Amino acid dipepetide frequency for Pectinobacterium phage PEAT2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.115AlaAla: 10.115 ± 1.182
1.025AlaCys: 1.025 ± 0.307
4.442AlaAsp: 4.442 ± 0.49
4.989AlaGlu: 4.989 ± 0.594
2.939AlaPhe: 2.939 ± 0.497
7.859AlaGly: 7.859 ± 1.199
1.299AlaHis: 1.299 ± 0.327
4.442AlaIle: 4.442 ± 0.557
4.579AlaLys: 4.579 ± 0.648
6.493AlaLeu: 6.493 ± 0.59
2.665AlaMet: 2.665 ± 0.452
3.349AlaAsn: 3.349 ± 0.485
3.075AlaPro: 3.075 ± 0.435
4.442AlaGln: 4.442 ± 0.659
4.989AlaArg: 4.989 ± 0.629
5.741AlaSer: 5.741 ± 0.741
5.741AlaThr: 5.741 ± 0.651
6.971AlaVal: 6.971 ± 0.756
1.23AlaTrp: 1.23 ± 0.305
3.007AlaTyr: 3.007 ± 0.405
0.0AlaXaa: 0.0 ± 0.0
Cys
1.23CysAla: 1.23 ± 0.287
0.478CysCys: 0.478 ± 0.244
0.957CysAsp: 0.957 ± 0.255
1.299CysGlu: 1.299 ± 0.284
0.683CysPhe: 0.683 ± 0.24
0.82CysGly: 0.82 ± 0.267
0.615CysHis: 0.615 ± 0.249
0.888CysIle: 0.888 ± 0.23
0.957CysLys: 0.957 ± 0.344
1.025CysLeu: 1.025 ± 0.227
0.205CysMet: 0.205 ± 0.116
0.888CysAsn: 0.888 ± 0.205
0.683CysPro: 0.683 ± 0.175
0.547CysGln: 0.547 ± 0.16
0.957CysArg: 0.957 ± 0.211
1.162CysSer: 1.162 ± 0.269
1.162CysThr: 1.162 ± 0.279
0.888CysVal: 0.888 ± 0.345
0.273CysTrp: 0.273 ± 0.143
0.615CysTyr: 0.615 ± 0.233
0.0CysXaa: 0.0 ± 0.0
Asp
6.903AspAla: 6.903 ± 0.651
0.547AspCys: 0.547 ± 0.156
3.486AspAsp: 3.486 ± 0.46
3.691AspGlu: 3.691 ± 0.543
2.46AspPhe: 2.46 ± 0.405
6.083AspGly: 6.083 ± 0.687
0.683AspHis: 0.683 ± 0.211
4.306AspIle: 4.306 ± 0.5
2.255AspLys: 2.255 ± 0.472
3.075AspLeu: 3.075 ± 0.44
1.845AspMet: 1.845 ± 0.428
2.87AspAsn: 2.87 ± 0.453
2.665AspPro: 2.665 ± 0.361
1.162AspGln: 1.162 ± 0.213
2.734AspArg: 2.734 ± 0.48
4.511AspSer: 4.511 ± 0.433
3.896AspThr: 3.896 ± 0.446
5.057AspVal: 5.057 ± 0.527
1.504AspTrp: 1.504 ± 0.319
1.367AspTyr: 1.367 ± 0.299
0.0AspXaa: 0.0 ± 0.0
Glu
4.716GluAla: 4.716 ± 0.59
0.82GluCys: 0.82 ± 0.229
2.665GluAsp: 2.665 ± 0.444
2.665GluGlu: 2.665 ± 0.4
1.982GluPhe: 1.982 ± 0.498
2.255GluGly: 2.255 ± 0.327
0.888GluHis: 0.888 ± 0.227
3.28GluIle: 3.28 ± 0.414
2.392GluLys: 2.392 ± 0.44
5.331GluLeu: 5.331 ± 0.709
1.64GluMet: 1.64 ± 0.288
2.119GluAsn: 2.119 ± 0.368
1.709GluPro: 1.709 ± 0.357
3.486GluGln: 3.486 ± 0.608
2.734GluArg: 2.734 ± 0.358
2.802GluSer: 2.802 ± 0.391
2.597GluThr: 2.597 ± 0.389
3.007GluVal: 3.007 ± 0.43
1.367GluTrp: 1.367 ± 0.325
2.255GluTyr: 2.255 ± 0.365
0.0GluXaa: 0.0 ± 0.0
Phe
2.87PheAla: 2.87 ± 0.424
0.82PheCys: 0.82 ± 0.18
2.734PheAsp: 2.734 ± 0.391
1.845PheGlu: 1.845 ± 0.372
1.299PhePhe: 1.299 ± 0.365
3.486PheGly: 3.486 ± 0.53
0.752PheHis: 0.752 ± 0.205
2.46PheIle: 2.46 ± 0.391
1.504PheLys: 1.504 ± 0.266
2.392PheLeu: 2.392 ± 0.451
0.82PheMet: 0.82 ± 0.274
2.597PheAsn: 2.597 ± 0.437
1.572PhePro: 1.572 ± 0.292
0.82PheGln: 0.82 ± 0.228
1.914PheArg: 1.914 ± 0.395
2.529PheSer: 2.529 ± 0.418
2.529PheThr: 2.529 ± 0.39
2.529PheVal: 2.529 ± 0.383
0.478PheTrp: 0.478 ± 0.176
1.299PheTyr: 1.299 ± 0.277
0.0PheXaa: 0.0 ± 0.0
Gly
5.878GlyAla: 5.878 ± 0.836
0.752GlyCys: 0.752 ± 0.217
5.057GlyAsp: 5.057 ± 0.544
4.442GlyGlu: 4.442 ± 0.503
1.914GlyPhe: 1.914 ± 0.407
6.629GlyGly: 6.629 ± 1.623
1.914GlyHis: 1.914 ± 0.488
4.032GlyIle: 4.032 ± 0.543
4.169GlyLys: 4.169 ± 0.535
4.921GlyLeu: 4.921 ± 0.536
2.324GlyMet: 2.324 ± 0.381
2.87GlyAsn: 2.87 ± 0.481
2.187GlyPro: 2.187 ± 0.377
2.939GlyGln: 2.939 ± 0.395
3.417GlyArg: 3.417 ± 0.392
5.467GlySer: 5.467 ± 0.897
5.194GlyThr: 5.194 ± 0.887
6.219GlyVal: 6.219 ± 0.587
1.504GlyTrp: 1.504 ± 0.307
2.734GlyTyr: 2.734 ± 0.433
0.0GlyXaa: 0.0 ± 0.0
His
1.914HisAla: 1.914 ± 0.388
0.683HisCys: 0.683 ± 0.19
1.093HisAsp: 1.093 ± 0.231
0.683HisGlu: 0.683 ± 0.227
0.82HisPhe: 0.82 ± 0.239
1.504HisGly: 1.504 ± 0.366
0.888HisHis: 0.888 ± 0.227
1.709HisIle: 1.709 ± 0.361
0.888HisLys: 0.888 ± 0.227
1.093HisLeu: 1.093 ± 0.22
0.273HisMet: 0.273 ± 0.126
0.82HisAsn: 0.82 ± 0.233
1.093HisPro: 1.093 ± 0.257
0.547HisGln: 0.547 ± 0.204
1.093HisArg: 1.093 ± 0.271
1.367HisSer: 1.367 ± 0.311
0.683HisThr: 0.683 ± 0.205
1.504HisVal: 1.504 ± 0.331
0.547HisTrp: 0.547 ± 0.187
0.683HisTyr: 0.683 ± 0.233
0.0HisXaa: 0.0 ± 0.0
Ile
5.536IleAla: 5.536 ± 0.63
1.162IleCys: 1.162 ± 0.257
3.691IleAsp: 3.691 ± 0.48
3.759IleGlu: 3.759 ± 0.551
1.709IlePhe: 1.709 ± 0.29
3.759IleGly: 3.759 ± 0.495
1.093IleHis: 1.093 ± 0.274
2.734IleIle: 2.734 ± 0.498
3.212IleLys: 3.212 ± 0.457
3.691IleLeu: 3.691 ± 0.558
1.572IleMet: 1.572 ± 0.32
3.896IleAsn: 3.896 ± 0.643
2.529IlePro: 2.529 ± 0.438
1.777IleGln: 1.777 ± 0.386
3.417IleArg: 3.417 ± 0.504
3.417IleSer: 3.417 ± 0.552
4.169IleThr: 4.169 ± 0.417
3.759IleVal: 3.759 ± 0.459
1.025IleTrp: 1.025 ± 0.26
1.504IleTyr: 1.504 ± 0.345
0.0IleXaa: 0.0 ± 0.0
Lys
4.784LysAla: 4.784 ± 0.682
0.41LysCys: 0.41 ± 0.161
3.417LysAsp: 3.417 ± 0.442
1.367LysGlu: 1.367 ± 0.257
2.324LysPhe: 2.324 ± 0.33
2.597LysGly: 2.597 ± 0.384
0.683LysHis: 0.683 ± 0.183
2.392LysIle: 2.392 ± 0.496
2.255LysLys: 2.255 ± 0.403
4.306LysLeu: 4.306 ± 0.554
1.982LysMet: 1.982 ± 0.318
2.392LysAsn: 2.392 ± 0.368
1.845LysPro: 1.845 ± 0.29
2.119LysGln: 2.119 ± 0.421
3.554LysArg: 3.554 ± 0.501
3.349LysSer: 3.349 ± 0.515
3.212LysThr: 3.212 ± 0.41
3.007LysVal: 3.007 ± 0.516
0.752LysTrp: 0.752 ± 0.218
0.957LysTyr: 0.957 ± 0.234
0.0LysXaa: 0.0 ± 0.0
Leu
5.536LeuAla: 5.536 ± 0.814
1.23LeuCys: 1.23 ± 0.281
6.356LeuAsp: 6.356 ± 0.712
3.349LeuGlu: 3.349 ± 0.438
3.075LeuPhe: 3.075 ± 0.394
4.784LeuGly: 4.784 ± 0.517
1.914LeuHis: 1.914 ± 0.345
4.647LeuIle: 4.647 ± 0.562
3.691LeuLys: 3.691 ± 0.481
5.262LeuLeu: 5.262 ± 0.642
1.777LeuMet: 1.777 ± 0.317
3.759LeuAsn: 3.759 ± 0.589
2.87LeuPro: 2.87 ± 0.464
2.597LeuGln: 2.597 ± 0.378
4.852LeuArg: 4.852 ± 0.548
4.921LeuSer: 4.921 ± 0.553
6.014LeuThr: 6.014 ± 0.534
4.579LeuVal: 4.579 ± 0.559
1.093LeuTrp: 1.093 ± 0.257
2.392LeuTyr: 2.392 ± 0.365
0.0LeuXaa: 0.0 ± 0.0
Met
1.982MetAla: 1.982 ± 0.468
0.82MetCys: 0.82 ± 0.253
1.025MetAsp: 1.025 ± 0.297
1.093MetGlu: 1.093 ± 0.225
1.435MetPhe: 1.435 ± 0.268
1.504MetGly: 1.504 ± 0.312
0.41MetHis: 0.41 ± 0.182
1.367MetIle: 1.367 ± 0.3
1.025MetLys: 1.025 ± 0.255
2.05MetLeu: 2.05 ± 0.412
1.367MetMet: 1.367 ± 0.252
1.299MetAsn: 1.299 ± 0.316
0.752MetPro: 0.752 ± 0.222
0.752MetGln: 0.752 ± 0.182
2.802MetArg: 2.802 ± 0.577
2.802MetSer: 2.802 ± 0.427
2.87MetThr: 2.87 ± 0.386
1.845MetVal: 1.845 ± 0.315
0.205MetTrp: 0.205 ± 0.097
1.162MetTyr: 1.162 ± 0.227
0.0MetXaa: 0.0 ± 0.0
Asn
4.716AsnAla: 4.716 ± 0.559
0.888AsnCys: 0.888 ± 0.216
2.46AsnAsp: 2.46 ± 0.361
2.597AsnGlu: 2.597 ± 0.341
1.777AsnPhe: 1.777 ± 0.366
3.759AsnGly: 3.759 ± 0.463
0.957AsnHis: 0.957 ± 0.235
2.802AsnIle: 2.802 ± 0.374
1.845AsnLys: 1.845 ± 0.363
3.827AsnLeu: 3.827 ± 0.497
1.093AsnMet: 1.093 ± 0.247
3.075AsnAsn: 3.075 ± 0.602
3.075AsnPro: 3.075 ± 0.486
1.367AsnGln: 1.367 ± 0.283
1.982AsnArg: 1.982 ± 0.343
3.212AsnSer: 3.212 ± 0.535
2.597AsnThr: 2.597 ± 0.417
4.169AsnVal: 4.169 ± 0.554
0.752AsnTrp: 0.752 ± 0.202
1.572AsnTyr: 1.572 ± 0.302
0.0AsnXaa: 0.0 ± 0.0
Pro
4.511ProAla: 4.511 ± 0.483
0.957ProCys: 0.957 ± 0.254
2.939ProAsp: 2.939 ± 0.469
2.187ProGlu: 2.187 ± 0.354
0.888ProPhe: 0.888 ± 0.231
3.827ProGly: 3.827 ± 0.606
0.683ProHis: 0.683 ± 0.193
1.845ProIle: 1.845 ± 0.339
1.504ProLys: 1.504 ± 0.296
3.144ProLeu: 3.144 ± 0.471
0.478ProMet: 0.478 ± 0.143
2.529ProAsn: 2.529 ± 0.349
2.255ProPro: 2.255 ± 0.589
1.23ProGln: 1.23 ± 0.229
2.802ProArg: 2.802 ± 0.354
2.255ProSer: 2.255 ± 0.419
2.529ProThr: 2.529 ± 0.466
4.989ProVal: 4.989 ± 0.605
0.82ProTrp: 0.82 ± 0.238
1.162ProTyr: 1.162 ± 0.247
0.0ProXaa: 0.0 ± 0.0
Gln
2.529GlnAla: 2.529 ± 0.565
0.683GlnCys: 0.683 ± 0.171
1.572GlnAsp: 1.572 ± 0.309
2.255GlnGlu: 2.255 ± 0.323
1.777GlnPhe: 1.777 ± 0.318
2.324GlnGly: 2.324 ± 0.36
1.025GlnHis: 1.025 ± 0.217
2.392GlnIle: 2.392 ± 0.406
1.435GlnLys: 1.435 ± 0.356
3.827GlnLeu: 3.827 ± 0.521
1.435GlnMet: 1.435 ± 0.347
1.162GlnAsn: 1.162 ± 0.295
1.504GlnPro: 1.504 ± 0.312
2.734GlnGln: 2.734 ± 0.471
2.05GlnArg: 2.05 ± 0.373
2.05GlnSer: 2.05 ± 0.442
2.665GlnThr: 2.665 ± 0.426
2.734GlnVal: 2.734 ± 0.397
0.478GlnTrp: 0.478 ± 0.17
1.572GlnTyr: 1.572 ± 0.277
0.0GlnXaa: 0.0 ± 0.0
Arg
3.554ArgAla: 3.554 ± 0.49
1.367ArgCys: 1.367 ± 0.323
2.324ArgAsp: 2.324 ± 0.381
3.075ArgGlu: 3.075 ± 0.492
2.529ArgPhe: 2.529 ± 0.421
2.597ArgGly: 2.597 ± 0.474
1.572ArgHis: 1.572 ± 0.348
3.964ArgIle: 3.964 ± 0.394
3.075ArgLys: 3.075 ± 0.418
5.262ArgLeu: 5.262 ± 0.581
1.572ArgMet: 1.572 ± 0.351
2.46ArgAsn: 2.46 ± 0.373
2.597ArgPro: 2.597 ± 0.463
2.597ArgGln: 2.597 ± 0.482
3.964ArgArg: 3.964 ± 0.735
3.075ArgSer: 3.075 ± 0.393
3.007ArgThr: 3.007 ± 0.339
4.237ArgVal: 4.237 ± 0.578
1.299ArgTrp: 1.299 ± 0.392
1.845ArgTyr: 1.845 ± 0.304
0.0ArgXaa: 0.0 ± 0.0
Ser
5.741SerAla: 5.741 ± 0.718
0.683SerCys: 0.683 ± 0.217
4.169SerAsp: 4.169 ± 0.435
1.845SerGlu: 1.845 ± 0.35
2.734SerPhe: 2.734 ± 0.462
5.331SerGly: 5.331 ± 0.655
1.299SerHis: 1.299 ± 0.339
3.691SerIle: 3.691 ± 0.47
4.032SerLys: 4.032 ± 0.565
5.809SerLeu: 5.809 ± 0.614
2.119SerMet: 2.119 ± 0.411
3.007SerAsn: 3.007 ± 0.418
2.734SerPro: 2.734 ± 0.451
2.734SerGln: 2.734 ± 0.36
2.529SerArg: 2.529 ± 0.404
4.101SerSer: 4.101 ± 0.72
4.306SerThr: 4.306 ± 0.487
5.126SerVal: 5.126 ± 0.623
0.547SerTrp: 0.547 ± 0.19
2.119SerTyr: 2.119 ± 0.4
0.0SerXaa: 0.0 ± 0.0
Thr
5.672ThrAla: 5.672 ± 0.831
1.025ThrCys: 1.025 ± 0.269
4.921ThrAsp: 4.921 ± 0.603
3.007ThrGlu: 3.007 ± 0.466
2.324ThrPhe: 2.324 ± 0.386
6.288ThrGly: 6.288 ± 0.611
1.504ThrHis: 1.504 ± 0.454
3.896ThrIle: 3.896 ± 0.433
2.529ThrLys: 2.529 ± 0.398
5.467ThrLeu: 5.467 ± 0.618
1.777ThrMet: 1.777 ± 0.304
3.007ThrAsn: 3.007 ± 0.374
4.374ThrPro: 4.374 ± 0.607
2.187ThrGln: 2.187 ± 0.433
3.007ThrArg: 3.007 ± 0.39
3.759ThrSer: 3.759 ± 0.585
4.032ThrThr: 4.032 ± 0.621
6.356ThrVal: 6.356 ± 0.696
1.093ThrTrp: 1.093 ± 0.324
1.23ThrTyr: 1.23 ± 0.266
0.0ThrXaa: 0.0 ± 0.0
Val
7.313ValAla: 7.313 ± 0.752
1.025ValCys: 1.025 ± 0.249
4.647ValAsp: 4.647 ± 0.623
4.716ValGlu: 4.716 ± 0.646
2.665ValPhe: 2.665 ± 0.525
5.536ValGly: 5.536 ± 0.584
0.957ValHis: 0.957 ± 0.231
4.374ValIle: 4.374 ± 0.512
3.486ValLys: 3.486 ± 0.357
3.896ValLeu: 3.896 ± 0.547
2.324ValMet: 2.324 ± 0.354
4.579ValAsn: 4.579 ± 0.519
3.759ValPro: 3.759 ± 0.495
2.05ValGln: 2.05 ± 0.387
3.896ValArg: 3.896 ± 0.535
4.511ValSer: 4.511 ± 0.496
6.903ValThr: 6.903 ± 0.665
4.101ValVal: 4.101 ± 0.568
1.093ValTrp: 1.093 ± 0.288
3.144ValTyr: 3.144 ± 0.398
0.0ValXaa: 0.0 ± 0.0
Trp
0.957TrpAla: 0.957 ± 0.29
0.41TrpCys: 0.41 ± 0.18
1.093TrpAsp: 1.093 ± 0.262
0.273TrpGlu: 0.273 ± 0.231
0.752TrpPhe: 0.752 ± 0.241
1.025TrpGly: 1.025 ± 0.234
0.547TrpHis: 0.547 ± 0.171
0.82TrpIle: 0.82 ± 0.224
1.23TrpLys: 1.23 ± 0.28
2.05TrpLeu: 2.05 ± 0.44
0.273TrpMet: 0.273 ± 0.125
0.41TrpAsn: 0.41 ± 0.237
0.547TrpPro: 0.547 ± 0.222
0.683TrpGln: 0.683 ± 0.267
0.82TrpArg: 0.82 ± 0.232
1.504TrpSer: 1.504 ± 0.257
0.957TrpThr: 0.957 ± 0.213
1.367TrpVal: 1.367 ± 0.252
0.205TrpTrp: 0.205 ± 0.117
0.752TrpTyr: 0.752 ± 0.206
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.939TyrAla: 2.939 ± 0.473
0.547TyrCys: 0.547 ± 0.197
2.05TyrAsp: 2.05 ± 0.298
1.64TyrGlu: 1.64 ± 0.374
1.162TyrPhe: 1.162 ± 0.253
2.597TyrGly: 2.597 ± 0.387
0.205TyrHis: 0.205 ± 0.117
1.367TyrIle: 1.367 ± 0.324
1.64TyrLys: 1.64 ± 0.348
1.777TyrLeu: 1.777 ± 0.333
0.957TyrMet: 0.957 ± 0.246
1.435TyrAsn: 1.435 ± 0.311
1.709TyrPro: 1.709 ± 0.361
1.435TyrGln: 1.435 ± 0.284
2.46TyrArg: 2.46 ± 0.513
2.119TyrSer: 2.119 ± 0.307
2.392TyrThr: 2.392 ± 0.326
2.529TyrVal: 2.529 ± 0.394
0.41TyrTrp: 0.41 ± 0.172
1.025TyrTyr: 1.025 ± 0.265
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (14633 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski