Salmonella phage SI8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; unclassified Lederbergvirus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C0CAQ8|A0A5C0CAQ8_9CAUD Regulatory protein Cro OS=Salmonella phage SI8 OX=2592213 PE=4 SV=1
MM1 pKa = 7.26LHH3 pKa = 7.23DD4 pKa = 4.7FDD6 pKa = 6.33DD7 pKa = 4.44EE8 pKa = 4.22EE9 pKa = 5.2FIALISPEE17 pKa = 3.74IEE19 pKa = 3.94EE20 pKa = 4.61EE21 pKa = 4.24VEE23 pKa = 4.03QQINLAAEE31 pKa = 4.07RR32 pKa = 11.84QNPVISWDD40 pKa = 3.48EE41 pKa = 3.67FAGYY45 pKa = 10.62YY46 pKa = 9.62SS47 pKa = 3.45

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C0CAT4|A0A5C0CAT4_9CAUD Nin protein OS=Salmonella phage SI8 OX=2592213 PE=4 SV=1
MM1 pKa = 7.54RR2 pKa = 11.84RR3 pKa = 11.84QRR5 pKa = 11.84RR6 pKa = 11.84SITDD10 pKa = 4.1IICEE14 pKa = 3.96NCKK17 pKa = 10.21YY18 pKa = 10.77LPTKK22 pKa = 10.48RR23 pKa = 11.84SRR25 pKa = 11.84NKK27 pKa = 9.87PKK29 pKa = 9.99PAPNEE34 pKa = 4.01SDD36 pKa = 3.45VKK38 pKa = 9.34TFNYY42 pKa = 7.51TAHH45 pKa = 6.73LWDD48 pKa = 4.0IRR50 pKa = 11.84WLRR53 pKa = 11.84YY54 pKa = 8.87RR55 pKa = 11.84ARR57 pKa = 11.84KK58 pKa = 8.72

Molecular weight: 7.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

11955

41

725

189.8

21.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.076 ± 0.533

1.154 ± 0.149

5.931 ± 0.264

6.893 ± 0.369

3.739 ± 0.299

7.143 ± 0.379

1.782 ± 0.176

6.257 ± 0.422

6.19 ± 0.245

7.553 ± 0.312

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.535 ± 0.134

4.86 ± 0.308

3.756 ± 0.186

4.835 ± 0.393

6.023 ± 0.265

6.19 ± 0.271

5.178 ± 0.298

5.688 ± 0.291

1.606 ± 0.15

3.614 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski