Mycoplasma falconis
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 563 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A501XBZ3|A0A501XBZ3_9MOLU 30S ribosomal protein S14 type Z OS=Mycoplasma falconis OX=92403 GN=rpsZ PE=3 SV=1
MM1 pKa = 7.46 LSSVHH6 pKa = 6.69 CFGCSEE12 pKa = 4.24 EE13 pKa = 4.51 LFSTGLAGSLLVSSVSVALFSPLVFEE39 pKa = 4.63 VWLSSVEE46 pKa = 4.08 VLSLSSGFASVFGWSSLSFF65 pKa = 3.9
Molecular weight: 6.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.109
IPC2_protein 4.431
IPC_protein 3.961
Toseland 3.897
ProMoST 4.113
Dawson 3.91
Bjellqvist 4.088
Wikipedia 3.808
Rodwell 3.846
Grimsley 3.846
Solomon 3.834
Lehninger 3.795
Nozaki 4.101
DTASelect 3.986
Thurlkill 3.948
EMBOSS 3.821
Sillero 4.088
Patrickios 0.299
IPC_peptide 3.859
IPC2_peptide 4.062
IPC2.peptide.svr19 4.008
Protein with the highest isoelectric point:
>tr|A0A501X9V8|A0A501X9V8_9MOLU Uncharacterized protein OS=Mycoplasma falconis OX=92403 GN=FJO69_02115 PE=4 SV=1
MM1 pKa = 7.73 ADD3 pKa = 3.69 KK4 pKa = 10.46 IRR6 pKa = 11.84 YY7 pKa = 8.14 YY8 pKa = 11.18 GLGRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 10.01 SSVARR19 pKa = 11.84 VYY21 pKa = 10.83 LLPGKK26 pKa = 10.55 GSFIINGKK34 pKa = 6.89 EE35 pKa = 3.59 AKK37 pKa = 10.07 SYY39 pKa = 10.96 LNSDD43 pKa = 3.66 ILIKK47 pKa = 10.79 DD48 pKa = 3.48 ALSPFALTEE57 pKa = 4.02 TEE59 pKa = 3.93 KK60 pKa = 11.0 QFDD63 pKa = 3.33 VFANVNGGGLSGQAGAIRR81 pKa = 11.84 LGIARR86 pKa = 11.84 ALLEE90 pKa = 4.27 ASNNEE95 pKa = 3.75 YY96 pKa = 10.71 RR97 pKa = 11.84 NSLKK101 pKa = 10.79 DD102 pKa = 3.29 AGFLTRR108 pKa = 11.84 DD109 pKa = 3.24 ARR111 pKa = 11.84 VKK113 pKa = 9.75 EE114 pKa = 4.1 RR115 pKa = 11.84 KK116 pKa = 9.77 KK117 pKa = 10.69 FGLRR121 pKa = 11.84 KK122 pKa = 9.46 ARR124 pKa = 11.84 RR125 pKa = 11.84 ARR127 pKa = 11.84 QFSKK131 pKa = 10.87 RR132 pKa = 3.51
Molecular weight: 14.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.304
IPC2_protein 9.853
IPC_protein 10.555
Toseland 10.847
ProMoST 10.643
Dawson 10.935
Bjellqvist 10.599
Wikipedia 11.111
Rodwell 11.272
Grimsley 10.965
Solomon 11.038
Lehninger 11.008
Nozaki 10.818
DTASelect 10.599
Thurlkill 10.833
EMBOSS 11.242
Sillero 10.847
Patrickios 10.979
IPC_peptide 11.038
IPC2_peptide 9.282
IPC2.peptide.svr19 8.624
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
563
0
563
204316
37
3375
362.9
41.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.042 ± 0.128
0.517 ± 0.025
5.74 ± 0.085
7.067 ± 0.093
4.905 ± 0.09
4.768 ± 0.102
1.381 ± 0.043
9.139 ± 0.119
10.091 ± 0.116
9.567 ± 0.099
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.88 ± 0.039
7.961 ± 0.171
2.869 ± 0.063
3.419 ± 0.052
2.869 ± 0.081
5.88 ± 0.067
4.87 ± 0.057
5.7 ± 0.089
0.948 ± 0.036
4.388 ± 0.07
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here