Escherichia phage vB_EcoM_ECOO78

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Jilinvirus; Escherichia virus ECOO78

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JT50|A0A1W6JT50_9CAUD Probable tape measure protein OS=Escherichia phage vB_EcoM_ECOO78 OX=1970797 GN=vBEcoMECOO78_36 PE=3 SV=1
MM1 pKa = 7.63AGAFTVVNLSQLPAPNVVVPLDD23 pKa = 3.62FEE25 pKa = 4.88TILAAMIADD34 pKa = 3.99LQDD37 pKa = 3.76RR38 pKa = 11.84DD39 pKa = 3.87PTFTALVEE47 pKa = 4.07SDD49 pKa = 3.52PAYY52 pKa = 10.04KK53 pKa = 9.89ILEE56 pKa = 4.05VCAYY60 pKa = 10.4RR61 pKa = 11.84EE62 pKa = 3.75LLLRR66 pKa = 11.84QRR68 pKa = 11.84VNEE71 pKa = 3.86AAKK74 pKa = 10.75AVMLAYY80 pKa = 10.3ASGADD85 pKa = 4.22LDD87 pKa = 3.92QLGANVGVKK96 pKa = 10.09RR97 pKa = 11.84QVITPADD104 pKa = 4.03DD105 pKa = 3.51TTVPPTPAEE114 pKa = 4.14MEE116 pKa = 3.93SDD118 pKa = 3.01EE119 pKa = 4.71DD120 pKa = 3.53FRR122 pKa = 11.84ARR124 pKa = 11.84IQLSPEE130 pKa = 4.42GYY132 pKa = 5.83TTAGSEE138 pKa = 4.17GSYY141 pKa = 10.46VFHH144 pKa = 7.21GLGADD149 pKa = 3.64ADD151 pKa = 4.16VKK153 pKa = 10.73DD154 pKa = 3.89IQAISPEE161 pKa = 4.15PGKK164 pKa = 9.5VTVYY168 pKa = 10.39VLSRR172 pKa = 11.84TGDD175 pKa = 3.23GTAPAEE181 pKa = 4.45TITAVANTLNGEE193 pKa = 4.75TIRR196 pKa = 11.84PMTDD200 pKa = 2.46EE201 pKa = 4.19VTVLSANIVSYY212 pKa = 9.44TINAEE217 pKa = 3.95LTLFPGPDD225 pKa = 3.28STVVLQSAIDD235 pKa = 3.57AVTAFAEE242 pKa = 4.25SQRR245 pKa = 11.84RR246 pKa = 11.84IGYY249 pKa = 9.55DD250 pKa = 3.1VTLSGLYY257 pKa = 9.72HH258 pKa = 7.26ALHH261 pKa = 6.05QPGVQNVNLTSPTASLVLGDD281 pKa = 4.56GQASYY286 pKa = 8.98CTAINVTVAGDD297 pKa = 3.48TDD299 pKa = 3.68VV300 pKa = 3.56

Molecular weight: 31.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JT26|A0A1W6JT26_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoM_ECOO78 OX=1970797 GN=vBEcoMECOO78_15 PE=4 SV=1
MM1 pKa = 7.77IKK3 pKa = 10.45PLDD6 pKa = 3.33NWHH9 pKa = 6.77AVRR12 pKa = 11.84ALADD16 pKa = 3.87WYY18 pKa = 10.65AFPQMRR24 pKa = 11.84FLRR27 pKa = 11.84RR28 pKa = 11.84SLLARR33 pKa = 11.84KK34 pKa = 8.99CWALIRR40 pKa = 11.84ADD42 pKa = 3.88RR43 pKa = 11.84LLAEE47 pKa = 4.55RR48 pKa = 11.84DD49 pKa = 3.45KK50 pKa = 11.48VQPP53 pKa = 3.8

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12724

53

781

227.2

25.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.398 ± 0.416

1.14 ± 0.149

6.185 ± 0.25

6.052 ± 0.32

3.301 ± 0.197

8.197 ± 0.308

1.721 ± 0.191

5.391 ± 0.217

5.376 ± 0.33

7.663 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.177

4.417 ± 0.205

4.417 ± 0.267

4.456 ± 0.281

5.981 ± 0.294

5.446 ± 0.328

6.099 ± 0.333

6.916 ± 0.278

1.729 ± 0.122

2.609 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski