Trichinella spiralis (Trichina worm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Enoplea; Dorylaimia; Trichinellida; Trichinellidae; Trichinella

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18572 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5T3X3|E5T3X3_TRISP Uncharacterized protein OS=Trichinella spiralis OX=6334 GN=T01_1256 PE=4 SV=1
MM1 pKa = 7.84ASRR4 pKa = 11.84FLTLEE9 pKa = 3.6NAIDD13 pKa = 3.99YY14 pKa = 11.1LEE16 pKa = 4.3TLPPEE21 pKa = 4.31DD22 pKa = 3.59QINAEE27 pKa = 4.39VCQLPPCEE35 pKa = 5.15DD36 pKa = 3.49GNLTDD41 pKa = 4.24EE42 pKa = 4.3EE43 pKa = 5.08HH44 pKa = 8.13IEE46 pKa = 4.14EE47 pKa = 5.31DD48 pKa = 4.45DD49 pKa = 4.1LDD51 pKa = 4.02EE52 pKa = 4.99VMPLDD57 pKa = 3.36VCGEE61 pKa = 3.92VDD63 pKa = 3.61VTVDD67 pKa = 2.52SDD69 pKa = 4.46RR70 pKa = 11.84IVNEE74 pKa = 4.76DD75 pKa = 4.3DD76 pKa = 3.24VTTNKK81 pKa = 10.54GYY83 pKa = 10.94GYY85 pKa = 10.7NSKK88 pKa = 10.24CQNTQLPPYY97 pKa = 9.33EE98 pKa = 4.69AGSNNEE104 pKa = 3.34KK105 pKa = 9.65MPRR108 pKa = 11.84IFRR111 pKa = 11.84TCMM114 pKa = 3.15

Molecular weight: 12.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0V0YZ57|A0A0V0YZ57_TRISP Uncharacterized protein OS=Trichinella spiralis OX=6334 GN=T01_5345 PE=4 SV=1
RR1 pKa = 7.2KK2 pKa = 9.56EE3 pKa = 3.89KK4 pKa = 11.05VKK6 pKa = 10.05TRR8 pKa = 11.84TTRR11 pKa = 11.84SRR13 pKa = 11.84SRR15 pKa = 11.84TTRR18 pKa = 11.84SRR20 pKa = 11.84TRR22 pKa = 11.84TTRR25 pKa = 11.84SRR27 pKa = 11.84TRR29 pKa = 11.84TTRR32 pKa = 11.84SRR34 pKa = 11.84SRR36 pKa = 11.84TTRR39 pKa = 11.84SRR41 pKa = 11.84SRR43 pKa = 11.84TTRR46 pKa = 11.84SRR48 pKa = 11.84SRR50 pKa = 11.84TTRR53 pKa = 11.84SRR55 pKa = 3.15

Molecular weight: 6.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14258

4314

18572

8958104

30

15913

482.3

54.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.46 ± 0.016

2.653 ± 0.019

5.217 ± 0.012

6.301 ± 0.021

4.602 ± 0.013

4.986 ± 0.022

2.484 ± 0.008

5.57 ± 0.012

6.017 ± 0.021

9.638 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.407 ± 0.008

5.014 ± 0.013

4.346 ± 0.018

4.255 ± 0.015

5.632 ± 0.017

8.375 ± 0.021

5.294 ± 0.012

6.439 ± 0.014

1.21 ± 0.006

3.1 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski