Capybara microvirus Cap3_SP_316 
Average proteome isoelectric point is 5.71 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A4V1FVR9|A0A4V1FVR9_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_316 OX=2585426 PE=3 SV=1 
MM1 pKa = 6.85  TTDD4 pKa = 3.26  KK5 pKa = 11.08  LFYY8 pKa = 10.35  IYY10 pKa = 9.74  DD11 pKa = 3.9  TVSDD15 pKa = 4.32  LNLGYY20 pKa = 10.42  RR21 pKa = 11.84  FYY23 pKa = 10.01  PTIGALIRR31 pKa = 11.84  SEE33 pKa = 4.41  APTISKK39 pKa = 9.19  QFPNFEE45 pKa = 4.28  TEE47 pKa = 4.03  LKK49 pKa = 10.15  IIEE52 pKa = 4.47  IGSFVSDD59 pKa = 2.93  SGYY62 pKa = 10.83  DD63 pKa = 3.06  ISSFNPLSVLDD74 pKa = 3.97  IPLIHH79 pKa = 6.62  SWDD82 pKa = 3.75  EE83 pKa = 3.83  YY84 pKa = 11.16  SFQSSEE90 pKa = 4.18  TKK92 pKa = 10.18  FNKK95 pKa = 9.97   
 Molecular weight: 10.91 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  4.379 
IPC2_protein 4.482 
IPC_protein 4.342 
Toseland    4.164 
ProMoST     4.482 
Dawson      4.304 
Bjellqvist  4.457 
Wikipedia   4.215 
Rodwell     4.177 
Grimsley    4.075 
Solomon     4.304 
Lehninger   4.253 
Nozaki      4.431 
DTASelect   4.609 
Thurlkill   4.202 
EMBOSS      4.228 
Sillero     4.457 
Patrickios  3.427 
IPC_peptide 4.304 
IPC2_peptide  4.444 
IPC2.peptide.svr19  4.393 
 Protein with the highest isoelectric point: 
>tr|A0A4P8W4Q1|A0A4P8W4Q1_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_316 OX=2585426 PE=4 SV=1 
MM1 pKa = 7.63  ICRR4 pKa = 11.84  NPIDD8 pKa = 3.75  SSNFKK13 pKa = 10.83  YY14 pKa = 10.21  PIYY17 pKa = 10.11  DD18 pKa = 3.27  ALGRR22 pKa = 11.84  TVKK25 pKa = 10.33  VPCNTCLACRR35 pKa = 11.84  ANKK38 pKa = 9.3  ILNYY42 pKa = 8.69  SARR45 pKa = 11.84  ISYY48 pKa = 9.83  EE49 pKa = 3.61  RR50 pKa = 11.84  INNPEE55 pKa = 3.9  CSFVTFTYY63 pKa = 10.73  DD64 pKa = 4.43  KK65 pKa = 11.46  NHH67 pKa = 6.37  LKK69 pKa = 10.96  YY70 pKa = 10.9  NFGFYY75 pKa = 10.76  EE76 pKa = 4.19  PTLKK80 pKa = 10.69  RR81 pKa = 11.84  SDD83 pKa = 3.1  LHH85 pKa = 8.49  KK86 pKa = 11.13  YY87 pKa = 8.29  IDD89 pKa = 3.89  KK90 pKa = 10.54  VRR92 pKa = 11.84  HH93 pKa = 5.86  LLPKK97 pKa = 10.14  DD98 pKa = 3.44  VKK100 pKa = 10.45  FKK102 pKa = 11.14  YY103 pKa = 9.49  FASGQYY109 pKa = 10.09  GDD111 pKa = 4.05  RR112 pKa = 11.84  FNRR115 pKa = 11.84  PHH117 pKa = 5.7  YY118 pKa = 9.7  HH119 pKa = 5.55  VLFIGLDD126 pKa = 3.43  FVKK129 pKa = 10.51  YY130 pKa = 10.26  YY131 pKa = 11.18  KK132 pKa = 10.42  FFKK135 pKa = 10.62  DD136 pKa = 3.07  SWNLGSVDD144 pKa = 4.11  VQPMLSGSVQYY155 pKa = 10.63  VCSYY159 pKa = 10.59  LHH161 pKa = 5.87  HH162 pKa = 6.61  QKK164 pKa = 10.66  FGSDD168 pKa = 3.3  SLDD171 pKa = 3.09  SQFFDD176 pKa = 3.96  NGLDD180 pKa = 3.62  EE181 pKa = 5.46  PFLSMSPGIGSDD193 pKa = 4.07  FYY195 pKa = 10.97  LAHH198 pKa = 7.02  IFEE201 pKa = 5.45  LLNGLPVFYY210 pKa = 10.16  GSSSVQVPSYY220 pKa = 10.68  YY221 pKa = 9.69  RR222 pKa = 11.84  RR223 pKa = 11.84  KK224 pKa = 9.62  YY225 pKa = 10.67  LKK227 pKa = 10.5  LSQFSLDD234 pKa = 3.88  NIRR237 pKa = 11.84  DD238 pKa = 3.84  SLVLNRR244 pKa = 11.84  KK245 pKa = 6.98  YY246 pKa = 10.43  HH247 pKa = 5.17  YY248 pKa = 11.14  NSMLEE253 pKa = 3.63  AGYY256 pKa = 9.13  TDD258 pKa = 3.86  FGKK261 pKa = 10.09  FVKK264 pKa = 9.66  MKK266 pKa = 10.64  SEE268 pKa = 3.89  QQDD271 pKa = 3.52  FQLINSLRR279 pKa = 11.84  SRR281 pKa = 11.84  GCPISQSYY289 pKa = 10.43  LFGNTVYY296 pKa = 10.98  SFDD299 pKa = 3.55  KK300 pKa = 11.37  GFINSLANSALKK312 pKa = 10.58  AA313 pKa = 3.66   
 Molecular weight: 36.36 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  8.575 
IPC2_protein 8.551 
IPC_protein 8.448 
Toseland    8.77 
ProMoST     8.843 
Dawson      9.194 
Bjellqvist  9.151 
Wikipedia   9.385 
Rodwell     9.282 
Grimsley    9.224 
Solomon     9.253 
Lehninger   9.209 
Nozaki      9.151 
DTASelect   9.033 
Thurlkill   9.077 
EMBOSS      9.297 
Sillero     9.268 
Patrickios  3.719 
IPC_peptide 9.238 
IPC2_peptide  7.966 
IPC2.peptide.svr19  7.788 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        7 
 
        
        0
 
        
        7 
         
        1846
 
        77
 
        650
 
        263.7
 
        29.86
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        3.629 ± 0.463
1.138 ± 0.385
 
        7.367 ± 0.616
3.575 ± 0.433
 
        7.259 ± 0.542
5.525 ± 0.53
 
        1.463 ± 0.333
6.013 ± 0.613
       
        5.742 ± 0.7
9.751 ± 0.4
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        1.138 ± 0.143
7.638 ± 0.388
 
        4.28 ± 0.882
3.359 ± 0.375
 
        4.009 ± 0.21
13.868 ± 0.935
 
        3.738 ± 0.647
4.388 ± 0.529
       
        0.704 ± 0.162
5.417 ± 0.679
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here