Streptococcus satellite phage Javan277

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZHS9|A0A4D5ZHS9_9VIRU Terminase small subunit OS=Streptococcus satellite phage Javan277 OX=2558601 GN=JavanS277_0003 PE=4 SV=1
MM1 pKa = 7.64GEE3 pKa = 3.9LGKK6 pKa = 8.28TQGNFDD12 pKa = 4.15FNIVIEE18 pKa = 4.24EE19 pKa = 3.93EE20 pKa = 4.03GDD22 pKa = 3.72YY23 pKa = 11.38VFYY26 pKa = 10.89FSFNDD31 pKa = 3.58EE32 pKa = 4.49NNQSLDD38 pKa = 2.77TYY40 pKa = 8.74YY41 pKa = 10.91QYY43 pKa = 11.42ISIIKK48 pKa = 8.95EE49 pKa = 3.8

Molecular weight: 5.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZIA4|A0A4D5ZIA4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan277 OX=2558601 GN=JavanS277_0009 PE=4 SV=1
MM1 pKa = 7.28VFDD4 pKa = 4.0MRR6 pKa = 11.84QHH8 pKa = 6.19RR9 pKa = 11.84RR10 pKa = 11.84FLNFPTLGNLVVVDD24 pKa = 5.03DD25 pKa = 4.83LLSSEE30 pKa = 4.15RR31 pKa = 11.84TPRR34 pKa = 11.84KK35 pKa = 8.49YY36 pKa = 9.19TIKK39 pKa = 10.36II40 pKa = 3.53

Molecular weight: 4.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

3522

40

381

146.8

17.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.94 ± 0.384

0.738 ± 0.137

5.451 ± 0.398

7.95 ± 0.734

4.174 ± 0.322

4.373 ± 0.342

1.988 ± 0.306

8.404 ± 0.402

9.398 ± 0.422

10.42 ± 0.721

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.924 ± 0.309

5.423 ± 0.461

2.328 ± 0.222

4.571 ± 0.38

4.259 ± 0.449

6.161 ± 0.309

6.161 ± 0.467

4.94 ± 0.405

0.965 ± 0.203

4.429 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski