Spathaspora sp. JA1

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Debaryomycetaceae; Spathaspora; unclassified Spathaspora

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5246 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A421JR32|A0A421JR32_9ASCO Coatomer subunit alpha OS=Spathaspora sp. JA1 OX=2028339 GN=JA1_002214 PE=4 SV=1
MM1 pKa = 7.46EE2 pKa = 5.6GKK4 pKa = 10.44LEE6 pKa = 3.99EE7 pKa = 5.09DD8 pKa = 3.02KK9 pKa = 11.59LEE11 pKa = 4.24EE12 pKa = 4.14VKK14 pKa = 10.75HH15 pKa = 4.84EE16 pKa = 4.16QGHH19 pKa = 5.24KK20 pKa = 9.66VWDD23 pKa = 4.29GLDD26 pKa = 3.31HH27 pKa = 7.02SLTSNLLDD35 pKa = 4.71KK36 pKa = 9.82ITNIIEE42 pKa = 4.11CAICSEE48 pKa = 3.95IMIIPVTAEE57 pKa = 4.11CGHH60 pKa = 5.81SFCYY64 pKa = 9.99GCIYY68 pKa = 10.53QWFGTKK74 pKa = 10.19LNCPTCRR81 pKa = 11.84KK82 pKa = 10.29VIDD85 pKa = 4.46HH86 pKa = 6.75KK87 pKa = 11.02PILNIQLKK95 pKa = 9.21EE96 pKa = 3.81ISKK99 pKa = 10.7GMVDD103 pKa = 5.06LLIDD107 pKa = 3.77TDD109 pKa = 4.43SKK111 pKa = 11.86DD112 pKa = 3.45KK113 pKa = 11.18LHH115 pKa = 7.17LIGIRR120 pKa = 11.84DD121 pKa = 3.59EE122 pKa = 4.56SIKK125 pKa = 11.03SFEE128 pKa = 4.14NDD130 pKa = 3.3KK131 pKa = 10.9SCKK134 pKa = 9.99QIFGNLFKK142 pKa = 10.85GAVMLIDD149 pKa = 4.09NSDD152 pKa = 3.37GVPRR156 pKa = 11.84CGNCHH161 pKa = 5.9WEE163 pKa = 3.84AHH165 pKa = 5.99GNVCNHH171 pKa = 6.54CGTRR175 pKa = 11.84FRR177 pKa = 11.84NARR180 pKa = 11.84NMVDD184 pKa = 3.59GDD186 pKa = 3.87FDD188 pKa = 6.02GDD190 pKa = 4.26DD191 pKa = 4.13DD192 pKa = 5.73EE193 pKa = 6.29EE194 pKa = 6.0DD195 pKa = 3.63LFQDD199 pKa = 3.08VDD201 pKa = 3.99SEE203 pKa = 4.17NDD205 pKa = 3.01YY206 pKa = 11.56DD207 pKa = 5.18FEE209 pKa = 7.08DD210 pKa = 4.47GFIDD214 pKa = 3.83GRR216 pKa = 11.84TVQAIEE222 pKa = 4.44RR223 pKa = 11.84LVEE226 pKa = 4.33TYY228 pKa = 10.9GSDD231 pKa = 3.34LDD233 pKa = 5.1LYY235 pKa = 10.71EE236 pKa = 6.36DD237 pKa = 4.54PDD239 pKa = 4.05EE240 pKa = 5.13DD241 pKa = 4.99LSPSANDD248 pKa = 2.7NWFGFEE254 pKa = 4.34TDD256 pKa = 3.96DD257 pKa = 4.82SSSFEE262 pKa = 4.43LNGEE266 pKa = 4.14DD267 pKa = 5.21EE268 pKa = 5.03EE269 pKa = 6.83DD270 pKa = 3.84DD271 pKa = 4.22LSPSVIEE278 pKa = 5.13AMHH281 pKa = 6.38EE282 pKa = 3.89LHH284 pKa = 6.85NDD286 pKa = 3.49DD287 pKa = 4.23GSGSEE292 pKa = 4.21EE293 pKa = 4.68YY294 pKa = 10.75YY295 pKa = 10.75DD296 pKa = 3.56EE297 pKa = 6.68HH298 pKa = 7.47EE299 pKa = 4.63DD300 pKa = 3.33RR301 pKa = 11.84QEE303 pKa = 3.77FVEE306 pKa = 4.12EE307 pKa = 4.52HH308 pKa = 6.74EE309 pKa = 5.24DD310 pKa = 4.07GEE312 pKa = 4.9DD313 pKa = 3.36VHH315 pKa = 8.58GDD317 pKa = 3.24NDD319 pKa = 3.89GDD321 pKa = 3.72GGYY324 pKa = 9.79SEE326 pKa = 5.68DD327 pKa = 4.46HH328 pKa = 6.61NEE330 pKa = 4.26DD331 pKa = 4.27YY332 pKa = 11.18YY333 pKa = 11.63DD334 pKa = 4.1SGPGDD339 pKa = 3.47YY340 pKa = 10.7HH341 pKa = 7.51GDD343 pKa = 3.66SGQEE347 pKa = 4.07YY348 pKa = 8.96YY349 pKa = 10.93DD350 pKa = 4.07GDD352 pKa = 3.56NGYY355 pKa = 10.94DD356 pKa = 4.04DD357 pKa = 5.45GDD359 pKa = 3.47NGYY362 pKa = 10.85DD363 pKa = 4.18DD364 pKa = 5.36GGYY367 pKa = 10.66DD368 pKa = 4.41DD369 pKa = 5.98GGYY372 pKa = 10.51DD373 pKa = 5.18DD374 pKa = 4.14QEE376 pKa = 4.26SWW378 pKa = 3.2

Molecular weight: 42.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A421JM15|A0A421JM15_9ASCO Uncharacterized protein OS=Spathaspora sp. JA1 OX=2028339 GN=JA1_003636 PE=4 SV=1
MM1 pKa = 7.14YY2 pKa = 10.41KK3 pKa = 10.44VGITGKK9 pKa = 10.19FGVRR13 pKa = 11.84YY14 pKa = 9.36GSSLRR19 pKa = 11.84RR20 pKa = 11.84QTKK23 pKa = 9.43KK24 pKa = 11.15LEE26 pKa = 4.21VQQHH30 pKa = 5.25AKK32 pKa = 9.97YY33 pKa = 10.58DD34 pKa = 3.58CSFCGKK40 pKa = 8.86RR41 pKa = 11.84TVQRR45 pKa = 11.84GAAGIWNCKK54 pKa = 7.04SCRR57 pKa = 11.84KK58 pKa = 7.41TVAGGAYY65 pKa = 8.32TVSTAAAATVRR76 pKa = 11.84STIRR80 pKa = 11.84RR81 pKa = 11.84LRR83 pKa = 11.84DD84 pKa = 3.06MAEE87 pKa = 3.6AA88 pKa = 4.27

Molecular weight: 9.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5246

0

5246

2781732

51

4819

530.3

59.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.1 ± 0.033

1.1 ± 0.01

5.788 ± 0.024

6.873 ± 0.04

4.431 ± 0.022

5.22 ± 0.031

2.102 ± 0.012

7.234 ± 0.029

6.857 ± 0.035

9.292 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.811 ± 0.012

5.843 ± 0.024

4.708 ± 0.036

4.538 ± 0.028

3.993 ± 0.022

8.594 ± 0.042

6.18 ± 0.047

5.734 ± 0.024

1.007 ± 0.011

3.594 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski