Taphrina deformans (strain PYCC 5710 / ATCC 11124 / CBS 356.35 / IMI 108563 / JCM 9778 / NBRC 8474) (Peach leaf curl fungus) (Lalaria deformans)
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4659 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4XC33|R4XC33_TAPDE Uncharacterized protein OS=Taphrina deformans (strain PYCC 5710 / ATCC 11124 / CBS 356.35 / IMI 108563 / JCM 9778 / NBRC 8474) OX=1097556 GN=TAPDE_003651 PE=4 SV=1
MM1 pKa = 7.52 SNFYY5 pKa = 11.15 DD6 pKa = 3.59 EE7 pKa = 5.73 VEE9 pKa = 4.11 IEE11 pKa = 5.23 DD12 pKa = 3.58 MSFDD16 pKa = 3.79 EE17 pKa = 5.59 KK18 pKa = 10.88 KK19 pKa = 10.33 QLYY22 pKa = 9.66 HH23 pKa = 6.57 FPCPCGDD30 pKa = 3.22 RR31 pKa = 11.84 FEE33 pKa = 4.21 ITIAQLKK40 pKa = 9.84 EE41 pKa = 3.8 GDD43 pKa = 3.94 DD44 pKa = 3.81 VARR47 pKa = 11.84 CPSCSLIILVIYY59 pKa = 10.25 DD60 pKa = 3.26 QDD62 pKa = 4.13 DD63 pKa = 3.87 YY64 pKa = 11.86 LSEE67 pKa = 4.16 EE68 pKa = 4.21 EE69 pKa = 4.4 EE70 pKa = 4.4 EE71 pKa = 4.28 EE72 pKa = 4.37 EE73 pKa = 4.49 EE74 pKa = 4.38 AVPLAAQPSAIAVV87 pKa = 3.58
Molecular weight: 9.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.785
IPC2_protein 3.795
IPC_protein 3.719
Toseland 3.541
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.592
Rodwell 3.554
Grimsley 3.452
Solomon 3.681
Lehninger 3.63
Nozaki 3.821
DTASelect 3.961
Thurlkill 3.579
EMBOSS 3.617
Sillero 3.834
Patrickios 0.54
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.774
Protein with the highest isoelectric point:
>tr|R4XLQ6|R4XLQ6_TAPDE Uncharacterized protein OS=Taphrina deformans (strain PYCC 5710 / ATCC 11124 / CBS 356.35 / IMI 108563 / JCM 9778 / NBRC 8474) OX=1097556 GN=TAPDE_004638 PE=3 SV=1
MM1 pKa = 7.23 RR2 pKa = 11.84 RR3 pKa = 11.84 CWNSGTWTRR12 pKa = 11.84 TSRR15 pKa = 11.84 SRR17 pKa = 11.84 RR18 pKa = 11.84 CGLSTGTTRR27 pKa = 11.84 PASSDD32 pKa = 3.25 NSPSARR38 pKa = 11.84 TPRR41 pKa = 11.84 SSSRR45 pKa = 11.84 RR46 pKa = 11.84 HH47 pKa = 4.94 SCPTRR52 pKa = 11.84 TLSRR56 pKa = 11.84 TRR58 pKa = 11.84 CTRR61 pKa = 11.84 RR62 pKa = 11.84 PRR64 pKa = 11.84 SGTAPTSPNSPSFPPGRR81 pKa = 11.84 RR82 pKa = 11.84 SGPSPARR89 pKa = 11.84 RR90 pKa = 11.84 LAPTTRR96 pKa = 11.84 TTSSRR101 pKa = 11.84 SSSNDD106 pKa = 2.67 TFGRR110 pKa = 11.84 PRR112 pKa = 11.84 PSTTSWSSSRR122 pKa = 11.84 RR123 pKa = 11.84 TRR125 pKa = 11.84 RR126 pKa = 11.84 PSRR129 pKa = 11.84 SRR131 pKa = 11.84 TPGPSGHH138 pKa = 6.92 AARR141 pKa = 11.84 RR142 pKa = 11.84 PFKK145 pKa = 10.46 RR146 pKa = 11.84 SRR148 pKa = 11.84 QCPSPRR154 pKa = 11.84 RR155 pKa = 11.84 KK156 pKa = 9.95 RR157 pKa = 11.84 SVTPDD162 pKa = 2.79 GSFGRR167 pKa = 11.84 TGG169 pKa = 2.9
Molecular weight: 18.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 10.745
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 12.998
Rodwell 12.047
Grimsley 12.559
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.769
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.184
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4650
9
4659
2070129
33
4108
444.3
49.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.471 ± 0.032
1.187 ± 0.011
5.936 ± 0.025
6.225 ± 0.035
3.726 ± 0.023
6.196 ± 0.033
2.373 ± 0.017
5.304 ± 0.023
5.351 ± 0.032
9.337 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.186 ± 0.014
3.778 ± 0.018
4.839 ± 0.026
4.231 ± 0.026
5.799 ± 0.026
8.382 ± 0.033
6.269 ± 0.028
6.384 ± 0.026
1.169 ± 0.012
2.858 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here