Pseudoalteromonas phage PHS3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0GVM1|A0A1J0GVM1_9CAUD Gene transfer agent portal protein OS=Pseudoalteromonas phage PHS3 OX=1913111 PE=4 SV=1
MM1 pKa = 7.34TPAEE5 pKa = 4.51YY6 pKa = 10.52LVLWDD11 pKa = 4.04YY12 pKa = 11.58KK13 pKa = 10.75YY14 pKa = 10.71ATAPEE19 pKa = 4.12YY20 pKa = 11.08AEE22 pKa = 3.76QRR24 pKa = 11.84KK25 pKa = 9.47ASEE28 pKa = 4.37LDD30 pKa = 3.12QALIDD35 pKa = 3.89NWDD38 pKa = 3.67LLLL41 pKa = 5.35

Molecular weight: 4.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0GVL4|A0A1J0GVL4_9CAUD Putative head-tail adaptor protein OS=Pseudoalteromonas phage PHS3 OX=1913111 PE=4 SV=1
MM1 pKa = 7.69NDD3 pKa = 3.35LSGMTDD9 pKa = 3.54LSFTQTKK16 pKa = 9.99RR17 pKa = 11.84AMTSTHH23 pKa = 6.87LSRR26 pKa = 11.84VSHH29 pKa = 6.13QYY31 pKa = 10.12RR32 pKa = 11.84PVWAIAATNNAKK44 pKa = 10.27PKK46 pKa = 10.02VVIDD50 pKa = 3.65RR51 pKa = 11.84VRR53 pKa = 11.84DD54 pKa = 3.88GKK56 pKa = 10.8RR57 pKa = 11.84SNPTVFTSLL66 pKa = 3.45

Molecular weight: 7.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

9837

33

710

169.6

18.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.631 ± 0.514

1.078 ± 0.181

6.242 ± 0.33

7.187 ± 0.362

3.629 ± 0.19

6.465 ± 0.523

1.393 ± 0.153

6.333 ± 0.288

7.492 ± 0.447

8.397 ± 0.353

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.775 ± 0.205

5.469 ± 0.213

3.253 ± 0.259

4.127 ± 0.348

4.381 ± 0.262

6.72 ± 0.336

5.601 ± 0.368

6.069 ± 0.213

1.2 ± 0.129

3.558 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski