Bacteroides sp. OM05-12

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; unclassified Bacteroides

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3429 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A374WAN2|A0A374WAN2_9BACE DNA-binding response regulator OS=Bacteroides sp. OM05-12 OX=2292283 GN=DXB63_07875 PE=4 SV=1
MM1 pKa = 7.36TNYY4 pKa = 10.01EE5 pKa = 4.07IQQHH9 pKa = 5.75IDD11 pKa = 2.76ALYY14 pKa = 9.72RR15 pKa = 11.84DD16 pKa = 4.23LNNVEE21 pKa = 5.05GMDD24 pKa = 3.64EE25 pKa = 3.88EE26 pKa = 4.52TARR29 pKa = 11.84RR30 pKa = 11.84VYY32 pKa = 10.89NVDD35 pKa = 3.48CKK37 pKa = 11.27SEE39 pKa = 4.17IIEE42 pKa = 4.41VIQDD46 pKa = 3.72EE47 pKa = 4.54IDD49 pKa = 3.28TCKK52 pKa = 10.96AIMQPDD58 pKa = 4.26LEE60 pKa = 6.55DD61 pKa = 6.17DD62 pKa = 4.36DD63 pKa = 4.37MDD65 pKa = 4.72YY66 pKa = 11.59DD67 pKa = 4.51ALCEE71 pKa = 4.08VQGLSRR77 pKa = 11.84YY78 pKa = 9.1AA79 pKa = 3.4

Molecular weight: 9.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A374W8N8|A0A374W8N8_9BACE Ion transporter OS=Bacteroides sp. OM05-12 OX=2292283 GN=DXB63_14765 PE=4 SV=1
MM1 pKa = 7.89EE2 pKa = 4.71YY3 pKa = 10.66NVNKK7 pKa = 10.75GIGKK11 pKa = 9.01SVEE14 pKa = 3.74FRR16 pKa = 11.84GLKK19 pKa = 9.33SQYY22 pKa = 11.01LFIFAGGLLSVFVVFIIMYY41 pKa = 8.41MIGIGQWICIGFGLVAASVLVWFTFRR67 pKa = 11.84LNARR71 pKa = 11.84YY72 pKa = 9.82GEE74 pKa = 4.29HH75 pKa = 6.9GLMKK79 pKa = 10.29IMAKK83 pKa = 9.77SRR85 pKa = 11.84HH86 pKa = 5.2PRR88 pKa = 11.84YY89 pKa = 10.21LINRR93 pKa = 11.84RR94 pKa = 11.84NARR97 pKa = 11.84LLFRR101 pKa = 11.84CPKK104 pKa = 9.89RR105 pKa = 11.84KK106 pKa = 8.96EE107 pKa = 3.64AAHH110 pKa = 6.23AA111 pKa = 4.04

Molecular weight: 12.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3429

0

3429

1318974

26

3282

384.7

43.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.629 ± 0.042

1.203 ± 0.014

5.678 ± 0.03

6.637 ± 0.043

4.592 ± 0.028

6.706 ± 0.042

1.688 ± 0.017

7.508 ± 0.041

6.998 ± 0.046

8.896 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.635 ± 0.019

5.569 ± 0.034

3.548 ± 0.019

3.276 ± 0.022

4.1 ± 0.03

6.374 ± 0.035

5.791 ± 0.04

6.313 ± 0.03

1.2 ± 0.015

4.659 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski