Faeces associated gemycircularvirus 3
Average proteome isoelectric point is 7.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T1YTC0|T1YTC0_9VIRU Capsid protein OS=Faeces associated gemycircularvirus 3 OX=1391032 PE=4 SV=1
MM1 pKa = 7.55 PPKK4 pKa = 10.38 AKK6 pKa = 9.95 PNPYY10 pKa = 9.72 FADD13 pKa = 3.11 GRR15 pKa = 11.84 YY16 pKa = 9.19 FLLTYY21 pKa = 8.77 AQCGTLDD28 pKa = 2.95 AWTVNDD34 pKa = 3.97 HH35 pKa = 6.64 LAFLGAEE42 pKa = 4.25 CIIGRR47 pKa = 11.84 EE48 pKa = 4.02 LHH50 pKa = 6.83 ADD52 pKa = 3.72 GGTHH56 pKa = 6.78 LHH58 pKa = 6.66 AFCDD62 pKa = 5.08 FSRR65 pKa = 11.84 RR66 pKa = 11.84 FRR68 pKa = 11.84 SRR70 pKa = 11.84 RR71 pKa = 11.84 SDD73 pKa = 3.3 VFDD76 pKa = 3.2 VGGRR80 pKa = 11.84 HH81 pKa = 6.27 PNLVPSYY88 pKa = 8.82 GKK90 pKa = 9.76 PEE92 pKa = 3.92 GGYY95 pKa = 10.65 DD96 pKa = 3.55 YY97 pKa = 10.74 AIKK100 pKa = 10.94 DD101 pKa = 3.59 GDD103 pKa = 4.03 VVAGGLSRR111 pKa = 11.84 PSGRR115 pKa = 11.84 GVYY118 pKa = 10.06 EE119 pKa = 4.89 DD120 pKa = 3.54 VSSWSIIVGQEE131 pKa = 3.87 SEE133 pKa = 4.58 GEE135 pKa = 3.92 FWKK138 pKa = 10.87 CVARR142 pKa = 11.84 LDD144 pKa = 4.55 PRR146 pKa = 11.84 ALCTNYY152 pKa = 10.56 NSLRR156 pKa = 11.84 AYY158 pKa = 10.43 ANWRR162 pKa = 11.84 YY163 pKa = 9.76 RR164 pKa = 11.84 PAPVPYY170 pKa = 8.89 EE171 pKa = 4.04 HH172 pKa = 7.45 PAGIEE177 pKa = 4.08 FEE179 pKa = 4.46 LGMVPEE185 pKa = 4.27 LAVWRR190 pKa = 11.84 EE191 pKa = 3.56 IALGADD197 pKa = 3.77 RR198 pKa = 11.84 EE199 pKa = 4.16 RR200 pKa = 11.84 GILVIFGDD208 pKa = 3.67 TRR210 pKa = 11.84 LGKK213 pKa = 7.65 TLWARR218 pKa = 11.84 SIGPHH223 pKa = 6.89 IYY225 pKa = 10.2 TIGQMSGEE233 pKa = 4.38 VILRR237 pKa = 11.84 DD238 pKa = 4.51 GPDD241 pKa = 2.84 ADD243 pKa = 3.79 YY244 pKa = 11.53 AVFDD248 pKa = 4.39 DD249 pKa = 4.11 MRR251 pKa = 11.84 GGLEE255 pKa = 4.55 FFHH258 pKa = 6.92 GWKK261 pKa = 9.71 EE262 pKa = 3.86 WFGCQSVVTVKK273 pKa = 10.58 KK274 pKa = 10.58 LYY276 pKa = 9.92 RR277 pKa = 11.84 DD278 pKa = 3.77 PVQMPWGKK286 pKa = 9.33 PVIWLSNRR294 pKa = 11.84 DD295 pKa = 3.57 PRR297 pKa = 11.84 DD298 pKa = 3.33 EE299 pKa = 4.41 LRR301 pKa = 11.84 DD302 pKa = 3.78 SITNHH307 pKa = 4.96 TSMGKK312 pKa = 8.57 QASIEE317 pKa = 4.03 GDD319 pKa = 3.59 IKK321 pKa = 10.77 WLDD324 pKa = 3.56 GNCIFVEE331 pKa = 4.06 LDD333 pKa = 3.15 HH334 pKa = 7.64 AIFRR338 pKa = 11.84 ANTEE342 pKa = 3.84
Molecular weight: 38.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.581
IPC2_protein 5.563
IPC_protein 5.588
Toseland 5.931
ProMoST 5.792
Dawson 5.804
Bjellqvist 5.842
Wikipedia 5.804
Rodwell 5.779
Grimsley 6.059
Solomon 5.804
Lehninger 5.792
Nozaki 6.059
DTASelect 6.249
Thurlkill 6.224
EMBOSS 6.186
Sillero 6.148
Patrickios 4.037
IPC_peptide 5.817
IPC2_peptide 6.16
IPC2.peptide.svr19 6.124
Protein with the highest isoelectric point:
>tr|T1YTC0|T1YTC0_9VIRU Capsid protein OS=Faeces associated gemycircularvirus 3 OX=1391032 PE=4 SV=1
MM1 pKa = 7.78 AYY3 pKa = 9.79 RR4 pKa = 11.84 RR5 pKa = 11.84 KK6 pKa = 10.16 SYY8 pKa = 10.45 SSRR11 pKa = 11.84 VKK13 pKa = 10.44 RR14 pKa = 11.84 RR15 pKa = 11.84 TGRR18 pKa = 11.84 TARR21 pKa = 11.84 RR22 pKa = 11.84 GGRR25 pKa = 11.84 RR26 pKa = 11.84 PASQQKK32 pKa = 9.42 RR33 pKa = 11.84 RR34 pKa = 11.84 TYY36 pKa = 9.73 RR37 pKa = 11.84 KK38 pKa = 9.3 KK39 pKa = 10.36 GAMSKK44 pKa = 10.36 KK45 pKa = 10.15 RR46 pKa = 11.84 ILNLTSRR53 pKa = 11.84 KK54 pKa = 9.72 KK55 pKa = 10.4 RR56 pKa = 11.84 DD57 pKa = 3.46 TMLTWTNTTTSGGQVSPRR75 pKa = 11.84 PDD77 pKa = 3.01 AAYY80 pKa = 10.91 VKK82 pKa = 10.26 GDD84 pKa = 3.51 TGGSFLWVPTARR96 pKa = 11.84 DD97 pKa = 3.49 LVVGSNLATIALEE110 pKa = 4.1 SARR113 pKa = 11.84 TSTTCYY119 pKa = 9.77 MRR121 pKa = 11.84 GLSEE125 pKa = 4.49 KK126 pKa = 10.44 LRR128 pKa = 11.84 LSTSSGVPWFHH139 pKa = 7.15 RR140 pKa = 11.84 RR141 pKa = 11.84 ICFTFKK147 pKa = 10.42 GAQPFQSNFTGDD159 pKa = 3.08 TGSNQLYY166 pKa = 10.44 LEE168 pKa = 4.35 NSNGLTRR175 pKa = 11.84 LMFNQAVTIPPNYY188 pKa = 9.18 QNQINSVIFKK198 pKa = 10.02 GAQGVDD204 pKa = 2.93 WSDD207 pKa = 3.79 VIIAPIDD214 pKa = 3.39 TRR216 pKa = 11.84 RR217 pKa = 11.84 ISLKK221 pKa = 10.16 YY222 pKa = 10.23 DD223 pKa = 3.26 RR224 pKa = 11.84 TWTYY228 pKa = 11.2 RR229 pKa = 11.84 SGNANGTVMEE239 pKa = 4.38 KK240 pKa = 10.82 KK241 pKa = 9.29 MYY243 pKa = 10.66 HH244 pKa = 6.38 PMNKK248 pKa = 10.02 NIVYY252 pKa = 10.49 DD253 pKa = 4.11 DD254 pKa = 4.79 DD255 pKa = 4.52 EE256 pKa = 6.64 SGTSEE261 pKa = 3.89 TTSYY265 pKa = 11.5 YY266 pKa = 10.97 SVDD269 pKa = 3.49 SKK271 pKa = 11.82 AGMGDD276 pKa = 3.78 YY277 pKa = 10.33 YY278 pKa = 11.29 VWDD281 pKa = 4.18 IFTSGPGGTATDD293 pKa = 4.87 LLRR296 pKa = 11.84 CDD298 pKa = 4.3 ISSTLYY304 pKa = 9.11 WHH306 pKa = 7.03 EE307 pKa = 4.1 KK308 pKa = 9.32
Molecular weight: 34.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.28
IPC2_protein 9.648
IPC_protein 10.072
Toseland 10.204
ProMoST 9.97
Dawson 10.409
Bjellqvist 10.116
Wikipedia 10.613
Rodwell 10.73
Grimsley 10.496
Solomon 10.438
Lehninger 10.409
Nozaki 10.189
DTASelect 10.116
Thurlkill 10.262
EMBOSS 10.613
Sillero 10.321
Patrickios 10.262
IPC_peptide 10.438
IPC2_peptide 8.931
IPC2.peptide.svr19 8.683
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
650
308
342
325.0
36.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.462 ± 0.592
1.538 ± 0.354
6.615 ± 0.688
4.154 ± 1.181
3.846 ± 0.58
9.538 ± 0.689
2.0 ± 0.644
4.923 ± 0.441
4.769 ± 0.879
6.615 ± 0.484
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.308 ± 0.386
3.846 ± 0.643
4.769 ± 0.752
2.462 ± 0.493
8.615 ± 0.503
7.538 ± 1.587
6.923 ± 2.381
5.692 ± 0.516
2.769 ± 0.312
4.615 ± 0.364
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here