Amino acid dipepetide frequency for Streptomyces phage mu1/6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
24.078AlaAla: 24.078 ± 2.306
0.953AlaCys: 0.953 ± 0.426
9.007AlaAsp: 9.007 ± 0.722
10.393AlaGlu: 10.393 ± 0.978
2.425AlaPhe: 2.425 ± 0.463
11.433AlaGly: 11.433 ± 1.417
3.205AlaHis: 3.205 ± 0.77
4.417AlaIle: 4.417 ± 0.678
2.685AlaLys: 2.685 ± 0.504
12.385AlaLeu: 12.385 ± 1.002
2.685AlaMet: 2.685 ± 0.598
2.772AlaAsn: 2.772 ± 0.451
8.141AlaPro: 8.141 ± 0.627
6.409AlaGln: 6.409 ± 1.139
10.48AlaArg: 10.48 ± 1.03
6.149AlaSer: 6.149 ± 1.03
8.401AlaThr: 8.401 ± 0.989
10.566AlaVal: 10.566 ± 1.258
2.252AlaTrp: 2.252 ± 0.458
3.118AlaTyr: 3.118 ± 0.409
0.0AlaXaa: 0.0 ± 0.0
Cys
1.039CysAla: 1.039 ± 0.419
0.173CysCys: 0.173 ± 0.187
0.866CysAsp: 0.866 ± 0.293
0.087CysGlu: 0.087 ± 0.086
0.087CysPhe: 0.087 ± 0.078
1.213CysGly: 1.213 ± 0.446
0.173CysHis: 0.173 ± 0.123
0.26CysIle: 0.26 ± 0.152
0.26CysLys: 0.26 ± 0.145
0.173CysLeu: 0.173 ± 0.12
0.173CysMet: 0.173 ± 0.106
0.0CysAsn: 0.0 ± 0.0
0.866CysPro: 0.866 ± 0.343
0.087CysGln: 0.087 ± 0.078
0.953CysArg: 0.953 ± 0.383
0.433CysSer: 0.433 ± 0.182
0.866CysThr: 0.866 ± 0.316
0.52CysVal: 0.52 ± 0.221
0.173CysTrp: 0.173 ± 0.134
0.087CysTyr: 0.087 ± 0.087
0.0CysXaa: 0.0 ± 0.0
Asp
9.44AspAla: 9.44 ± 1.093
0.779AspCys: 0.779 ± 0.311
3.205AspAsp: 3.205 ± 0.668
2.945AspGlu: 2.945 ± 0.742
0.606AspPhe: 0.606 ± 0.281
6.669AspGly: 6.669 ± 0.725
1.126AspHis: 1.126 ± 0.333
1.646AspIle: 1.646 ± 0.269
1.472AspLys: 1.472 ± 0.321
5.456AspLeu: 5.456 ± 0.685
1.299AspMet: 1.299 ± 0.286
0.866AspAsn: 0.866 ± 0.264
4.59AspPro: 4.59 ± 0.828
3.118AspGln: 3.118 ± 0.538
5.716AspArg: 5.716 ± 0.552
2.079AspSer: 2.079 ± 0.522
4.157AspThr: 4.157 ± 0.633
4.331AspVal: 4.331 ± 0.54
1.472AspTrp: 1.472 ± 0.339
1.213AspTyr: 1.213 ± 0.241
0.0AspXaa: 0.0 ± 0.0
Glu
5.976GluAla: 5.976 ± 0.653
0.087GluCys: 0.087 ± 0.076
1.819GluAsp: 1.819 ± 0.421
2.512GluGlu: 2.512 ± 0.436
1.299GluPhe: 1.299 ± 0.325
2.858GluGly: 2.858 ± 0.45
0.866GluHis: 0.866 ± 0.251
2.512GluIle: 2.512 ± 0.486
1.126GluLys: 1.126 ± 0.328
6.669GluLeu: 6.669 ± 0.79
1.126GluMet: 1.126 ± 0.267
1.299GluAsn: 1.299 ± 0.425
1.992GluPro: 1.992 ± 0.477
3.811GluGln: 3.811 ± 0.511
4.764GluArg: 4.764 ± 0.671
1.213GluSer: 1.213 ± 0.362
3.378GluThr: 3.378 ± 0.546
3.811GluVal: 3.811 ± 0.697
0.779GluTrp: 0.779 ± 0.339
1.213GluTyr: 1.213 ± 0.336
0.0GluXaa: 0.0 ± 0.0
Phe
2.338PheAla: 2.338 ± 0.352
0.087PheCys: 0.087 ± 0.098
1.819PheAsp: 1.819 ± 0.402
0.953PheGlu: 0.953 ± 0.295
0.26PhePhe: 0.26 ± 0.137
1.819PheGly: 1.819 ± 0.481
0.173PheHis: 0.173 ± 0.123
0.779PheIle: 0.779 ± 0.265
0.346PheLys: 0.346 ± 0.178
1.213PheLeu: 1.213 ± 0.303
0.52PheMet: 0.52 ± 0.205
0.433PheAsn: 0.433 ± 0.247
0.779PhePro: 0.779 ± 0.272
0.52PheGln: 0.52 ± 0.176
1.905PheArg: 1.905 ± 0.365
0.779PheSer: 0.779 ± 0.242
1.819PheThr: 1.819 ± 0.471
1.213PheVal: 1.213 ± 0.357
0.087PheTrp: 0.087 ± 0.078
0.26PheTyr: 0.26 ± 0.119
0.0PheXaa: 0.0 ± 0.0
Gly
10.74GlyAla: 10.74 ± 1.14
0.52GlyCys: 0.52 ± 0.214
4.764GlyAsp: 4.764 ± 0.581
3.118GlyGlu: 3.118 ± 0.534
2.079GlyPhe: 2.079 ± 0.397
7.102GlyGly: 7.102 ± 0.737
2.425GlyHis: 2.425 ± 0.51
4.59GlyIle: 4.59 ± 0.556
1.732GlyLys: 1.732 ± 0.345
6.149GlyLeu: 6.149 ± 0.78
1.646GlyMet: 1.646 ± 0.397
1.992GlyAsn: 1.992 ± 0.432
5.456GlyPro: 5.456 ± 0.61
4.59GlyGln: 4.59 ± 0.662
5.543GlyArg: 5.543 ± 0.79
6.669GlySer: 6.669 ± 0.901
7.015GlyThr: 7.015 ± 0.922
6.236GlyVal: 6.236 ± 0.762
1.732GlyTrp: 1.732 ± 0.569
1.992GlyTyr: 1.992 ± 0.464
0.0GlyXaa: 0.0 ± 0.0
His
2.685HisAla: 2.685 ± 0.484
0.26HisCys: 0.26 ± 0.143
1.126HisAsp: 1.126 ± 0.356
0.953HisGlu: 0.953 ± 0.272
0.346HisPhe: 0.346 ± 0.178
1.299HisGly: 1.299 ± 0.393
1.213HisHis: 1.213 ± 0.316
0.606HisIle: 0.606 ± 0.241
0.087HisLys: 0.087 ± 0.098
2.079HisLeu: 2.079 ± 0.488
0.433HisMet: 0.433 ± 0.184
0.346HisAsn: 0.346 ± 0.153
2.252HisPro: 2.252 ± 0.457
0.866HisGln: 0.866 ± 0.311
2.338HisArg: 2.338 ± 0.725
0.779HisSer: 0.779 ± 0.251
1.646HisThr: 1.646 ± 0.384
1.992HisVal: 1.992 ± 0.35
0.346HisTrp: 0.346 ± 0.155
0.346HisTyr: 0.346 ± 0.175
0.0HisXaa: 0.0 ± 0.0
Ile
5.456IleAla: 5.456 ± 0.676
0.173IleCys: 0.173 ± 0.115
2.598IleAsp: 2.598 ± 0.543
2.338IleGlu: 2.338 ± 0.457
0.087IlePhe: 0.087 ± 0.08
3.984IleGly: 3.984 ± 0.453
0.606IleHis: 0.606 ± 0.219
1.472IleIle: 1.472 ± 0.448
0.693IleLys: 0.693 ± 0.276
3.205IleLeu: 3.205 ± 0.505
0.606IleMet: 0.606 ± 0.226
1.213IleAsn: 1.213 ± 0.387
2.512IlePro: 2.512 ± 0.565
1.213IleGln: 1.213 ± 0.297
3.118IleArg: 3.118 ± 0.545
1.646IleSer: 1.646 ± 0.321
4.244IleThr: 4.244 ± 0.683
3.291IleVal: 3.291 ± 0.528
0.693IleTrp: 0.693 ± 0.226
0.433IleTyr: 0.433 ± 0.257
0.0IleXaa: 0.0 ± 0.0
Lys
2.772LysAla: 2.772 ± 0.499
0.087LysCys: 0.087 ± 0.081
1.126LysAsp: 1.126 ± 0.31
0.693LysGlu: 0.693 ± 0.341
0.26LysPhe: 0.26 ± 0.223
2.772LysGly: 2.772 ± 0.468
0.26LysHis: 0.26 ± 0.133
1.039LysIle: 1.039 ± 0.287
0.52LysLys: 0.52 ± 0.29
0.953LysLeu: 0.953 ± 0.291
0.26LysMet: 0.26 ± 0.162
0.173LysAsn: 0.173 ± 0.113
1.126LysPro: 1.126 ± 0.37
0.953LysGln: 0.953 ± 0.295
1.992LysArg: 1.992 ± 0.412
0.866LysSer: 0.866 ± 0.279
1.905LysThr: 1.905 ± 0.449
1.646LysVal: 1.646 ± 0.41
0.346LysTrp: 0.346 ± 0.16
0.52LysTyr: 0.52 ± 0.203
0.0LysXaa: 0.0 ± 0.0
Leu
14.117LeuAla: 14.117 ± 1.633
0.779LeuCys: 0.779 ± 0.298
7.448LeuAsp: 7.448 ± 0.803
3.464LeuGlu: 3.464 ± 0.683
1.299LeuPhe: 1.299 ± 0.291
6.929LeuGly: 6.929 ± 0.769
2.079LeuHis: 2.079 ± 0.379
2.512LeuIle: 2.512 ± 0.478
1.472LeuLys: 1.472 ± 0.368
8.228LeuLeu: 8.228 ± 1.005
1.992LeuMet: 1.992 ± 0.362
1.732LeuAsn: 1.732 ± 0.369
6.496LeuPro: 6.496 ± 0.832
3.118LeuGln: 3.118 ± 0.425
7.102LeuArg: 7.102 ± 0.766
4.764LeuSer: 4.764 ± 0.76
5.63LeuThr: 5.63 ± 0.866
5.976LeuVal: 5.976 ± 0.628
1.386LeuTrp: 1.386 ± 0.393
1.472LeuTyr: 1.472 ± 0.288
0.0LeuXaa: 0.0 ± 0.0
Met
2.945MetAla: 2.945 ± 0.453
0.26MetCys: 0.26 ± 0.145
1.213MetAsp: 1.213 ± 0.275
0.606MetGlu: 0.606 ± 0.34
0.433MetPhe: 0.433 ± 0.186
1.299MetGly: 1.299 ± 0.301
0.26MetHis: 0.26 ± 0.137
0.26MetIle: 0.26 ± 0.134
0.173MetLys: 0.173 ± 0.109
1.039MetLeu: 1.039 ± 0.434
0.173MetMet: 0.173 ± 0.12
0.606MetAsn: 0.606 ± 0.228
2.338MetPro: 2.338 ± 0.334
1.126MetGln: 1.126 ± 0.288
1.646MetArg: 1.646 ± 0.295
1.819MetSer: 1.819 ± 0.452
1.905MetThr: 1.905 ± 0.341
1.299MetVal: 1.299 ± 0.27
0.173MetTrp: 0.173 ± 0.107
0.433MetTyr: 0.433 ± 0.173
0.0MetXaa: 0.0 ± 0.0
Asn
1.992AsnAla: 1.992 ± 0.562
0.173AsnCys: 0.173 ± 0.108
0.693AsnAsp: 0.693 ± 0.217
1.039AsnGlu: 1.039 ± 0.323
0.346AsnPhe: 0.346 ± 0.211
3.464AsnGly: 3.464 ± 0.523
0.346AsnHis: 0.346 ± 0.197
0.693AsnIle: 0.693 ± 0.286
0.52AsnLys: 0.52 ± 0.181
1.126AsnLeu: 1.126 ± 0.336
0.433AsnMet: 0.433 ± 0.174
0.866AsnAsn: 0.866 ± 0.232
1.299AsnPro: 1.299 ± 0.374
0.606AsnGln: 0.606 ± 0.216
1.646AsnArg: 1.646 ± 0.299
1.213AsnSer: 1.213 ± 0.322
1.472AsnThr: 1.472 ± 0.331
1.646AsnVal: 1.646 ± 0.479
0.173AsnTrp: 0.173 ± 0.128
0.866AsnTyr: 0.866 ± 0.297
0.0AsnXaa: 0.0 ± 0.0
Pro
11.346ProAla: 11.346 ± 1.247
1.126ProCys: 1.126 ± 0.483
5.543ProAsp: 5.543 ± 0.66
3.811ProGlu: 3.811 ± 0.717
0.779ProPhe: 0.779 ± 0.237
6.669ProGly: 6.669 ± 0.963
1.126ProHis: 1.126 ± 0.363
2.338ProIle: 2.338 ± 0.371
1.472ProLys: 1.472 ± 0.35
5.11ProLeu: 5.11 ± 0.619
1.472ProMet: 1.472 ± 0.367
1.126ProAsn: 1.126 ± 0.344
4.937ProPro: 4.937 ± 1.043
2.945ProGln: 2.945 ± 0.47
3.638ProArg: 3.638 ± 0.728
3.205ProSer: 3.205 ± 0.435
4.937ProThr: 4.937 ± 0.857
5.11ProVal: 5.11 ± 0.64
1.126ProTrp: 1.126 ± 0.294
1.299ProTyr: 1.299 ± 0.359
0.0ProXaa: 0.0 ± 0.0
Gln
8.488GlnAla: 8.488 ± 1.231
0.346GlnCys: 0.346 ± 0.149
2.165GlnAsp: 2.165 ± 0.47
1.646GlnGlu: 1.646 ± 0.435
0.953GlnPhe: 0.953 ± 0.289
3.291GlnGly: 3.291 ± 0.611
0.953GlnHis: 0.953 ± 0.285
2.338GlnIle: 2.338 ± 0.485
0.953GlnLys: 0.953 ± 0.282
5.63GlnLeu: 5.63 ± 0.632
1.213GlnMet: 1.213 ± 0.331
0.866GlnAsn: 0.866 ± 0.241
3.205GlnPro: 3.205 ± 0.646
4.071GlnGln: 4.071 ± 0.719
3.378GlnArg: 3.378 ± 0.545
1.386GlnSer: 1.386 ± 0.361
1.472GlnThr: 1.472 ± 0.346
3.897GlnVal: 3.897 ± 0.625
0.779GlnTrp: 0.779 ± 0.271
0.953GlnTyr: 0.953 ± 0.342
0.0GlnXaa: 0.0 ± 0.0
Arg
9.874ArgAla: 9.874 ± 1.108
1.126ArgCys: 1.126 ± 0.296
4.504ArgAsp: 4.504 ± 0.705
3.031ArgGlu: 3.031 ± 0.52
1.386ArgPhe: 1.386 ± 0.297
4.85ArgGly: 4.85 ± 0.786
2.425ArgHis: 2.425 ± 0.573
4.331ArgIle: 4.331 ± 0.701
2.079ArgLys: 2.079 ± 0.523
7.882ArgLeu: 7.882 ± 0.991
1.732ArgMet: 1.732 ± 0.33
0.953ArgAsn: 0.953 ± 0.274
5.37ArgPro: 5.37 ± 0.847
3.205ArgGln: 3.205 ± 0.558
6.063ArgArg: 6.063 ± 1.219
2.945ArgSer: 2.945 ± 0.595
6.149ArgThr: 6.149 ± 0.684
4.937ArgVal: 4.937 ± 0.599
1.299ArgTrp: 1.299 ± 0.402
1.905ArgTyr: 1.905 ± 0.509
0.0ArgXaa: 0.0 ± 0.0
Ser
7.015SerAla: 7.015 ± 1.105
0.26SerCys: 0.26 ± 0.177
2.338SerAsp: 2.338 ± 0.385
1.472SerGlu: 1.472 ± 0.343
1.386SerPhe: 1.386 ± 0.293
4.677SerGly: 4.677 ± 0.641
0.779SerHis: 0.779 ± 0.266
1.905SerIle: 1.905 ± 0.406
1.299SerLys: 1.299 ± 0.445
4.677SerLeu: 4.677 ± 0.727
1.126SerMet: 1.126 ± 0.356
0.693SerAsn: 0.693 ± 0.193
2.512SerPro: 2.512 ± 0.365
2.165SerGln: 2.165 ± 0.375
2.945SerArg: 2.945 ± 0.508
2.252SerSer: 2.252 ± 0.561
3.811SerThr: 3.811 ± 0.615
3.378SerVal: 3.378 ± 0.526
0.693SerTrp: 0.693 ± 0.254
0.953SerTyr: 0.953 ± 0.292
0.0SerXaa: 0.0 ± 0.0
Thr
8.921ThrAla: 8.921 ± 1.059
0.693ThrCys: 0.693 ± 0.281
4.85ThrAsp: 4.85 ± 0.763
3.638ThrGlu: 3.638 ± 0.59
1.905ThrPhe: 1.905 ± 0.45
6.756ThrGly: 6.756 ± 0.727
1.559ThrHis: 1.559 ± 0.423
3.551ThrIle: 3.551 ± 0.483
1.039ThrLys: 1.039 ± 0.321
5.803ThrLeu: 5.803 ± 0.767
0.866ThrMet: 0.866 ± 0.327
1.213ThrAsn: 1.213 ± 0.334
6.669ThrPro: 6.669 ± 0.84
1.559ThrGln: 1.559 ± 0.33
3.984ThrArg: 3.984 ± 0.606
3.031ThrSer: 3.031 ± 0.402
5.63ThrThr: 5.63 ± 0.728
5.023ThrVal: 5.023 ± 0.605
1.992ThrTrp: 1.992 ± 0.408
2.685ThrTyr: 2.685 ± 0.379
0.0ThrXaa: 0.0 ± 0.0
Val
8.228ValAla: 8.228 ± 1.09
0.26ValCys: 0.26 ± 0.143
4.244ValAsp: 4.244 ± 0.504
4.504ValGlu: 4.504 ± 0.481
1.732ValPhe: 1.732 ± 0.289
5.543ValGly: 5.543 ± 0.791
1.472ValHis: 1.472 ± 0.375
2.772ValIle: 2.772 ± 0.379
1.386ValLys: 1.386 ± 0.27
6.409ValLeu: 6.409 ± 0.742
1.646ValMet: 1.646 ± 0.4
2.165ValAsn: 2.165 ± 0.466
5.716ValPro: 5.716 ± 0.605
4.937ValGln: 4.937 ± 1.089
5.11ValArg: 5.11 ± 0.622
3.464ValSer: 3.464 ± 0.524
4.59ValThr: 4.59 ± 0.835
5.023ValVal: 5.023 ± 0.716
1.646ValTrp: 1.646 ± 0.436
1.386ValTyr: 1.386 ± 0.358
0.0ValXaa: 0.0 ± 0.0
Trp
2.165TrpAla: 2.165 ± 0.525
0.087TrpCys: 0.087 ± 0.071
1.213TrpAsp: 1.213 ± 0.355
0.779TrpGlu: 0.779 ± 0.21
0.173TrpPhe: 0.173 ± 0.115
1.126TrpGly: 1.126 ± 0.344
0.433TrpHis: 0.433 ± 0.179
0.606TrpIle: 0.606 ± 0.208
0.52TrpLys: 0.52 ± 0.156
2.079TrpLeu: 2.079 ± 0.431
0.433TrpMet: 0.433 ± 0.202
0.779TrpAsn: 0.779 ± 0.257
1.213TrpPro: 1.213 ± 0.333
1.386TrpGln: 1.386 ± 0.346
0.779TrpArg: 0.779 ± 0.267
0.953TrpSer: 0.953 ± 0.295
1.213TrpThr: 1.213 ± 0.306
0.866TrpVal: 0.866 ± 0.25
0.52TrpTrp: 0.52 ± 0.23
0.779TrpTyr: 0.779 ± 0.315
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.338TyrAla: 2.338 ± 0.438
0.26TyrCys: 0.26 ± 0.127
1.732TyrAsp: 1.732 ± 0.388
1.126TyrGlu: 1.126 ± 0.365
0.26TyrPhe: 0.26 ± 0.156
2.338TyrGly: 2.338 ± 0.435
0.52TyrHis: 0.52 ± 0.198
0.953TyrIle: 0.953 ± 0.252
0.433TyrLys: 0.433 ± 0.221
1.732TyrLeu: 1.732 ± 0.312
0.087TyrMet: 0.087 ± 0.087
0.52TyrAsn: 0.52 ± 0.192
1.646TyrPro: 1.646 ± 0.408
1.299TyrGln: 1.299 ± 0.431
2.945TyrArg: 2.945 ± 0.601
0.693TyrSer: 0.693 ± 0.239
1.126TyrThr: 1.126 ± 0.307
1.472TyrVal: 1.472 ± 0.353
0.433TyrTrp: 0.433 ± 0.184
0.52TyrTyr: 0.52 ± 0.22
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (11547 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski