFLRR11 pKa = 11.84 SAFNYY16 pKa = 10.44 DD17 pKa = 3.22 RR18 pKa = 11.84 DD19 pKa = 3.97 LVSDD23 pKa = 3.79 EE24 pKa = 4.73 TGLKK28 pKa = 10.84 CMDD31 pKa = 5.15 DD32 pKa = 4.73 SLTQQQFKK40 pKa = 11.04 EE41 pKa = 4.03 EE42 pKa = 4.0 ADD44 pKa = 3.2 INTIVDD50 pKa = 3.59 RR51 pKa = 11.84 FMKK54 pKa = 10.37 SGVLPTPAVMPQYY67 pKa = 10.78 IDD69 pKa = 3.43 FEE71 pKa = 4.99 GVFDD75 pKa = 4.26 YY76 pKa = 9.51 QTAMNVVRR84 pKa = 11.84 QADD87 pKa = 4.01 EE88 pKa = 3.67 QFMRR92 pKa = 11.84 MDD94 pKa = 3.45 AKK96 pKa = 10.48 IRR98 pKa = 11.84 ARR100 pKa = 11.84 FNNSPQEE107 pKa = 4.02 FLSFFADD114 pKa = 3.58 PANTEE119 pKa = 3.65 EE120 pKa = 5.07 AIRR123 pKa = 11.84 LGLAVPQAVAEE134 pKa = 4.38 TSVSAAEE141 pKa = 4.1 PTSKK145 pKa = 10.72 SEE147 pKa = 4.0 SS148 pKa = 3.51
Molecular weight: 16.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.654
IPC2_protein 4.533
IPC_protein 4.431
Toseland 4.253
ProMoST 4.546
Dawson 4.38
Bjellqvist 4.533
Wikipedia 4.279
Rodwell 4.266
Grimsley 4.164
Solomon 4.38
Lehninger 4.329
Nozaki 4.495
DTASelect 4.673
Thurlkill 4.266
EMBOSS 4.291
Sillero 4.533
Patrickios 4.202
IPC_peptide 4.38
IPC2_peptide 4.52
IPC2.peptide.svr19 4.491
Protein with the highest isoelectric point:
>tr|A0A4P8W6A3|A0A4P8W6A3_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_645 OX=2585486 PE=4 SV=1
MM1 pKa = 7.72 RR2 pKa = 11.84 KK3 pKa = 9.74 AGLKK7 pKa = 9.48 FRR9 pKa = 11.84 YY10 pKa = 8.82 VASGEE15 pKa = 4.13 YY16 pKa = 10.63 GEE18 pKa = 4.45 QTRR21 pKa = 11.84 RR22 pKa = 11.84 PHH24 pKa = 5.38 FHH26 pKa = 6.44 IALFGMDD33 pKa = 4.1 FNSDD37 pKa = 2.82 RR38 pKa = 11.84 VLFGRR43 pKa = 11.84 SSNGDD48 pKa = 2.95 RR49 pKa = 11.84 TYY51 pKa = 11.19 ISATVAKK58 pKa = 9.77 HH59 pKa = 4.71 WADD62 pKa = 3.6 SNGVPIGNHH71 pKa = 6.97 LIGEE75 pKa = 4.61 LNFEE79 pKa = 4.08 SAAYY83 pKa = 8.17 IARR86 pKa = 11.84 YY87 pKa = 8.61 ILKK90 pKa = 10.04 KK91 pKa = 10.41 IKK93 pKa = 10.65 ASDD96 pKa = 3.38 KK97 pKa = 11.1 LMPLPLYY104 pKa = 10.36 SDD106 pKa = 3.91 KK107 pKa = 10.69 EE108 pKa = 4.33 TGEE111 pKa = 4.86 LIFPNPEE118 pKa = 3.42 FMLMSKK124 pKa = 10.43 GISKK128 pKa = 9.99 GWFRR132 pKa = 11.84 DD133 pKa = 3.5 YY134 pKa = 11.3 FMSDD138 pKa = 3.17 VFPHH142 pKa = 6.58 GSVITQQGSRR152 pKa = 11.84 APVPRR157 pKa = 11.84 YY158 pKa = 9.59 YY159 pKa = 11.08 KK160 pKa = 10.71 NLLKK164 pKa = 10.72 EE165 pKa = 4.05 IGHH168 pKa = 6.88 DD169 pKa = 3.98 LALDD173 pKa = 3.46 MQFRR177 pKa = 11.84 QSVRR181 pKa = 11.84 AEE183 pKa = 3.74 ADD185 pKa = 3.15 AEE187 pKa = 4.24 MKK189 pKa = 10.32 KK190 pKa = 10.54 YY191 pKa = 10.54 EE192 pKa = 4.05 NQPVRR197 pKa = 11.84 KK198 pKa = 8.38 IARR201 pKa = 11.84 RR202 pKa = 11.84 TVSEE206 pKa = 4.07 SRR208 pKa = 11.84 VNQSKK213 pKa = 10.99 RR214 pKa = 11.84 ILL216 pKa = 3.66
Molecular weight: 24.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.117
IPC2_protein 9.268
IPC_protein 9.282
Toseland 9.882
ProMoST 9.604
Dawson 10.116
Bjellqvist 9.794
Wikipedia 10.292
Rodwell 10.467
Grimsley 10.204
Solomon 10.145
Lehninger 10.101
Nozaki 9.853
DTASelect 9.794
Thurlkill 9.955
EMBOSS 10.292
Sillero 10.014
Patrickios 9.663
IPC_peptide 10.145
IPC2_peptide 8.287
IPC2.peptide.svr19 8.286
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1265
89
536
253.0
28.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.012 ± 0.53
0.237 ± 0.121
5.771 ± 0.881
4.348 ± 1.05
5.375 ± 0.952
7.273 ± 1.225
1.581 ± 0.426
4.19 ± 0.445
4.269 ± 0.915
7.431 ± 0.399
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.688 ± 0.619
5.534 ± 0.436
4.664 ± 0.718
5.771 ± 0.843
5.771 ± 0.845
7.905 ± 0.414
6.561 ± 1.143
6.877 ± 0.976
0.87 ± 0.241
3.874 ± 0.587
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here