Sewage-associated circular DNA virus-36

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UH70|A0A0B4UH70_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-36 OX=1592103 PE=3 SV=1
MM1 pKa = 7.48ARR3 pKa = 11.84TPRR6 pKa = 11.84TNAQRR11 pKa = 11.84YY12 pKa = 7.35FLTYY16 pKa = 10.01SQAHH20 pKa = 6.57ALDD23 pKa = 4.21IDD25 pKa = 3.91DD26 pKa = 5.26LANFLHH32 pKa = 6.82ALAPSWLEE40 pKa = 3.5IVQEE44 pKa = 3.95NHH46 pKa = 5.83QDD48 pKa = 3.62DD49 pKa = 5.41GIHH52 pKa = 5.18YY53 pKa = 10.11HH54 pKa = 6.3VVLCFDD60 pKa = 3.73EE61 pKa = 5.0RR62 pKa = 11.84FQRR65 pKa = 11.84ALDD68 pKa = 3.86VFDD71 pKa = 6.06LDD73 pKa = 3.61NHH75 pKa = 6.59HH76 pKa = 7.27PNIAIIKK83 pKa = 8.61NASGDD88 pKa = 3.86LNNRR92 pKa = 11.84RR93 pKa = 11.84HH94 pKa = 6.3YY95 pKa = 9.9IRR97 pKa = 11.84KK98 pKa = 8.85GADD101 pKa = 3.03RR102 pKa = 11.84PKK104 pKa = 10.6EE105 pKa = 4.17SEE107 pKa = 3.96HH108 pKa = 6.77TIADD112 pKa = 3.99HH113 pKa = 7.43KK114 pKa = 10.87KK115 pKa = 9.59GPCDD119 pKa = 3.85YY120 pKa = 10.33IIEE123 pKa = 4.99PDD125 pKa = 3.44TRR127 pKa = 11.84GEE129 pKa = 4.09VPPYY133 pKa = 8.56STSAGRR139 pKa = 11.84LNWGGILEE147 pKa = 4.37SAKK150 pKa = 10.69SEE152 pKa = 4.26EE153 pKa = 4.1EE154 pKa = 3.64FLILVRR160 pKa = 11.84VNQPTEE166 pKa = 3.46WVLRR170 pKa = 11.84NDD172 pKa = 5.18SIVKK176 pKa = 9.1YY177 pKa = 10.96AKK179 pKa = 8.52THH181 pKa = 5.15YY182 pKa = 9.85KK183 pKa = 10.01AARR186 pKa = 11.84EE187 pKa = 4.02PEE189 pKa = 4.12KK190 pKa = 10.9VYY192 pKa = 10.47PPEE195 pKa = 4.0SWVVPPALDD204 pKa = 3.29DD205 pKa = 3.54WVAEE209 pKa = 4.25VFSDD213 pKa = 3.46VSTRR217 pKa = 11.84GVLPRR222 pKa = 11.84PDD224 pKa = 3.45RR225 pKa = 11.84PKK227 pKa = 10.3TLLLVGPTRR236 pKa = 11.84LGKK239 pKa = 9.74SVWAKK244 pKa = 10.08SLGRR248 pKa = 11.84YY249 pKa = 9.67SYY251 pKa = 10.1MCGMWHH257 pKa = 6.6SDD259 pKa = 3.38EE260 pKa = 4.77FDD262 pKa = 3.24EE263 pKa = 4.94SAQYY267 pKa = 10.96LILDD271 pKa = 3.89DD272 pKa = 4.87FEE274 pKa = 6.74FDD276 pKa = 3.69FFHH279 pKa = 7.07GMRR282 pKa = 11.84KK283 pKa = 9.92AIWGAQEE290 pKa = 4.37VFTTTDD296 pKa = 2.3KK297 pKa = 10.13WRR299 pKa = 11.84KK300 pKa = 8.86GVARR304 pKa = 11.84WGKK307 pKa = 6.62PTIWICQEE315 pKa = 3.43EE316 pKa = 4.96HH317 pKa = 6.84NPFTAVNRR325 pKa = 11.84KK326 pKa = 7.7TGLAVMDD333 pKa = 3.47EE334 pKa = 4.61TEE336 pKa = 4.26RR337 pKa = 11.84DD338 pKa = 3.32WYY340 pKa = 10.35KK341 pKa = 10.01QNCVEE346 pKa = 4.07VHH348 pKa = 6.07VNTKK352 pKa = 10.13FIRR355 pKa = 11.84LRR357 pKa = 11.84TCSGRR362 pKa = 11.84SNRR365 pKa = 11.84RR366 pKa = 11.84DD367 pKa = 3.21III369 pKa = 3.88

Molecular weight: 42.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UH70|A0A0B4UH70_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-36 OX=1592103 PE=3 SV=1
MM1 pKa = 7.83AYY3 pKa = 9.78HH4 pKa = 6.95RR5 pKa = 11.84KK6 pKa = 6.8TRR8 pKa = 11.84RR9 pKa = 11.84GHH11 pKa = 5.3RR12 pKa = 11.84SKK14 pKa = 11.08RR15 pKa = 11.84KK16 pKa = 6.49TPKK19 pKa = 10.05RR20 pKa = 11.84LSQHH24 pKa = 4.48MVRR27 pKa = 11.84AIKK30 pKa = 10.31AISQGEE36 pKa = 4.52VEE38 pKa = 4.71TKK40 pKa = 10.21RR41 pKa = 11.84YY42 pKa = 7.96MVSTDD47 pKa = 2.43ISTALTAAGYY57 pKa = 10.51VSGNNAVIRR66 pKa = 11.84RR67 pKa = 11.84NFLSDD72 pKa = 4.17LPRR75 pKa = 11.84EE76 pKa = 4.33SNTAVNSDD84 pKa = 2.96ASFIGDD90 pKa = 3.55QINLRR95 pKa = 11.84GLRR98 pKa = 11.84WSFHH102 pKa = 5.01GSILPTVNPDD112 pKa = 2.28AMYY115 pKa = 10.83RR116 pKa = 11.84FTVYY120 pKa = 10.27RR121 pKa = 11.84DD122 pKa = 3.14SFYY125 pKa = 10.9FAGTNGPGPGDD136 pKa = 3.79RR137 pKa = 11.84IFEE140 pKa = 4.3QQQSTIPTWSMWNTQVTDD158 pKa = 3.09IVFQRR163 pKa = 11.84KK164 pKa = 7.09FRR166 pKa = 11.84MRR168 pKa = 11.84VSSIGSEE175 pKa = 4.05VLDD178 pKa = 3.49KK179 pKa = 11.1KK180 pKa = 10.85FYY182 pKa = 10.18IPLRR186 pKa = 11.84RR187 pKa = 11.84KK188 pKa = 8.26ITSNSDD194 pKa = 2.74EE195 pKa = 4.4SLTINTTMGAIKK207 pKa = 10.0GQQLYY212 pKa = 8.43WVLEE216 pKa = 4.38IYY218 pKa = 10.62AQGQTNLMTGIAGTIDD234 pKa = 3.17TVVYY238 pKa = 10.24FKK240 pKa = 11.04DD241 pKa = 3.22AA242 pKa = 3.57

Molecular weight: 27.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

611

242

369

305.5

35.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.71 ± 0.317

0.982 ± 0.608

6.547 ± 0.984

5.237 ± 1.453

4.255 ± 0.18

5.892 ± 0.702

3.11 ± 0.902

6.219 ± 0.755

5.237 ± 0.173

6.383 ± 0.626

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.73

4.583 ± 0.233

4.583 ± 0.791

3.601 ± 0.841

8.183 ± 0.306

6.547 ± 1.32

6.874 ± 1.373

6.547 ± 0.216

2.619 ± 0.598

3.764 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski