Streptococcus phage Javan270

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B0N3|A0A4D6B0N3_9CAUD Holin OS=Streptococcus phage Javan270 OX=2548085 GN=Javan270_0004 PE=4 SV=1
MM1 pKa = 7.32IHH3 pKa = 6.76HH4 pKa = 7.27LLVDD8 pKa = 4.07ALGEE12 pKa = 4.09LDD14 pKa = 5.5DD15 pKa = 5.15PYY17 pKa = 11.46EE18 pKa = 4.05FNYY21 pKa = 10.47LNFMEE26 pKa = 4.68YY27 pKa = 11.15YY28 pKa = 9.67NLKK31 pKa = 7.61TTTEE35 pKa = 3.81EE36 pKa = 4.13VMVKK40 pKa = 10.36EE41 pKa = 4.82EE42 pKa = 4.02YY43 pKa = 9.97QALLNFNKK51 pKa = 10.36GQFYY55 pKa = 11.17EE56 pKa = 4.25EE57 pKa = 5.21DD58 pKa = 3.74NNIDD62 pKa = 3.98DD63 pKa = 4.29ATTSDD68 pKa = 4.04FLSSCVV74 pKa = 3.27

Molecular weight: 8.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AWD2|A0A4D6AWD2_9CAUD Tail length tape-measure protein OS=Streptococcus phage Javan270 OX=2548085 GN=Javan270_0011 PE=4 SV=1
MM1 pKa = 7.41NKK3 pKa = 9.86EE4 pKa = 3.28DD5 pKa = 3.75WLRR8 pKa = 11.84LLEE11 pKa = 4.17KK12 pKa = 10.49AIDD15 pKa = 4.38NIPEE19 pKa = 4.14TVTAIASLVTAITVARR35 pKa = 11.84QNKK38 pKa = 7.99KK39 pKa = 10.16RR40 pKa = 11.84KK41 pKa = 8.4PEE43 pKa = 3.67SRR45 pKa = 11.84KK46 pKa = 10.12RR47 pKa = 11.84KK48 pKa = 9.02GKK50 pKa = 10.09RR51 pKa = 2.92

Molecular weight: 5.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

10112

39

1277

183.9

20.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.636 ± 0.609

0.554 ± 0.086

6.379 ± 0.299

7.991 ± 0.436

4.45 ± 0.3

6.092 ± 0.355

1.513 ± 0.136

7.031 ± 0.3

8.574 ± 0.356

8.871 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.158

5.696 ± 0.259

2.571 ± 0.208

4.055 ± 0.245

4.233 ± 0.257

6.349 ± 0.309

5.726 ± 0.311

5.716 ± 0.309

1.295 ± 0.153

3.758 ± 0.346
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski