Capybara microvirus Cap3_SP_316
Average proteome isoelectric point is 5.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVR9|A0A4V1FVR9_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_316 OX=2585426 PE=3 SV=1
MM1 pKa = 6.85 TTDD4 pKa = 3.26 KK5 pKa = 11.08 LFYY8 pKa = 10.35 IYY10 pKa = 9.74 DD11 pKa = 3.9 TVSDD15 pKa = 4.32 LNLGYY20 pKa = 10.42 RR21 pKa = 11.84 FYY23 pKa = 10.01 PTIGALIRR31 pKa = 11.84 SEE33 pKa = 4.41 APTISKK39 pKa = 9.19 QFPNFEE45 pKa = 4.28 TEE47 pKa = 4.03 LKK49 pKa = 10.15 IIEE52 pKa = 4.47 IGSFVSDD59 pKa = 2.93 SGYY62 pKa = 10.83 DD63 pKa = 3.06 ISSFNPLSVLDD74 pKa = 3.97 IPLIHH79 pKa = 6.62 SWDD82 pKa = 3.75 EE83 pKa = 3.83 YY84 pKa = 11.16 SFQSSEE90 pKa = 4.18 TKK92 pKa = 10.18 FNKK95 pKa = 9.97
Molecular weight: 10.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.379
IPC2_protein 4.482
IPC_protein 4.342
Toseland 4.164
ProMoST 4.482
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.215
Rodwell 4.177
Grimsley 4.075
Solomon 4.304
Lehninger 4.253
Nozaki 4.431
DTASelect 4.609
Thurlkill 4.202
EMBOSS 4.228
Sillero 4.457
Patrickios 3.427
IPC_peptide 4.304
IPC2_peptide 4.444
IPC2.peptide.svr19 4.393
Protein with the highest isoelectric point:
>tr|A0A4P8W4Q1|A0A4P8W4Q1_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_316 OX=2585426 PE=4 SV=1
MM1 pKa = 7.63 ICRR4 pKa = 11.84 NPIDD8 pKa = 3.75 SSNFKK13 pKa = 10.83 YY14 pKa = 10.21 PIYY17 pKa = 10.11 DD18 pKa = 3.27 ALGRR22 pKa = 11.84 TVKK25 pKa = 10.33 VPCNTCLACRR35 pKa = 11.84 ANKK38 pKa = 9.3 ILNYY42 pKa = 8.69 SARR45 pKa = 11.84 ISYY48 pKa = 9.83 EE49 pKa = 3.61 RR50 pKa = 11.84 INNPEE55 pKa = 3.9 CSFVTFTYY63 pKa = 10.73 DD64 pKa = 4.43 KK65 pKa = 11.46 NHH67 pKa = 6.37 LKK69 pKa = 10.96 YY70 pKa = 10.9 NFGFYY75 pKa = 10.76 EE76 pKa = 4.19 PTLKK80 pKa = 10.69 RR81 pKa = 11.84 SDD83 pKa = 3.1 LHH85 pKa = 8.49 KK86 pKa = 11.13 YY87 pKa = 8.29 IDD89 pKa = 3.89 KK90 pKa = 10.54 VRR92 pKa = 11.84 HH93 pKa = 5.86 LLPKK97 pKa = 10.14 DD98 pKa = 3.44 VKK100 pKa = 10.45 FKK102 pKa = 11.14 YY103 pKa = 9.49 FASGQYY109 pKa = 10.09 GDD111 pKa = 4.05 RR112 pKa = 11.84 FNRR115 pKa = 11.84 PHH117 pKa = 5.7 YY118 pKa = 9.7 HH119 pKa = 5.55 VLFIGLDD126 pKa = 3.43 FVKK129 pKa = 10.51 YY130 pKa = 10.26 YY131 pKa = 11.18 KK132 pKa = 10.42 FFKK135 pKa = 10.62 DD136 pKa = 3.07 SWNLGSVDD144 pKa = 4.11 VQPMLSGSVQYY155 pKa = 10.63 VCSYY159 pKa = 10.59 LHH161 pKa = 5.87 HH162 pKa = 6.61 QKK164 pKa = 10.66 FGSDD168 pKa = 3.3 SLDD171 pKa = 3.09 SQFFDD176 pKa = 3.96 NGLDD180 pKa = 3.62 EE181 pKa = 5.46 PFLSMSPGIGSDD193 pKa = 4.07 FYY195 pKa = 10.97 LAHH198 pKa = 7.02 IFEE201 pKa = 5.45 LLNGLPVFYY210 pKa = 10.16 GSSSVQVPSYY220 pKa = 10.68 YY221 pKa = 9.69 RR222 pKa = 11.84 RR223 pKa = 11.84 KK224 pKa = 9.62 YY225 pKa = 10.67 LKK227 pKa = 10.5 LSQFSLDD234 pKa = 3.88 NIRR237 pKa = 11.84 DD238 pKa = 3.84 SLVLNRR244 pKa = 11.84 KK245 pKa = 6.98 YY246 pKa = 10.43 HH247 pKa = 5.17 YY248 pKa = 11.14 NSMLEE253 pKa = 3.63 AGYY256 pKa = 9.13 TDD258 pKa = 3.86 FGKK261 pKa = 10.09 FVKK264 pKa = 9.66 MKK266 pKa = 10.64 SEE268 pKa = 3.89 QQDD271 pKa = 3.52 FQLINSLRR279 pKa = 11.84 SRR281 pKa = 11.84 GCPISQSYY289 pKa = 10.43 LFGNTVYY296 pKa = 10.98 SFDD299 pKa = 3.55 KK300 pKa = 11.37 GFINSLANSALKK312 pKa = 10.58 AA313 pKa = 3.66
Molecular weight: 36.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.575
IPC2_protein 8.551
IPC_protein 8.448
Toseland 8.77
ProMoST 8.843
Dawson 9.194
Bjellqvist 9.151
Wikipedia 9.385
Rodwell 9.282
Grimsley 9.224
Solomon 9.253
Lehninger 9.209
Nozaki 9.151
DTASelect 9.033
Thurlkill 9.077
EMBOSS 9.297
Sillero 9.268
Patrickios 3.719
IPC_peptide 9.238
IPC2_peptide 7.966
IPC2.peptide.svr19 7.788
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1846
77
650
263.7
29.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.629 ± 0.463
1.138 ± 0.385
7.367 ± 0.616
3.575 ± 0.433
7.259 ± 0.542
5.525 ± 0.53
1.463 ± 0.333
6.013 ± 0.613
5.742 ± 0.7
9.751 ± 0.4
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.138 ± 0.143
7.638 ± 0.388
4.28 ± 0.882
3.359 ± 0.375
4.009 ± 0.21
13.868 ± 0.935
3.738 ± 0.647
4.388 ± 0.529
0.704 ± 0.162
5.417 ± 0.679
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here