Candida maltosa (strain Xu316) (Yeast)
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5976 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M3HFY7|M3HFY7_CANMX Alpha-1 2-mannosyltransferase putative OS=Candida maltosa (strain Xu316) OX=1245528 GN=G210_3625 PE=3 SV=1
MM1 pKa = 7.67 IFLLLLSLIVATVVEE16 pKa = 4.88 CSLKK20 pKa = 10.12 IDD22 pKa = 4.1 FEE24 pKa = 4.46 VHH26 pKa = 5.6 KK27 pKa = 11.05 ASFNPHH33 pKa = 4.6 LMKK36 pKa = 10.56 RR37 pKa = 11.84 SSPVLSIINNKK48 pKa = 8.93 SLYY51 pKa = 8.29 ITSLAIGSNKK61 pKa = 10.18 DD62 pKa = 3.78 PISVSIDD69 pKa = 3.06 TGSADD74 pKa = 3.66 LWVMGSDD81 pKa = 3.54 VTCFDD86 pKa = 3.4 TSEE89 pKa = 4.25 LHH91 pKa = 6.73 VDD93 pKa = 4.44 GAPSLPEE100 pKa = 3.88 IFDD103 pKa = 5.56 DD104 pKa = 4.31 INPDD108 pKa = 3.53 YY109 pKa = 11.38 SCTANGTFDD118 pKa = 3.73 TANSTTFKK126 pKa = 10.4 STDD129 pKa = 3.25 DD130 pKa = 3.69 NFVIGYY136 pKa = 7.95 TDD138 pKa = 3.76 GSAAIGKK145 pKa = 8.59 WGIDD149 pKa = 3.01 SVQFGNSTINEE160 pKa = 4.12 LRR162 pKa = 11.84 LGIASQSSVSDD173 pKa = 4.41 GILGIGIPDD182 pKa = 4.38 GYY184 pKa = 11.16 DD185 pKa = 3.0 NFPIQLKK192 pKa = 7.92 NQKK195 pKa = 10.38 LIDD198 pKa = 3.56 KK199 pKa = 9.23 VAYY202 pKa = 9.47 SVYY205 pKa = 10.45 LNSSDD210 pKa = 4.96 AIGGTVLFGAIDD222 pKa = 3.4 HH223 pKa = 6.69 AKK225 pKa = 10.67 YY226 pKa = 10.27 EE227 pKa = 4.44 GALTTVPLTSDD238 pKa = 3.18 TLLSVNVTYY247 pKa = 11.01 EE248 pKa = 3.87 NVNYY252 pKa = 10.6 SVILDD257 pKa = 3.67 TGSTFSIFPDD267 pKa = 2.89 TWINDD272 pKa = 3.17 FGTLLNGTYY281 pKa = 10.49 DD282 pKa = 3.82 DD283 pKa = 4.01 NEE285 pKa = 3.79 EE286 pKa = 4.35 VYY288 pKa = 10.67 RR289 pKa = 11.84 IDD291 pKa = 4.36 CDD293 pKa = 3.7 SRR295 pKa = 11.84 DD296 pKa = 3.61 DD297 pKa = 3.82 FFEE300 pKa = 4.14 FEE302 pKa = 4.22 IGNASFAIPVEE313 pKa = 4.08 DD314 pKa = 5.48 FIVEE318 pKa = 4.02 NDD320 pKa = 3.86 DD321 pKa = 3.44 VCYY324 pKa = 9.92 LAIMGNSVIGGDD336 pKa = 3.96 GILFGGDD343 pKa = 2.99 ILRR346 pKa = 11.84 SIYY349 pKa = 10.45 LVYY352 pKa = 10.7 DD353 pKa = 3.67 LEE355 pKa = 5.2 DD356 pKa = 3.39 RR357 pKa = 11.84 TISVAPVRR365 pKa = 11.84 YY366 pKa = 8.27 TDD368 pKa = 4.72 DD369 pKa = 3.52 EE370 pKa = 5.19 DD371 pKa = 4.76 IEE373 pKa = 4.37 EE374 pKa = 4.98 LGNQTVAVDD383 pKa = 3.55 NSTVSTSQTSVLPTSQTSALSSTSSVNQARR413 pKa = 11.84 VIGSFTVSTLMGFILTFII431 pKa = 4.55
Molecular weight: 46.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.77
IPC_protein 3.821
Toseland 3.579
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.63
Grimsley 3.49
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.202
Thurlkill 3.63
EMBOSS 3.77
Sillero 3.935
Patrickios 1.901
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|M3JVG2|M3JVG2_CANMX Elongin-A OS=Candida maltosa (strain Xu316) OX=1245528 GN=G210_2836 PE=4 SV=1
SS1 pKa = 5.96 TRR3 pKa = 11.84 ATSCTTTTNSSSTVASSGSANTSTRR28 pKa = 11.84 VTRR31 pKa = 11.84 SRR33 pKa = 11.84 TTTRR37 pKa = 11.84 STTARR42 pKa = 11.84 KK43 pKa = 8.19 STTVSTILPPVSSGPLKK60 pKa = 10.93 NITNTVSARR69 pKa = 11.84 SRR71 pKa = 11.84 RR72 pKa = 11.84 VKK74 pKa = 9.58 TASKK78 pKa = 10.41 KK79 pKa = 10.15
Molecular weight: 8.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.377
IPC2_protein 10.804
IPC_protein 12.384
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.281
Grimsley 12.588
Solomon 13.042
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.018
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5976
0
5976
2856021
10
4888
477.9
53.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.178 ± 0.031
1.057 ± 0.012
6.147 ± 0.027
6.722 ± 0.035
4.504 ± 0.022
5.125 ± 0.03
2.089 ± 0.014
6.886 ± 0.025
7.27 ± 0.033
9.182 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.823 ± 0.011
6.178 ± 0.029
4.697 ± 0.03
4.258 ± 0.032
3.792 ± 0.02
8.701 ± 0.042
6.082 ± 0.032
5.772 ± 0.025
0.99 ± 0.011
3.549 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here