Gordonia phage Cleo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Emalynvirus; unclassified Emalynvirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VWF9|A0A649VWF9_9CAUD Deoxycytidylate deaminase OS=Gordonia phage Cleo OX=2656531 GN=39 PE=4 SV=1
MM1 pKa = 7.35TFFAITALDD10 pKa = 3.99DD11 pKa = 4.29PEE13 pKa = 6.88DD14 pKa = 4.03YY15 pKa = 11.43ADD17 pKa = 5.42GIAPTALAFLEE28 pKa = 4.69TIGVKK33 pKa = 10.02IGPRR37 pKa = 11.84PGDD40 pKa = 3.59GLCGVDD46 pKa = 3.76VEE48 pKa = 5.49SPAALQMAIQMANQVVDD65 pKa = 3.84SADD68 pKa = 3.56TDD70 pKa = 3.55EE71 pKa = 4.5DD72 pKa = 4.0TCYY75 pKa = 10.58HH76 pKa = 6.34WLGLSGDD83 pKa = 4.37DD84 pKa = 4.14DD85 pKa = 5.16LDD87 pKa = 4.16YY88 pKa = 11.36GGSYY92 pKa = 9.99ILEE95 pKa = 4.28ALEE98 pKa = 4.37TTCEE102 pKa = 3.97KK103 pKa = 10.78LGVVFDD109 pKa = 4.69PEE111 pKa = 4.1YY112 pKa = 11.24DD113 pKa = 3.77STDD116 pKa = 2.8LHH118 pKa = 6.35AKK120 pKa = 8.08VRR122 pKa = 11.84AWGMSQGFHH131 pKa = 5.84MLL133 pKa = 3.41

Molecular weight: 14.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VY47|A0A649VY47_9CAUD Uncharacterized protein OS=Gordonia phage Cleo OX=2656531 GN=62 PE=4 SV=1
MM1 pKa = 7.29LHH3 pKa = 6.48FDD5 pKa = 2.91IRR7 pKa = 11.84LRR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 9.2HH12 pKa = 6.65IRR14 pKa = 11.84VTAYY18 pKa = 9.01SRR20 pKa = 11.84VRR22 pKa = 11.84PGHH25 pKa = 5.31RR26 pKa = 11.84AVRR29 pKa = 11.84RR30 pKa = 11.84EE31 pKa = 3.92WNRR34 pKa = 11.84VGPVRR39 pKa = 11.84LLTKK43 pKa = 9.57CQWAFYY49 pKa = 10.03AHH51 pKa = 6.19FRR53 pKa = 11.84KK54 pKa = 8.66GTTMASAADD63 pKa = 4.06YY64 pKa = 10.67IRR66 pKa = 11.84LAFRR70 pKa = 11.84STTQEE75 pKa = 3.75WISAGEE81 pKa = 4.07LYY83 pKa = 11.02DD84 pKa = 5.23FVVTQNGVYY93 pKa = 10.26PSTYY97 pKa = 10.16KK98 pKa = 10.53NARR101 pKa = 11.84KK102 pKa = 9.81AIGLQSEE109 pKa = 4.42RR110 pKa = 11.84RR111 pKa = 11.84GKK113 pKa = 9.57RR114 pKa = 11.84WFYY117 pKa = 10.82RR118 pKa = 11.84LPEE121 pKa = 4.36GQLGRR126 pKa = 11.84DD127 pKa = 4.24LITLRR132 pKa = 11.84KK133 pKa = 9.11LQAEE137 pKa = 4.37RR138 pKa = 11.84PWDD141 pKa = 3.76VFLMDD146 pKa = 4.08MKK148 pKa = 10.71IALKK152 pKa = 9.67EE153 pKa = 4.09LKK155 pKa = 10.01NQQPTT160 pKa = 3.24

Molecular weight: 19.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

14537

60

1704

230.7

25.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.72 ± 0.557

0.715 ± 0.133

6.817 ± 0.304

5.716 ± 0.338

3.24 ± 0.201

8.619 ± 0.461

1.823 ± 0.171

4.747 ± 0.228

5.042 ± 0.284

8.097 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.676 ± 0.153

3.35 ± 0.168

5.441 ± 0.246

3.708 ± 0.199

6.349 ± 0.428

5.71 ± 0.272

6.122 ± 0.271

7.051 ± 0.23

2.27 ± 0.148

2.786 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski