Klebsiella phage 117

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Przondovirus; unclassified Przondovirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WCH4|A0A5Q2WCH4_9CAUD Tail tubular protein A OS=Klebsiella phage 117 OX=2601666 PE=4 SV=1
MM1 pKa = 7.38NMQDD5 pKa = 4.11AYY7 pKa = 10.62FGSAAEE13 pKa = 4.04LDD15 pKa = 4.97AINEE19 pKa = 3.9MLAAIGEE26 pKa = 4.7SPVTTLDD33 pKa = 3.33EE34 pKa = 5.18DD35 pKa = 4.25GSADD39 pKa = 3.27VANARR44 pKa = 11.84RR45 pKa = 11.84ILNRR49 pKa = 11.84INRR52 pKa = 11.84QIQSKK57 pKa = 9.61GWAFNINQSATLTPDD72 pKa = 3.44ADD74 pKa = 3.61TGLIPFRR81 pKa = 11.84PAYY84 pKa = 10.44LSILGGQYY92 pKa = 10.17VNRR95 pKa = 11.84GGWVYY100 pKa = 11.26DD101 pKa = 3.54KK102 pKa = 10.9STDD105 pKa = 3.07SDD107 pKa = 4.07TFSGPITVTLITLQDD122 pKa = 3.68YY123 pKa = 11.66DD124 pKa = 4.32EE125 pKa = 4.72MPEE128 pKa = 5.08CFRR131 pKa = 11.84QWIVTKK137 pKa = 10.66ASRR140 pKa = 11.84QFNSRR145 pKa = 11.84FFGAEE150 pKa = 3.73DD151 pKa = 3.76VEE153 pKa = 4.46NSLAQEE159 pKa = 3.88EE160 pKa = 4.75MEE162 pKa = 5.66ARR164 pKa = 11.84MACNEE169 pKa = 3.84YY170 pKa = 10.91EE171 pKa = 4.14MDD173 pKa = 3.84FGQYY177 pKa = 11.01NMLDD181 pKa = 2.92GDD183 pKa = 4.42AFVQGLIGRR192 pKa = 4.47

Molecular weight: 21.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W9Q0|A0A5Q2W9Q0_9CAUD Uncharacterized protein OS=Klebsiella phage 117 OX=2601666 PE=4 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84LIRR5 pKa = 11.84THH7 pKa = 5.27YY8 pKa = 9.71RR9 pKa = 11.84RR10 pKa = 11.84HH11 pKa = 5.0QMSINLILIIVFILAAIVWSMNDD34 pKa = 3.87EE35 pKa = 4.3PPKK38 pKa = 10.61GAA40 pKa = 4.39

Molecular weight: 4.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

12252

40

1321

260.7

29.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.076 ± 0.512

1.012 ± 0.168

6.334 ± 0.178

7.011 ± 0.547

3.567 ± 0.194

7.819 ± 0.391

1.763 ± 0.216

4.807 ± 0.148

6.579 ± 0.484

8.129 ± 0.309

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.571 ± 0.207

4.261 ± 0.337

3.689 ± 0.181

4.285 ± 0.337

5.452 ± 0.243

6.072 ± 0.404

5.624 ± 0.337

6.954 ± 0.421

1.461 ± 0.209

3.534 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski