Melon yellowing-associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W7HH44|A0A1W7HH44_9VIRU 7 kDa protein OS=Melon yellowing-associated virus OX=255255 GN=TGB3 PE=3 SV=1
MM1 pKa = 7.21WCEE4 pKa = 3.91SLIGLIAASLTFLILIYY21 pKa = 9.86FSRR24 pKa = 11.84EE25 pKa = 3.45NEE27 pKa = 4.12TCVLIVSKK35 pKa = 10.97SSLVVKK41 pKa = 10.65GCGNNVPDD49 pKa = 4.94ISNLDD54 pKa = 3.53LSFLQGVSCDD64 pKa = 3.7GLL66 pKa = 3.72

Molecular weight: 7.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W7HH39|A0A1W7HH39_9VIRU Capsid protein OS=Melon yellowing-associated virus OX=255255 GN=CP PE=3 SV=1
MM1 pKa = 8.0DD2 pKa = 5.57LVLKK6 pKa = 10.6QDD8 pKa = 3.93VGWLTVLLARR18 pKa = 11.84RR19 pKa = 11.84MGLDD23 pKa = 3.37YY24 pKa = 10.98DD25 pKa = 4.09ISRR28 pKa = 11.84SIAKK32 pKa = 10.02YY33 pKa = 9.13VVNYY37 pKa = 10.55NNGIRR42 pKa = 11.84TYY44 pKa = 9.68EE45 pKa = 4.12SYY47 pKa = 11.97VNGSSKK53 pKa = 10.32SAKK56 pKa = 9.19KK57 pKa = 10.03RR58 pKa = 11.84RR59 pKa = 11.84AKK61 pKa = 10.36RR62 pKa = 11.84FQVCVKK68 pKa = 9.89CARR71 pKa = 11.84PHH73 pKa = 5.28CTNGINCVPNTSSQINVSNLIEE95 pKa = 4.24MGVTRR100 pKa = 11.84YY101 pKa = 8.13LTEE104 pKa = 3.73STPRR108 pKa = 11.84KK109 pKa = 7.58GTHH112 pKa = 5.95VYY114 pKa = 10.9DD115 pKa = 3.83HH116 pKa = 6.73VKK118 pKa = 10.75SEE120 pKa = 4.38LGLIKK125 pKa = 10.84YY126 pKa = 9.98NSLKK130 pKa = 10.69VKK132 pKa = 10.46PKK134 pKa = 10.46

Molecular weight: 15.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2989

66

2087

498.2

56.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.018 ± 0.542

2.509 ± 0.482

5.587 ± 0.498

6.858 ± 1.382

5.989 ± 1.086

6.056 ± 0.366

1.974 ± 0.145

6.39 ± 0.486

7.494 ± 0.674

9.167 ± 0.646

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.041 ± 0.284

5.219 ± 0.673

3.446 ± 0.448

2.777 ± 0.349

5.286 ± 0.365

8.13 ± 1.569

5.286 ± 0.62

6.557 ± 0.677

0.87 ± 0.075

3.312 ± 0.68
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski