Amino acid dipepetide frequency for Pseudomonas phage JBD30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.208AlaAla: 19.208 ± 3.209
1.36AlaCys: 1.36 ± 0.396
8.244AlaAsp: 8.244 ± 0.732
8.924AlaGlu: 8.924 ± 1.458
2.635AlaPhe: 2.635 ± 0.476
9.349AlaGly: 9.349 ± 1.334
1.7AlaHis: 1.7 ± 0.307
6.459AlaIle: 6.459 ± 0.658
4.08AlaLys: 4.08 ± 0.657
13.938AlaLeu: 13.938 ± 1.243
3.4AlaMet: 3.4 ± 0.441
3.315AlaAsn: 3.315 ± 0.611
5.609AlaPro: 5.609 ± 0.668
5.864AlaGln: 5.864 ± 0.864
10.199AlaArg: 10.199 ± 1.23
7.139AlaSer: 7.139 ± 0.935
6.799AlaThr: 6.799 ± 0.584
6.459AlaVal: 6.459 ± 0.773
2.805AlaTrp: 2.805 ± 0.433
2.635AlaTyr: 2.635 ± 0.41
0.0AlaXaa: 0.0 ± 0.0
Cys
0.935CysAla: 0.935 ± 0.275
0.17CysCys: 0.17 ± 0.116
0.765CysAsp: 0.765 ± 0.263
0.51CysGlu: 0.51 ± 0.294
0.595CysPhe: 0.595 ± 0.199
0.595CysGly: 0.595 ± 0.242
0.34CysHis: 0.34 ± 0.163
0.255CysIle: 0.255 ± 0.142
0.085CysLys: 0.085 ± 0.092
0.425CysLeu: 0.425 ± 0.217
0.255CysMet: 0.255 ± 0.175
0.425CysAsn: 0.425 ± 0.237
0.765CysPro: 0.765 ± 0.269
0.085CysGln: 0.085 ± 0.07
0.935CysArg: 0.935 ± 0.361
0.425CysSer: 0.425 ± 0.178
0.51CysThr: 0.51 ± 0.243
0.255CysVal: 0.255 ± 0.146
0.255CysTrp: 0.255 ± 0.146
0.255CysTyr: 0.255 ± 0.146
0.0CysXaa: 0.0 ± 0.0
Asp
7.819AspAla: 7.819 ± 0.944
0.17AspCys: 0.17 ± 0.124
3.315AspAsp: 3.315 ± 0.582
3.145AspGlu: 3.145 ± 0.544
1.53AspPhe: 1.53 ± 0.272
6.119AspGly: 6.119 ± 0.896
1.19AspHis: 1.19 ± 0.384
2.635AspIle: 2.635 ± 0.377
1.36AspLys: 1.36 ± 0.337
5.354AspLeu: 5.354 ± 0.673
1.87AspMet: 1.87 ± 0.433
1.445AspAsn: 1.445 ± 0.308
2.975AspPro: 2.975 ± 0.603
2.635AspGln: 2.635 ± 0.513
3.4AspArg: 3.4 ± 0.487
3.57AspSer: 3.57 ± 0.523
3.485AspThr: 3.485 ± 0.507
3.57AspVal: 3.57 ± 0.618
1.02AspTrp: 1.02 ± 0.242
1.36AspTyr: 1.36 ± 0.395
0.0AspXaa: 0.0 ± 0.0
Glu
6.714GluAla: 6.714 ± 0.796
0.51GluCys: 0.51 ± 0.233
2.72GluAsp: 2.72 ± 0.507
2.89GluGlu: 2.89 ± 0.511
1.785GluPhe: 1.785 ± 0.397
3.57GluGly: 3.57 ± 0.489
0.85GluHis: 0.85 ± 0.238
2.89GluIle: 2.89 ± 0.489
2.55GluLys: 2.55 ± 0.496
6.884GluLeu: 6.884 ± 0.683
1.87GluMet: 1.87 ± 0.485
2.21GluAsn: 2.21 ± 0.657
2.72GluPro: 2.72 ± 0.525
3.995GluGln: 3.995 ± 0.582
4.844GluArg: 4.844 ± 0.762
2.72GluSer: 2.72 ± 0.359
3.145GluThr: 3.145 ± 0.462
4.589GluVal: 4.589 ± 0.606
1.19GluTrp: 1.19 ± 0.299
1.7GluTyr: 1.7 ± 0.319
0.0GluXaa: 0.0 ± 0.0
Phe
3.4PheAla: 3.4 ± 0.632
0.34PheCys: 0.34 ± 0.159
2.55PheAsp: 2.55 ± 0.52
1.36PheGlu: 1.36 ± 0.37
0.68PhePhe: 0.68 ± 0.21
2.55PheGly: 2.55 ± 0.403
0.425PheHis: 0.425 ± 0.168
0.85PheIle: 0.85 ± 0.257
0.85PheLys: 0.85 ± 0.235
2.295PheLeu: 2.295 ± 0.401
0.68PheMet: 0.68 ± 0.217
1.19PheAsn: 1.19 ± 0.331
1.275PhePro: 1.275 ± 0.367
1.275PheGln: 1.275 ± 0.303
1.87PheArg: 1.87 ± 0.397
1.36PheSer: 1.36 ± 0.378
1.36PheThr: 1.36 ± 0.35
1.19PheVal: 1.19 ± 0.263
0.255PheTrp: 0.255 ± 0.139
0.85PheTyr: 0.85 ± 0.272
0.0PheXaa: 0.0 ± 0.0
Gly
7.649GlyAla: 7.649 ± 1.065
0.51GlyCys: 0.51 ± 0.191
3.4GlyAsp: 3.4 ± 0.805
4.589GlyGlu: 4.589 ± 0.499
2.805GlyPhe: 2.805 ± 0.439
6.374GlyGly: 6.374 ± 0.664
0.935GlyHis: 0.935 ± 0.258
3.57GlyIle: 3.57 ± 0.486
2.805GlyLys: 2.805 ± 0.517
8.244GlyLeu: 8.244 ± 0.884
1.53GlyMet: 1.53 ± 0.37
2.295GlyAsn: 2.295 ± 0.384
2.72GlyPro: 2.72 ± 0.484
5.099GlyGln: 5.099 ± 0.599
6.714GlyArg: 6.714 ± 0.729
4.589GlySer: 4.589 ± 0.632
3.485GlyThr: 3.485 ± 0.363
4.929GlyVal: 4.929 ± 0.629
1.615GlyTrp: 1.615 ± 0.331
2.125GlyTyr: 2.125 ± 0.506
0.0GlyXaa: 0.0 ± 0.0
His
2.125HisAla: 2.125 ± 0.361
0.085HisCys: 0.085 ± 0.088
0.935HisAsp: 0.935 ± 0.265
0.765HisGlu: 0.765 ± 0.315
0.425HisPhe: 0.425 ± 0.165
1.36HisGly: 1.36 ± 0.373
0.17HisHis: 0.17 ± 0.107
0.935HisIle: 0.935 ± 0.248
0.17HisLys: 0.17 ± 0.122
1.785HisLeu: 1.785 ± 0.397
1.02HisMet: 1.02 ± 0.29
0.85HisAsn: 0.85 ± 0.282
1.445HisPro: 1.445 ± 0.378
1.02HisGln: 1.02 ± 0.375
1.275HisArg: 1.275 ± 0.366
0.68HisSer: 0.68 ± 0.252
0.765HisThr: 0.765 ± 0.254
0.68HisVal: 0.68 ± 0.223
0.255HisTrp: 0.255 ± 0.133
0.765HisTyr: 0.765 ± 0.302
0.0HisXaa: 0.0 ± 0.0
Ile
4.844IleAla: 4.844 ± 0.919
0.595IleCys: 0.595 ± 0.222
3.23IleAsp: 3.23 ± 0.476
2.805IleGlu: 2.805 ± 0.514
0.68IlePhe: 0.68 ± 0.172
2.975IleGly: 2.975 ± 0.423
1.02IleHis: 1.02 ± 0.229
1.785IleIle: 1.785 ± 0.507
1.785IleLys: 1.785 ± 0.433
2.635IleLeu: 2.635 ± 0.44
0.595IleMet: 0.595 ± 0.261
1.02IleAsn: 1.02 ± 0.315
2.125IlePro: 2.125 ± 0.426
1.7IleGln: 1.7 ± 0.465
4.08IleArg: 4.08 ± 0.512
1.87IleSer: 1.87 ± 0.446
3.23IleThr: 3.23 ± 0.528
2.89IleVal: 2.89 ± 0.492
0.51IleTrp: 0.51 ± 0.233
1.105IleTyr: 1.105 ± 0.324
0.0IleXaa: 0.0 ± 0.0
Lys
5.014LysAla: 5.014 ± 0.72
0.085LysCys: 0.085 ± 0.081
0.85LysAsp: 0.85 ± 0.296
1.785LysGlu: 1.785 ± 0.449
0.68LysPhe: 0.68 ± 0.186
2.465LysGly: 2.465 ± 0.31
0.51LysHis: 0.51 ± 0.169
0.935LysIle: 0.935 ± 0.331
1.445LysLys: 1.445 ± 0.387
2.805LysLeu: 2.805 ± 0.554
0.425LysMet: 0.425 ± 0.199
0.935LysAsn: 0.935 ± 0.211
2.21LysPro: 2.21 ± 0.515
1.105LysGln: 1.105 ± 0.274
3.4LysArg: 3.4 ± 0.748
2.21LysSer: 2.21 ± 0.527
2.38LysThr: 2.38 ± 0.45
2.55LysVal: 2.55 ± 0.499
0.255LysTrp: 0.255 ± 0.127
0.68LysTyr: 0.68 ± 0.243
0.0LysXaa: 0.0 ± 0.0
Leu
15.298LeuAla: 15.298 ± 1.405
1.02LeuCys: 1.02 ± 0.266
6.374LeuAsp: 6.374 ± 0.694
7.224LeuGlu: 7.224 ± 0.793
2.295LeuPhe: 2.295 ± 0.498
7.139LeuGly: 7.139 ± 0.6
2.55LeuHis: 2.55 ± 0.495
2.89LeuIle: 2.89 ± 0.442
3.315LeuLys: 3.315 ± 0.679
9.094LeuLeu: 9.094 ± 0.991
1.7LeuMet: 1.7 ± 0.385
3.06LeuAsn: 3.06 ± 0.493
4.505LeuPro: 4.505 ± 0.623
3.57LeuGln: 3.57 ± 0.474
7.479LeuArg: 7.479 ± 0.722
4.589LeuSer: 4.589 ± 0.829
5.439LeuThr: 5.439 ± 0.705
7.479LeuVal: 7.479 ± 0.577
1.19LeuTrp: 1.19 ± 0.312
2.295LeuTyr: 2.295 ± 0.35
0.0LeuXaa: 0.0 ± 0.0
Met
3.995MetAla: 3.995 ± 0.572
0.085MetCys: 0.085 ± 0.095
2.21MetAsp: 2.21 ± 0.429
1.53MetGlu: 1.53 ± 0.377
0.51MetPhe: 0.51 ± 0.196
1.7MetGly: 1.7 ± 0.365
0.425MetHis: 0.425 ± 0.202
0.425MetIle: 0.425 ± 0.175
0.935MetLys: 0.935 ± 0.284
1.36MetLeu: 1.36 ± 0.337
0.51MetMet: 0.51 ± 0.212
0.765MetAsn: 0.765 ± 0.278
1.275MetPro: 1.275 ± 0.384
1.19MetGln: 1.19 ± 0.292
1.445MetArg: 1.445 ± 0.305
1.53MetSer: 1.53 ± 0.344
1.53MetThr: 1.53 ± 0.336
0.51MetVal: 0.51 ± 0.196
0.51MetTrp: 0.51 ± 0.259
0.34MetTyr: 0.34 ± 0.173
0.0MetXaa: 0.0 ± 0.0
Asn
3.315AsnAla: 3.315 ± 0.618
0.085AsnCys: 0.085 ± 0.089
1.275AsnAsp: 1.275 ± 0.32
1.445AsnGlu: 1.445 ± 0.332
0.68AsnPhe: 0.68 ± 0.305
3.145AsnGly: 3.145 ± 0.741
0.68AsnHis: 0.68 ± 0.224
0.935AsnIle: 0.935 ± 0.343
0.595AsnLys: 0.595 ± 0.224
3.145AsnLeu: 3.145 ± 0.553
0.85AsnMet: 0.85 ± 0.263
1.275AsnAsn: 1.275 ± 0.379
2.55AsnPro: 2.55 ± 0.496
1.105AsnGln: 1.105 ± 0.288
2.72AsnArg: 2.72 ± 0.438
2.04AsnSer: 2.04 ± 0.499
0.935AsnThr: 0.935 ± 0.216
1.445AsnVal: 1.445 ± 0.266
0.595AsnTrp: 0.595 ± 0.204
0.765AsnTyr: 0.765 ± 0.233
0.0AsnXaa: 0.0 ± 0.0
Pro
7.394ProAla: 7.394 ± 0.85
0.68ProCys: 0.68 ± 0.297
3.825ProAsp: 3.825 ± 0.61
2.55ProGlu: 2.55 ± 0.417
1.615ProPhe: 1.615 ± 0.406
4.08ProGly: 4.08 ± 0.547
0.85ProHis: 0.85 ± 0.464
1.785ProIle: 1.785 ± 0.31
1.785ProLys: 1.785 ± 0.434
4.589ProLeu: 4.589 ± 0.549
0.765ProMet: 0.765 ± 0.259
1.7ProAsn: 1.7 ± 0.362
2.21ProPro: 2.21 ± 0.481
2.38ProGln: 2.38 ± 0.501
3.315ProArg: 3.315 ± 0.572
3.315ProSer: 3.315 ± 0.402
2.465ProThr: 2.465 ± 0.578
2.975ProVal: 2.975 ± 0.637
0.34ProTrp: 0.34 ± 0.135
1.36ProTyr: 1.36 ± 0.371
0.0ProXaa: 0.0 ± 0.0
Gln
5.524GlnAla: 5.524 ± 1.138
0.255GlnCys: 0.255 ± 0.147
1.87GlnAsp: 1.87 ± 0.396
1.87GlnGlu: 1.87 ± 0.439
1.615GlnPhe: 1.615 ± 0.381
3.57GlnGly: 3.57 ± 0.469
1.105GlnHis: 1.105 ± 0.28
2.55GlnIle: 2.55 ± 0.374
1.02GlnLys: 1.02 ± 0.317
6.459GlnLeu: 6.459 ± 0.619
1.02GlnMet: 1.02 ± 0.349
1.02GlnAsn: 1.02 ± 0.265
2.635GlnPro: 2.635 ± 0.35
3.145GlnGln: 3.145 ± 0.729
3.74GlnArg: 3.74 ± 0.546
2.89GlnSer: 2.89 ± 0.496
2.38GlnThr: 2.38 ± 0.378
4.08GlnVal: 4.08 ± 0.523
0.765GlnTrp: 0.765 ± 0.263
0.765GlnTyr: 0.765 ± 0.312
0.0GlnXaa: 0.0 ± 0.0
Arg
8.669ArgAla: 8.669 ± 1.116
0.765ArgCys: 0.765 ± 0.281
4.42ArgAsp: 4.42 ± 0.546
5.269ArgGlu: 5.269 ± 0.668
2.295ArgPhe: 2.295 ± 0.497
4.165ArgGly: 4.165 ± 0.599
1.7ArgHis: 1.7 ± 0.363
3.825ArgIle: 3.825 ± 0.523
2.295ArgLys: 2.295 ± 0.352
8.414ArgLeu: 8.414 ± 0.823
1.53ArgMet: 1.53 ± 0.304
1.36ArgAsn: 1.36 ± 0.452
3.91ArgPro: 3.91 ± 0.828
4.674ArgGln: 4.674 ± 0.695
6.629ArgArg: 6.629 ± 0.826
4.165ArgSer: 4.165 ± 0.609
3.91ArgThr: 3.91 ± 0.549
4.42ArgVal: 4.42 ± 0.627
1.445ArgTrp: 1.445 ± 0.417
2.975ArgTyr: 2.975 ± 0.449
0.0ArgXaa: 0.0 ± 0.0
Ser
8.329SerAla: 8.329 ± 0.972
0.68SerCys: 0.68 ± 0.249
3.315SerAsp: 3.315 ± 0.674
3.315SerGlu: 3.315 ± 0.438
1.53SerPhe: 1.53 ± 0.271
4.25SerGly: 4.25 ± 0.519
0.935SerHis: 0.935 ± 0.261
2.38SerIle: 2.38 ± 0.459
2.21SerLys: 2.21 ± 0.44
5.099SerLeu: 5.099 ± 1.032
0.68SerMet: 0.68 ± 0.211
1.53SerAsn: 1.53 ± 0.375
3.145SerPro: 3.145 ± 0.546
2.295SerGln: 2.295 ± 0.37
3.23SerArg: 3.23 ± 0.568
3.74SerSer: 3.74 ± 0.599
3.91SerThr: 3.91 ± 0.742
3.315SerVal: 3.315 ± 0.447
1.53SerTrp: 1.53 ± 0.346
1.445SerTyr: 1.445 ± 0.357
0.0SerXaa: 0.0 ± 0.0
Thr
7.479ThrAla: 7.479 ± 0.936
0.51ThrCys: 0.51 ± 0.307
3.315ThrAsp: 3.315 ± 0.55
2.975ThrGlu: 2.975 ± 0.455
1.02ThrPhe: 1.02 ± 0.34
5.354ThrGly: 5.354 ± 0.76
0.68ThrHis: 0.68 ± 0.266
1.87ThrIle: 1.87 ± 0.379
1.7ThrLys: 1.7 ± 0.432
5.949ThrLeu: 5.949 ± 0.667
1.19ThrMet: 1.19 ± 0.307
1.445ThrAsn: 1.445 ± 0.284
2.295ThrPro: 2.295 ± 0.315
1.105ThrGln: 1.105 ± 0.255
3.315ThrArg: 3.315 ± 0.398
3.57ThrSer: 3.57 ± 0.656
4.08ThrThr: 4.08 ± 0.65
6.034ThrVal: 6.034 ± 0.835
0.935ThrTrp: 0.935 ± 0.27
1.615ThrTyr: 1.615 ± 0.331
0.0ThrXaa: 0.0 ± 0.0
Val
7.819ValAla: 7.819 ± 0.849
0.34ValCys: 0.34 ± 0.161
3.23ValAsp: 3.23 ± 0.482
5.099ValGlu: 5.099 ± 0.654
1.615ValPhe: 1.615 ± 0.363
3.995ValGly: 3.995 ± 0.693
0.85ValHis: 0.85 ± 0.242
2.635ValIle: 2.635 ± 0.484
2.295ValLys: 2.295 ± 0.493
5.864ValLeu: 5.864 ± 0.689
1.36ValMet: 1.36 ± 0.371
2.38ValAsn: 2.38 ± 0.424
2.975ValPro: 2.975 ± 0.502
3.655ValGln: 3.655 ± 0.517
4.929ValArg: 4.929 ± 0.584
3.74ValSer: 3.74 ± 0.467
4.25ValThr: 4.25 ± 0.603
4.25ValVal: 4.25 ± 0.708
1.19ValTrp: 1.19 ± 0.265
2.72ValTyr: 2.72 ± 0.5
0.0ValXaa: 0.0 ± 0.0
Trp
1.445TrpAla: 1.445 ± 0.324
0.34TrpCys: 0.34 ± 0.142
0.595TrpAsp: 0.595 ± 0.191
0.935TrpGlu: 0.935 ± 0.262
0.68TrpPhe: 0.68 ± 0.229
0.935TrpGly: 0.935 ± 0.387
0.085TrpHis: 0.085 ± 0.089
1.105TrpIle: 1.105 ± 0.221
0.68TrpLys: 0.68 ± 0.204
1.7TrpLeu: 1.7 ± 0.387
0.935TrpMet: 0.935 ± 0.342
0.425TrpAsn: 0.425 ± 0.189
0.935TrpPro: 0.935 ± 0.326
0.85TrpGln: 0.85 ± 0.203
1.53TrpArg: 1.53 ± 0.37
1.105TrpSer: 1.105 ± 0.305
1.02TrpThr: 1.02 ± 0.321
1.445TrpVal: 1.445 ± 0.392
0.425TrpTrp: 0.425 ± 0.184
0.255TrpTyr: 0.255 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.145TyrAla: 3.145 ± 0.463
0.34TyrCys: 0.34 ± 0.166
1.53TyrAsp: 1.53 ± 0.264
1.36TyrGlu: 1.36 ± 0.31
0.935TyrPhe: 0.935 ± 0.281
2.295TyrGly: 2.295 ± 0.4
0.51TyrHis: 0.51 ± 0.288
0.765TyrIle: 0.765 ± 0.224
0.765TyrLys: 0.765 ± 0.309
2.465TyrLeu: 2.465 ± 0.498
0.51TyrMet: 0.51 ± 0.215
0.935TyrAsn: 0.935 ± 0.288
1.7TyrPro: 1.7 ± 0.441
1.53TyrGln: 1.53 ± 0.368
1.785TyrArg: 1.785 ± 0.326
1.615TyrSer: 1.615 ± 0.398
1.36TyrThr: 1.36 ± 0.373
2.125TyrVal: 2.125 ± 0.444
0.34TyrTrp: 0.34 ± 0.221
0.595TyrTyr: 0.595 ± 0.204
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (11767 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski